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The map label for this gene is ispG [H]

Identifier: 62390853

GI number: 62390853

Start: 2097397

End: 2098608

Strand: Reverse

Name: ispG [H]

Synonym: cg2206

Alternate gene names: 62390853

Gene position: 2098608-2097397 (Counterclockwise)

Preceding gene: 62390854

Following gene: 62390850

Centisome position: 63.93

GC content: 55.86

Gene sequence:

>1212_bases
GTGCGGCTTCCTTTTCTAGGAGCATGTAACTTGTCTACCCCAATCGGCCTTGGCCTTCCTCCGACCCCACCTCCAGTGTT
GGCGCCACGTCGTAAAACACGCCAACTCATGGTCGGCAAAGTGGGCGTTGGTTCGGATCACCCGATTTCCGTCCAGTCGA
TGACCACCACCAAAACCCACGACATCAACGGCACCCTGCAACAGATCGCACAGTTGACAGCCACCGGTTGTGACATCGTC
CGCGTTGCCTGCCCAAAGACTGTTGATGCGGAAGCACTGCCGATCATCGCAAAGAAGTCTCCGATCCCAGTGATCGCAGA
TATCCACTTCCAGCCCAAGTACATCTTCGCGGCAATCGATGCAGGTTGCGCCGCCGTTCGTGTGAACCCAGGCAACATCA
AGGAATTCGATGGTCGCGTTAAAGAAGTAGCAAAAGCTGCAGGCGATGCCGGAATTCCAATTCGTATTGGTGTCAACGGC
GGATCCCTGGATAAGCGCATCCTGGACAAATACCACGGCAAAGCCACCCCAGAAGCTCTCGTGGAATCCGCAATGTGGGA
AGCCGGCCTGTTTGAAGAGCACGGCTTCGGCGACATCGCAATCTCTGTGAAGCACTCCGACCCAGTACTCATGGTGGAGG
CCTACCGCCAGCTCGCTGAACAAAGCGACTACCCACTGCACCTCGGTGTTACTGAAGCTGGTCCCAAGTTCATGGGAACA
ATCAAGTCTTCCGTAGCATTCGGCGCTCTGCTGTCCCAGGGCATCGGCGACACTATCCGTGTGTCTCTTTCTGCTGACCC
AGTGGAAGAAATCAAGGTTGGCGACCAGATTCTGCAGTCCCTCAACCTGCGCCCACGCAAGCTGGAAATCGTGTCCTGCC
CATCATGTGGCCGCGCACAGGTCGATGTGTACTCACTTGCTGAAGAAGTCACCGAAGCACTCGACGGCATGGAAGTTCCA
CTGCGCGTCGCTGTCATGGGTTGCGTTGTTAACGGCCCAGGTGAGGCTCGCGACGCTGACCTCGGTGTTGCATCCGGTAA
CGGCAAGGGCCAGATCTTTGTCAAGGGCGAAGTCATCAAGACTGTCCCAGAATCCCAGATCGTGGAAACCCTCATCGAAG
AAGCAATGCGTATCGCAGAGGAAATGGACCCAGAAGTCCTCGCTGCAGCAAGTGCTTCCGGTATGAAGGCTGAAGTGAAG
GTAACCAAGTAA

Upstream 100 bases:

>100_bases
GAATTGAACTGCCGGCACCGATGTTAAAAGAGGTGACGGTGATACAATTTTGCCATAACTGACTTAACCCAGAACGTTGT
CCGGAAGCACACAGACCTTT

Downstream 100 bases:

>100_bases
GTTTTGGTTAATTAAGGCACCCGACTGGTTTGGTTAGTCGGGTGCTTTTTTGATGGGTAAATTCCGTAAACTAAACCAGA
TGACTGGTGGGGAACGTGAG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MRLPFLGACNLSTPIGLGLPPTPPPVLAPRRKTRQLMVGKVGVGSDHPISVQSMTTTKTHDINGTLQQIAQLTATGCDIV
RVACPKTVDAEALPIIAKKSPIPVIADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNG
GSLDKRILDKYHGKATPEALVESAMWEAGLFEEHGFGDIAISVKHSDPVLMVEAYRQLAEQSDYPLHLGVTEAGPKFMGT
IKSSVAFGALLSQGIGDTIRVSLSADPVEEIKVGDQILQSLNLRPRKLEIVSCPSCGRAQVDVYSLAEEVTEALDGMEVP
LRVAVMGCVVNGPGEARDADLGVASGNGKGQIFVKGEVIKTVPESQIVETLIEEAMRIAEEMDPEVLAAASASGMKAEVK
VTK

Sequences:

>Translated_403_residues
MRLPFLGACNLSTPIGLGLPPTPPPVLAPRRKTRQLMVGKVGVGSDHPISVQSMTTTKTHDINGTLQQIAQLTATGCDIV
RVACPKTVDAEALPIIAKKSPIPVIADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNG
GSLDKRILDKYHGKATPEALVESAMWEAGLFEEHGFGDIAISVKHSDPVLMVEAYRQLAEQSDYPLHLGVTEAGPKFMGT
IKSSVAFGALLSQGIGDTIRVSLSADPVEEIKVGDQILQSLNLRPRKLEIVSCPSCGRAQVDVYSLAEEVTEALDGMEVP
LRVAVMGCVVNGPGEARDADLGVASGNGKGQIFVKGEVIKTVPESQIVETLIEEAMRIAEEMDPEVLAAASASGMKAEVK
VTK
>Mature_403_residues
MRLPFLGACNLSTPIGLGLPPTPPPVLAPRRKTRQLMVGKVGVGSDHPISVQSMTTTKTHDINGTLQQIAQLTATGCDIV
RVACPKTVDAEALPIIAKKSPIPVIADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNG
GSLDKRILDKYHGKATPEALVESAMWEAGLFEEHGFGDIAISVKHSDPVLMVEAYRQLAEQSDYPLHLGVTEAGPKFMGT
IKSSVAFGALLSQGIGDTIRVSLSADPVEEIKVGDQILQSLNLRPRKLEIVSCPSCGRAQVDVYSLAEEVTEALDGMEVP
LRVAVMGCVVNGPGEARDADLGVASGNGKGQIFVKGEVIKTVPESQIVETLIEEAMRIAEEMDPEVLAAASASGMKAEVK
VTK

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=354, Percent_Identity=44.3502824858757, Blast_Score=300, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 42579; Mature: 42579

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPFLGACNLSTPIGLGLPPTPPPVLAPRRKTRQLMVGKVGVGSDHPISVQSMTTTKTH
CCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCEEEEEEEECCCC
DINGTLQQIAQLTATGCDIVRVACPKTVDAEALPIIAKKSPIPVIADIHFQPKYIFAAID
CCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCEEEEEEECCCEEEEEEC
AGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNGGSLDKRILDKYHGKATPEAL
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHH
VESAMWEAGLFEEHGFGDIAISVKHSDPVLMVEAYRQLAEQSDYPLHLGVTEAGPKFMGT
HHHHHHHCCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCCCCEEEEECCCCCHHHHH
IKSSVAFGALLSQGIGDTIRVSLSADPVEEIKVGDQILQSLNLRPRKLEIVSCPSCGRAQ
HHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
VDVYSLAEEVTEALDGMEVPLRVAVMGCVVNGPGEARDADLGVASGNGKGQIFVKGEVIK
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECCEEE
TVPESQIVETLIEEAMRIAEEMDPEVLAAASASGMKAEVKVTK
CCCHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCEEEEEEEC
>Mature Secondary Structure
MRLPFLGACNLSTPIGLGLPPTPPPVLAPRRKTRQLMVGKVGVGSDHPISVQSMTTTKTH
CCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCEEEEEEEECCCC
DINGTLQQIAQLTATGCDIVRVACPKTVDAEALPIIAKKSPIPVIADIHFQPKYIFAAID
CCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCEEEEEEECCCEEEEEEC
AGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNGGSLDKRILDKYHGKATPEAL
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHH
VESAMWEAGLFEEHGFGDIAISVKHSDPVLMVEAYRQLAEQSDYPLHLGVTEAGPKFMGT
HHHHHHHCCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCCCCEEEEECCCCCHHHHH
IKSSVAFGALLSQGIGDTIRVSLSADPVEEIKVGDQILQSLNLRPRKLEIVSCPSCGRAQ
HHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
VDVYSLAEEVTEALDGMEVPLRVAVMGCVVNGPGEARDADLGVASGNGKGQIFVKGEVIK
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECCEEE
TVPESQIVETLIEEAMRIAEEMDPEVLAAASASGMKAEVKVTK
CCCHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]