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The map label for this gene is leuC [H]

Identifier: 66045223

GI number: 66045223

Start: 2302724

End: 2304148

Strand: Direct

Name: leuC [H]

Synonym: Psyr_1983

Alternate gene names: 66045223

Gene position: 2302724-2304148 (Clockwise)

Preceding gene: 66045220

Following gene: 66045224

Centisome position: 37.79

GC content: 62.53

Gene sequence:

>1425_bases
ATGGCCGGGAAAACGCTCTACGACAAGCTCTGGGATTCGCATTTGGTCAAACAGCGCGACGACGGTTCGGCGCTGATCTA
CATTGACCGTCATATCATCCATGAAGTGACCTCGCCGCAGGCGTTCGAAGGTCTGCGGCTGGCGAAGCGCAAGCCGTGGC
GGATCGACTCGATCATCGCCACGCCTGACCATAACGTGCCGACCACGGCCGAACGCAAGGGTGGCATCGGTGCCATCGAG
GATCAGGTGTCGCGCTTGCAGGTGCAGACCCTCGACGACAACTGCGACGAGTACGGCATCACCGAATTCAAGATGAATGA
CCCGCGCCAGGGCATCGTCCATGTGATCGGCCCGGAGCAGGGCGCAACCCTGCCAGGCATGAGCGTGGTCTGCGGCGACT
CGCACACCTCTACCCACGGTGCATTCGGTGCGCTGGCCCACGGTATCGGCACTTCCGAGGTCGAGCACGTGCTCGCCACC
CAGTGCCTGGTCGCCAAGAAAATGAAGAACATGCTGGTGTCGGTCGAAGGGCAATTGCCGTTCGGCGTGACGGCCAAGGA
CATCGTGCTGGCGGTGATCGGCAAGATCGGCACCGCAGGCGGTAACGGTTACGCCATCGAGTTCGCCGGCAGCGCGATTC
GCGACCTGTCGATCGAAGGGCGCATGACCATCTGCAACATGTCCATCGAAGCGGGTGCCCGGGTCGGCATGGTGGCCACC
GACGAGAAGACCGTCGAGTACGTCAAAGGTCGTCCGTTCGCACCCAAGGGTGCAGAGTGGGACCTCGCTGTCGAAGCCTG
GAAGGACCTGGTGTCCGACCCGGACGCTGTGTTCGATACCGTGGTCAGGCTGGACGCCGCACAGATCAAGCCGCAGGTCA
GCTGGGGCACCTCGCCGGAAATGGTCCTCGCGGTCGATCAGAACGTGCCGGACCCTGCGCAAGAGCCGGATCTGGTCAAG
CGCGGCTCCATCGAGCGTGCGCTCAAGTACATGGGCCTGAAAGCCAATCAGCCGATTACCGATATTCAACTGGACCGGGT
ATTCATCGGTTCCTGCACCAACTCGCGCATCGAAGATTTGCGTGCTGCTGCGGATGTCGCCAAGGGCCGCAAGGTGGCTT
CAACCATCAAGCAGGCCATCGTGGTGCCGGGTTCGGGGCTGATCAAGGCGCAGGCCGAGAAGGAAGGGCTGGACAAGGTG
TTCATCGAAGCCGGCTTCGAATGGCGTGAACCGGGCTGCTCCATGTGCCTGGCGATGAACCCGGACCGCCTGGGTTCCGG
CGAGCATTGCGCGTCGACCTCCAACCGTAACTTCGAAGGTCGTCAGGGCGCCGGTGGCCGGACGCATCTGGTGAGTCCGG
CGATGGCCGCTGCCGCTGCAGTGAATGGCCGTTTCATCGATGTTCGCGACCTGATCCAGCACTGA

Upstream 100 bases:

>100_bases
GAGTTATTTAAGCATATTCCATAGGATGACCGCACAAGCCAAAGGGTCATCGCCACGATTGCCCAAGGCATAGAAACAAG
CTGATGAGGAAGTACGTCCG

Downstream 100 bases:

>100_bases
GGAGTCCCGTATGAAAGCCTTTACCCAGCACCATGGCCTGGTCGCTCCTCTGGATCGTGCCAATGTCGACACCGACCAGA
TCATTCCCAAGCAGTTTCTC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MAGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAKRKPWRIDSIIATPDHNVPTTAERKGGIGAIE
DQVSRLQVQTLDDNCDEYGITEFKMNDPRQGIVHVIGPEQGATLPGMSVVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
QCLVAKKMKNMLVSVEGQLPFGVTAKDIVLAVIGKIGTAGGNGYAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGMVAT
DEKTVEYVKGRPFAPKGAEWDLAVEAWKDLVSDPDAVFDTVVRLDAAQIKPQVSWGTSPEMVLAVDQNVPDPAQEPDLVK
RGSIERALKYMGLKANQPITDIQLDRVFIGSCTNSRIEDLRAAADVAKGRKVASTIKQAIVVPGSGLIKAQAEKEGLDKV
FIEAGFEWREPGCSMCLAMNPDRLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFIDVRDLIQH

Sequences:

