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The map label for this gene is priA [H]

Identifier: 62390484

GI number: 62390484

Start: 1698549

End: 1700615

Strand: Reverse

Name: priA [H]

Synonym: cg1805

Alternate gene names: 62390484

Gene position: 1700615-1698549 (Counterclockwise)

Preceding gene: 62390485

Following gene: 62390483

Centisome position: 51.81

GC content: 57.18

Gene sequence:

>2067_bases
ATGGCAAAAACCCGCGTCCCCGCTCCTGAAAAGTCGGTGGCGCGGGTTTTACCTCTTTTGGGGTTACCTCACCTGGATCG
ACTGTTTGATTACCGCATCAGCGAAGACCAACACGATGATGTGCAACCTGGCGTGCGGGTGCGCGTGCGTTTTGGTGGAC
GTTTAGTTGATGCCATCGTGATGTCACGCACCGCGCAAACCTCGCACGAGGGAAAGCTGATGTGGCTGGATCGGGTGATT
TCGCCGATCGTGGTGTATCCACCTCAAACAGCAAAGCTAATTGAGCAACTCAGTGATCGCTATGGCGGGGTACGTTCGGA
TCTCATCCGTTCGGCGCTACCGGCGCGGCATGCTGGGGCAGAAGAGGCAGATACCTCCACGTCGTGGGAGTCATTGGGTG
AGGTTAAAGAACCCGATTTATCGTCGTGGTCTGCGTATCAGCATGGTCAATCATTTGTTGACGCCGTCTTGGCGGGAACA
ACTGCGCGGGCGTCATGGCAGATTGCTCCCGGAGATGATTGGGCGCTGGCTTTGGCTTCTTTGGCGGTCAAGGTTGTCAA
AGACGGCGGCGGAGCGCTTCTCGTAGTGCCTGATCAGCGCGATCTCGACCGCTTGGAAGCTGCGCTTCGAGGTTTGGTTG
CGGCGAAACAAATCACTGTGCTTAATTCAGGTCTTGGTCCGCAGGCACGATATCGGCGTTTCCTATCGGTACTCAGTGGG
CAGGGACGACTGATTATTGGAACCAGAAGTGCCGCTTTTGCACCCGTGAAGGATCTGAAACTGGCCGTCATTTTAAATGA
TGGCGACGACAACCTCGTTGATCCTAGAGCGCCCTATGCCCACGCCAGGGAAGTGCTGACCACGCGTTCCAGTTTGGAAG
CAAGCTCCTTGATTATTGCGGGACATGCGCGGACCGCGGAAACCCAATTGCTGGTGGAATCGGGATGGATGCACAATCTC
ATCGCACCGAGGGATACCATTCGCACTAGGATGCCGCGTATTCAGGCAGTGGGCGATTCCGATTTCCAGATGGAACGCGA
TCCAATGGCCCGATCAGCGCGGCTGCCTGGCATTGCGTTTCATGCGGTGCGCAGCGCCTTAGAACGTGATCAACCAGCGC
TTATCCAGGTACCAAGGAAAGGCTACGTGCCAACCTTGGCGTGTGGAAACTGCCGCACCCCAGCGCGGTGCCGGCACTGT
AATGGGCCTGTGGGACTTCCCCAGGGAAGCTCTGATCTAGCGGGAGTGCCCACTTGCCGATGGTGCGGACGCCCTGATTC
GCGGTTTAAGTGCCAAAACTGCGGCTCTCCAAAACTGCGTGCTGTGGTGCTGGGAACGGAACGCACAGCAGAAGAACTGG
GCCGCGCGTTCCCGTCTGTGCGGGTAATTACCTCTGGTGGCAACAAGGTGGTGGATTCGGTGGAAAACCGAGCCAGCATT
GTGGTGTCCACGCCAGGTGCAGAACCTTTTGTGGCCAACTCGCCGGAGAGACCAGAGAAATCGGAGAAACCAGAGCACAA
GGGCGCTTACGGTGCCTTGTTATTGCTGGATACCTGGGCGTTGATGGGTCGGCAAGATCTGCGCGCCATGGAGGACGCGC
TGCACAAGTGGGCAGCGGCGGCCACGCTGGTGCATTCTCATCTGCACCAGGGTCAAGTCATCGTGGTTGCAGATCCATCG
TTTCCTGCTGTGCAATCGTTGATTCGGTGGGATATGGCAGGTGCTGCAGCGCAAGAGTTGGCTAGCCGACGCGAGGTTAT
GTTCCCGCCTTCTGTACACATGGCAGCAATCGATGGGGCTACCGCTGCATTGGAAAGTTTCTTGGATTTGGCGGAGCTTC
CCGATCATGCTGAAGTCCTCGGCCCTGTTGATCTGCCACCGGGTGTGAGTTTGCCTGGTGAATATGATGAGCAGCGCTTT
GGTCCGCCGCAGCGCCTTCTCATCAGAACTCCATTGGGACCGCGGTCTGAGTTGGGTAGAGCGCTGCGCTCAGCCCAGGT
GGCGCGTGCGGTGAGGAAGAATGATTTGCCGTTGCGTATTCAGATGGATCCGATTCACATCGGATAA

