Click here to switch to the map view.

The map label for this gene is mrcA [H]

Identifier: 66043669

GI number: 66043669

Start: 430973

End: 433417

Strand: Reverse

Name: mrcA [H]

Synonym: Psyr_0402

Alternate gene names: 66043669

Gene position: 433417-430973 (Counterclockwise)

Preceding gene: 66043682

Following gene: 66043665

Centisome position: 7.11

GC content: 60.98

Gene sequence:

>2445_bases
TTGATACGCCTGCTGAAGTTTTTCTGGTGGTCCCTCGTCGCGGTATTCTGCTCGCTGTTGCTTGGCCTGAGCGGCGCGTT
TCTTTACCTGAGCCCGACCCTGCCCTCCGTTGACGCGCTGCGAAGCATTCAACTGCAAATCCCCCTGCGCGTGTTCACCA
GCGACGGCAAGCTGATTGCCGAGTTCGGTGAAATGCGCCGCACGCCGATTCGCTTCGCGGAAATCCCGCCCAATTTCATC
AACGCACTGCTGTCGGCCGAAGACGACAACTTCGCCAACCACTACGGCGTCGACCCGGGCAGCCTGATGCGCGCCGCCAC
TCAACTGGTCAAGAGCGGCCATATCCAGTCAGGCGGCAGTACCATCACCATGCAGGTCGCGAAAAACTTCTTCCTGACCA
GCGAGCGCAGCTTTTCGCGCAAAACCACCGAGATCCTGTTGGCCTTGCAAATCGAGCGCCAACTTACCAAGGACGAGATT
CTCGAGCTGTACGTCAACAAGATTTATCTGGGTAACCGCGCCTACGGGATCGAGGCAGCGGCGCAAGTCTACTATGGCAA
ATCGATTCGCGATGTAAGCCTTGCGCAAATGGCCATGATTGCCGGTCTGCCCAAGGCACCGTCACGCTTCAACCCGTTGG
CCAACCCGACCCGTGCCAAGGAACGTCGCGACTGGATCCTCGGCCGCATGTATCGTCTGGGCAAGATCGACCAGGCCGCT
TACCAGGCAGCGCTGAACGAACCGCTCAACGCCAGCTATCACGTGCCGACGCCGGAAGTGAATGCGCCGTACATCGCTGA
AATGGCGCGGGCAGAAATGGTCGGCCGCTATGGCAGCGAGGCCTACACAGAAGGCTTCCGCGTCACCACGACGGTGCCGA
GCGACCTTCAGGAACACGCCAACAAGTCCATCCAGCAAGGCTTGATCGATTATGACCAGCGCCACGGCTACCGTGGTCCG
GAAAGCCGTTTCCCGGGCATGACCCGCGCCAACTGGGTACAGGAACTGACCAAGCAGCGCATGATCAACGGCCTCGAGCC
CGGCATCGTGACCCAGGTTGACAAGACCGGTCTGCGCGTTCTGACCCACAACGGCGAGAAGGAAGAAACCGTAGACTGGG
CCACCATGAAGTGGGCGCGGCCTTACCTGAATACCAACAGCCTGGGCGCCAATCCGAAGCAGCCGGGTGACGTTGCCAAG
GTCGGCGACCTGGTTCGTCTGCTGCGCCAGGACGATGGCTCGCTGAAATTTAGTCAAATTCCCGTCGCGCAGAGCGCACT
GGTATCGCTTGATCCGCAGAACGGTGCCATTCGTGCGCTGGTCGGCGGCTTCGCGTTCGAGCAGAGCAACTACAACCGCG
CCATGCAGGCCAAACGTCAACCGGGCTCCAGCTTCAAGCCGTTCATCTACAGCGCTGCGCTGGATAACGGCTACACCGCT
TCCAGCCTGGTGAACGACGCGCCTATCGTCTTTGTCGACGAATACCTGGACAAGGTATGGCGTCCGAAAAATGACACCAA
CACCTTCCTCGGGCCGATCCGCCTGCGCGAGGCGCTGTACAAGTCTCGTAACCTGGTATCGATCCGCTTGCTTCAGGCCC
TCGGCATCGACAGCACCATCGACTACATCACCCGCTTCGGCTTCTCCAAGCAGGACTTGCCGCGCAACCTGTCGCTGGCA
CTGGGTACGGCGACACTGACCCCGATGGAAATCGCCACCGGCTGGGCGACCTTCGCCAACGGCGGTTACAAGGTCAGTCC
TTATCTGATCCAGACCATCGAGAACCGTGACGGCCAGACCCTGTTTGTCGCCAACCCGCCAAGCGTTCCGGCCAATGACG
TCAACACCGCGCCCAATACTTCGACCTTTGCGGTACATGACAGCCCGAGCGTCGTCACCGGTCCACCGGGCGCACCCGCT
CAGCCTCAGGCGCCCGCAGTGGCAGAGCGCGTGGTCGATGCCCGCACCACGTACATCCTGAACAGCATGCTGCAGGACGT
GATCAAGCGCGGCACCGGGCGCCGCGCGCTGTCCATGAACAGACCGGACATCGCCGGCAAGACCGGTACCACCAACGATT
CCAAGGATGCCTGGTTCTCCGGGTATAACGCGGACTACGTGACGACGGTGTGGAGTGGTTTCGATCAGCCTGAAAGCCTT
GGCCGTCACGAATTCGGCGGGACGGTCTCGCTGCCGATCTGGATGGACTACATGAGCGCCGCGCTCAAGGACAAGCCACC
GCATCAGCAGGCCGAACCGCCAGGCATTCTCAGCCTGCGCGTCGACCCATTGAGTGGCCGCGCCGCTACGCCAAGCACGC
CGAACGCATTCTTCGAGCTGTTCAAAAGCGAAGACACACCGCCAAGCGTCAACGAACTGGGCGGCAGCAGTGCACCGGGC
AGCCCGTTACCGGCAGATGAGTCCGCGCCAATCGATTTGTTCTGA

