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The map label for this gene is lpdG [H]

Identifier: 66045251

GI number: 66045251

Start: 2333942

End: 2335378

Strand: Direct

Name: lpdG [H]

Synonym: Psyr_2011

Alternate gene names: 66045251

Gene position: 2333942-2335378 (Clockwise)

Preceding gene: 66045250

Following gene: 66045252

Centisome position: 38.3

GC content: 59.57

Gene sequence:

>1437_bases
ATGTCCCAGAAATTCGACGTGGTAGTGATTGGCGCAGGCCCTGGCGGTTATGTTGCCGCCATCAAGGCTGCGCAACTTGG
TCTCAAGACTGCCTGCATCGAGAAGTATCAGGACAAAGAGGGCAAACTGGCCCTCGGCGGTACCTGCCTGAACGTGGGTT
GCATTCCTTCCAAGGCACTGCTCGACAGCTCCTGGAAATTCTATGAAGCCAAGAACGGTTTCAGCGTACATGGCATCTCC
ACCTCCGACGTGAGCATCGACGTTCCGGCGATGATCGGCCGTAAGTCGACCATCGTCAAAGGCCTGACCGGCGGCGTTGC
CAGCCTGTTCAAGGCCAACGGCGTGACCACGCTGCAGGGCCACGGCAAACTGCTGGCCGGCAAGAAAGTCGAGCTGACCG
CTGCCGACGGCACTGTCGAAATCATCGAAGCGGACCATGTGATCCTGGCTTCGGGTTCGCGTCCTATCGACATTCCACCC
GCTCCGGTTGATCAGAAAATCATCGTCGACTCGACCGGTGCTCTTGAATTCCAGCAGGTTCCACAGCGTCTGGGCGTCAT
CGGCGCTGGCGTGATCGGTCTGGAACTGGGTTCCGTGTGGGCTCGCCTGGGTGCCCAGGTCACCGTTCTGGAAGCGCTGG
ACAAGTTCATCCCGGCAGCCGACGAAGCGGTTTCCAAGGAAGCACTGAAAACCTTCAACAAGCAGGGCCTGGACATCAAG
CTGGGCGCTCGCGTGACCGGTTCCAAGGTCGAAGGCGAACAGGTTGTGGTCAGCTACACCGACGCTGCCGGCGAACAGTC
GATCACCTTCGATCGTCTGATCGTTGCCGTGGGCCGTCGTCCGGTTACCACTGACCTGCTGGCTTCGGACAGCGGTGTCG
ATCTGGACGAGCGCGGTTTCATCTACGTCGATGACTACTGCACCACCAGCGTACCGGGCGTATACGCCATCGGTGACGTG
GTTCGCGGTCTGATGCTGGCGCACAAGGCCTCGGAAGAGGGCATCATGGTTGTCGAGCGCATCAAGGGCCACAAGGCCCA
GATGAACTACAACCTGGTCCCGTCGGTTATCTACACCCACCCGGAAATCGCCTGGGTAGGCAAGACCGAACAGACCCTCA
AGGCCGAAGGCGTTGAAGTCAATGTCGGTACGTTCCCGTTCGCAGCCAGTGGCCGTGCCATGGCAGCCAACGACACCGGC
GGTTTCGTCAAGATCATTGCCGACGCCAAGACCGACCGTGTTCTGGGTGTTCACGTGATTGGCCCGAGCGCTGCCGAACT
GGTACAGCAGGGCGCAATCGCGATGGAGTTCGGTACCAGTGCAGAGGACATCGGCATGATGGTCTTCTCGCACCCGACCC
TGTCCGAGGCATTGCATGAAGCTGCACTGGCTGTGAATGGCGGCGCCATCCACATTCAGAATCGCAAGAAACGCTAA

Upstream 100 bases:

>100_bases
TTCACCTAATTCAATAGGTCGCAAGTCGGCCTCGCGATGAAACGCGGACTGACTTGCAGCTTGCAGCTAAAAGCTCGCAG
CTATAGAGGAATTCTTTTTT

Downstream 100 bases:

>100_bases
GACAACAAGAGAAACCACGACGCAGTGCCCGTCGTTGGCCTTGCAAGCAGGGCTTACCGCGGAATCTGCGCCGGACTCGA
CCTCTCAGGCGGCTTCATGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSQKFDVVVIGAGPGGYVAAIKAAQLGLKTACIEKYQDKEGKLALGGTCLNVGCIPSKALLDSSWKFYEAKNGFSVHGIS
TSDVSIDVPAMIGRKSTIVKGLTGGVASLFKANGVTTLQGHGKLLAGKKVELTAADGTVEIIEADHVILASGSRPIDIPP
APVDQKIIVDSTGALEFQQVPQRLGVIGAGVIGLELGSVWARLGAQVTVLEALDKFIPAADEAVSKEALKTFNKQGLDIK
LGARVTGSKVEGEQVVVSYTDAAGEQSITFDRLIVAVGRRPVTTDLLASDSGVDLDERGFIYVDDYCTTSVPGVYAIGDV
VRGLMLAHKASEEGIMVVERIKGHKAQMNYNLVPSVIYTHPEIAWVGKTEQTLKAEGVEVNVGTFPFAASGRAMAANDTG
GFVKIIADAKTDRVLGVHVIGPSAAELVQQGAIAMEFGTSAEDIGMMVFSHPTLSEALHEAALAVNGGAIHIQNRKKR

Sequences:

>Translated_478_residues
MSQKFDVVVIGAGPGGYVAAIKAAQLGLKTACIEKYQDKEGKLALGGTCLNVGCIPSKALLDSSWKFYEAKNGFSVHGIS
TSDVSIDVPAMIGRKSTIVKGLTGGVASLFKANGVTTLQGHGKLLAGKKVELTAADGTVEIIEADHVILASGSRPIDIPP
APVDQKIIVDSTGALEFQQVPQRLGVIGAGVIGLELGSVWARLGAQVTVLEALDKFIPAADEAVSKEALKTFNKQGLDIK
LGARVTGSKVEGEQVVVSYTDAAGEQSITFDRLIVAVGRRPVTTDLLASDSGVDLDERGFIYVDDYCTTSVPGVYAIGDV
VRGLMLAHKASEEGIMVVERIKGHKAQMNYNLVPSVIYTHPEIAWVGKTEQTLKAEGVEVNVGTFPFAASGRAMAANDTG
GFVKIIADAKTDRVLGVHVIGPSAAELVQQGAIAMEFGTSAEDIGMMVFSHPTLSEALHEAALAVNGGAIHIQNRKKR
>Mature_477_residues
SQKFDVVVIGAGPGGYVAAIKAAQLGLKTACIEKYQDKEGKLALGGTCLNVGCIPSKALLDSSWKFYEAKNGFSVHGIST
SDVSIDVPAMIGRKSTIVKGLTGGVASLFKANGVTTLQGHGKLLAGKKVELTAADGTVEIIEADHVILASGSRPIDIPPA
PVDQKIIVDSTGALEFQQVPQRLGVIGAGVIGLELGSVWARLGAQVTVLEALDKFIPAADEAVSKEALKTFNKQGLDIKL
GARVTGSKVEGEQVVVSYTDAAGEQSITFDRLIVAVGRRPVTTDLLASDSGVDLDERGFIYVDDYCTTSVPGVYAIGDVV
RGLMLAHKASEEGIMVVERIKGHKAQMNYNLVPSVIYTHPEIAWVGKTEQTLKAEGVEVNVGTFPFAASGRAMAANDTGG
FVKIIADAKTDRVLGVHVIGPSAAELVQQGAIAMEFGTSAEDIGMMVFSHPTLSEALHEAALAVNGGAIHIQNRKKR

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=50.4273504273504, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI50301238, Length=472, Percent_Identity=27.3305084745763, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI22035672, Length=472, Percent_Identity=29.0254237288136, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519430, Length=462, Percent_Identity=25.7575757575758, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI33519428, Length=462, Percent_Identity=25.7575757575758, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI33519426, Length=462, Percent_Identity=25.7575757575758, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI148277065, Length=448, Percent_Identity=25.6696428571429, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI148277071, Length=462, Percent_Identity=25.7575757575758, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI291045266, Length=441, Percent_Identity=25.8503401360544, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI291045268, Length=433, Percent_Identity=24.2494226327945, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1786307, Length=477, Percent_Identity=40.8805031446541, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI87081717, Length=463, Percent_Identity=28.7257019438445, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87082354, Length=472, Percent_Identity=30.7203389830508, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789915, Length=471, Percent_Identity=28.0254777070064, Blast_Score=171, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI32565766, Length=470, Percent_Identity=50, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=26.7368421052632, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI71983429, Length=471, Percent_Identity=28.8747346072187, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=471, Percent_Identity=28.8747346072187, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=497, Percent_Identity=23.7424547283702, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=45.850622406639, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6325240, Length=499, Percent_Identity=30.8617234468938, Blast_Score=219, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=28.0254777070064, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI21358499, Length=467, Percent_Identity=50.5353319057816, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17737741, Length=484, Percent_Identity=26.0330578512397, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640549, Length=486, Percent_Identity=26.5432098765432, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640553, Length=486, Percent_Identity=26.5432098765432, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640551, Length=486, Percent_Identity=26.5432098765432, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50086; Mature: 49955

