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The map label for this gene is leuB [H]

Identifier: 66045225

GI number: 66045225

Start: 2304956

End: 2306038

Strand: Direct

Name: leuB [H]

Synonym: Psyr_1985

Alternate gene names: 66045225

Gene position: 2304956-2306038 (Clockwise)

Preceding gene: 66045224

Following gene: 66045226

Centisome position: 37.83

GC content: 61.03

Gene sequence:

>1083_bases
ATGAGCAAGCAGATTCTGATTCTCCCAGGTGACGGTATTGGTCCGGAAATCATGACCGAAGCGGTCAAGGTGCTGGAGCT
GGCCAACGAAAAGTATCAACTGGGCTTTGAATTGACCCACGACGTGATCGGCGGCGCGGCCATCGACAAGCACGGCGTGC
CGCTGGCCGATGAAACCCTGGAGCGTGCCCGTGCGGCCGACGCCGTGCTGCTCGGCGCAGTCGGCGGACCGAAATGGGAC
ACCATCGAGCGTGACATCCGTCCTGAGCGCGGTCTGCTGAAAATCCGTTCGCAACTGGGCCTGTTCGGCAACCTGCGTCC
GGCGATCCTTTATCCGCAACTGGCCGACGCGTCGAGCCTGAAGCCGGAAATCGTCGCCGGCCTGGATATCATGATCGTTC
GCGAGCTGACCGGTGGCATCTATTTCGGCGCGCCACGCGGCACGCGGGTGCTGGATAATGGCGAGCGTCAGGCTTACGAC
ACGCTGCCTTACAGCGAAAGCGAGATCCGCCGCATTGCCAAGGTCGGTTTCGACATGGCCATGGTGCGTGGCAAGAAGCT
CTGCTCGGTGGACAAGGCCAACGTGCTGGCGTCCAGCCAGCTGTGGCGCGAAATCGTCGAGCAGGTCGCCAGGGATTACC
CTGAAGTCGAACTGAGCCACATGTACGTCGACAACGCTGCCATGCAACTGGTGCGTGCACCCAAGCAGTTCGATGTGATC
GTGACCGACAACCTGTTCGGCGACATCCTCTCCGATCAGGCATCGATGCTCACCGGCTCCATCGGCATGCTGCCGTCGGC
CTCGCTGGACACGGCCAACAAGGGCATGTACGAGCCATGTCACGGTTCGGCGCCGGACATCGCCGGCAAAGGCATCGCCA
ACCCGTTGGCGACCATTCTCTCGGTGTCGATGATGCTGCGTTACAGCTTCAATCTGACCGATGCGGCCGATGCCATCGAA
AAGGCTGTCAGCCTGGTTCTGGATCAGGGTATCCGCACCGGCGACATCTGGTCGGAAGGCAAAGTCAAAGTCGGTACGCA
GGAAATGGGCGATGCGGTAGTCGCCGCGCTGCGGAATCTGTAA

Upstream 100 bases:

>100_bases
TGCTGGCAAAAGTGTTTTGGCCGGACGCTAAATTTTCTGTGGTATTGAGCGTTTCAAGCAGGCGTTGTCAAAGACGCCGA
TGCACAAAATCGAGGATGTT

Downstream 100 bases:

>100_bases
TCTCTCTGGCCCGCCGCCTGCTGTGTACGCGCAGAGCGGCGGTCCCACTTTTATTCAAGGTGTAGTTGCGATGAAACGTG
TAGGTCTGATCGGTTGGCGT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSKQILILPGDGIGPEIMTEAVKVLELANEKYQLGFELTHDVIGGAAIDKHGVPLADETLERARAADAVLLGAVGGPKWD
TIERDIRPERGLLKIRSQLGLFGNLRPAILYPQLADASSLKPEIVAGLDIMIVRELTGGIYFGAPRGTRVLDNGERQAYD
TLPYSESEIRRIAKVGFDMAMVRGKKLCSVDKANVLASSQLWREIVEQVARDYPEVELSHMYVDNAAMQLVRAPKQFDVI
VTDNLFGDILSDQASMLTGSIGMLPSASLDTANKGMYEPCHGSAPDIAGKGIANPLATILSVSMMLRYSFNLTDAADAIE
KAVSLVLDQGIRTGDIWSEGKVKVGTQEMGDAVVAALRNL

Sequences:

