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The map label for this gene is norM [H]

Identifier: 66043344

GI number: 66043344

Start: 76238

End: 77620

Strand: Direct

Name: norM [H]

Synonym: Psyr_0073

Alternate gene names: 66043344

Gene position: 76238-77620 (Clockwise)

Preceding gene: 66043341

Following gene: 66043346

Centisome position: 1.25

GC content: 64.06

Gene sequence:

>1383_bases
ATGCAGCATTCCGCCCTCAAAGAACTCTGGATCATCCTGCGCCTGGCGGGGCCGCTGATCGCGTCACAGATGGCGCACAT
GCTGATGGTCTTCACCGACACCGTGATGATGGGCAAGATCGGCCCGGAAGCCTTGGCCGGCGGTGGTCTGGGCGCTGCCA
CGTACAGCTTCGTGTCGTTCTTTTGCGTCGGCGTGATGGCCGCGGTCGGCACGCTGGTCTCGATTCGTCATGGTGCCGGC
GACACCGAAGGCGCGACTCGTCTGACCCAGGCCGGTCTGTGGCTGGCCTGGGGCATGGCGCTGGTTGCCGCGCTGTTGCT
GTGGAACCTCGAACCCATCCTGCTGCAGTTCGGTCAGGCCGAAGCCAACGTGCATATGGCGGCTCAATTTCTGATCACCC
TGCCCTTCGCCCTGCCCGGCCTGCTCAGCTTCATGGCATTGCGCGGCTTCACCAGCGCACTGGGTCGCGCGGGGCCGGTG
ATGACCATCAGCCTGGCCGGTGCGGCAGCCAACTTCGTGCTCAATTACGCGTTGATCCATCAATGGCTGGGGCTGCCCAA
CCTGGGGCTGATGGGCATCGGGCTGGTCACCGCGATCGTCACCAACTGCATGGCCCTGGCGCTGGCGCTGCATATCAGGC
TGCATCCGGCCTACGCCGCATACCCGATCAGAAAGGGGCTTTCGAAACTGTCACGCAGCCACCTGGGCGAGCTGTGGCGC
CTCGGTTTGCCGATTGGCGGCACCTATGCAGTGGAAGTCGGGCTGTTCACTTTTGCGGCGTTCTGCATGGGCGCGATGGG
CAGCACGCAGATGGCGGCGCACCAGATCGCCCTGCAGACTGTGTCCATGGCGTTCATGGTTCCGGTGGGGATGTCCTACG
CGGTCACCATGCGTATCGGCCAGCATTATGGCGCGGGCAACCTGTTGATGGTGCGCATGGCCGGGCGTCTGGGTATCGGT
TTCGGCGGCTGTGTGATGCTCATGTTCGGGCTGCTGTTCTGGCTGGCACCGCATCTGGTCATCGGCCTGTTTCTGGACAT
CAATGACCCGGCGTTCGCCGAGATCGTGGTACTGGCCACAAAACTGCTGGCGATTGCCGCCTTGTTCGAGTTTTTCGACG
GCACGCAGACCATTGCCATGGGCGCCATTCGCGGGCTCAAGGATGCACGCACCACCTTCCTGATCGGCCTGGGCTGCTAC
TGGCTGATCGCTGCGCCTGCCGCGTGGTTGCTCGGCTTCTCTACCGATGCTGGCGCTTCAGGCGTGTGGTGGGGGCTGGC
GCTGGGCCTGTTCTGCTCGGCCGTGGCGCTGACCTACGCATTTGAATGGAAGACGGCGCGGCTGTTGCGTCAGGACGCAG
CCGGGTTGCCGGTGCCGGGCTGA

Upstream 100 bases:

>100_bases
TTTAGTCATGCTGAGCATCGGATTTATCGTTTTGTGTTTAGCCGGGCAACTGCCCAGAATACGTCGCTCAATCGTCCCTT
TGCCGTAGAGCACCGTCACC

Downstream 100 bases:

>100_bases
TCCGGTGCACCGTCACCCGGATCTGGAGGTATCGGCGTGTGCCACTTCCGAACGCAGATAGGTCACCAGTTCTTCCACCG
GCAGCGGCCGGCTGATCAGA

Product: multidrug efflux protein NorA

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MQHSALKELWIILRLAGPLIASQMAHMLMVFTDTVMMGKIGPEALAGGGLGAATYSFVSFFCVGVMAAVGTLVSIRHGAG
DTEGATRLTQAGLWLAWGMALVAALLLWNLEPILLQFGQAEANVHMAAQFLITLPFALPGLLSFMALRGFTSALGRAGPV
MTISLAGAAANFVLNYALIHQWLGLPNLGLMGIGLVTAIVTNCMALALALHIRLHPAYAAYPIRKGLSKLSRSHLGELWR
LGLPIGGTYAVEVGLFTFAAFCMGAMGSTQMAAHQIALQTVSMAFMVPVGMSYAVTMRIGQHYGAGNLLMVRMAGRLGIG
FGGCVMLMFGLLFWLAPHLVIGLFLDINDPAFAEIVVLATKLLAIAALFEFFDGTQTIAMGAIRGLKDARTTFLIGLGCY
WLIAAPAAWLLGFSTDAGASGVWWGLALGLFCSAVALTYAFEWKTARLLRQDAAGLPVPG