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKFDVVVIGAGPGGYVAAIKAAQLGLKTACIEKYQDKEGKLALGGTCLNVGCIPSKAL
CCCCEEEEEEECCCCCEEEEEHHHHHCHHHHHHHHHCCCCCCEEECCEEEEECCCCCHHH
LDSSWKFYEAKNGFSVHGISTSDVSIDVPAMIGRKSTIVKGLTGGVASLFKANGVTTLQG
HCCCCEEEECCCCCEEECCCCCCCEEECHHHCCCCHHHHHHCCCHHHHHHHCCCEEEEEC
HGKLLAGKKVELTAADGTVEIIEADHVILASGSRPIDIPPAPVDQKIIVDSTGALEFQQV
CCEEEECCEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHH
PQRLGVIGAGVIGLELGSVWARLGAQVTVLEALDKFIPAADEAVSKEALKTFNKQGLDIK
HHHHCCCCCCCEEHHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEE
LGARVTGSKVEGEQVVVSYTDAAGEQSITFDRLIVAVGRRPVTTDLLASDSGVDLDERGF
ECCEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCE
IYVDDYCTTSVPGVYAIGDVVRGLMLAHKASEEGIMVVERIKGHKAQMNYNLVPSVIYTH
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCEEEEC
PEIAWVGKTEQTLKAEGVEVNVGTFPFAASGRAMAANDTGGFVKIIADAKTDRVLGVHVI
CCEEEECCCCHHHEECCEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEE
GPSAAELVQQGAIAMEFGTSAEDIGMMVFSHPTLSEALHEAALAVNGGAIHIQNRKKR
CCCHHHHHHCCCEEEEECCCHHHCEEEEECCCCHHHHHHHHHEEECCCEEEECCCCCC
>Mature Secondary Structure 
SQKFDVVVIGAGPGGYVAAIKAAQLGLKTACIEKYQDKEGKLALGGTCLNVGCIPSKAL
CCCEEEEEEECCCCCEEEEEHHHHHCHHHHHHHHHCCCCCCEEECCEEEEECCCCCHHH
LDSSWKFYEAKNGFSVHGISTSDVSIDVPAMIGRKSTIVKGLTGGVASLFKANGVTTLQG
HCCCCEEEECCCCCEEECCCCCCCEEECHHHCCCCHHHHHHCCCHHHHHHHCCCEEEEEC
HGKLLAGKKVELTAADGTVEIIEADHVILASGSRPIDIPPAPVDQKIIVDSTGALEFQQV
CCEEEECCEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHH
PQRLGVIGAGVIGLELGSVWARLGAQVTVLEALDKFIPAADEAVSKEALKTFNKQGLDIK
HHHHCCCCCCCEEHHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEE
LGARVTGSKVEGEQVVVSYTDAAGEQSITFDRLIVAVGRRPVTTDLLASDSGVDLDERGF
ECCEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCE
IYVDDYCTTSVPGVYAIGDVVRGLMLAHKASEEGIMVVERIKGHKAQMNYNLVPSVIYTH
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCEEEEC
PEIAWVGKTEQTLKAEGVEVNVGTFPFAASGRAMAANDTGGFVKIIADAKTDRVLGVHVI
CCEEEECCCCHHHEECCEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEE
GPSAAELVQQGAIAMEFGTSAEDIGMMVFSHPTLSEALHEAALAVNGGAIHIQNRKKR
CCCHHHHHHCCCEEEEECCCHHHCEEEEECCCCHHHHHHHHHEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1902462; 2914869 [H]