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The map label for this gene is rep [H]

Identifier: 66043346

GI number: 66043346

Start: 79502

End: 81511

Strand: Direct

Name: rep [H]

Synonym: Psyr_0075

Alternate gene names: 66043346

Gene position: 79502-81511 (Clockwise)

Preceding gene: 66043344

Following gene: 66043347

Centisome position: 1.3

GC content: 60.55

Gene sequence:

>2010_bases
ATGTCCCGACTCAATCCCCGGCAGCAGGAAGCCGTGAACTACGTCGGCGGCCCTCTTTTGGTGCTCGCCGGTGCCGGTTC
CGGCAAGACCAGCGTGATCACCCGCAAGATCGCGCACCTGATCCAGAACTGCGGTATTCGCGCCCAGTACATCGTCGCCA
TGACCTTCACCAACAAGGCCGCGCGCGAGATGAAAGAGCGGGTCGGCACGCTGCTCAGAGGCGGCGAAGGACGTGGTCTG
ACGGTCTCGACCTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAGCACACACGGCTGGGCTACAAACCGGGCTTCTC
GATCTTTGACGAGACTGACGTCAAAGCGCTGATGACCGACATCATGCAGAAAGAGTATTCCGGCGACGACGGTGTCGACG
AAATCAAGAACATGATCGGCTCATGGAAAAACGACCTGATCCTGCCCGCCGAGGCGCTGGAAAACGCCCGCAACCCCAAG
GAACAGACCGCCGCCATCGTCTACACCCACTATCAGCGCACGCTCAAGGCGTACAACGCGGTGGACTTCGACGACCTGAT
TCTGTTGCCGGTCAAGCTGTTCCAGGAACACGCCGACATCCTGGAAAAGTGGCAGAACAAGGTGCGTTACCTGCTGGTCG
ACGAATATCAGGACACCAACGCCAGCCAGTACCTGCTGGTGAAGCTGCTGATCGGTACGCGCCATCAGTTCACCGTGGTG
GGCGACGATGACCAGTCGATCTACGCCTGGCGTGGTGCGCGGCCGGAAAACCTGATGCTGCTCAAGGACGACTACCCGTC
GTTGAAAGTGGTGATGCTCGAGCAGAACTACCGCTCGACCAGCCGTATTCTGCGCTGCGCCAACGTGCTGATCTCCAACA
ACCCGCACGCCTTCGAAAAACAACTGTGGTCCGAGATGGGCCATGGCGACGAGATCCGCGTGATCCGCTGCCGCAACGAA
GACGCCGAGGCCGAGCGGGTGGCCATGGAAATCCTCACCCTGCACCTGCGCACCGACCGCCCGTACAGCGATTTCGCCAT
CCTCTATCGCGGCAACTACCAGGCCAAGCTGATCGAGCTGAAATTGCAGCACCATCAGGTGCCGTATCGACTGTCGGGCG
GCAACAGCTTTTTCGGCCGCCAGGAAGTGAAGGACCTGATGGCTTATTTCCGTCTGCTGGTGAACCCGGACGACGACAAC
GCCTTCCTGCGGGTGATCAACGTTCCGCGTCGCGAGATCGGCTCGACCACGCTGGAAAAGCTCGGCAACTACGCCACCGA
GCGCAAGGTGTCGATGTACGCCGCCACCGACGAGATCGGTCTGGGCGAGCATCTGGACAGCCGATTCACCGACCGTTTAC
AGCGCTTCAAGCGCTGGATGGACGGCGTGCGCCAGCAGTGCGCGCAGAACGATCCCATCGCCGCGCTGCGCAGCATGGTC
ATGGACATCGACTACGAGAACTGGCTGCGGCAGAACAGCTCCAGCGAAAAGGCCGCCGATTACCGGATGAGCAATGTCTG
GTTCCTGATCGAGGCGTTGAAAAACACGCTGGAGAAGGACGAAGAAGGCGGCATGACCATCGAGGAAGCCATCGGCAAGC
TGGTCCTGCGCGACATGCTCGAACGTCAGCAGGAAGAGGAAGACGGTGCCGAAGGCGTGCAGATGATGACCTTGCACGCC
TCCAAGGGCCTGGAATTCCCTTATGTGTTCATCATGGGCATGGAAGAAGAAATCCTGCCACACCGCTCCAGCATCGAAGC
CGACACCATCGAAGAAGAGCGCCGCCTGGCCTACGTGGGCATCACCCGCGCGCGGCAGACCCTGGCCTTTACCTTCGCCG
CCAAGCGCAAGCAGTACGGTGAAATCATCGACTGCGCGCCGAGCCGCTTTCTGGATGAGCTGCCCCCGGACGACCTGGCC
TGGGAAGGCAATGACGACACCCCGACGGAAGTCAAAGCGGTTCGCGGCAACACTGCGCTTGCCGACATTCGCGCCATGCT
GAAACGCTAG

Upstream 100 bases:

>100_bases
AATTAGATAGCTGTCACTGTCTGACGACGATGAGCACTGCACAAAACCGTGGGCCTGTTAAAATGCCCGACCTTTTTTAC
AGACGACTCTTCATACCGCC

Downstream 100 bases:

>100_bases
CCGACACCCTATTGATCATTTATTGCGCAATGGCGCAGCCAAGGAATTTAAGTGGAAGCACTGCACAAGAAAATTCGCGA
AGAAGGCATCGTATTGTCCG

Product: ATP-dependent DNA helicase RepA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLIQNCGIRAQYIVAMTFTNKAAREMKERVGTLLRGGEGRGL
TVSTFHNLGLNIIRKEHTRLGYKPGFSIFDETDVKALMTDIMQKEYSGDDGVDEIKNMIGSWKNDLILPAEALENARNPK
EQTAAIVYTHYQRTLKAYNAVDFDDLILLPVKLFQEHADILEKWQNKVRYLLVDEYQDTNASQYLLVKLLIGTRHQFTVV
GDDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQNYRSTSRILRCANVLISNNPHAFEKQLWSEMGHGDEIRVIRCRNE
DAEAERVAMEILTLHLRTDRPYSDFAILYRGNYQAKLIELKLQHHQVPYRLSGGNSFFGRQEVKDLMAYFRLLVNPDDDN
AFLRVINVPRREIGSTTLEKLGNYATERKVSMYAATDEIGLGEHLDSRFTDRLQRFKRWMDGVRQQCAQNDPIAALRSMV
MDIDYENWLRQNSSSEKAADYRMSNVWFLIEALKNTLEKDEEGGMTIEEAIGKLVLRDMLERQQEEEDGAEGVQMMTLHA
SKGLEFPYVFIMGMEEEILPHRSSIEADTIEEERRLAYVGITRARQTLAFTFAAKRKQYGEIIDCAPSRFLDELPPDDLA
WEGNDDTPTEVKAVRGNTALADIRAMLKR

Sequences:

>Translated_669_residues
MSRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLIQNCGIRAQYIVAMTFTNKAAREMKERVGTLLRGGEGRGL
TVSTFHNLGLNIIRKEHTRLGYKPGFSIFDETDVKALMTDIMQKEYSGDDGVDEIKNMIGSWKNDLILPAEALENARNPK
EQTAAIVYTHYQRTLKAYNAVDFDDLILLPVKLFQEHADILEKWQNKVRYLLVDEYQDTNASQYLLVKLLIGTRHQFTVV
GDDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQNYRSTSRILRCANVLISNNPHAFEKQLWSEMGHGDEIRVIRCRNE
DAEAERVAMEILTLHLRTDRPYSDFAILYRGNYQAKLIELKLQHHQVPYRLSGGNSFFGRQEVKDLMAYFRLLVNPDDDN
AFLRVINVPRREIGSTTLEKLGNYATERKVSMYAATDEIGLGEHLDSRFTDRLQRFKRWMDGVRQQCAQNDPIAALRSMV
MDIDYENWLRQNSSSEKAADYRMSNVWFLIEALKNTLEKDEEGGMTIEEAIGKLVLRDMLERQQEEEDGAEGVQMMTLHA
SKGLEFPYVFIMGMEEEILPHRSSIEADTIEEERRLAYVGITRARQTLAFTFAAKRKQYGEIIDCAPSRFLDELPPDDLA
WEGNDDTPTEVKAVRGNTALADIRAMLKR
>Mature_668_residues
SRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLIQNCGIRAQYIVAMTFTNKAAREMKERVGTLLRGGEGRGLT
VSTFHNLGLNIIRKEHTRLGYKPGFSIFDETDVKALMTDIMQKEYSGDDGVDEIKNMIGSWKNDLILPAEALENARNPKE
QTAAIVYTHYQRTLKAYNAVDFDDLILLPVKLFQEHADILEKWQNKVRYLLVDEYQDTNASQYLLVKLLIGTRHQFTVVG
DDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQNYRSTSRILRCANVLISNNPHAFEKQLWSEMGHGDEIRVIRCRNED
AEAERVAMEILTLHLRTDRPYSDFAILYRGNYQAKLIELKLQHHQVPYRLSGGNSFFGRQEVKDLMAYFRLLVNPDDDNA
FLRVINVPRREIGSTTLEKLGNYATERKVSMYAATDEIGLGEHLDSRFTDRLQRFKRWMDGVRQQCAQNDPIAALRSMVM
DIDYENWLRQNSSSEKAADYRMSNVWFLIEALKNTLEKDEEGGMTIEEAIGKLVLRDMLERQQEEEDGAEGVQMMTLHAS
KGLEFPYVFIMGMEEEILPHRSSIEADTIEEERRLAYVGITRARQTLAFTFAAKRKQYGEIIDCAPSRFLDELPPDDLAW
EGNDDTPTEVKAVRGNTALADIRAMLKR

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=669, Percent_Identity=54.1106128550075, Blast_Score=721, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=645, Percent_Identity=37.984496124031, Blast_Score=388, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6322369, Length=459, Percent_Identity=31.1546840958606, Blast_Score=190, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6324477, Length=663, Percent_Identity=22.1719457013575, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76599; Mature: 76468

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLIQNCGIRAQYIVAMTFTNKA
CCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCHH
AREMKERVGTLLRGGEGRGLTVSTFHNLGLNIIRKEHTRLGYKPGFSIFDETDVKALMTD
HHHHHHHHHHHHCCCCCCCEEEEHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
IMQKEYSGDDGVDEIKNMIGSWKNDLILPAEALENARNPKEQTAAIVYTHYQRTLKAYNA
HHHHHCCCCCCHHHHHHHHHCCCCCEEECHHHHHCCCCCCHHHHEEEHHHHHHHHHHHCC
VDFDDLILLPVKLFQEHADILEKWQNKVRYLLVDEYQDTNASQYLLVKLLIGTRHQFTVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQNYRSTSRILRCANVLISNNPHAFEK
ECCCCCEEEECCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHH
QLWSEMGHGDEIRVIRCRNEDAEAERVAMEILTLHLRTDRPYSDFAILYRGNYQAKLIEL
HHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCEEEEEE
KLQHHQVPYRLSGGNSFFGRQEVKDLMAYFRLLVNPDDDNAFLRVINVPRREIGSTTLEK
EEHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHCHHHHHH
LGNYATERKVSMYAATDEIGLGEHLDSRFTDRLQRFKRWMDGVRQQCAQNDPIAALRSMV
HHCHHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MDIDYENWLRQNSSSEKAADYRMSNVWFLIEALKNTLEKDEEGGMTIEEAIGKLVLRDML
HHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
ERQQEEEDGAEGVQMMTLHASKGLEFPYVFIMGMEEEILPHRSSIEADTIEEERRLAYVG
HHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCCHHHHHHHHHEEEEE
ITRARQTLAFTFAAKRKQYGEIIDCAPSRFLDELPPDDLAWEGNDDTPTEVKAVRGNTAL
HHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHH
ADIRAMLKR
HHHHHHHCC
>Mature Secondary Structure 
SRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLIQNCGIRAQYIVAMTFTNKA
CCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCHH
AREMKERVGTLLRGGEGRGLTVSTFHNLGLNIIRKEHTRLGYKPGFSIFDETDVKALMTD
HHHHHHHHHHHHCCCCCCCEEEEHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
IMQKEYSGDDGVDEIKNMIGSWKNDLILPAEALENARNPKEQTAAIVYTHYQRTLKAYNA
HHHHHCCCCCCHHHHHHHHHCCCCCEEECHHHHHCCCCCCHHHHEEEHHHHHHHHHHHCC
VDFDDLILLPVKLFQEHADILEKWQNKVRYLLVDEYQDTNASQYLLVKLLIGTRHQFTVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQNYRSTSRILRCANVLISNNPHAFEK
ECCCCCEEEECCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHH
QLWSEMGHGDEIRVIRCRNEDAEAERVAMEILTLHLRTDRPYSDFAILYRGNYQAKLIEL
HHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCEEEEEE
KLQHHQVPYRLSGGNSFFGRQEVKDLMAYFRLLVNPDDDNAFLRVINVPRREIGSTTLEK
EEHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHCHHHHHH
LGNYATERKVSMYAATDEIGLGEHLDSRFTDRLQRFKRWMDGVRQQCAQNDPIAALRSMV
HHCHHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MDIDYENWLRQNSSSEKAADYRMSNVWFLIEALKNTLEKDEEGGMTIEEAIGKLVLRDML
HHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
ERQQEEEDGAEGVQMMTLHASKGLEFPYVFIMGMEEEILPHRSSIEADTIEEERRLAYVG
HHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCCHHHHHHHHHEEEEE
ITRARQTLAFTFAAKRKQYGEIIDCAPSRFLDELPPDDLAWEGNDDTPTEVKAVRGNTAL
HHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHH
ADIRAMLKR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]