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The map label for this gene is yhcA [H]

Identifier: 66045822

GI number: 66045822

Start: 3030855

End: 3032261

Strand: Direct

Name: yhcA [H]

Synonym: Psyr_2586

Alternate gene names: 66045822

Gene position: 3030855-3032261 (Clockwise)

Preceding gene: 66045821

Following gene: 66045823

Centisome position: 49.74

GC content: 65.17

Gene sequence:

>1407_bases
ATGAGTTTCAACGTCCCACGTAGCTGGGTAGTGGTCAACGTGCTGCTCGGTACGCTGACGGTCAGTCTGAGCAACAGTTC
GCTCAACCCGGCGCTGCCTGCCTTCATGGAGGCGTTCAGGATCGGGCCGCTGCTGGCCACCTGGATCGTCGCCGGATTCA
TGACCAGCATGGGCATGACCATGCCGCTGACCAGCTTCCTCAGTCAGCGCATCGGACGCAAACGCCTGTACCTGTGGGGT
GTGGCGCTGTTCATTGGCGGCTCGCTGCTGGGCGCGCTGGCCGACTCCATCGCGCTGGTGATCACCGCACGGGTGGTGCA
GGGCATTGCCAGCGGGCTGATGATCCCCTTGTCGCTGGCGATCATCTTTTCGGTGTATGAAAAGCACGAACGGGGCAGGG
TGACCGGGCTCTGGAGCGCAGCAGTGATGCTCGCACCGGCGCTCGGTCCGCTGTGCGGCAGCCTGATGCTGGAGTGGTTC
AGCTGGCGCTCGCTGTTCCTGATGAATGTGCCCATCGGCCTGCTGGCGCTGTTGCTGGGCGTCGGCGTGCTGCCGGACGC
CGAGCCCGCCGAGCGCAAGCCGTTCGATTTGATCGGCTACCTGCTGGTGGCCTCGGGCATCGGCTTGCTGATGATCGCCA
TTAGCCGCATGCACCATGCTCAGGCGCTGCTCGATCCAGTCAATCAGGCCATGGTGCTGGTCGCATTGGCCTGTCTGGTG
GCATTCGTCCGGGTCGAGCTGAGTCGCCAGGCTCCGCTGCTGAATCTGCGTCTGTTCAACCTGCGCGGTTATCGTCTGAG
CGTGATCATCGCCGTGGTGCAATCGGTCGGCATGTTCGAGTGCCTGGTGTTGCTGCCGCTGCTGGTGCAGAACGTGCTGG
GCTACAACCCGATCTGGACCGGCCTGGCGCTGCTGTGTACCGCCAGCTTCGCGAGCCTGTTCGGGCAGTGGGGCGGCAAG
GCGCTGGATCGCCATGGCCCACGCACCGTCGTCGCCATCGGCCTGCTGCTGACCGGCGTTTCGACCCTGGCGCTGGGCAT
GCTCAAGGCCGACGCCGCGATCGGCGTGGTGTTCGTCCTGATGATGATTCGTGGCGCCGGCCTCGGGCTCTCCTATATGC
CGGTGACTACGGCGGGGCTCAATGCCTTGCCCGAGCCGATGGTCACTCAGGGCGCCGCGATGAACAACATTTCGCGCCGT
CTGGTCGCGTCGCTGGCCATCGTCATCGCCTCGCTGTGGCTGGAGTTCCGCCTCAACACCGCGGGCCCGGCCGCCACGCC
CTCGGCCATCAGCGAAGTATTCATCGCCACCGGCGTGCTGATCCTGCTGGCACTGCCGTGTGCCTGGCGTTTTCCCCTTA
ACGACGAGCGCGCCGAGGCCCAGCCCGACGCGGTCGAACCCCGATAA

Upstream 100 bases:

>100_bases
CGCTGGCGGGAGCGTATGGCTACAACATTTCCCATGCCGACTTTCTCTGTCATCCACGACCGTCACAGCACTTCGTGCGC
AACGGCGAACGGGTTCGCCA

Downstream 100 bases:

>100_bases
TTTTTCGTTCGAGGTATGAACATGGCTACCCCTTTACCGCTGCAACGTAACGCCGCTTCGACAACGACAACAGCTGGTAC
TGGTGCCTGGCTGGCCGACG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSFNVPRSWVVVNVLLGTLTVSLSNSSLNPALPAFMEAFRIGPLLATWIVAGFMTSMGMTMPLTSFLSQRIGRKRLYLWG
VALFIGGSLLGALADSIALVITARVVQGIASGLMIPLSLAIIFSVYEKHERGRVTGLWSAAVMLAPALGPLCGSLMLEWF
SWRSLFLMNVPIGLLALLLGVGVLPDAEPAERKPFDLIGYLLVASGIGLLMIAISRMHHAQALLDPVNQAMVLVALACLV
AFVRVELSRQAPLLNLRLFNLRGYRLSVIIAVVQSVGMFECLVLLPLLVQNVLGYNPIWTGLALLCTASFASLFGQWGGK
ALDRHGPRTVVAIGLLLTGVSTLALGMLKADAAIGVVFVLMMIRGAGLGLSYMPVTTAGLNALPEPMVTQGAAMNNISRR
LVASLAIVIASLWLEFRLNTAGPAATPSAISEVFIATGVLILLALPCAWRFPLNDERAEAQPDAVEPR

Sequences:

>Translated_468_residues
MSFNVPRSWVVVNVLLGTLTVSLSNSSLNPALPAFMEAFRIGPLLATWIVAGFMTSMGMTMPLTSFLSQRIGRKRLYLWG
VALFIGGSLLGALADSIALVITARVVQGIASGLMIPLSLAIIFSVYEKHERGRVTGLWSAAVMLAPALGPLCGSLMLEWF
SWRSLFLMNVPIGLLALLLGVGVLPDAEPAERKPFDLIGYLLVASGIGLLMIAISRMHHAQALLDPVNQAMVLVALACLV
AFVRVELSRQAPLLNLRLFNLRGYRLSVIIAVVQSVGMFECLVLLPLLVQNVLGYNPIWTGLALLCTASFASLFGQWGGK
ALDRHGPRTVVAIGLLLTGVSTLALGMLKADAAIGVVFVLMMIRGAGLGLSYMPVTTAGLNALPEPMVTQGAAMNNISRR
LVASLAIVIASLWLEFRLNTAGPAATPSAISEVFIATGVLILLALPCAWRFPLNDERAEAQPDAVEPR
>Mature_467_residues
SFNVPRSWVVVNVLLGTLTVSLSNSSLNPALPAFMEAFRIGPLLATWIVAGFMTSMGMTMPLTSFLSQRIGRKRLYLWGV
ALFIGGSLLGALADSIALVITARVVQGIASGLMIPLSLAIIFSVYEKHERGRVTGLWSAAVMLAPALGPLCGSLMLEWFS
WRSLFLMNVPIGLLALLLGVGVLPDAEPAERKPFDLIGYLLVASGIGLLMIAISRMHHAQALLDPVNQAMVLVALACLVA
FVRVELSRQAPLLNLRLFNLRGYRLSVIIAVVQSVGMFECLVLLPLLVQNVLGYNPIWTGLALLCTASFASLFGQWGGKA
LDRHGPRTVVAIGLLLTGVSTLALGMLKADAAIGVVFVLMMIRGAGLGLSYMPVTTAGLNALPEPMVTQGAAMNNISRRL
VASLAIVIASLWLEFRLNTAGPAATPSAISEVFIATGVLILLALPCAWRFPLNDERAEAQPDAVEPR

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=417, Percent_Identity=28.2973621103118, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789042, Length=405, Percent_Identity=27.4074074074074, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=28.9855072463768, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790195, Length=420, Percent_Identity=24.047619047619, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788392, Length=422, Percent_Identity=24.1706161137441, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790146, Length=144, Percent_Identity=28.4722222222222, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=145, Percent_Identity=29.6551724137931, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49916; Mature: 49784

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFNVPRSWVVVNVLLGTLTVSLSNSSLNPALPAFMEAFRIGPLLATWIVAGFMTSMGMT
CCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH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CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]