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The map label for this gene is metK [H]

Identifier: 62390485

GI number: 62390485

Start: 1700642

End: 1701865

Strand: Reverse

Name: metK [H]

Synonym: cg1806

Alternate gene names: 62390485

Gene position: 1701865-1700642 (Counterclockwise)

Preceding gene: 229068000

Following gene: 62390484

Centisome position: 51.84

GC content: 56.05

Gene sequence:

>1224_bases
GTGGCTCAGCCAACCGCCGTCCGTTTGTTCACCAGTGAATCTGTAACTGAGGGACATCCAGACAAAATATGTGATGCTAT
TTCCGATACCATTTTGGACGCGCTGCTCGAAAAAGATCCGCAGTCGCGCGTCGCAGTGGAAACTGTGGTCACCACCGGAA
TCGTCCATGTTGTTGGCGAGGTCCGTACCAGCGCTTACGTAGAGATCCCTCAATTAGTCCGCAACAAGCTCATCGAAATC
GGATTCAACTCCTCTGAGGTTGGATTCGACGGACGCACCTGTGGCGTCTCAGTATCCATCGGTGAGCAGTCCCAGGAAAT
CGCTGACGGCGTGGATAACTCCGACGAAGCCCGCACCAACGGCGACGTTGAAGAAGACGACCGCGCAGGTGCTGGCGACC
AGGGCCTGATGTTCGGCTACGCCACCAACGAAACCGAAGAGTACATGCCTCTTCCTATCGCGTTGGCGCACCGACTGTCA
CGTCGTCTGACCCAGGTTCGTAAAGAGGGCATCGTTCCTCACCTGCGTCCAGACGGAAAAACCCAGGTCACCTTCGCATA
CGATGCGCAAGACCGCCCTAGCCACCTGGATACCGTTGTCATCTCCACCCAGCACGACCCAGAAGTTGACCGTGCATGGT
TGGAAACCCAACTGCGCGAACACGTCATTGATTGGGTAATCAAAGACGCAGGCATTGAGGATCTGGCAACCGGTGAGATC
ACCGTGTTGATCAACCCTTCAGGTTCCTTCATTCTGGGTGGCCCCATGGGTGATGCGGGTCTGACCGGCCGCAAGATCAT
CGTGGATACCTACGGTGGCATGGCTCGCCATGGTGGTGGAGCATTCTCCGGTAAGGATCCAAGCAAGGTGGACCGCTCTG
CTGCATACGCCATGCGTTGGGTAGCAAAGAACATCGTGGCAGCAGGCCTTGCTGATCGCGCTGAAGTTCAGGTTGCATAC
GCCATTGGACGCGCAAAGCCAGTCGGACTTTACGTTGAAACCTTTGACACCAACAAGGAAGGCCTGAGCGACGAGCAGAT
TCAGGCTGCCGTGTTGGAGGTCTTTGACCTGCGTCCAGCAGCAATTATCCGTGAGCTTGATCTGCTTCGTCCGATCTACG
CTGACACTGCTGCCTACGGCCACTTTGGTCGCACTGATTTGGACCTTCCTTGGGAGGCTATCGACCGCGTTGATGAACTT
CGCGCAGCCCTCAAGTTGGCCTAA

Upstream 100 bases:

>100_bases
CAGCTAGACCACTGACATTGCAGTTTTAGACAGCTTGGTCTATATTGGTTTTTTGTATTTAAGACTATTTATTCTCAACT
TCTTCGAAAGAAGGGTATTT

Downstream 100 bases:

>100_bases
AAATCTGATGTAGTATCTTCGGATTCATGGCAAAAACCCGCGTCCCCGCTCCTGAAAAGTCGGTGGCGCGGGTTTTACCT
CTTTTGGGGTTACCTCACCT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MAQPTAVRLFTSESVTEGHPDKICDAISDTILDALLEKDPQSRVAVETVVTTGIVHVVGEVRTSAYVEIPQLVRNKLIEI
GFNSSEVGFDGRTCGVSVSIGEQSQEIADGVDNSDEARTNGDVEEDDRAGAGDQGLMFGYATNETEEYMPLPIALAHRLS
RRLTQVRKEGIVPHLRPDGKTQVTFAYDAQDRPSHLDTVVISTQHDPEVDRAWLETQLREHVIDWVIKDAGIEDLATGEI
TVLINPSGSFILGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLADRAEVQVAY
AIGRAKPVGLYVETFDTNKEGLSDEQIQAAVLEVFDLRPAAIIRELDLLRPIYADTAAYGHFGRTDLDLPWEAIDRVDEL
RAALKLA

Sequences:

>Translated_407_residues
MAQPTAVRLFTSESVTEGHPDKICDAISDTILDALLEKDPQSRVAVETVVTTGIVHVVGEVRTSAYVEIPQLVRNKLIEI
GFNSSEVGFDGRTCGVSVSIGEQSQEIADGVDNSDEARTNGDVEEDDRAGAGDQGLMFGYATNETEEYMPLPIALAHRLS
RRLTQVRKEGIVPHLRPDGKTQVTFAYDAQDRPSHLDTVVISTQHDPEVDRAWLETQLREHVIDWVIKDAGIEDLATGEI
TVLINPSGSFILGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLADRAEVQVAY
AIGRAKPVGLYVETFDTNKEGLSDEQIQAAVLEVFDLRPAAIIRELDLLRPIYADTAAYGHFGRTDLDLPWEAIDRVDEL
RAALKLA
>Mature_406_residues
AQPTAVRLFTSESVTEGHPDKICDAISDTILDALLEKDPQSRVAVETVVTTGIVHVVGEVRTSAYVEIPQLVRNKLIEIG
FNSSEVGFDGRTCGVSVSIGEQSQEIADGVDNSDEARTNGDVEEDDRAGAGDQGLMFGYATNETEEYMPLPIALAHRLSR
RLTQVRKEGIVPHLRPDGKTQVTFAYDAQDRPSHLDTVVISTQHDPEVDRAWLETQLREHVIDWVIKDAGIEDLATGEIT
VLINPSGSFILGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLADRAEVQVAYA
IGRAKPVGLYVETFDTNKEGLSDEQIQAAVLEVFDLRPAAIIRELDLLRPIYADTAAYGHFGRTDLDLPWEAIDRVDELR
AALKLA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=388, Percent_Identity=52.0618556701031, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI4557737, Length=390, Percent_Identity=50.7692307692308, Blast_Score=359, Evalue=3e-99,
Organism=Escherichia coli, GI1789311, Length=401, Percent_Identity=57.6059850374065, Blast_Score=440, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17538498, Length=392, Percent_Identity=53.8265306122449, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=53.4883720930233, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17538494, Length=387, Percent_Identity=53.2299741602067, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25145633, Length=403, Percent_Identity=51.3647642679901, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565905, Length=387, Percent_Identity=48.8372093023256, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI32565911, Length=342, Percent_Identity=52.3391812865497, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI25145635, Length=358, Percent_Identity=49.1620111731844, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI32565903, Length=315, Percent_Identity=52.0634920634921, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=51.8072289156626, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=51.6539440203562, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6323209, Length=387, Percent_Identity=52.4547803617571, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI45552159, Length=390, Percent_Identity=49.2307692307692, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24580529, Length=390, Percent_Identity=49.2307692307692, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24580537, Length=390, Percent_Identity=49.2307692307692, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24580533, Length=390, Percent_Identity=49.2307692307692, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24580535, Length=390, Percent_Identity=49.2307692307692, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24580531, Length=390, Percent_Identity=49.2307692307692, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24580527, Length=390, Percent_Identity=49.2307692307692, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24580525, Length=390, Percent_Identity=49.2307692307692, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=48, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24580539, Length=129, Percent_Identity=48.8372093023256, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44177; Mature: 44046

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPTAVRLFTSESVTEGHPDKICDAISDTILDALLEKDPQSRVAVETVVTTGIVHVVGE
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VRTSAYVEIPQLVRNKLIEIGFNSSEVGFDGRTCGVSVSIGEQSQEIADGVDNSDEARTN
HHHHHEEEHHHHHHHHHEEECCCCCCCCCCCCEEEEEEEECCCHHHHHCCCCCCCCCCCC
GDVEEDDRAGAGDQGLMFGYATNETEEYMPLPIALAHRLSRRLTQVRKEGIVPHLRPDGK
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TQVTFAYDAQDRPSHLDTVVISTQHDPEVDRAWLETQLREHVIDWVIKDAGIEDLATGEI
EEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
TVLINPSGSFILGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRW
EEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHH
VAKNIVAAGLADRAEVQVAYAIGRAKPVGLYVETFDTNKEGLSDEQIQAAVLEVFDLRPA
HHHHHHHHCCCCCHHEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHH
AIIRELDLLRPIYADTAAYGHFGRTDLDLPWEAIDRVDELRAALKLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQPTAVRLFTSESVTEGHPDKICDAISDTILDALLEKDPQSRVAVETVVTTGIVHVVGE
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VRTSAYVEIPQLVRNKLIEIGFNSSEVGFDGRTCGVSVSIGEQSQEIADGVDNSDEARTN
HHHHHEEEHHHHHHHHHEEECCCCCCCCCCCCEEEEEEEECCCHHHHHCCCCCCCCCCCC
GDVEEDDRAGAGDQGLMFGYATNETEEYMPLPIALAHRLSRRLTQVRKEGIVPHLRPDGK
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TQVTFAYDAQDRPSHLDTVVISTQHDPEVDRAWLETQLREHVIDWVIKDAGIEDLATGEI
EEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
TVLINPSGSFILGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRW
EEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHH
VAKNIVAAGLADRAEVQVAYAIGRAKPVGLYVETFDTNKEGLSDEQIQAAVLEVFDLRPA
HHHHHHHHCCCCCHHEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHH
AIIRELDLLRPIYADTAAYGHFGRTDLDLPWEAIDRVDELRAALKLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]