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The map label for this gene is priA [H]

Identifier: 66043665

GI number: 66043665

Start: 425511

End: 427730

Strand: Reverse

Name: priA [H]

Synonym: Psyr_0398

Alternate gene names: 66043665

Gene position: 427730-425511 (Counterclockwise)

Preceding gene: 66043669

Following gene: 66043664

Centisome position: 7.02

GC content: 65.32

Gene sequence:

>2220_bases
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCCGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGTCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTACCCGCCGACAAGCTGAAGCCGGCTATCGCCCTGCTCGACAGTGAAGCGCCGCTGCCGCCCGCA
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GCTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAAGAGCGCTTCTGGCATGTGGCCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAAGCCCTGACCACCCTTGCCCAGCACCCGCACGGAGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTTAACAGGGACAGCCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCACTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGATTCGACAGCTTTCATGCGTTTCTGCTGGCCGGTGTCACCGGCAGCGGCAAGACCGAAGTCTATCTGCAA
CTGATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAACGCTTCAATGCGCGCATTGCGCTGATCCACTCGGCGGTCAACGACCGCGAACGGCTGGACGCCTGGC
TGGCGGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGTTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCACTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTGGGCTCGGCCACGCCGTCCCTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTATGGCCTGTTGCGCCTGAATCAGCGCGCGGGCGGCGCACAACAGCCGCGTTTCATGCGCCTGGACGTCAAGAGC
CGACCGCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTCTTGGT
GTTCCTCAACCGTCGCGGCTTTGCGCCAACCTTGCTGTGCCACGACTGCGGCTGGATGTCCGGCTGCCAACGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAACTGCGCTGCCATCACTGCGGGTATGTCGAGCGCGTACCGCGGCAGTGC
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAACGCCTGGCGATTCTGTTTCC
GGACTACCCCGTGCTGCGTGTCGATCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGTCAGCCGTGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACCCTGGTCTCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAACTGATCGTGCAGGTGGCAGG
CCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCATCCGTTGCTGATCCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAAGCCTTGAGCGAGCGCCGTTCAGCGAGTCTGCCACCGTTTTCACACCTT
GCACTGCTGCGCGCCGAAGCGCACAAGCCGGGACAGGCCGAGACCTTTCTCGATCAGGCCTGCAGCGATGCCGAGCAACT
GGTTGCGCAAATGGCGCTGGGCGGCATAGAGCTGCTGGGCCCGGTTCCGGCCCCGATGGAACGCCGCGCCGGCCGTTATC
GCGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCGCCACTGCATAAATTGCTTGCCACCTGGCTGCTCGCACTGGAGCAA
ATGCCCAGCGGTCGACAGGTCAGATGGTCGCTGGATGTGGATCCCGTGGATCTTTATTAA

Upstream 100 bases:

>100_bases
GATAGTACCAGACAATTGCAACGACGCAAGCGAACCTTCACACCGGTCGTCTGCTAAGATCGCGCCTCCGTCATCACTTA
TCACCACAGGGAAACACCGC

Downstream 100 bases:

>100_bases
GTCACGCCGTTGGCAAGCAGGCTTGAGCAACGGATAATGCAGAGTTTTTCCACCCGCGCATCGAAGCGCCACCGCGCTTG
CGGTCGAAGAGAGCACCATG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNRDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALIHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSASLPPFSHL
ALLRAEAHKPGQAETFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Sequences:

>Translated_739_residues
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNRDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALIHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSASLPPFSHL
ALLRAEAHKPGQAETFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY
>Mature_738_residues
PDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPAL
FKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLLS
KLMLNRDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQL
IRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALIHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGLI
IIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKSR
PLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQCP
SCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSIL
DADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSASLPPFSHLA
LLRAEAHKPGQAETFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQM
PSGRQVRWSLDVDPVDLY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=738, Percent_Identity=50.5420054200542, Blast_Score=698, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=213, Percent_Identity=29.5774647887324, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81973; Mature: 81842

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVP
CCCHHEEECCCHHHHHHHHCCCCCCCCHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCC
ADKLKPAIALLDSEAPLPPALFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQER
HHHHCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHC
FWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLLSKLMLNRDSLNLLLAKELVY
EEEECCCCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHEEHHEEE
VEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
EEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALIHSAVNDRERLDAWLAARDGE
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEEHHHCCHHHHHHHHHCCCCC
ADIIIGTRSALFTPMKNPGLIIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGS
CEEEEECHHHHCCCCCCCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCEEEEC
ATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKSRPLDSGISGPMQQAIGQTLA
CCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
AGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
CCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHCHHHEEECCCCCEEEECCCHHHHHHHHC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCIL
CCCCCCEEEECCCCCHHHHCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEE
VGTQMLAKGHHFPRVTLVSILDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVII
ECHHHHHCCCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
QTHLADHPLLIQLTEQGYFAFAEQALSERRSASLPPFSHLALLRAEAHKPGQAETFLDQA
EEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
CSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
HCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MPSGRQVRWSLDVDPVDLY
CCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
PDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVP
CCHHEEECCCHHHHHHHHCCCCCCCCHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCC
ADKLKPAIALLDSEAPLPPALFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQER
HHHHCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHC
FWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLLSKLMLNRDSLNLLLAKELVY
EEEECCCCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHEEHHEEE
VEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
EEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALIHSAVNDRERLDAWLAARDGE
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEEHHHCCHHHHHHHHHCCCCC
ADIIIGTRSALFTPMKNPGLIIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGS
CEEEEECHHHHCCCCCCCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCEEEEC
ATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKSRPLDSGISGPMQQAIGQTLA
CCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
AGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
CCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHCHHHEEECCCCCEEEECCCHHHHHHHHC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCIL
CCCCCCEEEECCCCCHHHHCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEE
VGTQMLAKGHHFPRVTLVSILDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVII
ECHHHHHCCCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
QTHLADHPLLIQLTEQGYFAFAEQALSERRSASLPPFSHLALLRAEAHKPGQAETFLDQA
EEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
CSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
HCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MPSGRQVRWSLDVDPVDLY
CCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]