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The map label for this gene is poxB [H]

Identifier: 66045554

GI number: 66045554

Start: 2688510

End: 2690315

Strand: Direct

Name: poxB [H]

Synonym: Psyr_2314

Alternate gene names: 66045554

Gene position: 2688510-2690315 (Clockwise)

Preceding gene: 66045553

Following gene: 66045555

Centisome position: 44.12

GC content: 61.07

Gene sequence:

>1806_bases
ATGCATTGGGTCGACCACAGCAGGCGTGGCAGAGCGCTGCGCGCGTTTTACCGAACTCATTATCATTGCCTCGACAAGGA
ACCCCGCATGTCCAAGACCATTGCCGACCACCTCGCGCAAACCCTCGCAGCGGCAGGTGTTTCGCACATCTGGGGGGTGT
CTGGTGACAGCCTCAACGGCCTGACCGACAGCCTGGAACGCACCGATTCGATTCGCTGGATGCACACCCGTCATGAAGAA
GTCGCAGCCTTTGCTGCCGGTGCCCAGGCAGCGTCCAGCGGCAAGCTGGCGGTCTGCGCCGGCAGCTGCGGGCCGGGCAA
CCTGCACCTGATCAACGGCCTGTATGACTGCCACCGCAACCGTGTACCGGTACTGGCAATTGCTGCGCATATTCCGTCCT
CGGAAATCGGTCTGGATTACTTTCAGGAAACCCATCCGCAAGAGCTGTTCAAGGAGTGCAGCCACTTCGTCGAGCTGGTC
AGCAACCCCGAGCAGTTCCCGCGTGTGCTGGAACGTGCCATGCGTGCCGCGATCAGCCAGAAAGGTGTGGCGGTGATTGT
TTTGCCGGGCGACGTGGCGCTCAGCGACGCGCCGGATGTCGCGGCAAAATGGATCGAGGCTACAGCGCCCAAGGTCGTGC
CTGCCGAAAACGACCTGCAGTCGATGGCCGACATGCTCAACGCTTCCAAAGCCGTCACGCTGCTGTGCGGTGCGGGTTGC
GAGGGTGCCCACGAGCAGCTTCTGGCATTGGCGGATACGCTGGGCGCGCCGATCGTTCACGCATTGCGCGGCAAGCAGCA
CGTTGAGTACGACAACCCGTTCGACGTCGGCATGACCGGCCTGATCGGGTTCAGCTCCGGCTACCACGCCATGTTGTCCT
GCGACACACTGGTGATTCTGGGCAGCAGCTTTCCGTACCGGAATTTCTACCCTGAAAAAGCCGATATCATCCAGATAGAC
CTCGATCCGACTCAGATCGGTCGCCGTACGCCGGTGGCGCTGGGTCTGGTTGGTGGGGTGCGGGACACCCTTGAAGCGCT
TCAGCCAAAACTGAAGCCGCATACCGATCGCCGTTTCCTCGACAAGGCCCTCAAGCATTACGCCAAGGCCCGGGAAGAAC
TGGACGAGCTGGCCACTCCGACGCCAGATGGCGCGCCGATTCACCCGCAGTACCTCACTCGACTGGTCGACGAGCAGGCG
GATGCCGATGCGATCTTTACCGTCGACGTCGGTACGCCGACACTGTGGGCTGCGCGCTATCTGCACATGAACGGCAAGCG
CAGTCTGCTCGGCTCGTTCAACCACGGCTCGATGGCCAATGCCTTGCCACAGGCTTTAGGGGCCAAGGCTGAACATCCGG
ATCGTCAGGTCATCGCCCTGTGTGGCGACGGCGGCCTGTCGATGTTGCTGGGCGACCTCCTGAGCATTCGTCAGCTGAAC
CTGCCTATCAAGATGGTGGTGTTCAACAACAGCTCGCTGGGCTTTGTCGACATGGAAATGAAAGCCAGTGGTTATGTGCC
GCACGGCACTGATCTGCATGAAACCAACTTCGCCGGTATTGCCCTGGGCGCAGGCATTCTGGGCCTGCGTGTGGAGAACG
CCGAAGAGCTGCCCGCCGCCTTGCGCAAGGCGTTCGATCATCCCGGTCCAGTGCTGATCGACGTTGTCACCGCCAAGCAG
GAGCTGGGCATTCCACCGAAAATCAAGCTGGCACAGGCCAGGGGCTTCAGTCTGTACATGATGCGCGCGATCATGAGCGG
ACGCGGCAGTGAAGTGCTGGAACTGGCGAAAACCAACCTGCGTTAA

Upstream 100 bases:

>100_bases
CGCCAAGGCACCGGGCAACCCGCACGCCTGATCAGCGTTTTAAAGCGCTGGCCGAATGACCGAGCCCTGAACAGGGCTCA
GGTTTTCTGAACTGTCGAGA

Downstream 100 bases:

>100_bases
TACATTATAAAAACGTATGGGATGAATAGAAAAAGTTGCAACTTCTATCCACCGAGATTGTCGTCGCTGTTACAGGTGAT
ACGCTCGGTGTCTTTATATT

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MHWVDHSRRGRALRAFYRTHYHCLDKEPRMSKTIADHLAQTLAAAGVSHIWGVSGDSLNGLTDSLERTDSIRWMHTRHEE
VAAFAAGAQAASSGKLAVCAGSCGPGNLHLINGLYDCHRNRVPVLAIAAHIPSSEIGLDYFQETHPQELFKECSHFVELV
SNPEQFPRVLERAMRAAISQKGVAVIVLPGDVALSDAPDVAAKWIEATAPKVVPAENDLQSMADMLNASKAVTLLCGAGC
EGAHEQLLALADTLGAPIVHALRGKQHVEYDNPFDVGMTGLIGFSSGYHAMLSCDTLVILGSSFPYRNFYPEKADIIQID
LDPTQIGRRTPVALGLVGGVRDTLEALQPKLKPHTDRRFLDKALKHYAKAREELDELATPTPDGAPIHPQYLTRLVDEQA
DADAIFTVDVGTPTLWAARYLHMNGKRSLLGSFNHGSMANALPQALGAKAEHPDRQVIALCGDGGLSMLLGDLLSIRQLN
LPIKMVVFNNSSLGFVDMEMKASGYVPHGTDLHETNFAGIALGAGILGLRVENAEELPAALRKAFDHPGPVLIDVVTAKQ
ELGIPPKIKLAQARGFSLYMMRAIMSGRGSEVLELAKTNLR

Sequences:

>Translated_601_residues
MHWVDHSRRGRALRAFYRTHYHCLDKEPRMSKTIADHLAQTLAAAGVSHIWGVSGDSLNGLTDSLERTDSIRWMHTRHEE
VAAFAAGAQAASSGKLAVCAGSCGPGNLHLINGLYDCHRNRVPVLAIAAHIPSSEIGLDYFQETHPQELFKECSHFVELV
SNPEQFPRVLERAMRAAISQKGVAVIVLPGDVALSDAPDVAAKWIEATAPKVVPAENDLQSMADMLNASKAVTLLCGAGC
EGAHEQLLALADTLGAPIVHALRGKQHVEYDNPFDVGMTGLIGFSSGYHAMLSCDTLVILGSSFPYRNFYPEKADIIQID
LDPTQIGRRTPVALGLVGGVRDTLEALQPKLKPHTDRRFLDKALKHYAKAREELDELATPTPDGAPIHPQYLTRLVDEQA
DADAIFTVDVGTPTLWAARYLHMNGKRSLLGSFNHGSMANALPQALGAKAEHPDRQVIALCGDGGLSMLLGDLLSIRQLN
LPIKMVVFNNSSLGFVDMEMKASGYVPHGTDLHETNFAGIALGAGILGLRVENAEELPAALRKAFDHPGPVLIDVVTAKQ
ELGIPPKIKLAQARGFSLYMMRAIMSGRGSEVLELAKTNLR
>Mature_601_residues
MHWVDHSRRGRALRAFYRTHYHCLDKEPRMSKTIADHLAQTLAAAGVSHIWGVSGDSLNGLTDSLERTDSIRWMHTRHEE
VAAFAAGAQAASSGKLAVCAGSCGPGNLHLINGLYDCHRNRVPVLAIAAHIPSSEIGLDYFQETHPQELFKECSHFVELV
SNPEQFPRVLERAMRAAISQKGVAVIVLPGDVALSDAPDVAAKWIEATAPKVVPAENDLQSMADMLNASKAVTLLCGAGC
EGAHEQLLALADTLGAPIVHALRGKQHVEYDNPFDVGMTGLIGFSSGYHAMLSCDTLVILGSSFPYRNFYPEKADIIQID
LDPTQIGRRTPVALGLVGGVRDTLEALQPKLKPHTDRRFLDKALKHYAKAREELDELATPTPDGAPIHPQYLTRLVDEQA
DADAIFTVDVGTPTLWAARYLHMNGKRSLLGSFNHGSMANALPQALGAKAEHPDRQVIALCGDGGLSMLLGDLLSIRQLN
LPIKMVVFNNSSLGFVDMEMKASGYVPHGTDLHETNFAGIALGAGILGLRVENAEELPAALRKAFDHPGPVLIDVVTAKQ
ELGIPPKIKLAQARGFSLYMMRAIMSGRGSEVLELAKTNLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=24.6869409660107, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=65.4991243432574, Blast_Score=760, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=28.673835125448, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87081685, Length=555, Percent_Identity=26.1261261261261, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1786717, Length=552, Percent_Identity=25.3623188405797, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788716, Length=543, Percent_Identity=23.0202578268877, Blast_Score=90, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17531299, Length=567, Percent_Identity=24.1622574955908, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=567, Percent_Identity=24.1622574955908, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=557, Percent_Identity=25.8527827648115, Blast_Score=168, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6320816, Length=537, Percent_Identity=22.3463687150838, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922626, Length=476, Percent_Identity=22.4789915966387, Blast_Score=92, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 65043; Mature: 65043

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHWVDHSRRGRALRAFYRTHYHCLDKEPRMSKTIADHLAQTLAAAGVSHIWGVSGDSLNG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LTDSLERTDSIRWMHTRHEEVAAFAAGAQAASSGKLAVCAGSCGPGNLHLINGLYDCHRN
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEHHHHHHCCC
RVPVLAIAAHIPSSEIGLDYFQETHPQELFKECSHFVELVSNPEQFPRVLERAMRAAISQ
CCCEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
KGVAVIVLPGDVALSDAPDVAAKWIEATAPKVVPAENDLQSMADMLNASKAVTLLCGAGC
CCCEEEEECCCEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCC
EGAHEQLLALADTLGAPIVHALRGKQHVEYDNPFDVGMTGLIGFSSGYHAMLSCDTLVIL
CHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCEEEEECCEEEEE
GSSFPYRNFYPEKADIIQIDLDPTQIGRRTPVALGLVGGVRDTLEALQPKLKPHTDRRFL
CCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHH
DKALKHYAKAREELDELATPTPDGAPIHPQYLTRLVDEQADADAIFTVDVGTPTLWAARY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
LHMNGKRSLLGSFNHGSMANALPQALGAKAEHPDRQVIALCGDGGLSMLLGDLLSIRQLN
HHCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
LPIKMVVFNNSSLGFVDMEMKASGYVPHGTDLHETNFAGIALGAGILGLRVENAEELPAA
CCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCEEEEEECCHHHHHHH
LRKAFDHPGPVLIDVVTAKQELGIPPKIKLAQARGFSLYMMRAIMSGRGSEVLELAKTNL
HHHHHCCCCCEEEEEEECHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCC
R
C
>Mature Secondary Structure
MHWVDHSRRGRALRAFYRTHYHCLDKEPRMSKTIADHLAQTLAAAGVSHIWGVSGDSLNG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LTDSLERTDSIRWMHTRHEEVAAFAAGAQAASSGKLAVCAGSCGPGNLHLINGLYDCHRN
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEHHHHHHCCC
RVPVLAIAAHIPSSEIGLDYFQETHPQELFKECSHFVELVSNPEQFPRVLERAMRAAISQ
CCCEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
KGVAVIVLPGDVALSDAPDVAAKWIEATAPKVVPAENDLQSMADMLNASKAVTLLCGAGC
CCCEEEEECCCEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCC
EGAHEQLLALADTLGAPIVHALRGKQHVEYDNPFDVGMTGLIGFSSGYHAMLSCDTLVIL
CHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCEEEEECCEEEEE
GSSFPYRNFYPEKADIIQIDLDPTQIGRRTPVALGLVGGVRDTLEALQPKLKPHTDRRFL
CCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHH
DKALKHYAKAREELDELATPTPDGAPIHPQYLTRLVDEQADADAIFTVDVGTPTLWAARY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
LHMNGKRSLLGSFNHGSMANALPQALGAKAEHPDRQVIALCGDGGLSMLLGDLLSIRQLN
HHCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
LPIKMVVFNNSSLGFVDMEMKASGYVPHGTDLHETNFAGIALGAGILGLRVENAEELPAA
CCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCEEEEEECCHHHHHHH
LRKAFDHPGPVLIDVVTAKQELGIPPKIKLAQARGFSLYMMRAIMSGRGSEVLELAKTNL
HHHHHCCCCCEEEEEEECHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]