Upstream 100 bases:

>100_bases
TATAATGCCCAGCGTTTTTTTCATTTAACAAAGCTTCAAGGCATGTTCATTTGTAAATGCCTGCGCTTAACCCATTATTT
TTTCTGGAAATCCAAAAGCC

Downstream 100 bases:

>100_bases
TAGCACTGCGCAGGCTTGTGACGCGGAGCGTCACGAAAGGCATTGCCACGCTGGAGCGTGAGGAACGATAGTCGTCAGTC
AGATCACCATCGTGCCCATG

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MIRLLKFFWWSLVAVFCSLLLGLSGAFLYLSPTLPSVDALRSIQLQIPLRVFTSDGKLIAEFGEMRRTPIRFAEIPPNFI
NALLSAEDDNFANHYGVDPGSLMRAATQLVKSGHIQSGGSTITMQVAKNFFLTSERSFSRKTTEILLALQIERQLTKDEI
LELYVNKIYLGNRAYGIEAAAQVYYGKSIRDVSLAQMAMIAGLPKAPSRFNPLANPTRAKERRDWILGRMYRLGKIDQAA
YQAALNEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSEAYTEGFRVTTTVPSDLQEHANKSIQQGLIDYDQRHGYRGP
ESRFPGMTRANWVQELTKQRMINGLEPGIVTQVDKTGLRVLTHNGEKEETVDWATMKWARPYLNTNSLGANPKQPGDVAK
VGDLVRLLRQDDGSLKFSQIPVAQSALVSLDPQNGAIRALVGGFAFEQSNYNRAMQAKRQPGSSFKPFIYSAALDNGYTA
SSLVNDAPIVFVDEYLDKVWRPKNDTNTFLGPIRLREALYKSRNLVSIRLLQALGIDSTIDYITRFGFSKQDLPRNLSLA
LGTATLTPMEIATGWATFANGGYKVSPYLIQTIENRDGQTLFVANPPSVPANDVNTAPNTSTFAVHDSPSVVTGPPGAPA
QPQAPAVAERVVDARTTYILNSMLQDVIKRGTGRRALSMNRPDIAGKTGTTNDSKDAWFSGYNADYVTTVWSGFDQPESL
GRHEFGGTVSLPIWMDYMSAALKDKPPHQQAEPPGILSLRVDPLSGRAATPSTPNAFFELFKSEDTPPSVNELGGSSAPG
SPLPADESAPIDLF

Sequences:

>Translated_814_residues
MIRLLKFFWWSLVAVFCSLLLGLSGAFLYLSPTLPSVDALRSIQLQIPLRVFTSDGKLIAEFGEMRRTPIRFAEIPPNFI
NALLSAEDDNFANHYGVDPGSLMRAATQLVKSGHIQSGGSTITMQVAKNFFLTSERSFSRKTTEILLALQIERQLTKDEI
LELYVNKIYLGNRAYGIEAAAQVYYGKSIRDVSLAQMAMIAGLPKAPSRFNPLANPTRAKERRDWILGRMYRLGKIDQAA
YQAALNEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSEAYTEGFRVTTTVPSDLQEHANKSIQQGLIDYDQRHGYRGP
ESRFPGMTRANWVQELTKQRMINGLEPGIVTQVDKTGLRVLTHNGEKEETVDWATMKWARPYLNTNSLGANPKQPGDVAK
VGDLVRLLRQDDGSLKFSQIPVAQSALVSLDPQNGAIRALVGGFAFEQSNYNRAMQAKRQPGSSFKPFIYSAALDNGYTA
SSLVNDAPIVFVDEYLDKVWRPKNDTNTFLGPIRLREALYKSRNLVSIRLLQALGIDSTIDYITRFGFSKQDLPRNLSLA
LGTATLTPMEIATGWATFANGGYKVSPYLIQTIENRDGQTLFVANPPSVPANDVNTAPNTSTFAVHDSPSVVTGPPGAPA
QPQAPAVAERVVDARTTYILNSMLQDVIKRGTGRRALSMNRPDIAGKTGTTNDSKDAWFSGYNADYVTTVWSGFDQPESL
GRHEFGGTVSLPIWMDYMSAALKDKPPHQQAEPPGILSLRVDPLSGRAATPSTPNAFFELFKSEDTPPSVNELGGSSAPG
SPLPADESAPIDLF
>Mature_814_residues
MIRLLKFFWWSLVAVFCSLLLGLSGAFLYLSPTLPSVDALRSIQLQIPLRVFTSDGKLIAEFGEMRRTPIRFAEIPPNFI
NALLSAEDDNFANHYGVDPGSLMRAATQLVKSGHIQSGGSTITMQVAKNFFLTSERSFSRKTTEILLALQIERQLTKDEI
LELYVNKIYLGNRAYGIEAAAQVYYGKSIRDVSLAQMAMIAGLPKAPSRFNPLANPTRAKERRDWILGRMYRLGKIDQAA
YQAALNEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSEAYTEGFRVTTTVPSDLQEHANKSIQQGLIDYDQRHGYRGP
ESRFPGMTRANWVQELTKQRMINGLEPGIVTQVDKTGLRVLTHNGEKEETVDWATMKWARPYLNTNSLGANPKQPGDVAK
VGDLVRLLRQDDGSLKFSQIPVAQSALVSLDPQNGAIRALVGGFAFEQSNYNRAMQAKRQPGSSFKPFIYSAALDNGYTA
SSLVNDAPIVFVDEYLDKVWRPKNDTNTFLGPIRLREALYKSRNLVSIRLLQALGIDSTIDYITRFGFSKQDLPRNLSLA
LGTATLTPMEIATGWATFANGGYKVSPYLIQTIENRDGQTLFVANPPSVPANDVNTAPNTSTFAVHDSPSVVTGPPGAPA
QPQAPAVAERVVDARTTYILNSMLQDVIKRGTGRRALSMNRPDIAGKTGTTNDSKDAWFSGYNADYVTTVWSGFDQPESL
GRHEFGGTVSLPIWMDYMSAALKDKPPHQQAEPPGILSLRVDPLSGRAATPSTPNAFFELFKSEDTPPSVNELGGSSAPG
SPLPADESAPIDLF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=839, Percent_Identity=39.9284862932062, Blast_Score=616, Evalue=1e-177,
Organism=Escherichia coli, GI1786343, Length=324, Percent_Identity=30.8641975308642, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1788867, Length=258, Percent_Identity=34.4961240310078, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1789601, Length=161, Percent_Identity=36.0248447204969, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 89461; Mature: 89461