>Translated_360_residues
MSKQILILPGDGIGPEIMTEAVKVLELANEKYQLGFELTHDVIGGAAIDKHGVPLADETLERARAADAVLLGAVGGPKWD
TIERDIRPERGLLKIRSQLGLFGNLRPAILYPQLADASSLKPEIVAGLDIMIVRELTGGIYFGAPRGTRVLDNGERQAYD
TLPYSESEIRRIAKVGFDMAMVRGKKLCSVDKANVLASSQLWREIVEQVARDYPEVELSHMYVDNAAMQLVRAPKQFDVI
VTDNLFGDILSDQASMLTGSIGMLPSASLDTANKGMYEPCHGSAPDIAGKGIANPLATILSVSMMLRYSFNLTDAADAIE
KAVSLVLDQGIRTGDIWSEGKVKVGTQEMGDAVVAALRNL
>Mature_359_residues
SKQILILPGDGIGPEIMTEAVKVLELANEKYQLGFELTHDVIGGAAIDKHGVPLADETLERARAADAVLLGAVGGPKWDT
IERDIRPERGLLKIRSQLGLFGNLRPAILYPQLADASSLKPEIVAGLDIMIVRELTGGIYFGAPRGTRVLDNGERQAYDT
LPYSESEIRRIAKVGFDMAMVRGKKLCSVDKANVLASSQLWREIVEQVARDYPEVELSHMYVDNAAMQLVRAPKQFDVIV
TDNLFGDILSDQASMLTGSIGMLPSASLDTANKGMYEPCHGSAPDIAGKGIANPLATILSVSMMLRYSFNLTDAADAIEK
AVSLVLDQGIRTGDIWSEGKVKVGTQEMGDAVVAALRNL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=351, Percent_Identity=31.0541310541311, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI28178816, Length=363, Percent_Identity=29.2011019283747, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=30.0319488817891, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4758582, Length=352, Percent_Identity=30.3977272727273, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI28178838, Length=331, Percent_Identity=30.8157099697885, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28178819, Length=171, Percent_Identity=36.2573099415205, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=55.1820728291317, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788101, Length=365, Percent_Identity=35.8904109589041, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1787381, Length=397, Percent_Identity=25.6926952141058, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=353, Percent_Identity=31.728045325779, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17505779, Length=373, Percent_Identity=28.686327077748, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=361, Percent_Identity=29.6398891966759, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=352, Percent_Identity=29.5454545454545, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=48.3783783783784, Blast_Score=320, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6322097, Length=375, Percent_Identity=37.3333333333333, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324291, Length=334, Percent_Identity=30.2395209580838, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324709, Length=339, Percent_Identity=30.9734513274336, Blast_Score=115, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24643268, Length=354, Percent_Identity=33.0508474576271, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24643270, Length=354, Percent_Identity=33.0508474576271, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24661184, Length=365, Percent_Identity=32.8767123287671, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI281362242, Length=356, Percent_Identity=27.8089887640449, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648872, Length=356, Percent_Identity=27.8089887640449, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078637, Length=342, Percent_Identity=27.7777777777778, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161078633, Length=342, Percent_Identity=27.7777777777778, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24650122, Length=342, Percent_Identity=27.7777777777778, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161078635, Length=342, Percent_Identity=27.7777777777778, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161078639, Length=340, Percent_Identity=27.9411764705882, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI20130355, Length=360, Percent_Identity=23.0555555555556, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38877; Mature: 38746

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQILILPGDGIGPEIMTEAVKVLELANEKYQLGFELTHDVIGGAAIDKHGVPLADETL
CCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHEECHHHHHHHHCCCCCCCCCCCCHHHHH
ERARAADAVLLGAVGGPKWDTIERDIRPERGLLKIRSQLGLFGNLRPAILYPQLADASSL
HHHHHHCEEEEECCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCCC
KPEIVAGLDIMIVRELTGGIYFGAPRGTRVLDNGERQAYDTLPYSESEIRRIAKVGFDMA
CCHHHHCCHHEEEEHHHCCEEECCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHH
MVRGKKLCSVDKANVLASSQLWREIVEQVARDYPEVELSHMYVDNAAMQLVRAPKQFDVI
HHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHEEHHHHHHHHHHCCCCEEEE
VTDNLFGDILSDQASMLTGSIGMLPSASLDTANKGMYEPCHGSAPDIAGKGIANPLATIL
EECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVSMMLRYSFNLTDAADAIEKAVSLVLDQGIRTGDIWSEGKVKVGTQEMGDAVVAALRNL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKQILILPGDGIGPEIMTEAVKVLELANEKYQLGFELTHDVIGGAAIDKHGVPLADETL
CCCEEEECCCCCCHHHHHHHHHHHHHHCCHHEECHHHHHHHHCCCCCCCCCCCCHHHHH
ERARAADAVLLGAVGGPKWDTIERDIRPERGLLKIRSQLGLFGNLRPAILYPQLADASSL
HHHHHHCEEEEECCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCCC
KPEIVAGLDIMIVRELTGGIYFGAPRGTRVLDNGERQAYDTLPYSESEIRRIAKVGFDMA
CCHHHHCCHHEEEEHHHCCEEECCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHH
MVRGKKLCSVDKANVLASSQLWREIVEQVARDYPEVELSHMYVDNAAMQLVRAPKQFDVI
HHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHEEHHHHHHHHHHCCCCEEEE
VTDNLFGDILSDQASMLTGSIGMLPSASLDTANKGMYEPCHGSAPDIAGKGIANPLATIL
EECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVSMMLRYSFNLTDAADAIEKAVSLVLDQGIRTGDIWSEGKVKVGTQEMGDAVVAALRNL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA