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The map label for this gene is arnB

Identifier: 66045925

GI number: 66045925

Start: 3265023

End: 3266171

Strand: Direct

Name: arnB

Synonym: Psyr_2689

Alternate gene names: 66045925

Gene position: 3265023-3266171 (Clockwise)

Preceding gene: 66045922

Following gene: 66045926

Centisome position: 53.58

GC content: 60.49

Gene sequence:

>1149_bases
ATGAGTCAAACTTTTCTTGCCTTTTCACGGCCGTCCATTGGCGATGAAGAAATTGCTGCAGTGACGCGCGTGCTGCGTTC
CGGATGGATCACCACCGGACCGGAATGTCAGAAGCTGGAGGAAGAGTTCGCCGCGCGGGTCGGCGCGCGGCATGCAGTGG
CATTGTCTTCGGCCACCGGGGCAATGCACGTTGCGCTGCTGGCGCTGGGTGTAGGGCCTGGCGATGAAGTGATTACGCCG
TCGCAAACCTGGGTGTCGACAGCCAATATGATCTGCCTGCTGGGAGCCACTCCGGTGTTTGTCGATGTCGACCGCGACAC
CCTGATGACCAGCGCCGCGTTGATAGAGCATGCCATCACTCCGCGGACCAAGGCGATCGTGCCAGTGCATTATGCAGGCG
CGGCATTCGATCTTGACCCGCTGTATGCGCTGGCCGACCGGCACGGCATCACCGTGATCGAAGACGCTGCGCATGCTGCC
GGCACTGCTTACCAAGGACGCCCGGTCGGCCAGCAGGGCACTGCGATCTTTTCGTTTCACGCGATCAAGAACATGACCTG
TGCCGAAGGCGCGATGCTGGTCACCGACAATGCACGGCTGGCTGATCGGGTGCGTCAGCTTAAATTCCATGGCCTGGGTG
TCGATGCCTACGATCGCCTGACCCTGGGTCGCAAACCGCAGGCCGAGGTCATGGAGCCTGGCTTCAAATACAACCTGGCC
GACATTAATGCGAGCATTGCGCGGGTGCAGTTGCAGCGCCTCGACGCCATCAATGCACAGCGCCAGGCGCTCGCCAGCCA
TTACCTCGAACGACTGGCCAATAGCCCCGTTCTGCCTCTCGCCCTGCCCCGGTATGCCCAGCAACACGCTTGGCACCTGT
TCATTCTGCGCATTGATCCCGAGCGCTGCGGGCTGGATCGCGATGCCTTCATGAAGGCCTTGCAAGCGCGAAATATCGGC
ACCGGCATCCACTTCATCGCGACCCACCTGCACAGCTATTACCGCAAGCGTTTCCCGGATGTACGCCTGCCCGACACTGA
ATGGAATTCATCACGGCTGTGTTCCATCCCCCTGTTCCCCGACATGAGCCTCGATGATGTCGAGCGGGTTGTCGGTGCCA
TTGAATCGACCCTGGAATCCAGCCATTGA

Upstream 100 bases:

>100_bases
ACGCCTAAGTTCACGGCACTCACGTGCACCTATGTTTGCGCACTCGTCGTGTGATTTTTTTACATTCGGCCCTGCTGCTT
TAATCGGAGAGTCTCTGTAG

Downstream 100 bases:

>100_bases
AACCGTATCCGATCAAGTTCGTGTCGATCGTCATCCCTGTCTACAACGAGCGACAAAGCCTGCCAGAGTTGTTGCGCCGT
ACTGAAGCGGCCTGCGAACA

Product: UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrH; UDP-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate) aminotransferase; UDP-Ara4O aminotransferase; UDP-4-amino-4-deoxy-L-arabinose aminotransferase

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSQTFLAFSRPSIGDEEIAAVTRVLRSGWITTGPECQKLEEEFAARVGARHAVALSSATGAMHVALLALGVGPGDEVITP
SQTWVSTANMICLLGATPVFVDVDRDTLMTSAALIEHAITPRTKAIVPVHYAGAAFDLDPLYALADRHGITVIEDAAHAA
GTAYQGRPVGQQGTAIFSFHAIKNMTCAEGAMLVTDNARLADRVRQLKFHGLGVDAYDRLTLGRKPQAEVMEPGFKYNLA
DINASIARVQLQRLDAINAQRQALASHYLERLANSPVLPLALPRYAQQHAWHLFILRIDPERCGLDRDAFMKALQARNIG
TGIHFIATHLHSYYRKRFPDVRLPDTEWNSSRLCSIPLFPDMSLDDVERVVGAIESTLESSH

Sequences:

