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The map label for this gene is cusS [H]

Identifier: 66044377

GI number: 66044377

Start: 1274985

End: 1276349

Strand: Reverse

Name: cusS [H]

Synonym: Psyr_1126

Alternate gene names: 66044377

Gene position: 1276349-1274985 (Counterclockwise)

Preceding gene: 66044378

Following gene: 66044376

Centisome position: 20.95

GC content: 61.25

Gene sequence:

>1365_bases
GTGCGGGTTGATTCGATTGCTTTACGCCTGAGCGGGATGTTCGCGCTGGTGGCACTGGTGGTGTTCCTGCTGATCGGCTG
GGCCTTGTACCTGCAGGTCGACAAGAGCCTCGACCTGCTGCCCCGCGCGGAAGTGGATGCGCGCTACAGCGTGCTGGAAT
CGGCGGTCAATCGTTTCGGCAACCCGGAGCACTGGGGCAAGATCAAGAACAAGCTCAGTTTGCTCAGCGAAGAGGACAAG
CGCCTGCGCTTCTGGGTGGTGAGTGACAATCCGCTCTACGAGTTCGGCAATCCCGGTGCGGACATTCGTCGTTTTGCGCA
CGGGCCGCTGGGCATGCACGACCTGAGACTGGCAGATCATCCGTTTTCGTTCAAAGTGCTGGTCAGCGAGTTTCCCGCCA
AGGAACAGCGTCCGGCACTGCGCTTTCTGACCGCCATCGACACCGAGACGTTCTGGCACACGCAACACTCGCTGCTGGTC
GCCCTGATCAGCCTGGCCACGCTGGGCATTCTGCTCGCGTCGGCGCTGGGCTACTGGGTCGCGCGAATTGGCCTGCGACC
GCTGACCAGCCTCTCGCAGGAAGTCCAGAAGCTCGCCCCGCCGCGGCTGTCCGGACGCTTGCAGCTGTCGCCGCTGCCGC
CGGAACTCGAACAATTCGTCGCCTCATTCAACTCCACGCTTGAACGCGTCGAGCAGGCCTACTCGAGGCTGGAGTCGTTC
AATGCCGATGTGGCGCACGAGCTTCGCTCACCGCTGACCAATCTGATCGGCCAGACCCAGGTCGCGCTGACCCGCGGCCG
CTCTGCCGAACATTATTTCGAGGTGCTGCAGTCGAATCTCGAAGAGCTGGAGCGCCTGCGCTCGATCATCAACGACATGC
TGTTTCTGGCCAGCGCCGATCAGGGCACCAGGGTCAAGGCACAGACCTGCACCTCACTGGCTGAAGAAGTCGCTACGACG
CTGGATTACCTGGACTTCATTCTGGAAGACGCGCACGTGCAAGTCCGTGTCAACGGCGACGCCAGCGCGCCGATTGAAAA
ATCCCACTTGCGCCGAGCGCTGATCAACCTGTTGCATAACGCCGTCCAGCACACCGGCGCTGGCGAAGTCATTGAAGTCA
ACATCGAATCGCGCGATGCCCATGTCACTGTCAGCGTGACCAATCCGGGCGTACACATCGCTCAGGAACATTTACCCAGA
CTGTTCGAGCGCTTTTATCGGGTGGATGCCTCACGCAGCAACAGCGGCGCCAATCACGGCCTGGGGCTGGCCATCGTCAA
GGCGATTGCCTTGATGCATGGCGGCACCGTGTTCGTGAACAGCGACAAGGGCGCCAATACTTTCGGCATCCAGTTGCCCC
GCTGA

Upstream 100 bases:

>100_bases
GAAGTCGCGATCAAGCGCTTGCGGGCCAAGCTCGATGGCCCTCACGAGCACAAGCTGCTGCACACCATTCGCGGCATGGG
CTACGTGCTGGAGAACCGCA

Downstream 100 bases:

>100_bases
CACCTGAAAATACTGACTGCACTGACGGTTTTCACGGTCCTCAGTGTGCGCCTGTTCGCAGCATTTTAAATACACTTGCT
TGATTCACATTCCCTTCATA

Product: heavy metal sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MRVDSIALRLSGMFALVALVVFLLIGWALYLQVDKSLDLLPRAEVDARYSVLESAVNRFGNPEHWGKIKNKLSLLSEEDK
RLRFWVVSDNPLYEFGNPGADIRRFAHGPLGMHDLRLADHPFSFKVLVSEFPAKEQRPALRFLTAIDTETFWHTQHSLLV
ALISLATLGILLASALGYWVARIGLRPLTSLSQEVQKLAPPRLSGRLQLSPLPPELEQFVASFNSTLERVEQAYSRLESF
NADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASADQGTRVKAQTCTSLAEEVATT
LDYLDFILEDAHVQVRVNGDASAPIEKSHLRRALINLLHNAVQHTGAGEVIEVNIESRDAHVTVSVTNPGVHIAQEHLPR
LFERFYRVDASRSNSGANHGLGLAIVKAIALMHGGTVFVNSDKGANTFGIQLPR

Sequences:

>Translated_454_residues
MRVDSIALRLSGMFALVALVVFLLIGWALYLQVDKSLDLLPRAEVDARYSVLESAVNRFGNPEHWGKIKNKLSLLSEEDK
RLRFWVVSDNPLYEFGNPGADIRRFAHGPLGMHDLRLADHPFSFKVLVSEFPAKEQRPALRFLTAIDTETFWHTQHSLLV
ALISLATLGILLASALGYWVARIGLRPLTSLSQEVQKLAPPRLSGRLQLSPLPPELEQFVASFNSTLERVEQAYSRLESF
NADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASADQGTRVKAQTCTSLAEEVATT
LDYLDFILEDAHVQVRVNGDASAPIEKSHLRRALINLLHNAVQHTGAGEVIEVNIESRDAHVTVSVTNPGVHIAQEHLPR
LFERFYRVDASRSNSGANHGLGLAIVKAIALMHGGTVFVNSDKGANTFGIQLPR
>Mature_454_residues
MRVDSIALRLSGMFALVALVVFLLIGWALYLQVDKSLDLLPRAEVDARYSVLESAVNRFGNPEHWGKIKNKLSLLSEEDK
RLRFWVVSDNPLYEFGNPGADIRRFAHGPLGMHDLRLADHPFSFKVLVSEFPAKEQRPALRFLTAIDTETFWHTQHSLLV
ALISLATLGILLASALGYWVARIGLRPLTSLSQEVQKLAPPRLSGRLQLSPLPPELEQFVASFNSTLERVEQAYSRLESF
NADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASADQGTRVKAQTCTSLAEEVATT
LDYLDFILEDAHVQVRVNGDASAPIEKSHLRRALINLLHNAVQHTGAGEVIEVNIESRDAHVTVSVTNPGVHIAQEHLPR
LFERFYRVDASRSNSGANHGLGLAIVKAIALMHGGTVFVNSDKGANTFGIQLPR

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=366, Percent_Identity=39.0710382513661, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1788279, Length=293, Percent_Identity=32.4232081911263, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=32.8888888888889, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789403, Length=462, Percent_Identity=25.1082251082251, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788393, Length=272, Percent_Identity=26.4705882352941, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790346, Length=257, Percent_Identity=30.3501945525292, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790436, Length=324, Percent_Identity=27.4691358024691, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787894, Length=396, Percent_Identity=24.2424242424242, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=29.2682926829268, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787374, Length=470, Percent_Identity=23.4042553191489, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=26.0162601626016, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=26.0330578512397, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790861, Length=308, Percent_Identity=25, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1790551, Length=302, Percent_Identity=25.4966887417219, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1789149, Length=257, Percent_Identity=26.0700389105058, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789808, Length=301, Percent_Identity=26.2458471760797, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50476; Mature: 50476

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDSIALRLSGMFALVALVVFLLIGWALYLQVDKSLDLLPRAEVDARYSVLESAVNRFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
NPEHWGKIKNKLSLLSEEDKRLRFWVVSDNPLYEFGNPGADIRRFAHGPLGMHDLRLADH
CCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEECCC
PFSFKVLVSEFPAKEQRPALRFLTAIDTETFWHTQHSLLVALISLATLGILLASALGYWV
CCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARIGLRPLTSLSQEVQKLAPPRLSGRLQLSPLPPELEQFVASFNSTLERVEQAYSRLESF
HHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASAD
CHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGTRVKAQTCTSLAEEVATTLDYLDFILEDAHVQVRVNGDASAPIEKSHLRRALINLLHN
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
AVQHTGAGEVIEVNIESRDAHVTVSVTNPGVHIAQEHLPRLFERFYRVDASRSNSGANHG
HHHHCCCCCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LGLAIVKAIALMHGGTVFVNSDKGANTFGIQLPR
HHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCC
>Mature Secondary Structure
MRVDSIALRLSGMFALVALVVFLLIGWALYLQVDKSLDLLPRAEVDARYSVLESAVNRFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
NPEHWGKIKNKLSLLSEEDKRLRFWVVSDNPLYEFGNPGADIRRFAHGPLGMHDLRLADH
CCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEECCC
PFSFKVLVSEFPAKEQRPALRFLTAIDTETFWHTQHSLLVALISLATLGILLASALGYWV
CCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARIGLRPLTSLSQEVQKLAPPRLSGRLQLSPLPPELEQFVASFNSTLERVEQAYSRLESF
HHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASAD
CHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGTRVKAQTCTSLAEEVATTLDYLDFILEDAHVQVRVNGDASAPIEKSHLRRALINLLHN
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
AVQHTGAGEVIEVNIESRDAHVTVSVTNPGVHIAQEHLPRLFERFYRVDASRSNSGANHG
HHHHCCCCCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LGLAIVKAIALMHGGTVFVNSDKGANTFGIQLPR
HHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]