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The map label for this gene is proP [H]

Identifier: 66044922

GI number: 66044922

Start: 1885684

End: 1887021

Strand: Direct

Name: proP [H]

Synonym: Psyr_1677

Alternate gene names: 66044922

Gene position: 1885684-1887021 (Clockwise)

Preceding gene: 66044921

Following gene: 66044926

Centisome position: 30.94

GC content: 62.56

Gene sequence:

>1338_bases
ATGACCACTACTACCGTAGAGCAAGTCGACCCTGCCACGCTGAAAAAAGTCATCGTCGCTGCGGCCATCGGCAACTTCGT
CGAGTGGTTCGATTTTGCCGTCTATGGATTTCTGGCGACGACGATCGCGCTGCAGTTTTTCCCCAGCGGCGACAGCAGTG
CAGCGTTGTTGAAAACCTTTGCCGTGTTCGCCGTGGCCTTTGCATTCAGACCGCTGGGCGGGATTTTCTTCGGCATGCTG
GGTGACCGGATCGGGCGCAAGAAAACCCTGGCCATGACCATCCTGCTGATGGCCGGCGCCACCACACTGATCGGCCTGTT
GCCGACCTATGCCGCGATCGGTGTGATGGCACCGGTGCTGCTGACCATCATTCGCTGTGCGCAGGGTTTCTCGGCCGGCG
GCGAGTATGCCGGTGCCTGCGCGTACCTCATGGAGCACGCGCCGCGCACGCAACGTGCCTGGTACGGCAGCTTCGTTCCG
GTCTCGACGTTCTCCGCATTCGCTGCCGCAGCAGTAGTGGCCTACGCGCTTGAGTCGTTGCTGTCGACCGAGGCCATGGG
CAACTGGGGCTGGCGTCTGCCGTTTCTGATTGCTGCGCCGCTGGGGCTGGTCGGGCTGTACCTGCGCTGGAAGCTCGATG
AAACCCCGGCGTTTCAGGCGGTTGCTCAAGAACACGCCGTGGCTCACTCGCCGCTCAAGGATACCTTGCGCCATCACGCC
GCGGCGATGTGCTGTCTGGGCGCATTCGTGTCACTGACGGCGTTGTCGTTCTACATGTTCACCACCTACTTCGCGACCTA
CCTGCAAGTGGCCGGTGGACTGAGCAGGGCAACGGCGCTGCTGGTCTCGTTGATCGCGCTGGCCTTCGCCGCCGCCCTCT
GCCCACTGGCCGGTGCCTATTCGGATCGGGTAGGGCGCCGGGTCACCATGGCAACGGCCTGCATTCTGTTGATGGTCGTG
GTGGTTCCATCATTCCTGATGGCCAGCTCCGGCTCGTTCATCGCCTCGATCATCGGCGTCATGCTGCTCGCCGTGGGCGC
AGTGCTGTGTGGCGTGGTAACAGCCGCACTGCTGTCGGAAACCTTTCCCACCCGTACCCGCTACACCGCCTCGGCGATCA
CCTACAACATGGCCTACACCCTGTTCGGCGGCACCGCGCCGCTGATGGCAACCTGGCTGATCAGCATGACCGGCAGCAAC
CTGTCGCCCGCGTTCTACCTCATCGCCGTGGCAGCACTGGCAATGGCCGGCGGCATGGCCCTGCCGGAAACCTCGAAAAT
ATCGTTGCATGATGTACCTGTTACAGGCGAGGAACCGGTGGTGGGGTCAATTGCCTGA

Upstream 100 bases:

>100_bases
GACGTCGGCCCTGACTACGTGGATGACACTTCTGGAGAGGGGCATCCGGGTCGCTGTCTCGATAATCGATCCATTCCAGA
TCAACACGCCGAGGCCCGCC

Downstream 100 bases:

>100_bases
GGGCAGAGGCCGGAAACGAACATGGCTCGCATCGCAGAGCGCTGTGTGACGCAGAGCGTCACGAACTGCATTCCCACGCG
GAGCGTTGGAACGATAGTCA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTTTTVEQVDPATLKKVIVAAAIGNFVEWFDFAVYGFLATTIALQFFPSGDSSAALLKTFAVFAVAFAFRPLGGIFFGML
GDRIGRKKTLAMTILLMAGATTLIGLLPTYAAIGVMAPVLLTIIRCAQGFSAGGEYAGACAYLMEHAPRTQRAWYGSFVP
VSTFSAFAAAAVVAYALESLLSTEAMGNWGWRLPFLIAAPLGLVGLYLRWKLDETPAFQAVAQEHAVAHSPLKDTLRHHA
AAMCCLGAFVSLTALSFYMFTTYFATYLQVAGGLSRATALLVSLIALAFAAALCPLAGAYSDRVGRRVTMATACILLMVV
VVPSFLMASSGSFIASIIGVMLLAVGAVLCGVVTAALLSETFPTRTRYTASAITYNMAYTLFGGTAPLMATWLISMTGSN
LSPAFYLIAVAALAMAGGMALPETSKISLHDVPVTGEEPVVGSIA

Sequences:

>Translated_445_residues
MTTTTVEQVDPATLKKVIVAAAIGNFVEWFDFAVYGFLATTIALQFFPSGDSSAALLKTFAVFAVAFAFRPLGGIFFGML
GDRIGRKKTLAMTILLMAGATTLIGLLPTYAAIGVMAPVLLTIIRCAQGFSAGGEYAGACAYLMEHAPRTQRAWYGSFVP
VSTFSAFAAAAVVAYALESLLSTEAMGNWGWRLPFLIAAPLGLVGLYLRWKLDETPAFQAVAQEHAVAHSPLKDTLRHHA
AAMCCLGAFVSLTALSFYMFTTYFATYLQVAGGLSRATALLVSLIALAFAAALCPLAGAYSDRVGRRVTMATACILLMVV
VVPSFLMASSGSFIASIIGVMLLAVGAVLCGVVTAALLSETFPTRTRYTASAITYNMAYTLFGGTAPLMATWLISMTGSN
LSPAFYLIAVAALAMAGGMALPETSKISLHDVPVTGEEPVVGSIA
>Mature_444_residues
TTTTVEQVDPATLKKVIVAAAIGNFVEWFDFAVYGFLATTIALQFFPSGDSSAALLKTFAVFAVAFAFRPLGGIFFGMLG
DRIGRKKTLAMTILLMAGATTLIGLLPTYAAIGVMAPVLLTIIRCAQGFSAGGEYAGACAYLMEHAPRTQRAWYGSFVPV
STFSAFAAAAVVAYALESLLSTEAMGNWGWRLPFLIAAPLGLVGLYLRWKLDETPAFQAVAQEHAVAHSPLKDTLRHHAA
AMCCLGAFVSLTALSFYMFTTYFATYLQVAGGLSRATALLVSLIALAFAAALCPLAGAYSDRVGRRVTMATACILLMVVV
VPSFLMASSGSFIASIIGVMLLAVGAVLCGVVTAALLSETFPTRTRYTASAITYNMAYTLFGGTAPLMATWLISMTGSNL
SPAFYLIAVAALAMAGGMALPETSKISLHDVPVTGEEPVVGSIA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=454, Percent_Identity=36.3436123348018, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789941, Length=440, Percent_Identity=36.5909090909091, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788292, Length=402, Percent_Identity=33.0845771144279, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788942, Length=410, Percent_Identity=35.3658536585366, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87082404, Length=429, Percent_Identity=25.1748251748252, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082231, Length=214, Percent_Identity=27.5700934579439, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46695; Mature: 46563

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TTTTVEQVDPATLKKVIVAAAIGNFVEWFDFAVYGFLATTIALQFFPSGDSSAALLKTF
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH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CCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]