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLLKFFWWSLVAVFCSLLLGLSGAFLYLSPTLPSVDALRSIQLQIPLRVFTSDGKLIA
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHEEEEEEEEEEECCCHHHH
EFGEMRRTPIRFAEIPPNFINALLSAEDDNFANHYGVDPGSLMRAATQLVKSGHIQSGGS
HHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCC
TITMQVAKNFFLTSERSFSRKTTEILLALQIERQLTKDEILELYVNKIYLGNRAYGIEAA
EEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCHHHH
AQVYYGKSIRDVSLAQMAMIAGLPKAPSRFNPLANPTRAKERRDWILGRMYRLGKIDQAA
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
YQAALNEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSEAYTEGFRVTTTVPSDLQEHA
HHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCEEEEECCHHHHHHHH
NKSIQQGLIDYDQRHGYRGPESRFPGMTRANWVQELTKQRMINGLEPGIVTQVDKTGLRV
HHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEE
LTHNGEKEETVDWATMKWARPYLNTNSLGANPKQPGDVAKVGDLVRLLRQDDGSLKFSQI
EECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEC
PVAQSALVSLDPQNGAIRALVGGFAFEQSNYNRAMQAKRQPGSSFKPFIYSAALDNGYTA
CCHHHHHEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCH
SSLVNDAPIVFVDEYLDKVWRPKNDTNTFLGPIRLREALYKSRNLVSIRLLQALGIDSTI
HHHCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
DYITRFGFSKQDLPRNLSLALGTATLTPMEIATGWATFANGGYKVSPYLIQTIENRDGQT
HHHHHCCCCHHCCCCCCEEEECCCCCCHHHHHHCHHHHCCCCEEECHHHEEECCCCCCCE
LFVANPPSVPANDVNTAPNTSTFAVHDSPSVVTGPPGAPAQPQAPAVAERVVDARTTYIL
EEEECCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
NSMLQDVIKRGTGRRALSMNRPDIAGKTGTTNDSKDAWFSGYNADYVTTVWSGFDQPESL
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHC
GRHEFGGTVSLPIWMDYMSAALKDKPPHQQAEPPGILSLRVDPLSGRAATPSTPNAFFEL
CCHHCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
FKSEDTPPSVNELGGSSAPGSPLPADESAPIDLF
HHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIRLLKFFWWSLVAVFCSLLLGLSGAFLYLSPTLPSVDALRSIQLQIPLRVFTSDGKLIA
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHEEEEEEEEEEECCCHHHH
EFGEMRRTPIRFAEIPPNFINALLSAEDDNFANHYGVDPGSLMRAATQLVKSGHIQSGGS
HHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCC
TITMQVAKNFFLTSERSFSRKTTEILLALQIERQLTKDEILELYVNKIYLGNRAYGIEAA
EEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCHHHH
AQVYYGKSIRDVSLAQMAMIAGLPKAPSRFNPLANPTRAKERRDWILGRMYRLGKIDQAA
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
YQAALNEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSEAYTEGFRVTTTVPSDLQEHA
HHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCEEEEECCHHHHHHHH
NKSIQQGLIDYDQRHGYRGPESRFPGMTRANWVQELTKQRMINGLEPGIVTQVDKTGLRV
HHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEE
LTHNGEKEETVDWATMKWARPYLNTNSLGANPKQPGDVAKVGDLVRLLRQDDGSLKFSQI
EECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEC
PVAQSALVSLDPQNGAIRALVGGFAFEQSNYNRAMQAKRQPGSSFKPFIYSAALDNGYTA
CCHHHHHEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCH
SSLVNDAPIVFVDEYLDKVWRPKNDTNTFLGPIRLREALYKSRNLVSIRLLQALGIDSTI
HHHCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
DYITRFGFSKQDLPRNLSLALGTATLTPMEIATGWATFANGGYKVSPYLIQTIENRDGQT
HHHHHCCCCHHCCCCCCEEEECCCCCCHHHHHHCHHHHCCCCEEECHHHEEECCCCCCCE
LFVANPPSVPANDVNTAPNTSTFAVHDSPSVVTGPPGAPAQPQAPAVAERVVDARTTYIL
EEEECCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
NSMLQDVIKRGTGRRALSMNRPDIAGKTGTTNDSKDAWFSGYNADYVTTVWSGFDQPESL
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHC
GRHEFGGTVSLPIWMDYMSAALKDKPPHQQAEPPGILSLRVDPLSGRAATPSTPNAFFEL
CCHHCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
FKSEDTPPSVNELGGSSAPGSPLPADESAPIDLF
HHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043; 7901733 [H]