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The map label for this gene is slt [H]

Identifier: 66046524

GI number: 66046524

Start: 3942828

End: 3944756

Strand: Direct

Name: slt [H]

Synonym: Psyr_3295

Alternate gene names: 66046524

Gene position: 3942828-3944756 (Clockwise)

Preceding gene: 66046522

Following gene: 66046527

Centisome position: 64.7

GC content: 61.43

Gene sequence:

>1929_bases
ATGCGCAGTCGTTTGTTCAGCCTTTTATCGTGCCTGCTCCTCTCCGCCACCGCCGTCCAGACTGCTCATGCGGTAGACCT
CAACCAACAACGCAAATATTACGACGAAGCAAAGCGCGCACTGGCCAAAGGCGATTCCGGCCCCTACCAGATGTACGCCA
CGGCCCTGGCCGATTATCCGCTGGAGCCTTACCTCGAATACGACGAACTGACGGCACGCCTGAAAACGGCAAGCAATCCC
GAAATCGAGAAGTTTCTTGCCGAGCACGGCGACCTGCCGCAAGCCAACTGGATGAAGCTGCGCTGGTTGCGCTGGCTGGC
CGAGCGTGGCGACTGGCAAACCTTCGTCAAGTATTACGACCCGAAGATGAAGTTCGTCGAGCTGGACTGCCTGTACGGGC
AGTATCAGCTGAACAGCAACAAGCGCGCCGAAGGCTTCGCCAGTGCCGAAAAGCTGTGGATGACCGGCAAGACTTTACCC
GCTGCCTGTGACGGTTTCTTTGCCCAGTGGGCGGCCGCCGGCCAGTTGACCGAACAGAAGCGCTGGCAGCGCGCCAAGCT
GGCGGCACAGACGCGCAATTACAGTCTGGCCAGCACGCTGGTGGACAGCCTCAACTCGCTGGCACCGCAAGGCCGTCTGC
TGCTGGCTGTCGCGCAGAAACCGGAGATGGTCAGCAACCCGTCGCAATTTGCCCCGGTAGACGAGGCGATGTCCGATGTG
GTCGGTCTCGGCCTGCGCCGGCTCGCCAAGCAGGACCCGCAGAAAGCCCTGTCGATGCTCGATGGCTATGCCGCGACCAT
GCATTTCTCCCGCGAAGAGCAGGTCGAGATTGCCAAGGAAATCGGCCTGACCCTGGCACGTCGTTATGACGACCGCGCAC
TGGAGGTCATGACCAGATACGACCCTGAATTGCGTGACGACACCGTGACCGAATGGCGCCTGCGCCTGCTGCTGCGCCTG
GGTCGCTGGGAAGACGCCTACGAGCTGGCGCGCCGACTGCCCAAGGATCTGGCAGCGACCAATCGCTGGCGTTACTGGGA
AGCGCGCTCTCTGGAACTGGCACAACCCAACAGCCCGCTGATTGCCGCACTGTACAAGGATGTCGCCAAGGAGCGTGATT
TCTACGGGTTTCTGGCTGCCGACCGCACTCAAAGCCCCTACCAGCTCAACAACAAGCCGCTGGTGCTGAGCCAGGCACTG
ATCAACAAGGTACGCAACACGCCGGGCGTGCGTCGCGCACTGGAGTTTCACGATCGCGGCGAGATCGTCAACGGCCGACG
CGAGTGGTATCACGTGAGCCGCCTGTTCAATCGTGACGAGATGGTTGCCCAGGCCAAGCTGGCCTATGACATGCAATGGT
ATTTCCCGGCAATCCGTACCATCAGCCAGGCGCAATACTGGGACGATCTGGATATTCGCTTCCCGATGGCGCACCGCGAC
ACGCTGGTGCGCGAAGCCAAGGTGCGCGGCCTGCATTCGAGCTGGGTGTTCGCCATCACGCGCCAGGAAAGCGCCTTCAT
GGACGATGCCCGCTCGGGCGTCGGCGCCAGCGGGCTGATGCAACTGATGCCTGCCACCGCCAAGGAGACTGCGCGCAAAT
ACAGCATCCCGCTGGCCTCGCCTCAGCAGGTGCTGGATCCGGACACCAACATCCAGCTGGGCGCCGCCTACCTGAGCTCG
GTGCATGCGCAGTTCAACGGTAATCGGGTGCTCGCTTCAGCGGCCTACAACGCAGGTCCCGGGCGCGTGCGCCAATGGCT
CAAAGGTGCCAACCATCTGGCCTTCGATGTCTGGGTAGAGAGCATTCCGTTCGATGAGACCCGTCAGTACGTACAGAACG
TATTGTCGTACTCGGTGATCTACGGCCAGAAACTCAACTCGCCGCAGCCTCTGGTGGACTGGCACGAGCGGTTTTTCGAC
GACCAGTGA