Upstream 100 bases:

>100_bases
GCACTGATTTGGACCTTCCTTGGGAGGCTATCGACCGCGTTGATGAACTTCGCGCAGCCCTCAAGTTGGCCTAAAAATCT
GATGTAGTATCTTCGGATTC

Downstream 100 bases:

>100_bases
AACTGCTGGTGAAAGGCCTAATGGGGTCGTGTCCCATGTGGGATATAAGATGGGGAGACAAGATCATCCACCCATAGGAA
TTATTGTTGAAGGAGCCTTC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MAKTRVPAPEKSVARVLPLLGLPHLDRLFDYRISEDQHDDVQPGVRVRVRFGGRLVDAIVMSRTAQTSHEGKLMWLDRVI
SPIVVYPPQTAKLIEQLSDRYGGVRSDLIRSALPARHAGAEEADTSTSWESLGEVKEPDLSSWSAYQHGQSFVDAVLAGT
TARASWQIAPGDDWALALASLAVKVVKDGGGALLVVPDQRDLDRLEAALRGLVAAKQITVLNSGLGPQARYRRFLSVLSG
QGRLIIGTRSAAFAPVKDLKLAVILNDGDDNLVDPRAPYAHAREVLTTRSSLEASSLIIAGHARTAETQLLVESGWMHNL
IAPRDTIRTRMPRIQAVGDSDFQMERDPMARSARLPGIAFHAVRSALERDQPALIQVPRKGYVPTLACGNCRTPARCRHC
NGPVGLPQGSSDLAGVPTCRWCGRPDSRFKCQNCGSPKLRAVVLGTERTAEELGRAFPSVRVITSGGNKVVDSVENRASI
VVSTPGAEPFVANSPERPEKSEKPEHKGAYGALLLLDTWALMGRQDLRAMEDALHKWAAAATLVHSHLHQGQVIVVADPS
FPAVQSLIRWDMAGAAAQELASRREVMFPPSVHMAAIDGATAALESFLDLAELPDHAEVLGPVDLPPGVSLPGEYDEQRF
GPPQRLLIRTPLGPRSELGRALRSAQVARAVRKNDLPLRIQMDPIHIG

Sequences:

>Translated_688_residues
MAKTRVPAPEKSVARVLPLLGLPHLDRLFDYRISEDQHDDVQPGVRVRVRFGGRLVDAIVMSRTAQTSHEGKLMWLDRVI
SPIVVYPPQTAKLIEQLSDRYGGVRSDLIRSALPARHAGAEEADTSTSWESLGEVKEPDLSSWSAYQHGQSFVDAVLAGT
TARASWQIAPGDDWALALASLAVKVVKDGGGALLVVPDQRDLDRLEAALRGLVAAKQITVLNSGLGPQARYRRFLSVLSG
QGRLIIGTRSAAFAPVKDLKLAVILNDGDDNLVDPRAPYAHAREVLTTRSSLEASSLIIAGHARTAETQLLVESGWMHNL
IAPRDTIRTRMPRIQAVGDSDFQMERDPMARSARLPGIAFHAVRSALERDQPALIQVPRKGYVPTLACGNCRTPARCRHC
NGPVGLPQGSSDLAGVPTCRWCGRPDSRFKCQNCGSPKLRAVVLGTERTAEELGRAFPSVRVITSGGNKVVDSVENRASI
VVSTPGAEPFVANSPERPEKSEKPEHKGAYGALLLLDTWALMGRQDLRAMEDALHKWAAAATLVHSHLHQGQVIVVADPS
FPAVQSLIRWDMAGAAAQELASRREVMFPPSVHMAAIDGATAALESFLDLAELPDHAEVLGPVDLPPGVSLPGEYDEQRF
GPPQRLLIRTPLGPRSELGRALRSAQVARAVRKNDLPLRIQMDPIHIG
>Mature_687_residues
AKTRVPAPEKSVARVLPLLGLPHLDRLFDYRISEDQHDDVQPGVRVRVRFGGRLVDAIVMSRTAQTSHEGKLMWLDRVIS
PIVVYPPQTAKLIEQLSDRYGGVRSDLIRSALPARHAGAEEADTSTSWESLGEVKEPDLSSWSAYQHGQSFVDAVLAGTT
ARASWQIAPGDDWALALASLAVKVVKDGGGALLVVPDQRDLDRLEAALRGLVAAKQITVLNSGLGPQARYRRFLSVLSGQ
GRLIIGTRSAAFAPVKDLKLAVILNDGDDNLVDPRAPYAHAREVLTTRSSLEASSLIIAGHARTAETQLLVESGWMHNLI
APRDTIRTRMPRIQAVGDSDFQMERDPMARSARLPGIAFHAVRSALERDQPALIQVPRKGYVPTLACGNCRTPARCRHCN
GPVGLPQGSSDLAGVPTCRWCGRPDSRFKCQNCGSPKLRAVVLGTERTAEELGRAFPSVRVITSGGNKVVDSVENRASIV
VSTPGAEPFVANSPERPEKSEKPEHKGAYGALLLLDTWALMGRQDLRAMEDALHKWAAAATLVHSHLHQGQVIVVADPSF
PAVQSLIRWDMAGAAAQELASRREVMFPPSVHMAAIDGATAALESFLDLAELPDHAEVLGPVDLPPGVSLPGEYDEQRFG
PPQRLLIRTPLGPRSELGRALRSAQVARAVRKNDLPLRIQMDPIHIG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=445, Percent_Identity=26.7415730337079, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 74593; Mature: 74462