Sequences:

>Translated_460_residues
MQHSALKELWIILRLAGPLIASQMAHMLMVFTDTVMMGKIGPEALAGGGLGAATYSFVSFFCVGVMAAVGTLVSIRHGAG
DTEGATRLTQAGLWLAWGMALVAALLLWNLEPILLQFGQAEANVHMAAQFLITLPFALPGLLSFMALRGFTSALGRAGPV
MTISLAGAAANFVLNYALIHQWLGLPNLGLMGIGLVTAIVTNCMALALALHIRLHPAYAAYPIRKGLSKLSRSHLGELWR
LGLPIGGTYAVEVGLFTFAAFCMGAMGSTQMAAHQIALQTVSMAFMVPVGMSYAVTMRIGQHYGAGNLLMVRMAGRLGIG
FGGCVMLMFGLLFWLAPHLVIGLFLDINDPAFAEIVVLATKLLAIAALFEFFDGTQTIAMGAIRGLKDARTTFLIGLGCY
WLIAAPAAWLLGFSTDAGASGVWWGLALGLFCSAVALTYAFEWKTARLLRQDAAGLPVPG
>Mature_460_residues
MQHSALKELWIILRLAGPLIASQMAHMLMVFTDTVMMGKIGPEALAGGGLGAATYSFVSFFCVGVMAAVGTLVSIRHGAG
DTEGATRLTQAGLWLAWGMALVAALLLWNLEPILLQFGQAEANVHMAAQFLITLPFALPGLLSFMALRGFTSALGRAGPV
MTISLAGAAANFVLNYALIHQWLGLPNLGLMGIGLVTAIVTNCMALALALHIRLHPAYAAYPIRKGLSKLSRSHLGELWR
LGLPIGGTYAVEVGLFTFAAFCMGAMGSTQMAAHQIALQTVSMAFMVPVGMSYAVTMRIGQHYGAGNLLMVRMAGRLGIG
FGGCVMLMFGLLFWLAPHLVIGLFLDINDPAFAEIVVLATKLLAIAALFEFFDGTQTIAMGAIRGLKDARTTFLIGLGCY
WLIAAPAAWLLGFSTDAGASGVWWGLALGLFCSAVALTYAFEWKTARLLRQDAAGLPVPG

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=447, Percent_Identity=23.2662192393736, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI38261960, Length=487, Percent_Identity=23.8193018480493, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI22907060, Length=459, Percent_Identity=22.6579520697168, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI48994890, Length=438, Percent_Identity=30.365296803653, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 48655; Mature: 48655

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHSALKELWIILRLAGPLIASQMAHMLMVFTDTVMMGKIGPEALAGGGLGAATYSFVSF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHH
FCVGVMAAVGTLVSIRHGAGDTEGATRLTQAGLWLAWGMALVAALLLWNLEPILLQFGQA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
EANVHMAAQFLITLPFALPGLLSFMALRGFTSALGRAGPVMTISLAGAAANFVLNYALIH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
QWLGLPNLGLMGIGLVTAIVTNCMALALALHIRLHPAYAAYPIRKGLSKLSRSHLGELWR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGLPIGGTYAVEVGLFTFAAFCMGAMGSTQMAAHQIALQTVSMAFMVPVGMSYAVTMRIG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEHH
QHYGAGNLLMVRMAGRLGIGFGGCVMLMFGLLFWLAPHLVIGLFLDINDPAFAEIVVLAT
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
KLLAIAALFEFFDGTQTIAMGAIRGLKDARTTFLIGLGCYWLIAAPAAWLLGFSTDAGAS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GVWWGLALGLFCSAVALTYAFEWKTARLLRQDAAGLPVPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQHSALKELWIILRLAGPLIASQMAHMLMVFTDTVMMGKIGPEALAGGGLGAATYSFVSF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHH
FCVGVMAAVGTLVSIRHGAGDTEGATRLTQAGLWLAWGMALVAALLLWNLEPILLQFGQA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
EANVHMAAQFLITLPFALPGLLSFMALRGFTSALGRAGPVMTISLAGAAANFVLNYALIH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
QWLGLPNLGLMGIGLVTAIVTNCMALALALHIRLHPAYAAYPIRKGLSKLSRSHLGELWR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGLPIGGTYAVEVGLFTFAAFCMGAMGSTQMAAHQIALQTVSMAFMVPVGMSYAVTMRIG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEHH
QHYGAGNLLMVRMAGRLGIGFGGCVMLMFGLLFWLAPHLVIGLFLDINDPAFAEIVVLAT
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
KLLAIAALFEFFDGTQTIAMGAIRGLKDARTTFLIGLGCYWLIAAPAAWLLGFSTDAGAS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GVWWGLALGLFCSAVALTYAFEWKTARLLRQDAAGLPVPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]