>Translated_382_residues
MSQTFLAFSRPSIGDEEIAAVTRVLRSGWITTGPECQKLEEEFAARVGARHAVALSSATGAMHVALLALGVGPGDEVITP
SQTWVSTANMICLLGATPVFVDVDRDTLMTSAALIEHAITPRTKAIVPVHYAGAAFDLDPLYALADRHGITVIEDAAHAA
GTAYQGRPVGQQGTAIFSFHAIKNMTCAEGAMLVTDNARLADRVRQLKFHGLGVDAYDRLTLGRKPQAEVMEPGFKYNLA
DINASIARVQLQRLDAINAQRQALASHYLERLANSPVLPLALPRYAQQHAWHLFILRIDPERCGLDRDAFMKALQARNIG
TGIHFIATHLHSYYRKRFPDVRLPDTEWNSSRLCSIPLFPDMSLDDVERVVGAIESTLESSH
>Mature_381_residues
SQTFLAFSRPSIGDEEIAAVTRVLRSGWITTGPECQKLEEEFAARVGARHAVALSSATGAMHVALLALGVGPGDEVITPS
QTWVSTANMICLLGATPVFVDVDRDTLMTSAALIEHAITPRTKAIVPVHYAGAAFDLDPLYALADRHGITVIEDAAHAAG
TAYQGRPVGQQGTAIFSFHAIKNMTCAEGAMLVTDNARLADRVRQLKFHGLGVDAYDRLTLGRKPQAEVMEPGFKYNLAD
INASIARVQLQRLDAINAQRQALASHYLERLANSPVLPLALPRYAQQHAWHLFILRIDPERCGLDRDAFMKALQARNIGT
GIHFIATHLHSYYRKRFPDVRLPDTEWNSSRLCSIPLFPDMSLDDVERVVGAIESTLESSH

Specific function: Catalyzes the conversion of UDP-4-keto-arabinose (UDP- Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family. ArnB subfamily

Homologues:

Organism=Escherichia coli, GI145693159, Length=371, Percent_Identity=62.8032345013477, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI2367285, Length=373, Percent_Identity=34.3163538873995, Blast_Score=168, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARNB_PSEU2 (Q4ZSZ4)

Other databases:

- EMBL:   CP000075
- RefSeq:   YP_235766.1
- ProteinModelPortal:   Q4ZSZ4
- SMR:   Q4ZSZ4
- STRING:   Q4ZSZ4
- GeneID:   3368206
- GenomeReviews:   CP000075_GR
- KEGG:   psb:Psyr_2689
- NMPDR:   fig|205918.4.peg.1765
- eggNOG:   COG0399
- HOGENOM:   HBG660897
- OMA:   SEWNSAR
- ProtClustDB:   PRK11658
- BioCyc:   PSYR205918:PSYR_2689-MONOMER
- HAMAP:   MF_01167
- InterPro:   IPR022850
- InterPro:   IPR000653
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF000390

Pfam domain/function: PF01041 DegT_DnrJ_EryC1; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.87

Molecular weight: Translated: 41810; Mature: 41679

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTFLAFSRPSIGDEEIAAVTRVLRSGWITTGPECQKLEEEFAARVGARHAVALSSATG
CCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHEEEEECCCH
AMHVALLALGVGPGDEVITPSQTWVSTANMICLLGATPVFVDVDRDTLMTSAALIEHAIT
HHHHHHHHHCCCCCCCCCCCCHHHHHHCCEEEEECCCEEEEECCHHHHHHHHHHHHHHCC
PRTKAIVPVHYAGAAFDLDPLYALADRHGITVIEDAAHAAGTAYQGRPVGQQGTAIFSFH
CCCCEEEEEEECCCEECCCHHHHHHCCCCCEEEECCHHHCCCCCCCCCCCCCCCEEEEEH
AIKNMTCAEGAMLVTDNARLADRVRQLKFHGLGVDAYDRLTLGRKPQAEVMEPGFKYNLA
HHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHCCCCEEEHH
DINASIARVQLQRLDAINAQRQALASHYLERLANSPVLPLALPRYAQQHAWHLFILRIDP
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEEEECH
ERCGLDRDAFMKALQARNIGTGIHFIATHLHSYYRKRFPDVRLPDTEWNSSRLCSIPLFP
HHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCC
DMSLDDVERVVGAIESTLESSH
CCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQTFLAFSRPSIGDEEIAAVTRVLRSGWITTGPECQKLEEEFAARVGARHAVALSSATG
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHEEEEECCCH
AMHVALLALGVGPGDEVITPSQTWVSTANMICLLGATPVFVDVDRDTLMTSAALIEHAIT
HHHHHHHHHCCCCCCCCCCCCHHHHHHCCEEEEECCCEEEEECCHHHHHHHHHHHHHHCC
PRTKAIVPVHYAGAAFDLDPLYALADRHGITVIEDAAHAAGTAYQGRPVGQQGTAIFSFH
CCCCEEEEEEECCCEECCCHHHHHHCCCCCEEEECCHHHCCCCCCCCCCCCCCCEEEEEH
AIKNMTCAEGAMLVTDNARLADRVRQLKFHGLGVDAYDRLTLGRKPQAEVMEPGFKYNLA
HHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHCCCCEEEHH
DINASIARVQLQRLDAINAQRQALASHYLERLANSPVLPLALPRYAQQHAWHLFILRIDP
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEEEECH
ERCGLDRDAFMKALQARNIGTGIHFIATHLHSYYRKRFPDVRLPDTEWNSSRLCSIPLFP
HHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCC
DMSLDDVERVVGAIESTLESSH
CCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA