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The map label for this gene is pbuE [H]

Identifier: 66045657

GI number: 66045657

Start: 2803152

End: 2804306

Strand: Direct

Name: pbuE [H]

Synonym: Psyr_2421

Alternate gene names: 66045657

Gene position: 2803152-2804306 (Clockwise)

Preceding gene: 66045656

Following gene: 66045659

Centisome position: 46.0

GC content: 62.42

Gene sequence:

>1155_bases
ATGTTGTTGCCCATCCTTCTGCTGTCAGCCGCCGGGTTTACCGTGCTGACCACAGAATTCGTCATCGTCGGTCTTTTGCC
AGCGGTTGCCCGCGACCTGAATGTCACGGTCTCCCAGGCAGGTCTGCTGGTGACGCTGTTTGCGTTTACCGTCGCCGCCT
TTGGTCCTTTTCTGACCGCCTATTTTTCGCGCTTCGAGCGCAAGCGCTTGTTTGTCAGCATCCTGGTGCTGTTCGGCTTT
GCAAATGTCCTGGCGGCGCTGGCGCCCAATATCGCCGTGATGGGGATCGCCCGGCTGATTCCTGCGTTGGGGTTACCGGT
GTTCTGGGCGCTGGCCAGCGAGACCGCCGTGGACATTGTCGGTCCGCAGTTCGCAGGCCGGGCAATTGCCAGGATCGGTT
TCGGCATCGTTTGCGCCACGGTATTCGGCATTCCCATCGGCACCCTGATCTCCGATGCCTTCGGCTGGCGCACGGCCTTT
CTGGTTCTGGCCGCTCTGGCGTTCGCCAAGGCACTGTTGCTGACGGTCTATCTGCCGAAGACGGCTGCCAGGCAGAACCC
GGTATCGATCCTTAAGCAGTTCGGCATCTTGCGCAGCCGGATGATGCAGGGGCACGTGCTGCTCTCGGTGCTGGTGTTCA
GCGGCATGTTCACGGCCTATACCTATCTGGCGGACATGCTCGAGCGTCTTGCCGGTTTCGACGGCGCTCTGGTGGGCTGG
TGCCTGATGGGCTTTGGCGCTGTCGGCTTGCTGGGCAACTCGCTGGGCGGGCGCATGGTCGACCGCCATCCGTTGATTGC
CAGCCTGGTGTTCTGCGCATTCATGGTGGTTGGCATGGTCGCGGTGGTGCCGAGCATCCATTCGGTGGTGGCCTTGTCAC
TCGCGCTGGCGGTCTGGGGTATCACCCAGGCGGCCTTGTTTCTGGTCAGTCATGTGCGGTTGATCAAGGCCGCGCCGCAA
GCGCCTGCGTTCGCGGCGTCACTGAACATCGCTGGTGCCAACCTCGGCATTGGCATCGGCGCGGTGATCGGCGGCAGGGT
CATTGATCACCTGGGGCTGGGCAACGTCGGTTTTGCCGCTGCGGGCATCATTGTGCTGGCGATTGTGCTGGCGCTGCTGT
TGATCAAGCGTGATCCGGGGCCGCTCCCGGCTTAG

Upstream 100 bases:

>100_bases
AGTGCGAAACATTGGCGTGACAGTCATCTTCAAGCTACTTTAATCCCCCTCCCGCAGGCCGCGCTGTTGCAGCGGGCTCT
GTTGTTGTTCTGGAGTATTC

Downstream 100 bases:

>100_bases
CCAGCATCGGTAAACAGCTGATTCCGAGCACTTTCGGCAATGGCGACGACGCCGGGGTGCTTGACCTTGCGCTCCACGGT
GATGGCGTAGAACGATTCGG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MLLPILLLSAAGFTVLTTEFVIVGLLPAVARDLNVTVSQAGLLVTLFAFTVAAFGPFLTAYFSRFERKRLFVSILVLFGF
ANVLAALAPNIAVMGIARLIPALGLPVFWALASETAVDIVGPQFAGRAIARIGFGIVCATVFGIPIGTLISDAFGWRTAF
LVLAALAFAKALLLTVYLPKTAARQNPVSILKQFGILRSRMMQGHVLLSVLVFSGMFTAYTYLADMLERLAGFDGALVGW
CLMGFGAVGLLGNSLGGRMVDRHPLIASLVFCAFMVVGMVAVVPSIHSVVALSLALAVWGITQAALFLVSHVRLIKAAPQ
APAFAASLNIAGANLGIGIGAVIGGRVIDHLGLGNVGFAAAGIIVLAIVLALLLIKRDPGPLPA

Sequences:

>Translated_384_residues
MLLPILLLSAAGFTVLTTEFVIVGLLPAVARDLNVTVSQAGLLVTLFAFTVAAFGPFLTAYFSRFERKRLFVSILVLFGF
ANVLAALAPNIAVMGIARLIPALGLPVFWALASETAVDIVGPQFAGRAIARIGFGIVCATVFGIPIGTLISDAFGWRTAF
LVLAALAFAKALLLTVYLPKTAARQNPVSILKQFGILRSRMMQGHVLLSVLVFSGMFTAYTYLADMLERLAGFDGALVGW
CLMGFGAVGLLGNSLGGRMVDRHPLIASLVFCAFMVVGMVAVVPSIHSVVALSLALAVWGITQAALFLVSHVRLIKAAPQ
APAFAASLNIAGANLGIGIGAVIGGRVIDHLGLGNVGFAAAGIIVLAIVLALLLIKRDPGPLPA
>Mature_384_residues
MLLPILLLSAAGFTVLTTEFVIVGLLPAVARDLNVTVSQAGLLVTLFAFTVAAFGPFLTAYFSRFERKRLFVSILVLFGF
ANVLAALAPNIAVMGIARLIPALGLPVFWALASETAVDIVGPQFAGRAIARIGFGIVCATVFGIPIGTLISDAFGWRTAF
LVLAALAFAKALLLTVYLPKTAARQNPVSILKQFGILRSRMMQGHVLLSVLVFSGMFTAYTYLADMLERLAGFDGALVGW
CLMGFGAVGLLGNSLGGRMVDRHPLIASLVFCAFMVVGMVAVVPSIHSVVALSLALAVWGITQAALFLVSHVRLIKAAPQ
APAFAASLNIAGANLGIGIGAVIGGRVIDHLGLGNVGFAAAGIIVLAIVLALLLIKRDPGPLPA

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine and 6-mercaptopurine. Therefore plays a role in maintaining t

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=327, Percent_Identity=29.0519877675841, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1787808, Length=379, Percent_Identity=27.7044854881267, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI145693198, Length=350, Percent_Identity=26, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786595, Length=380, Percent_Identity=26.0526315789474, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 39989; Mature: 39989

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPILLLSAAGFTVLTTEFVIVGLLPAVARDLNVTVSQAGLLVTLFAFTVAAFGPFLTA
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YFSRFERKRLFVSILVLFGFANVLAALAPNIAVMGIARLIPALGLPVFWALASETAVDIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHHC
GPQFAGRAIARIGFGIVCATVFGIPIGTLISDAFGWRTAFLVLAALAFAKALLLTVYLPK
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
TAARQNPVSILKQFGILRSRMMQGHVLLSVLVFSGMFTAYTYLADMLERLAGFDGALVGW
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
CLMGFGAVGLLGNSLGGRMVDRHPLIASLVFCAFMVVGMVAVVPSIHSVVALSLALAVWG
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITQAALFLVSHVRLIKAAPQAPAFAASLNIAGANLGIGIGAVIGGRVIDHLGLGNVGFAA
HHHHHHHHHHHHHHHHCCCCCCCHHHHCEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHH
AGIIVLAIVLALLLIKRDPGPLPA
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLLPILLLSAAGFTVLTTEFVIVGLLPAVARDLNVTVSQAGLLVTLFAFTVAAFGPFLTA
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YFSRFERKRLFVSILVLFGFANVLAALAPNIAVMGIARLIPALGLPVFWALASETAVDIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHHC
GPQFAGRAIARIGFGIVCATVFGIPIGTLISDAFGWRTAFLVLAALAFAKALLLTVYLPK
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
TAARQNPVSILKQFGILRSRMMQGHVLLSVLVFSGMFTAYTYLADMLERLAGFDGALVGW
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
CLMGFGAVGLLGNSLGGRMVDRHPLIASLVFCAFMVVGMVAVVPSIHSVVALSLALAVWG
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITQAALFLVSHVRLIKAAPQAPAFAASLNIAGANLGIGIGAVIGGRVIDHLGLGNVGFAA
HHHHHHHHHHHHHHHHCCCCCCCHHHHCEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHH
AGIIVLAIVLALLLIKRDPGPLPA
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA