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The map label for this gene is rlmA [H]

Identifier: 66044578

GI number: 66044578

Start: 1507802

End: 1508611

Strand: Reverse

Name: rlmA [H]

Synonym: Psyr_1330

Alternate gene names: 66044578

Gene position: 1508611-1507802 (Counterclockwise)

Preceding gene: 66044579

Following gene: 66044577

Centisome position: 24.76

GC content: 63.95

Gene sequence:

>810_bases
ATGCTGACCTGCCCGATCTGCTCAGCGCCGCTCAGCACTGCCGACAACGGCGTGGTGTGCCCGGCCAATCATCGTTTCGA
CCGAGCCCGTCAGGGTTATCTGAACCTGTTGCCGGTGCAGCACAAGAACAGCCGCGACCCGGGCGACAATCAGGCCATGG
TCGAGGCGCGACGCGATTTTCTCAACGCCGGGCACTACGCGCCGGTAGCCCGGCGACTGGCTGAACTGGCCGCCGAGCGA
TCCCCCGCGCATTGGCTGGACATCGGTTGTGGCGAAGGTTATTACACCGCGCAGATTGCCGAAGCCTTGCCGCATGCCGA
TGGCTATGCGCTGGACATCTCCCGCGAGGCGGTCAAACGCGCCTGTCGTCGTGATCCGGCGCTGACCTGGATGGTCGCCA
GCATGGCCCGTGTGCCACTTGCGGATGCCAGCTGCCAGTTTCTGGCCAGCGTGTTCAGCCCGCTGGACTGGCAGGAAGCC
AAGCGCTTGCTGACCCCAGGCGGCGGGCTGATGCGGGTCGGCCCGACCAATGAGCATCTGATGGAGCTGCGTCAGCGGCT
CTATGATGAAGTGCGCGATTACGCCGACGACAAGCACCTGTCGCTGGTGCCGCAAGGCATGCAGTTGCAACACAGCGAAA
CCCTGCGCTACACGCTGAAGCTGATCGACGGCCAGTCCCGCGCCGATCTGCTGGCCATGACACCGCACGGCTGGCGCGCC
AGCGCCGAACGTCGGGCAGCGGTCATCGAGCAACCAGGACCTTTCAAGGTCACCGTTTCCATGCGCTACGATTATTTCGT
GCTGCAATAA

Upstream 100 bases:

>100_bases
TCAACGCAACGATCCATCAGGTCAACGAACGTATTCTGGCCAGCGACCTCGATGTGCTGACCGAGATCTACTACCAGACT
CTGGTCAAGTTGCTCGCCTG

Downstream 100 bases:

>100_bases
GCATTCAACAGAGGCCCCCATGCGCCAACCCGATATCGAGATTTACCTGAAAGACGAAGACGTCGATCACAAGGCAATCG
CCCAGTGGCTCGGCAAAGCG

Product: rRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MLTCPICSAPLSTADNGVVCPANHRFDRARQGYLNLLPVQHKNSRDPGDNQAMVEARRDFLNAGHYAPVARRLAELAAER
SPAHWLDIGCGEGYYTAQIAEALPHADGYALDISREAVKRACRRDPALTWMVASMARVPLADASCQFLASVFSPLDWQEA
KRLLTPGGGLMRVGPTNEHLMELRQRLYDEVRDYADDKHLSLVPQGMQLQHSETLRYTLKLIDGQSRADLLAMTPHGWRA
SAERRAAVIEQPGPFKVTVSMRYDYFVLQ

Sequences:

>Translated_269_residues
MLTCPICSAPLSTADNGVVCPANHRFDRARQGYLNLLPVQHKNSRDPGDNQAMVEARRDFLNAGHYAPVARRLAELAAER
SPAHWLDIGCGEGYYTAQIAEALPHADGYALDISREAVKRACRRDPALTWMVASMARVPLADASCQFLASVFSPLDWQEA
KRLLTPGGGLMRVGPTNEHLMELRQRLYDEVRDYADDKHLSLVPQGMQLQHSETLRYTLKLIDGQSRADLLAMTPHGWRA
SAERRAAVIEQPGPFKVTVSMRYDYFVLQ
>Mature_269_residues
MLTCPICSAPLSTADNGVVCPANHRFDRARQGYLNLLPVQHKNSRDPGDNQAMVEARRDFLNAGHYAPVARRLAELAAER
SPAHWLDIGCGEGYYTAQIAEALPHADGYALDISREAVKRACRRDPALTWMVASMARVPLADASCQFLASVFSPLDWQEA
KRLLTPGGGLMRVGPTNEHLMELRQRLYDEVRDYADDKHLSLVPQGMQLQHSETLRYTLKLIDGQSRADLLAMTPHGWRA
SAERRAAVIEQPGPFKVTVSMRYDYFVLQ

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=246, Percent_Identity=43.9024390243902, Blast_Score=204, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 30071; Mature: 30071

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTCPICSAPLSTADNGVVCPANHRFDRARQGYLNLLPVQHKNSRDPGDNQAMVEARRDF
CCCCCCCCCCCCCCCCCEECCCCCCHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
LNAGHYAPVARRLAELAAERSPAHWLDIGCGEGYYTAQIAEALPHADGYALDISREAVKR
HCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCCCCEEEECCHHHHHH
ACRRDPALTWMVASMARVPLADASCQFLASVFSPLDWQEAKRLLTPGGGLMRVGPTNEHL
HHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEECCCHHHH
MELRQRLYDEVRDYADDKHLSLVPQGMQLQHSETLRYTLKLIDGQSRADLLAMTPHGWRA
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHEEEEHHHCCCCCCCEEEECCCCCCC
SAERRAAVIEQPGPFKVTVSMRYDYFVLQ
CHHHHHHEECCCCCEEEEEEEEEEEEEEC
>Mature Secondary Structure
MLTCPICSAPLSTADNGVVCPANHRFDRARQGYLNLLPVQHKNSRDPGDNQAMVEARRDF
CCCCCCCCCCCCCCCCCEECCCCCCHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
LNAGHYAPVARRLAELAAERSPAHWLDIGCGEGYYTAQIAEALPHADGYALDISREAVKR
HCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCCCCEEEECCHHHHHH
ACRRDPALTWMVASMARVPLADASCQFLASVFSPLDWQEAKRLLTPGGGLMRVGPTNEHL
HHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEECCCHHHH
MELRQRLYDEVRDYADDKHLSLVPQGMQLQHSETLRYTLKLIDGQSRADLLAMTPHGWRA
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHEEEEHHHCCCCCCCEEEECCCCCCC
SAERRAAVIEQPGPFKVTVSMRYDYFVLQ
CHHHHHHEECCCCCEEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]