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The map label for this gene is leuD [H]

Identifier: 66045224

GI number: 66045224

Start: 2304159

End: 2304800

Strand: Direct

Name: leuD [H]

Synonym: Psyr_1984

Alternate gene names: 66045224

Gene position: 2304159-2304800 (Clockwise)

Preceding gene: 66045223

Following gene: 66045225

Centisome position: 37.81

GC content: 57.63

Gene sequence:

>642_bases
ATGAAAGCCTTTACCCAGCACCATGGCCTGGTCGCTCCTCTGGATCGTGCCAATGTCGACACCGACCAGATCATTCCCAA
GCAGTTTCTCAAGTCGATCAAGCGCACGGGTTTTGGCCCGAACCTGTTCGATGAATGGCGTTATCTGGATGTCGGCCAGC
CGTATCAGGACAACTCCAAGCGCCCGCTGAACCCTGACTTCGTGCTCAATCATGAGCGGTATCAGGGTGCCAGCGTGCTG
TTGGCCCGGGAGAACTTCGGTTGCGGTTCCAGCCGCGAACACGCGCCTTGGGCACTGGAAGAGTACGGTTTCTGCGCAAT
CATTGCGCCGAGCTACGCGGACATCTTCTTCAATAACAGCTTCAAGAACGGCTTGTTGCCGATCATCCTGTCCGAGGAAG
ACGTTGATCAACTATTCAAGCAGGTGGAAGCCTCGCCCGGTTATCAGTTGAGTATCGATCTGCAGGCGCAGACCGTGACC
CGTCCGGATGGCAAGGTCCTGAGCTTTGAAATCGATGCGTTCCGCAAGCATTGCCTGCTCAATGGTCTGGACGACATCGG
CCTGACGCTGATGGACGCCGAGGCCATCGCCGGTTTCGAGAGCAGGCACCGCGCCAGCCAGCCGTGGTTGTTCCGCGACT
GA

Upstream 100 bases:

>100_bases
GGCCGGACGCATCTGGTGAGTCCGGCGATGGCCGCTGCCGCTGCAGTGAATGGCCGTTTCATCGATGTTCGCGACCTGAT
CCAGCACTGAGGAGTCCCGT

Downstream 100 bases:

>100_bases
TCGATTGATCGATCGACAGCGGATGCATGTTTGCCAGTAAGGTGTAATTGCGCCTTGCTGGCAAAAGTGTTTTGGCCGGA
CGCTAAATTTTCTGTGGTAT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKAFTQHHGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSKRPLNPDFVLNHERYQGASVL
LARENFGCGSSREHAPWALEEYGFCAIIAPSYADIFFNNSFKNGLLPIILSEEDVDQLFKQVEASPGYQLSIDLQAQTVT
RPDGKVLSFEIDAFRKHCLLNGLDDIGLTLMDAEAIAGFESRHRASQPWLFRD

Sequences:

>Translated_213_residues
MKAFTQHHGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSKRPLNPDFVLNHERYQGASVL
LARENFGCGSSREHAPWALEEYGFCAIIAPSYADIFFNNSFKNGLLPIILSEEDVDQLFKQVEASPGYQLSIDLQAQTVT
RPDGKVLSFEIDAFRKHCLLNGLDDIGLTLMDAEAIAGFESRHRASQPWLFRD
>Mature_213_residues
MKAFTQHHGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSKRPLNPDFVLNHERYQGASVL
LARENFGCGSSREHAPWALEEYGFCAIIAPSYADIFFNNSFKNGLLPIILSEEDVDQLFKQVEASPGYQLSIDLQAQTVT
RPDGKVLSFEIDAFRKHCLLNGLDDIGLTLMDAEAIAGFESRHRASQPWLFRD

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=202, Percent_Identity=59.9009900990099, Blast_Score=250, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6321429, Length=214, Percent_Identity=52.803738317757, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 24134; Mature: 24134

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFTQHHGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSK
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCCCC
RPLNPDFVLNHERYQGASVLLARENFGCGSSREHAPWALEEYGFCAIIAPSYADIFFNNS
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEHHHCCEEEEECCCCEEEEECCC
FKNGLLPIILSEEDVDQLFKQVEASPGYQLSIDLQAQTVTRPDGKVLSFEIDAFRKHCLL
CCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEEEEEHHHHHHHHHH
NGLDDIGLTLMDAEAIAGFESRHRASQPWLFRD
CCCCCCCEEEECHHHHHCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MKAFTQHHGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSK
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCCCC
RPLNPDFVLNHERYQGASVLLARENFGCGSSREHAPWALEEYGFCAIIAPSYADIFFNNS
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEHHHCCEEEEECCCCEEEEECCC
FKNGLLPIILSEEDVDQLFKQVEASPGYQLSIDLQAQTVTRPDGKVLSFEIDAFRKHCLL
CCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEEEEEHHHHHHHHHH
NGLDDIGLTLMDAEAIAGFESRHRASQPWLFRD
CCCCCCCEEEECHHHHHCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]