Upstream 100 bases:

>100_bases
ATTTCCATATGAGACAAGCTCGTCGGCAATGCTTTCACCAAGAGCTGGCAAAAGGCTAAGCTAGCAATACTTTTCACCGA
CTCATCTGCTCGGAAGTCTC

Downstream 100 bases:

>100_bases
CCGGTCGGCAGGAGTGCTGCGTTAAGCACTCCTGCCAGACAACGGCAGTCCCCGCCCCTACTCCAGCACTTCGACTGACA
TCCCCACTTCAAGCTCGCCC

Product: SLT

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MRSRLFSLLSCLLLSATAVQTAHAVDLNQQRKYYDEAKRALAKGDSGPYQMYATALADYPLEPYLEYDELTARLKTASNP
EIEKFLAEHGDLPQANWMKLRWLRWLAERGDWQTFVKYYDPKMKFVELDCLYGQYQLNSNKRAEGFASAEKLWMTGKTLP
AACDGFFAQWAAAGQLTEQKRWQRAKLAAQTRNYSLASTLVDSLNSLAPQGRLLLAVAQKPEMVSNPSQFAPVDEAMSDV
VGLGLRRLAKQDPQKALSMLDGYAATMHFSREEQVEIAKEIGLTLARRYDDRALEVMTRYDPELRDDTVTEWRLRLLLRL
GRWEDAYELARRLPKDLAATNRWRYWEARSLELAQPNSPLIAALYKDVAKERDFYGFLAADRTQSPYQLNNKPLVLSQAL
INKVRNTPGVRRALEFHDRGEIVNGRREWYHVSRLFNRDEMVAQAKLAYDMQWYFPAIRTISQAQYWDDLDIRFPMAHRD
TLVREAKVRGLHSSWVFAITRQESAFMDDARSGVGASGLMQLMPATAKETARKYSIPLASPQQVLDPDTNIQLGAAYLSS
VHAQFNGNRVLASAAYNAGPGRVRQWLKGANHLAFDVWVESIPFDETRQYVQNVLSYSVIYGQKLNSPQPLVDWHERFFD
DQ

Sequences:

>Translated_642_residues
MRSRLFSLLSCLLLSATAVQTAHAVDLNQQRKYYDEAKRALAKGDSGPYQMYATALADYPLEPYLEYDELTARLKTASNP
EIEKFLAEHGDLPQANWMKLRWLRWLAERGDWQTFVKYYDPKMKFVELDCLYGQYQLNSNKRAEGFASAEKLWMTGKTLP
AACDGFFAQWAAAGQLTEQKRWQRAKLAAQTRNYSLASTLVDSLNSLAPQGRLLLAVAQKPEMVSNPSQFAPVDEAMSDV
VGLGLRRLAKQDPQKALSMLDGYAATMHFSREEQVEIAKEIGLTLARRYDDRALEVMTRYDPELRDDTVTEWRLRLLLRL
GRWEDAYELARRLPKDLAATNRWRYWEARSLELAQPNSPLIAALYKDVAKERDFYGFLAADRTQSPYQLNNKPLVLSQAL
INKVRNTPGVRRALEFHDRGEIVNGRREWYHVSRLFNRDEMVAQAKLAYDMQWYFPAIRTISQAQYWDDLDIRFPMAHRD
TLVREAKVRGLHSSWVFAITRQESAFMDDARSGVGASGLMQLMPATAKETARKYSIPLASPQQVLDPDTNIQLGAAYLSS
VHAQFNGNRVLASAAYNAGPGRVRQWLKGANHLAFDVWVESIPFDETRQYVQNVLSYSVIYGQKLNSPQPLVDWHERFFD
DQ
>Mature_642_residues
MRSRLFSLLSCLLLSATAVQTAHAVDLNQQRKYYDEAKRALAKGDSGPYQMYATALADYPLEPYLEYDELTARLKTASNP
EIEKFLAEHGDLPQANWMKLRWLRWLAERGDWQTFVKYYDPKMKFVELDCLYGQYQLNSNKRAEGFASAEKLWMTGKTLP
AACDGFFAQWAAAGQLTEQKRWQRAKLAAQTRNYSLASTLVDSLNSLAPQGRLLLAVAQKPEMVSNPSQFAPVDEAMSDV
VGLGLRRLAKQDPQKALSMLDGYAATMHFSREEQVEIAKEIGLTLARRYDDRALEVMTRYDPELRDDTVTEWRLRLLLRL
GRWEDAYELARRLPKDLAATNRWRYWEARSLELAQPNSPLIAALYKDVAKERDFYGFLAADRTQSPYQLNNKPLVLSQAL
INKVRNTPGVRRALEFHDRGEIVNGRREWYHVSRLFNRDEMVAQAKLAYDMQWYFPAIRTISQAQYWDDLDIRFPMAHRD
TLVREAKVRGLHSSWVFAITRQESAFMDDARSGVGASGLMQLMPATAKETARKYSIPLASPQQVLDPDTNIQLGAAYLSS
VHAQFNGNRVLASAAYNAGPGRVRQWLKGANHLAFDVWVESIPFDETRQYVQNVLSYSVIYGQKLNSPQPLVDWHERFFD
DQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=625, Percent_Identity=31.52, Blast_Score=292, Evalue=5e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73616; Mature: 73616

