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The map label for this gene is dapE [H]

Identifier: 66044579

GI number: 66044579

Start: 1508611

End: 1509762

Strand: Reverse

Name: dapE [H]

Synonym: Psyr_1331

Alternate gene names: 66044579

Gene position: 1509762-1508611 (Counterclockwise)

Preceding gene: 66044580

Following gene: 66044578

Centisome position: 24.78

GC content: 64.24

Gene sequence:

>1152_bases
ATGACGGCCCCAGCCGACCTCTCGCCCACCCTCCAACTCGCCTGCGATCTGATCCGCCGTCCGTCAGTGACGCCGGTCGA
TGCCGATTGCCAGACCGTGATGATGCAGCGCCTGGGCGATGCCGGCTTCAAGCTGGAGCCAATGCGTATCGAAGACGTCG
ACAACTTCTGGGCCACCCACGGCACCACCGACGGCCCGGTACTGTGTTTCGCCGGGCATACCGACGTGGTGCCGACCGGG
CCGCTGCAGAACTGGCAGAACGACCCGTTCGATGCGTTGATCGACGAGCACGGCATGCTCTGCGGACGCGGCGCGGCAGA
CATGAAAGGCAGTCTGGCGGCCATGCTGGTGGCGGCCGAACGCTTTGTCGCCGACCACCCTGATCACAAGGGCTCGGTAG
CGTTCCTGATCACCAGCGACGAAGAAGGCCCGGCGCATCACGGCACCAAAGCCGTGGTCGAACGCCTCGCAGCGCGTAAC
GAGCGTCTGGACTGGTGCATCGTCGGCGAGCCGTCGAGCACCACGCTGGTCGGCGATGTGGTCAAGAACGGTCGGCGCGG
TTCGCTGGGGGCCACGCTGACAGTACGCGGCAAGCAGGGCCATGTGGCTTACCCGCATCTGGCGAAGAATCCGATTCACC
TGGCTGCGCCGGCCCTGGCCGAACTGGCCGCCGAGCACTGGGACCACGGCAACGACTTCTTCCCGCCGACCAGCTTCCAG
ATCTCCAACCTGAACGCTGGCACCGGCGCCACCAATGTCATCCCCGGCGATCTGGTGGCGGTCTTCAATTTCCGTTTTTC
CACCGAGTCGACCGTCGAAGGTCTGCAGCAGCGCGTTGCCGATATCCTCGACAGGCATGAACTGGACTGGCACGTGGACT
GGGCATTGTCCGGCCTGCCGTTCCTCACCGAACCGGGCGCGTTGCTGGACGCCGTGTCTTCCAGCATCAAAAGCGTCACC
GGCCGTGAGACTAAAGCCTCGACCAGCGGCGGAACCTCCGACGGTCGTTTCATCGCCACCCTCGGCACCCAAGTGGTCGA
GCTGGGGCCGGTCAACGCAACGATCCATCAGGTCAACGAACGTATTCTGGCCAGCGACCTCGATGTGCTGACCGAGATCT
ACTACCAGACTCTGGTCAAGTTGCTCGCCTGA

Upstream 100 bases:

>100_bases
ACCTATCCACCGGGCACGATAAACGACGGCCTCAGGTCGTCGTTTTGCTTTAAGATATCCGCCTTTTCGCGCCCAAAGTC
TTGCTACCGGAGTTCTGTAC

Downstream 100 bases:

>100_bases
TGCTGACCTGCCCGATCTGCTCAGCGCCGCTCAGCACTGCCGACAACGGCGTGGTGTGCCCGGCCAATCATCGTTTCGAC
CGAGCCCGTCAGGGTTATCT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTAPADLSPTLQLACDLIRRPSVTPVDADCQTVMMQRLGDAGFKLEPMRIEDVDNFWATHGTTDGPVLCFAGHTDVVPTG
PLQNWQNDPFDALIDEHGMLCGRGAADMKGSLAAMLVAAERFVADHPDHKGSVAFLITSDEEGPAHHGTKAVVERLAARN
ERLDWCIVGEPSSTTLVGDVVKNGRRGSLGATLTVRGKQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQ
ISNLNAGTGATNVIPGDLVAVFNFRFSTESTVEGLQQRVADILDRHELDWHVDWALSGLPFLTEPGALLDAVSSSIKSVT
GRETKASTSGGTSDGRFIATLGTQVVELGPVNATIHQVNERILASDLDVLTEIYYQTLVKLLA

Sequences:

>Translated_383_residues
MTAPADLSPTLQLACDLIRRPSVTPVDADCQTVMMQRLGDAGFKLEPMRIEDVDNFWATHGTTDGPVLCFAGHTDVVPTG
PLQNWQNDPFDALIDEHGMLCGRGAADMKGSLAAMLVAAERFVADHPDHKGSVAFLITSDEEGPAHHGTKAVVERLAARN
ERLDWCIVGEPSSTTLVGDVVKNGRRGSLGATLTVRGKQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQ
ISNLNAGTGATNVIPGDLVAVFNFRFSTESTVEGLQQRVADILDRHELDWHVDWALSGLPFLTEPGALLDAVSSSIKSVT
GRETKASTSGGTSDGRFIATLGTQVVELGPVNATIHQVNERILASDLDVLTEIYYQTLVKLLA
>Mature_382_residues
TAPADLSPTLQLACDLIRRPSVTPVDADCQTVMMQRLGDAGFKLEPMRIEDVDNFWATHGTTDGPVLCFAGHTDVVPTGP
LQNWQNDPFDALIDEHGMLCGRGAADMKGSLAAMLVAAERFVADHPDHKGSVAFLITSDEEGPAHHGTKAVVERLAARNE
RLDWCIVGEPSSTTLVGDVVKNGRRGSLGATLTVRGKQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQI
SNLNAGTGATNVIPGDLVAVFNFRFSTESTVEGLQQRVADILDRHELDWHVDWALSGLPFLTEPGALLDAVSSSIKSVTG
RETKASTSGGTSDGRFIATLGTQVVELGPVNATIHQVNERILASDLDVLTEIYYQTLVKLLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=59.4666666666667, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1790395, Length=226, Percent_Identity=30.5309734513274, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41063; Mature: 40931

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPADLSPTLQLACDLIRRPSVTPVDADCQTVMMQRLGDAGFKLEPMRIEDVDNFWATH
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEECCEECCCCHHHHHCC
GTTDGPVLCFAGHTDVVPTGPLQNWQNDPFDALIDEHGMLCGRGAADMKGSLAAMLVAAE
CCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCCCHHHHHHHHHHHHH
RFVADHPDHKGSVAFLITSDEEGPAHHGTKAVVERLAARNERLDWCIVGEPSSTTLVGDV
HHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCEEHHH
VKNGRRGSLGATLTVRGKQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQ
HHCCCCCCCCEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEE
ISNLNAGTGATNVIPGDLVAVFNFRFSTESTVEGLQQRVADILDRHELDWHVDWALSGLP
EECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCC
FLTEPGALLDAVSSSIKSVTGRETKASTSGGTSDGRFIATLGTQVVELGPVNATIHQVNE
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHEEECCCCHHHHHHHH
RILASDLDVLTEIYYQTLVKLLA
HHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TAPADLSPTLQLACDLIRRPSVTPVDADCQTVMMQRLGDAGFKLEPMRIEDVDNFWATH
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEECCEECCCCHHHHHCC
GTTDGPVLCFAGHTDVVPTGPLQNWQNDPFDALIDEHGMLCGRGAADMKGSLAAMLVAAE
CCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCCCHHHHHHHHHHHHH
RFVADHPDHKGSVAFLITSDEEGPAHHGTKAVVERLAARNERLDWCIVGEPSSTTLVGDV
HHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCEEHHH
VKNGRRGSLGATLTVRGKQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQ
HHCCCCCCCCEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEE
ISNLNAGTGATNVIPGDLVAVFNFRFSTESTVEGLQQRVADILDRHELDWHVDWALSGLP
EECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCC
FLTEPGALLDAVSSSIKSVTGRETKASTSGGTSDGRFIATLGTQVVELGPVNATIHQVNE
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHEEECCCCHHHHHHHH
RILASDLDVLTEIYYQTLVKLLA
HHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA