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The map label for this gene is ywbI [H]

Identifier: 66044164

GI number: 66044164

Start: 1030676

End: 1031572

Strand: Direct

Name: ywbI [H]

Synonym: Psyr_0909

Alternate gene names: 66044164

Gene position: 1030676-1031572 (Clockwise)

Preceding gene: 66044161

Following gene: 66044165

Centisome position: 16.91

GC content: 59.64

Gene sequence:

>897_bases
GTGAACCTTCGAACATTACGCACTTTTGTCGAAGTGGTACGTCAGGGTAGCTTCTCCCAGGCCGCTGACGTGGTGTCGCT
TACCCAGTCCTCCGTCAGCAAAGCCGTCAAGACCCTTGAGGACGAATTGGGCACGCCGCTGTTGAGTCGCATTGGCCACC
GCAGCGAACTGACCGCCGCCGGCGAAATTGTCTACCGCCGCGCCCTGACCCTGTTGGCCGAACGCAACGATCTGCTCACC
GAGCTCAACGACCTGCGTGGTTTGAAGGGCGGCGTGCTGCGCATCGGTCTGCCACCCGTCGGCAGTGGTGCGCTTTTCGC
CGCGATGTTCACTCAATACCGCGCCCGCTATCCGGATATCGATATTCAGCTCACCGAACACGGCGCGAGCAAACTGCATG
AGTGCCTGGCTTCGGGAGAAGTCGAGCTGGCAGCACTGTTGAAGATGAACTCGTTCAGCGAAGAGTTCGATTACCAGGAT
GTGCGTATCGAGCCAGTGGTGGTGGTCATGGCAGAGAACCACCCGTTGGCCAAGCGCAAACGGGTCGTCATTGAAGACCT
GGCCGAAACGCCGTTTATCCTCTTCGAGGCCGGATTTGCACTGAACACCATGATCAAACTGGCCTGCGACCGCAAGGGCG
TGACGCCCAAAGTGACTGCACGCAGCGGTCAGGTCGACTTTATTGTGGATCTGGTCTCGGCAGGGCTGGGTGTCGCGTTC
TTGCCGAGGATGCTGGCGCAGAAGCACTATCACGACGGGATTCGACTGGTTGCGCTGAACGAGCCGGATACCGATTGGCA
TATGGTGTTGGCATGGCGCAAAAACGCGCACTTGTCACCTGCGGCCGAGGCCTGGTTGGCGCTTGCCAGGGAAACCCGTC
TCCCGACGACGTCCTGA

Upstream 100 bases:

>100_bases
GCGAGGCCGCCAAGGGAGAGCGGCAGGTTCAGATTACGGAAAAACCGACTATGATCAAAACAACTAATACAACTTGAATT
CAGTCGGAAATCGAATATTT

Downstream 100 bases:

>100_bases
AATCCCGGAGGCGTGCTTTTAGCACACGCAGGGGGGGGCTGGGAGGCATAAGAGGTGTGACAAAACGGGAAGGCCAGAAA
CAAGAAAGCCCGCACGAGGC

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MNLRTLRTFVEVVRQGSFSQAADVVSLTQSSVSKAVKTLEDELGTPLLSRIGHRSELTAAGEIVYRRALTLLAERNDLLT
ELNDLRGLKGGVLRIGLPPVGSGALFAAMFTQYRARYPDIDIQLTEHGASKLHECLASGEVELAALLKMNSFSEEFDYQD
VRIEPVVVVMAENHPLAKRKRVVIEDLAETPFILFEAGFALNTMIKLACDRKGVTPKVTARSGQVDFIVDLVSAGLGVAF
LPRMLAQKHYHDGIRLVALNEPDTDWHMVLAWRKNAHLSPAAEAWLALARETRLPTTS

Sequences:

>Translated_298_residues
MNLRTLRTFVEVVRQGSFSQAADVVSLTQSSVSKAVKTLEDELGTPLLSRIGHRSELTAAGEIVYRRALTLLAERNDLLT
ELNDLRGLKGGVLRIGLPPVGSGALFAAMFTQYRARYPDIDIQLTEHGASKLHECLASGEVELAALLKMNSFSEEFDYQD
VRIEPVVVVMAENHPLAKRKRVVIEDLAETPFILFEAGFALNTMIKLACDRKGVTPKVTARSGQVDFIVDLVSAGLGVAF
LPRMLAQKHYHDGIRLVALNEPDTDWHMVLAWRKNAHLSPAAEAWLALARETRLPTTS
>Mature_298_residues
MNLRTLRTFVEVVRQGSFSQAADVVSLTQSSVSKAVKTLEDELGTPLLSRIGHRSELTAAGEIVYRRALTLLAERNDLLT
ELNDLRGLKGGVLRIGLPPVGSGALFAAMFTQYRARYPDIDIQLTEHGASKLHECLASGEVELAALLKMNSFSEEFDYQD
VRIEPVVVVMAENHPLAKRKRVVIEDLAETPFILFEAGFALNTMIKLACDRKGVTPKVTARSGQVDFIVDLVSAGLGVAF
LPRMLAQKHYHDGIRLVALNEPDTDWHMVLAWRKNAHLSPAAEAWLALARETRLPTTS

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=286, Percent_Identity=29.020979020979, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787879, Length=287, Percent_Identity=30.3135888501742, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788887, Length=300, Percent_Identity=29, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790399, Length=295, Percent_Identity=27.4576271186441, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787530, Length=251, Percent_Identity=26.6932270916335, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693105, Length=296, Percent_Identity=23.6486486486486, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788481, Length=299, Percent_Identity=27.7591973244147, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788748, Length=290, Percent_Identity=26.8965517241379, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788297, Length=243, Percent_Identity=26.3374485596708, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789506, Length=289, Percent_Identity=21.1072664359862, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=26.6932270916335, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787128, Length=125, Percent_Identity=33.6, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788296, Length=251, Percent_Identity=25.0996015936255, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693193, Length=133, Percent_Identity=33.0827067669173, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081904, Length=201, Percent_Identity=25.3731343283582, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI2367136, Length=251, Percent_Identity=22.3107569721116, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787601, Length=284, Percent_Identity=20.7746478873239, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=296, Percent_Identity=22.972972972973, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786401, Length=119, Percent_Identity=36.1344537815126, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786713, Length=283, Percent_Identity=26.8551236749117, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=32.7868852459016, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789204, Length=254, Percent_Identity=20.4724409448819, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789440, Length=121, Percent_Identity=29.7520661157025, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32883; Mature: 32883

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRTLRTFVEVVRQGSFSQAADVVSLTQSSVSKAVKTLEDELGTPLLSRIGHRSELTAA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
GEIVYRRALTLLAERNDLLTELNDLRGLKGGVLRIGLPPVGSGALFAAMFTQYRARYPDI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
DIQLTEHGASKLHECLASGEVELAALLKMNSFSEEFDYQDVRIEPVVVVMAENHPLAKRK
EEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCCCCEEECEEEEEEECCCCHHHHH
RVVIEDLAETPFILFEAGFALNTMIKLACDRKGVTPKVTARSGQVDFIVDLVSAGLGVAF
HHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHCCCHHHH
LPRMLAQKHYHDGIRLVALNEPDTDWHMVLAWRKNAHLSPAAEAWLALARETRLPTTS
HHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNLRTLRTFVEVVRQGSFSQAADVVSLTQSSVSKAVKTLEDELGTPLLSRIGHRSELTAA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
GEIVYRRALTLLAERNDLLTELNDLRGLKGGVLRIGLPPVGSGALFAAMFTQYRARYPDI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
DIQLTEHGASKLHECLASGEVELAALLKMNSFSEEFDYQDVRIEPVVVVMAENHPLAKRK
EEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCCCCEEECEEEEEEECCCCHHHHH
RVVIEDLAETPFILFEAGFALNTMIKLACDRKGVTPKVTARSGQVDFIVDLVSAGLGVAF
HHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHCCCHHHH
LPRMLAQKHYHDGIRLVALNEPDTDWHMVLAWRKNAHLSPAAEAWLALARETRLPTTS
HHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]