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The map label for this gene is sucB [H]

Identifier: 66045250

GI number: 66045250

Start: 2332534

End: 2333769

Strand: Direct

Name: sucB [H]

Synonym: Psyr_2010

Alternate gene names: 66045250

Gene position: 2332534-2333769 (Clockwise)

Preceding gene: 66045249

Following gene: 66045251

Centisome position: 38.28

GC content: 58.66

Gene sequence:

>1236_bases
ATGGCTATCGAGATCAAAGCCCCCTCTTTCCCGGAATCCGTTGCCGACGGCACCATTTCCAAGTGGTACAAAAAAGAGGG
CGATGCGGTAAAGCGCGACGAGATGCTGGTCGATATCGAGACTGACAAGGTTGTCCTCGAAGTGCTGGCCGAAGCTGACG
GCGTGATGGGAACGATCACCAAGGAAGAGGGTGCCATCGTCCTGTCCAACGAAGTGCTTGGGACATTGAATGACGGTGCT
ACAGCATCTGCTGCTCCGGCACCTGCTGCTGCGCCTGCCGCGGCACCTGCTTCGGCACCCGCCGCTGCACCGGCTGCAAC
TGCTGGCGAAGAAGACCCGATTGCTGCGCCTGCTGCGCGTCAACTGGCTGAAGAAAACGGCATCAACCTGGCCAGCGTCA
AGGGCACTGGTAAAGACGGCCGTATCACCAAGGAAGACATCGTTGCTGCCGTTGAAGCGAAGAAATCCGCTCCGGCCGCT
GCGCCTGCTGCCAAGCCTGCTGCTGCCGCTGCTCCTGTCGTCGCCGCTGGCGATCGCACCGAGAAGCGTGTACCGATGAC
TCGCGTGCGTGCAACGGTTGCCAAGCGTCTGGTCGAAGCCCAGTCGAACATGGCGATGCTGACCACGTTCAACGAAGTCG
ACATGACCGAAGTCATGGCACTGCGTTCCAAGTACAAGGACCTGTTCGAGAAGTCGCACAACGGCGTACGCCTGGGCTTC
ATGTCGTTCTTCGTCAAGGCTGCCACCGAGGCGCTGAAACGCTTCCCGGCCGTCAACGCGTCGATCGACGGTTCCGATAT
CGTTTACCACGGCTATGCCGACGTCGGTGTCGCGGTTTCCAGCGATCGCGGTCTGGTCGTACCGGTTCTGCGTAACGCAG
AGCACATGAGCCTGGCTGAAATCGAAGGCGGCATCGCCACCTTCGGCAAGAAAGCCCGTGACGGTAAACTGTCCATCGAT
GAGATGACCGGCGGTACGTTCACCATCACCAATGGTGGTACTTTCGGTTCGATGATGTCGACGCCGATCGTCAACCCGCC
ACAGGCTGCAATTCTGGGCATGCACAACATTCTGCAGCGTCCGATGGCAGTCAATGGTCAGGTTGTGATTCGCCCGATGA
TGTATCTGGCGTTGTCCTACGATCACCGTTTGATCGACGGCAAGGAAGCGGTAACCTTCCTCGTGACCATCAAGAACCTG
CTGGAAGATCCTGCTCGTCTGCTGCTGGATATCTGA

Upstream 100 bases:

>100_bases
ACGCTTCGATGCACGCCGAGCAGCAGGAAAAACTGCTGCAAGATGCCTTCACTGTTTAACGCCTTCGCGCATTAGAAACC
GAATTAAGGAATTACAGATA

Downstream 100 bases:

>100_bases
TGGAGTAGCCTCGAGCTGCAAGCTTCAAGCTTCAAGTCAGAAGCGAAAGGCGAGCAGTCGGGGCTGATGTGTTTCACCTA
ATTCAATAGGTCGCAAGTCG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MAIEIKAPSFPESVADGTISKWYKKEGDAVKRDEMLVDIETDKVVLEVLAEADGVMGTITKEEGAIVLSNEVLGTLNDGA
TASAAPAPAAAPAAAPASAPAAAPAATAGEEDPIAAPAARQLAEENGINLASVKGTGKDGRITKEDIVAAVEAKKSAPAA
APAAKPAAAAAPVVAAGDRTEKRVPMTRVRATVAKRLVEAQSNMAMLTTFNEVDMTEVMALRSKYKDLFEKSHNGVRLGF
MSFFVKAATEALKRFPAVNASIDGSDIVYHGYADVGVAVSSDRGLVVPVLRNAEHMSLAEIEGGIATFGKKARDGKLSID
EMTGGTFTITNGGTFGSMMSTPIVNPPQAAILGMHNILQRPMAVNGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNL
LEDPARLLLDI

Sequences:

>Translated_411_residues
MAIEIKAPSFPESVADGTISKWYKKEGDAVKRDEMLVDIETDKVVLEVLAEADGVMGTITKEEGAIVLSNEVLGTLNDGA
TASAAPAPAAAPAAAPASAPAAAPAATAGEEDPIAAPAARQLAEENGINLASVKGTGKDGRITKEDIVAAVEAKKSAPAA
APAAKPAAAAAPVVAAGDRTEKRVPMTRVRATVAKRLVEAQSNMAMLTTFNEVDMTEVMALRSKYKDLFEKSHNGVRLGF
MSFFVKAATEALKRFPAVNASIDGSDIVYHGYADVGVAVSSDRGLVVPVLRNAEHMSLAEIEGGIATFGKKARDGKLSID
EMTGGTFTITNGGTFGSMMSTPIVNPPQAAILGMHNILQRPMAVNGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNL
LEDPARLLLDI
>Mature_410_residues
AIEIKAPSFPESVADGTISKWYKKEGDAVKRDEMLVDIETDKVVLEVLAEADGVMGTITKEEGAIVLSNEVLGTLNDGAT
ASAAPAPAAAPAAAPASAPAAAPAATAGEEDPIAAPAARQLAEENGINLASVKGTGKDGRITKEDIVAAVEAKKSAPAAA
PAAKPAAAAAPVVAAGDRTEKRVPMTRVRATVAKRLVEAQSNMAMLTTFNEVDMTEVMALRSKYKDLFEKSHNGVRLGFM
SFFVKAATEALKRFPAVNASIDGSDIVYHGYADVGVAVSSDRGLVVPVLRNAEHMSLAEIEGGIATFGKKARDGKLSIDE
MTGGTFTITNGGTFGSMMSTPIVNPPQAAILGMHNILQRPMAVNGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNLL
EDPARLLLDI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=55.9055118110236, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=30.5361305361305, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=30.1098901098901, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI203098816, Length=455, Percent_Identity=30.1098901098901, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=30.4347826086957, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.6459627329193, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1786946, Length=411, Percent_Identity=56.9343065693431, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1786305, Length=302, Percent_Identity=36.4238410596026, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI25146366, Length=426, Percent_Identity=42.7230046948357, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=31.4350797266515, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=26.2295081967213, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17538894, Length=326, Percent_Identity=30.0613496932515, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320352, Length=415, Percent_Identity=42.8915662650602, Blast_Score=321, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=28.6666666666667, Blast_Score=152, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=57.6419213973799, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI18859875, Length=420, Percent_Identity=30, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24582497, Length=299, Percent_Identity=31.438127090301, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI20129315, Length=299, Percent_Identity=31.438127090301, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43022; Mature: 42891

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEIKAPSFPESVADGTISKWYKKEGDAVKRDEMLVDIETDKVVLEVLAEADGVMGTIT
CEEEECCCCCCHHHHCCHHHHHHHHCCCCEECCCEEEEECCHHHHHHHHHHCCCCEEEEE
KEEGAIVLSNEVLGTLNDGATASAAPAPAAAPAAAPASAPAAAPAATAGEEDPIAAPAAR
CCCCCEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
QLAEENGINLASVKGTGKDGRITKEDIVAAVEAKKSAPAAAPAAKPAAAAAPVVAAGDRT
HHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEECCCCC
EKRVPMTRVRATVAKRLVEAQSNMAMLTTFNEVDMTEVMALRSKYKDLFEKSHNGVRLGF
CCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEHH
MSFFVKAATEALKRFPAVNASIDGSDIVYHGYADVGVAVSSDRGLVVPVLRNAEHMSLAE
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEEEEECCCCCCCHHH
IEGGIATFGKKARDGKLSIDEMTGGTFTITNGGTFGSMMSTPIVNPPQAAILGMHNILQR
HCCCHHHHCCCCCCCCEEEEECCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHCC
PMAVNGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNLLEDPARLLLDI
CCCCCCCEEEHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
AIEIKAPSFPESVADGTISKWYKKEGDAVKRDEMLVDIETDKVVLEVLAEADGVMGTIT
EEEECCCCCCHHHHCCHHHHHHHHCCCCEECCCEEEEECCHHHHHHHHHHCCCCEEEEE
KEEGAIVLSNEVLGTLNDGATASAAPAPAAAPAAAPASAPAAAPAATAGEEDPIAAPAAR
CCCCCEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
QLAEENGINLASVKGTGKDGRITKEDIVAAVEAKKSAPAAAPAAKPAAAAAPVVAAGDRT
HHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEECCCCC
EKRVPMTRVRATVAKRLVEAQSNMAMLTTFNEVDMTEVMALRSKYKDLFEKSHNGVRLGF
CCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEHH
MSFFVKAATEALKRFPAVNASIDGSDIVYHGYADVGVAVSSDRGLVVPVLRNAEHMSLAE
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEEEEECCCCCCCHHH
IEGGIATFGKKARDGKLSIDEMTGGTFTITNGGTFGSMMSTPIVNPPQAAILGMHNILQR
HCCCHHHHCCCCCCCCEEEEECCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHCC
PMAVNGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNLLEDPARLLLDI
CCCCCCCEEEHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]