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRLFSLLSCLLLSATAVQTAHAVDLNQQRKYYDEAKRALAKGDSGPYQMYATALADYP
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
LEPYLEYDELTARLKTASNPEIEKFLAEHGDLPQANWMKLRWLRWLAERGDWQTFVKYYD
CCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHC
PKMKFVELDCLYGQYQLNSNKRAEGFASAEKLWMTGKTLPAACDGFFAQWAAAGQLTEQK
CCCEEEEEEEEECEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
RWQRAKLAAQTRNYSLASTLVDSLNSLAPQGRLLLAVAQKPEMVSNPSQFAPVDEAMSDV
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHCCCCCCCCHHHHHHHH
VGLGLRRLAKQDPQKALSMLDGYAATMHFSREEQVEIAKEIGLTLARRYDDRALEVMTRY
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHC
DPELRDDTVTEWRLRLLLRLGRWEDAYELARRLPKDLAATNRWRYWEARSLELAQPNSPL
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCCEEEECCCCEECCCCCHH
IAALYKDVAKERDFYGFLAADRTQSPYQLNNKPLVLSQALINKVRNTPGVRRALEFHDRG
HHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
EIVNGRREWYHVSRLFNRDEMVAQAKLAYDMQWYFPAIRTISQAQYWDDLDIRFPMAHRD
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHH
TLVREAKVRGLHSSWVFAITRQESAFMDDARSGVGASGLMQLMPATAKETARKYSIPLAS
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHCCCCCC
PQQVLDPDTNIQLGAAYLSSVHAQFNGNRVLASAAYNAGPGRVRQWLKGANHLAFDVWVE
CHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEHHH
SIPFDETRQYVQNVLSYSVIYGQKLNSPQPLVDWHERFFDDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MRSRLFSLLSCLLLSATAVQTAHAVDLNQQRKYYDEAKRALAKGDSGPYQMYATALADYP
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
LEPYLEYDELTARLKTASNPEIEKFLAEHGDLPQANWMKLRWLRWLAERGDWQTFVKYYD
CCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHC
PKMKFVELDCLYGQYQLNSNKRAEGFASAEKLWMTGKTLPAACDGFFAQWAAAGQLTEQK
CCCEEEEEEEEECEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
RWQRAKLAAQTRNYSLASTLVDSLNSLAPQGRLLLAVAQKPEMVSNPSQFAPVDEAMSDV
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHCCCCCCCCHHHHHHHH
VGLGLRRLAKQDPQKALSMLDGYAATMHFSREEQVEIAKEIGLTLARRYDDRALEVMTRY
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHC
DPELRDDTVTEWRLRLLLRLGRWEDAYELARRLPKDLAATNRWRYWEARSLELAQPNSPL
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCCEEEECCCCEECCCCCHH
IAALYKDVAKERDFYGFLAADRTQSPYQLNNKPLVLSQALINKVRNTPGVRRALEFHDRG
HHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
EIVNGRREWYHVSRLFNRDEMVAQAKLAYDMQWYFPAIRTISQAQYWDDLDIRFPMAHRD
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHH
TLVREAKVRGLHSSWVFAITRQESAFMDDARSGVGASGLMQLMPATAKETARKYSIPLAS
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHCCCCCC
PQQVLDPDTNIQLGAAYLSSVHAQFNGNRVLASAAYNAGPGRVRQWLKGANHLAFDVWVE
CHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEHHH
SIPFDETRQYVQNVLSYSVIYGQKLNSPQPLVDWHERFFDDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]