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The map label for this gene is gcvT [H]

Identifier: 66043516

GI number: 66043516

Start: 259665

End: 260747

Strand: Direct

Name: gcvT [H]

Synonym: Psyr_0246

Alternate gene names: 66043516

Gene position: 259665-260747 (Clockwise)

Preceding gene: 66043515

Following gene: 66043517

Centisome position: 4.26

GC content: 60.39

Gene sequence:

>1083_bases
ATGGGACAGCGTACCCCCCTGTTCGACCTGCACCTCGCCCTTGGCGCGAAAATGGTCGATTTTGGTGGCTGGGACATGCC
GTTGCATTACGGATCGCAGGTCGAGGAACACCATCAGGTGCGTCGCGACTGTGGGGTCTTCGATGTCTCTCACATGAACG
TGATCGATGTGCTCGGCCGTCAGGCCCGAGCCTGGCTGCGTCGTCTGCTGGCCAATGATGTAGACAAGCTCAAAACCCCC
GGTCGTGCCTTGTACAGCGCCATGCTGGACGAGAGCGGCGGGGTGATCGATGACATGATCGTCTACCTCACGCCTGAAGG
TTATCGGCTGGTCGTCAACGCGGCGACCGGCGTCAAGGATCTTGCCTGGATGCAGGCTCAACTGGGTGACTTCGACGTGC
AACTGGTCGAGCGCAGTGAAATGGCCATGCTCGCCATTCAAGGCCCCAAGGCCAGAGCCCGTATTGCCGAGCTGGTTTCA
AGCACCCGCGCCGAACTGATTCACCAGCTCAAGCCGTTCGAAGGGCTCGATGATGGCGACTGGTTTATCGCGCGCACCGG
CTACACCGGCGAGGACGGCCTGGAGATCATGCTGCCTGCCGAAGAGGCCCAAAGCTTTTTCAATGAATTGGTTGGCGCGG
GTATTTCCCCGATCGGCCTGGGCGCTCGCGACACGCTGCGCCTTGAGGCCGGCATGAACCTGTATGGCCAGGACATCGGC
GAGCATGTCTCGCCCTTGGCCTCGAATATGGCCTGGAGCATCGCCTGGGAGCCTGCCGACCGTGATTTTATCGGTCGCAA
GGCGCTGGAGAGCGAACGGGCTGAAGGAACTGCCTTCAAGCTCGTCGGATTGGTGCTCGAAGAGCGCGGTGTCTTGCGGG
CGCATCAGGTGGTACGTGTTGCAGAAATTGGCGAAGGAGAGATCACCAGTGGTAGTTTCTCTCCTACGTTGAGCAAATCC
ATTGCACTGGCTCGCGTTCCGATGGCGACCGCTGACCGGGCCGAGGTGGAAATCCGCGGCAAATGGTACCCGGTGCGGGT
GGTGCAGCCTGCGTTCGTGCGCCATGGCAAAACCTTGATCTAA

Upstream 100 bases:

>100_bases
AGATAATTCGGTCAGCTGTTACGTGCTGCAAACTCCGGTTCATGGGGGGCTTGCCGATCTCGTCTGCCCGCACCTTCGCC
ACGCCCGGAAGGAGAAACCC

Downstream 100 bases:

>100_bases
CCTATGGCGGGTTTTCCGCTGACCCGATGTTGAGGATGACTAAATGAGCAATATTCCCGCCGAGCTGCGTTTCGCCGAAA
GTCATGAATGGGCACGTCTG

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MGQRTPLFDLHLALGAKMVDFGGWDMPLHYGSQVEEHHQVRRDCGVFDVSHMNVIDVLGRQARAWLRRLLANDVDKLKTP
GRALYSAMLDESGGVIDDMIVYLTPEGYRLVVNAATGVKDLAWMQAQLGDFDVQLVERSEMAMLAIQGPKARARIAELVS
STRAELIHQLKPFEGLDDGDWFIARTGYTGEDGLEIMLPAEEAQSFFNELVGAGISPIGLGARDTLRLEAGMNLYGQDIG
EHVSPLASNMAWSIAWEPADRDFIGRKALESERAEGTAFKLVGLVLEERGVLRAHQVVRVAEIGEGEITSGSFSPTLSKS
IALARVPMATADRAEVEIRGKWYPVRVVQPAFVRHGKTLI

Sequences:

>Translated_360_residues
MGQRTPLFDLHLALGAKMVDFGGWDMPLHYGSQVEEHHQVRRDCGVFDVSHMNVIDVLGRQARAWLRRLLANDVDKLKTP
GRALYSAMLDESGGVIDDMIVYLTPEGYRLVVNAATGVKDLAWMQAQLGDFDVQLVERSEMAMLAIQGPKARARIAELVS
STRAELIHQLKPFEGLDDGDWFIARTGYTGEDGLEIMLPAEEAQSFFNELVGAGISPIGLGARDTLRLEAGMNLYGQDIG
EHVSPLASNMAWSIAWEPADRDFIGRKALESERAEGTAFKLVGLVLEERGVLRAHQVVRVAEIGEGEITSGSFSPTLSKS
IALARVPMATADRAEVEIRGKWYPVRVVQPAFVRHGKTLI
>Mature_359_residues
GQRTPLFDLHLALGAKMVDFGGWDMPLHYGSQVEEHHQVRRDCGVFDVSHMNVIDVLGRQARAWLRRLLANDVDKLKTPG
RALYSAMLDESGGVIDDMIVYLTPEGYRLVVNAATGVKDLAWMQAQLGDFDVQLVERSEMAMLAIQGPKARARIAELVSS
TRAELIHQLKPFEGLDDGDWFIARTGYTGEDGLEIMLPAEEAQSFFNELVGAGISPIGLGARDTLRLEAGMNLYGQDIGE
HVSPLASNMAWSIAWEPADRDFIGRKALESERAEGTAFKLVGLVLEERGVLRAHQVVRVAEIGEGEITSGSFSPTLSKSI
ALARVPMATADRAEVEIRGKWYPVRVVQPAFVRHGKTLI

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=369, Percent_Identity=35.5013550135501, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI257796258, Length=340, Percent_Identity=36.4705882352941, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI257796254, Length=364, Percent_Identity=31.043956043956, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI257796256, Length=314, Percent_Identity=33.1210191082803, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI24797151, Length=333, Percent_Identity=27.9279279279279, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI197927446, Length=334, Percent_Identity=26.3473053892216, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI21361378, Length=334, Percent_Identity=26.3473053892216, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI194306651, Length=319, Percent_Identity=25.705329153605, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789272, Length=364, Percent_Identity=56.8681318681319, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17560118, Length=375, Percent_Identity=31.4666666666667, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994045, Length=327, Percent_Identity=26.605504587156, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994052, Length=327, Percent_Identity=26.605504587156, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320222, Length=383, Percent_Identity=33.6814621409922, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI20129441, Length=374, Percent_Identity=32.0855614973262, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28571104, Length=280, Percent_Identity=26.4285714285714, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20130091, Length=368, Percent_Identity=23.9130434782609, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 39634; Mature: 39503

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00165 DEHYDRATASE_SER_THR ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQRTPLFDLHLALGAKMVDFGGWDMPLHYGSQVEEHHQVRRDCGVFDVSHMNVIDVLGR
CCCCCCCEEEHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCHHHHHHHH
QARAWLRRLLANDVDKLKTPGRALYSAMLDESGGVIDDMIVYLTPEGYRLVVNAATGVKD
HHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCHHH
LAWMQAQLGDFDVQLVERSEMAMLAIQGPKARARIAELVSSTRAELIHQLKPFEGLDDGD
HHHHHHHCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WFIARTGYTGEDGLEIMLPAEEAQSFFNELVGAGISPIGLGARDTLRLEAGMNLYGQDIG
EEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHH
EHVSPLASNMAWSIAWEPADRDFIGRKALESERAEGTAFKLVGLVLEERGVLRAHQVVRV
HHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHH
AEIGEGEITSGSFSPTLSKSIALARVPMATADRAEVEIRGKWYPVRVVQPAFVRHGKTLI
EECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEECCHHHHCCCCCCC
>Mature Secondary Structure 
GQRTPLFDLHLALGAKMVDFGGWDMPLHYGSQVEEHHQVRRDCGVFDVSHMNVIDVLGR
CCCCCCEEEHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCHHHHHHHH
QARAWLRRLLANDVDKLKTPGRALYSAMLDESGGVIDDMIVYLTPEGYRLVVNAATGVKD
HHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCHHH
LAWMQAQLGDFDVQLVERSEMAMLAIQGPKARARIAELVSSTRAELIHQLKPFEGLDDGD
HHHHHHHCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WFIARTGYTGEDGLEIMLPAEEAQSFFNELVGAGISPIGLGARDTLRLEAGMNLYGQDIG
EEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHH
EHVSPLASNMAWSIAWEPADRDFIGRKALESERAEGTAFKLVGLVLEERGVLRAHQVVRV
HHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHH
AEIGEGEITSGSFSPTLSKSIALARVPMATADRAEVEIRGKWYPVRVVQPAFVRHGKTLI
EECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEECCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]