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The map label for this gene is gcvH [H]

Identifier: 66043517

GI number: 66043517

Start: 260791

End: 261177

Strand: Direct

Name: gcvH [H]

Synonym: Psyr_0247

Alternate gene names: 66043517

Gene position: 260791-261177 (Clockwise)

Preceding gene: 66043516

Following gene: 66043521

Centisome position: 4.28

GC content: 59.95

Gene sequence:

>387_bases
ATGAGCAATATTCCCGCCGAGCTGCGTTTCGCCGAAAGTCATGAATGGGCACGTCTGGAGTCAGACGGTACCGTGACCGT
CGGCATCTCGGACCATGCACAGGAAGCACTGGGCGATGTGGTCTTCGTCGAACTGCCGGAAATCGGCACAGCGTTCGCTG
CCGGTGACACCGCCGGTGTTGTCGAGTCGGTCAAGGCCGCTTCGGATATCTACTCGCCGGTTGCGGGCGAGGTGATCGAA
GTCAACACAGCGTTGGGCGATGCCCCCGAGTCGCTCAACAGTGATTCGTACGGTGCCTGGATCTTCAAGCTCAAGCCAAG
CGACGCCAGCGGTGATCTGGCCAAGCTGCTGGATGCAGCCGGGTACAAGAGCGCTATCGGCGAATAA

Upstream 100 bases:

>100_bases
TACCCGGTGCGGGTGGTGCAGCCTGCGTTCGTGCGCCATGGCAAAACCTTGATCTAACCTATGGCGGGTTTTCCGCTGAC
CCGATGTTGAGGATGACTAA

Downstream 100 bases:

>100_bases
CCGATTACCGCTCACAAAAATGCCGCTCATGGAGCGGCATTTTTTATGCGCGAAGCTAACCGGCAAGACTTACTGGTTTT
CAGCGACCAGATTCTTATCC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MSNIPAELRFAESHEWARLESDGTVTVGISDHAQEALGDVVFVELPEIGTAFAAGDTAGVVESVKAASDIYSPVAGEVIE
VNTALGDAPESLNSDSYGAWIFKLKPSDASGDLAKLLDAAGYKSAIGE

Sequences:

>Translated_128_residues
MSNIPAELRFAESHEWARLESDGTVTVGISDHAQEALGDVVFVELPEIGTAFAAGDTAGVVESVKAASDIYSPVAGEVIE
VNTALGDAPESLNSDSYGAWIFKLKPSDASGDLAKLLDAAGYKSAIGE
>Mature_127_residues
SNIPAELRFAESHEWARLESDGTVTVGISDHAQEALGDVVFVELPEIGTAFAAGDTAGVVESVKAASDIYSPVAGEVIEV
NTALGDAPESLNSDSYGAWIFKLKPSDASGDLAKLLDAAGYKSAIGE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=47.0588235294118, Blast_Score=105, Evalue=9e-24,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=47.0588235294118, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=59.8425196850394, Blast_Score=157, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17507493, Length=114, Percent_Identity=46.4912280701754, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=114, Percent_Identity=44.7368421052632, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6319272, Length=102, Percent_Identity=44.1176470588235, Blast_Score=97, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17865652, Length=124, Percent_Identity=45.1612903225806, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13242; Mature: 13110

Theoretical pI: Translated: 3.88; Mature: 3.88

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPAELRFAESHEWARLESDGTVTVGISDHAQEALGDVVFVELPEIGTAFAAGDTAGV
CCCCCCCEEECCCCCCEEECCCCEEEEEECHHHHHHHCCEEEEECCCCCCEECCCCHHHH
VESVKAASDIYSPVAGEVIEVNTALGDAPESLNSDSYGAWIFKLKPSDASGDLAKLLDAA
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHC
GYKSAIGE
CCHHHCCC
>Mature Secondary Structure 
SNIPAELRFAESHEWARLESDGTVTVGISDHAQEALGDVVFVELPEIGTAFAAGDTAGV
CCCCCCEEECCCCCCEEECCCCEEEEEECHHHHHHHCCEEEEECCCCCCEECCCCHHHH
VESVKAASDIYSPVAGEVIEVNTALGDAPESLNSDSYGAWIFKLKPSDASGDLAKLLDAA
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHC
GYKSAIGE
CCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]