Definition Pseudomonas syringae pv. syringae B728a, complete genome.
Accession NC_007005
Length 6,093,698

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The map label for this gene is ygfF [H]

Identifier: 66045723

GI number: 66045723

Start: 2890843

End: 2891589

Strand: Direct

Name: ygfF [H]

Synonym: Psyr_2487

Alternate gene names: 66045723

Gene position: 2890843-2891589 (Clockwise)

Preceding gene: 66045722

Following gene: 66045725

Centisome position: 47.44

GC content: 60.78

Gene sequence:

>747_bases
ATGGAAAAAGTCCTCATTATCACCGGTGGTTCGCGCGGGATCGGCGCTGCTACAGCGCGTCTGGCCGCTTCACAGGGTTA
TCGGATCTGCATCAACTACCTGTCGGATCATGCCGCTGCCGAAAAGACCGCAGGCCAGGTGAGGGCAATGGGCGCCAGGG
CGATCACCGTGCAGGCCGATGTCAGCAACGAAGACGAGATCATGCGGCTGTTTGCCAGGGTCGACTCCGAACTCGGCCGG
GTTACCCATCTGGTCAATAATGCGGGCACCCTGGCGCAGGCTTCCAGAGTCGAGGACATGTCGGAATTTCGCCTGTTGAA
GATGATGATGAACAACGTCGTCGGCCCGATGCTGTGCAGCAAACATGCCTTGTTGCGCATGTTGCCGGCACACGGGGGGC
AAGGCGGCAGCATCGTCAACGTATCGTCACTCGCGGCACGCCTGGGCTCGGCAGGCGAGTACGTCGACTATGCAGCATCC
AAAGGAGCGCTGGACACCTTCACCATCGGGCTCTCCAAAGAGGTCGCGGCGGAAAACGTTCGCGTCAACGCGGTACGTCC
GGGGTTCATCTTCACCGACTTTCACGCCCTGAGCGGCGATCCGTTCCGCGTCAGCAAGCTTGAGGGGGCATTGCCCATGG
GCCGTGGCGGTACCGCCGAGGAAGTGGCCGAAGCGATTCTCTGGTTGCTGTCGGACAATGCGTCTTATGCCACGGGAACA
TTCATCGATGTGGCGGGAGGGCGCTAA

Upstream 100 bases:

>100_bases
GGCGCTGGACGATAAAGGCTGTCACGATCCGAACCGGCTGGTTAACCTGCGAAGGTTGGCTGGATCCTGATGTAGGCCTT
TGAAAAAAACGAGACACTCC

Downstream 100 bases:

>100_bases
AGGCGTCCATACTCGGGCAGACGCCTTCAGATCCTGCTTACTGGTCACACCGTGACGGGCGGCGCATTGGCTGGCGGATC
GCCGACTGGCGTTTCATTGG

Product: Short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MEKVLIITGGSRGIGAATARLAASQGYRICINYLSDHAAAEKTAGQVRAMGARAITVQADVSNEDEIMRLFARVDSELGR
VTHLVNNAGTLAQASRVEDMSEFRLLKMMMNNVVGPMLCSKHALLRMLPAHGGQGGSIVNVSSLAARLGSAGEYVDYAAS
KGALDTFTIGLSKEVAAENVRVNAVRPGFIFTDFHALSGDPFRVSKLEGALPMGRGGTAEEVAEAILWLLSDNASYATGT
FIDVAGGR

Sequences:

>Translated_248_residues
MEKVLIITGGSRGIGAATARLAASQGYRICINYLSDHAAAEKTAGQVRAMGARAITVQADVSNEDEIMRLFARVDSELGR
VTHLVNNAGTLAQASRVEDMSEFRLLKMMMNNVVGPMLCSKHALLRMLPAHGGQGGSIVNVSSLAARLGSAGEYVDYAAS
KGALDTFTIGLSKEVAAENVRVNAVRPGFIFTDFHALSGDPFRVSKLEGALPMGRGGTAEEVAEAILWLLSDNASYATGT
FIDVAGGR
>Mature_248_residues
MEKVLIITGGSRGIGAATARLAASQGYRICINYLSDHAAAEKTAGQVRAMGARAITVQADVSNEDEIMRLFARVDSELGR
VTHLVNNAGTLAQASRVEDMSEFRLLKMMMNNVVGPMLCSKHALLRMLPAHGGQGGSIVNVSSLAARLGSAGEYVDYAAS
KGALDTFTIGLSKEVAAENVRVNAVRPGFIFTDFHALSGDPFRVSKLEGALPMGRGGTAEEVAEAILWLLSDNASYATGT
FIDVAGGR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=251, Percent_Identity=38.2470119521912, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI15277342, Length=249, Percent_Identity=30.1204819277108, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI59889578, Length=200, Percent_Identity=34, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI19923817, Length=253, Percent_Identity=29.6442687747036, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI32483357, Length=246, Percent_Identity=31.3008130081301, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=28.6290322580645, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI5031737, Length=245, Percent_Identity=29.7959183673469, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI7705925, Length=252, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI10190704, Length=256, Percent_Identity=31.25, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI31542939, Length=193, Percent_Identity=30.5699481865285, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI210032110, Length=196, Percent_Identity=30.6122448979592, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI4758504, Length=213, Percent_Identity=28.169014084507, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI2367175, Length=245, Percent_Identity=51.8367346938776, Blast_Score=264, Evalue=5e-72,
Organism=Escherichia coli, GI1788459, Length=247, Percent_Identity=35.2226720647773, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787335, Length=245, Percent_Identity=31.4285714285714, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789378, Length=245, Percent_Identity=29.3877551020408, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=31.3253012048193, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082160, Length=248, Percent_Identity=28.6290322580645, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87082100, Length=252, Percent_Identity=31.3492063492063, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790717, Length=246, Percent_Identity=27.6422764227642, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367365, Length=247, Percent_Identity=31.5789473684211, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787891, Length=248, Percent_Identity=30.6451612903226, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787905, Length=245, Percent_Identity=29.3877551020408, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1789057, Length=260, Percent_Identity=27.3076923076923, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786812, Length=257, Percent_Identity=27.6264591439689, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=245, Percent_Identity=36.3265306122449, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25147288, Length=245, Percent_Identity=34.2857142857143, Blast_Score=113, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71994600, Length=258, Percent_Identity=37.5968992248062, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI115534694, Length=247, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71994604, Length=256, Percent_Identity=34.375, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559104, Length=268, Percent_Identity=30.2238805970149, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17538486, Length=263, Percent_Identity=29.6577946768061, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17544670, Length=263, Percent_Identity=30.7984790874525, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=251, Percent_Identity=29.4820717131474, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=267, Percent_Identity=29.2134831460674, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=31.0606060606061, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17560220, Length=266, Percent_Identity=33.0827067669173, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17562910, Length=266, Percent_Identity=31.9548872180451, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17562990, Length=265, Percent_Identity=31.3207547169811, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17562906, Length=264, Percent_Identity=30.3030303030303, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17551412, Length=264, Percent_Identity=29.1666666666667, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17563726, Length=263, Percent_Identity=29.6577946768061, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17538182, Length=213, Percent_Identity=30.5164319248826, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=271, Percent_Identity=29.520295202952, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560332, Length=264, Percent_Identity=31.4393939393939, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=28.3018867924528, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=270, Percent_Identity=30.3703703703704, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17560676, Length=250, Percent_Identity=29.2, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=266, Percent_Identity=30.0751879699248, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17536025, Length=256, Percent_Identity=28.90625, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193203117, Length=192, Percent_Identity=31.25, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=192, Percent_Identity=31.25, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=29.9242424242424, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=26.7716535433071, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=253, Percent_Identity=27.6679841897233, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=244, Percent_Identity=29.5081967213115, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=194, Percent_Identity=27.8350515463918, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=198, Percent_Identity=30.8080808080808, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI23397609, Length=254, Percent_Identity=37.007874015748, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=37.109375, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=36.7588932806324, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=33.4661354581673, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24639444, Length=246, Percent_Identity=30.8943089430894, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355319, Length=247, Percent_Identity=32.3886639676113, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24643142, Length=249, Percent_Identity=30.1204819277108, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24584748, Length=196, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161076933, Length=196, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644337, Length=120, Percent_Identity=41.6666666666667, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648693, Length=242, Percent_Identity=26.8595041322314, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21355889, Length=242, Percent_Identity=26.8595041322314, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007236, Length=244, Percent_Identity=27.4590163934426, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737361, Length=203, Percent_Identity=27.0935960591133, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281364494, Length=201, Percent_Identity=27.363184079602, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26118; Mature: 26118

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MEKVLIITGGSRGIGAATARLAASQGYRICINYLSDHAAAEKTAGQVRAMGARAITVQAD
CCEEEEEECCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
VSNEDEIMRLFARVDSELGRVTHLVNNAGTLAQASRVEDMSEFRLLKMMMNNVVGPMLCS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHALLRMLPAHGGQGGSIVNVSSLAARLGSAGEYVDYAASKGALDTFTIGLSKEVAAENV
HHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCHHHHHCCCCCCEEEEECCCHHHHHCCE
RVNAVRPGFIFTDFHALSGDPFRVSKLEGALPMGRGGTAEEVAEAILWLLSDNASYATGT
EEEEECCCEEEEEHHHCCCCCEEEEHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
FIDVAGGR
EEECCCCC
>Mature Secondary Structure
MEKVLIITGGSRGIGAATARLAASQGYRICINYLSDHAAAEKTAGQVRAMGARAITVQAD
CCEEEEEECCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
VSNEDEIMRLFARVDSELGRVTHLVNNAGTLAQASRVEDMSEFRLLKMMMNNVVGPMLCS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHALLRMLPAHGGQGGSIVNVSSLAARLGSAGEYVDYAASKGALDTFTIGLSKEVAAENV
HHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCHHHHHCCCCCCEEEEECCCHHHHHCCE
RVNAVRPGFIFTDFHALSGDPFRVSKLEGALPMGRGGTAEEVAEAILWLLSDNASYATGT
EEEEECCCEEEEEHHHCCCCCEEEEHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
FIDVAGGR
EEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 7764507 [H]