>Translated_474_residues
MAGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAKRKPWRIDSIIATPDHNVPTTAERKGGIGAIE
DQVSRLQVQTLDDNCDEYGITEFKMNDPRQGIVHVIGPEQGATLPGMSVVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
QCLVAKKMKNMLVSVEGQLPFGVTAKDIVLAVIGKIGTAGGNGYAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGMVAT
DEKTVEYVKGRPFAPKGAEWDLAVEAWKDLVSDPDAVFDTVVRLDAAQIKPQVSWGTSPEMVLAVDQNVPDPAQEPDLVK
RGSIERALKYMGLKANQPITDIQLDRVFIGSCTNSRIEDLRAAADVAKGRKVASTIKQAIVVPGSGLIKAQAEKEGLDKV
FIEAGFEWREPGCSMCLAMNPDRLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFIDVRDLIQH
>Mature_473_residues
AGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAKRKPWRIDSIIATPDHNVPTTAERKGGIGAIED
QVSRLQVQTLDDNCDEYGITEFKMNDPRQGIVHVIGPEQGATLPGMSVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQ
CLVAKKMKNMLVSVEGQLPFGVTAKDIVLAVIGKIGTAGGNGYAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGMVATD
EKTVEYVKGRPFAPKGAEWDLAVEAWKDLVSDPDAVFDTVVRLDAAQIKPQVSWGTSPEMVLAVDQNVPDPAQEPDLVKR
GSIERALKYMGLKANQPITDIQLDRVFIGSCTNSRIEDLRAAADVAKGRKVASTIKQAIVVPGSGLIKAQAEKEGLDKVF
IEAGFEWREPGCSMCLAMNPDRLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFIDVRDLIQH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=378, Percent_Identity=28.3068783068783, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=63.4615384615385, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1787531, Length=456, Percent_Identity=24.780701754386, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081781, Length=354, Percent_Identity=24.8587570621469, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367097, Length=345, Percent_Identity=25.7971014492754, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=382, Percent_Identity=28.0104712041885, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=384, Percent_Identity=28.3854166666667, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=306, Percent_Identity=26.797385620915, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=403, Percent_Identity=27.7915632754342, Blast_Score=95, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=60.8510638297872, Blast_Score=587, Evalue=1e-168,
Organism=Saccharomyces cerevisiae, GI6323335, Length=376, Percent_Identity=29.5212765957447, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6320440, Length=431, Percent_Identity=27.1461716937355, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=395, Percent_Identity=28.6075949367089, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281365315, Length=383, Percent_Identity=28.1984334203655, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17864292, Length=383, Percent_Identity=28.1984334203655, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161076999, Length=387, Percent_Identity=28.1653746770026, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=383, Percent_Identity=26.3707571801567, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645686, Length=412, Percent_Identity=27.1844660194175, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137564, Length=372, Percent_Identity=27.6881720430108, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50969; Mature: 50837

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAKRKPWRIDSIIA
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEE
TPDHNVPTTAERKGGIGAIEDQVSRLQVQTLDDNCDEYGITEFKMNDPRQGIVHVIGPEQ
CCCCCCCCCCHHCCCCCHHHHHHHHEEEEECCCCCCCCCCEEEECCCCCCCEEEEECCCC
GATLPGMSVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVAKKMKNMLVSVEGQLP
CCCCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
FGVTAKDIVLAVIGKIGTAGGNGYAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGMVAT
CCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHEEECCCCEEEEEEECCCCCEEEEEEC
DEKTVEYVKGRPFAPKGAEWDLAVEAWKDLVSDPDAVFDTVVRLDAAQIKPQVSWGTSPE
CHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
MVLAVDQNVPDPAQEPDLVKRGSIERALKYMGLKANQPITDIQLDRVFIGSCTNSRIEDL
EEEEECCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCCCCEEECEEEEECCCCHHHHHH
RAAADVAKGRKVASTIKQAIVVPGSGLIKAQAEKEGLDKVFIEAGFEWREPGCSMCLAMN
HHHHHHHCCHHHHHHHHHEEECCCCCCEEECHHHCCHHEEHHHCCCCCCCCCCCEEEEEC
PDRLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFIDVRDLIQH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEHHHHHCC
>Mature Secondary Structure 
AGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAKRKPWRIDSIIA
CCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEE
TPDHNVPTTAERKGGIGAIEDQVSRLQVQTLDDNCDEYGITEFKMNDPRQGIVHVIGPEQ
CCCCCCCCCCHHCCCCCHHHHHHHHEEEEECCCCCCCCCCEEEECCCCCCCEEEEECCCC
GATLPGMSVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVAKKMKNMLVSVEGQLP
CCCCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
FGVTAKDIVLAVIGKIGTAGGNGYAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGMVAT
CCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHEEECCCCEEEEEEECCCCCEEEEEEC
DEKTVEYVKGRPFAPKGAEWDLAVEAWKDLVSDPDAVFDTVVRLDAAQIKPQVSWGTSPE
CHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
MVLAVDQNVPDPAQEPDLVKRGSIERALKYMGLKANQPITDIQLDRVFIGSCTNSRIEDL
EEEEECCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCCCCEEECEEEEECCCCHHHHHH
RAAADVAKGRKVASTIKQAIVVPGSGLIKAQAEKEGLDKVFIEAGFEWREPGCSMCLAMN
HHHHHHHCCHHHHHHHHHEEECCCCCCEEECHHHCCHHEEHHHCCCCCCCCCCCEEEEEC
PDRLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFIDVRDLIQH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]