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTRVPAPEKSVARVLPLLGLPHLDRLFDYRISEDQHDDVQPGVRVRVRFGGRLVDAIV
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHH
MSRTAQTSHEGKLMWLDRVISPIVVYPPQTAKLIEQLSDRYGGVRSDLIRSALPARHAGA
HHHHHCCCCCCCEEEHHHHHCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHCCC
EEADTSTSWESLGEVKEPDLSSWSAYQHGQSFVDAVLAGTTARASWQIAPGDDWALALAS
CCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHH
LAVKVVKDGGGALLVVPDQRDLDRLEAALRGLVAAKQITVLNSGLGPQARYRRFLSVLSG
HHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCC
QGRLIIGTRSAAFAPVKDLKLAVILNDGDDNLVDPRAPYAHAREVLTTRSSLEASSLIIA
CCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
GHARTAETQLLVESGWMHNLIAPRDTIRTRMPRIQAVGDSDFQMERDPMARSARLPGIAF
CCCCCCHHHEEECCCCHHHCCCCHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCCCHHH
HAVRSALERDQPALIQVPRKGYVPTLACGNCRTPARCRHCNGPVGLPQGSSDLAGVPTCR
HHHHHHHHCCCCCEEECCCCCCCCEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHH
WCGRPDSRFKCQNCGSPKLRAVVLGTERTAEELGRAFPSVRVITSGGNKVVDSVENRASI
CCCCCCCCCEECCCCCCCEEEEEECCHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCEE
VVSTPGAEPFVANSPERPEKSEKPEHKGAYGALLLLDTWALMGRQDLRAMEDALHKWAAA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ATLVHSHLHQGQVIVVADPSFPAVQSLIRWDMAGAAAQELASRREVMFPPSVHMAAIDGA
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCHH
TAALESFLDLAELPDHAEVLGPVDLPPGVSLPGEYDEQRFGPPQRLLIRTPLGPRSELGR
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHEEEECCCCCHHHHHH
ALRSAQVARAVRKNDLPLRIQMDPIHIG
HHHHHHHHHHHHHCCCCEEEEECCEECC
>Mature Secondary Structure 
AKTRVPAPEKSVARVLPLLGLPHLDRLFDYRISEDQHDDVQPGVRVRVRFGGRLVDAIV
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHH
MSRTAQTSHEGKLMWLDRVISPIVVYPPQTAKLIEQLSDRYGGVRSDLIRSALPARHAGA
HHHHHCCCCCCCEEEHHHHHCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHCCC
EEADTSTSWESLGEVKEPDLSSWSAYQHGQSFVDAVLAGTTARASWQIAPGDDWALALAS
CCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHH
LAVKVVKDGGGALLVVPDQRDLDRLEAALRGLVAAKQITVLNSGLGPQARYRRFLSVLSG
HHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCC
QGRLIIGTRSAAFAPVKDLKLAVILNDGDDNLVDPRAPYAHAREVLTTRSSLEASSLIIA
CCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
GHARTAETQLLVESGWMHNLIAPRDTIRTRMPRIQAVGDSDFQMERDPMARSARLPGIAF
CCCCCCHHHEEECCCCHHHCCCCHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCCCHHH
HAVRSALERDQPALIQVPRKGYVPTLACGNCRTPARCRHCNGPVGLPQGSSDLAGVPTCR
HHHHHHHHCCCCCEEECCCCCCCCEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHH
WCGRPDSRFKCQNCGSPKLRAVVLGTERTAEELGRAFPSVRVITSGGNKVVDSVENRASI
CCCCCCCCCEECCCCCCCEEEEEECCHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCEE
VVSTPGAEPFVANSPERPEKSEKPEHKGAYGALLLLDTWALMGRQDLRAMEDALHKWAAA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ATLVHSHLHQGQVIVVADPSFPAVQSLIRWDMAGAAAQELASRREVMFPPSVHMAAIDGA
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCHH
TAALESFLDLAELPDHAEVLGPVDLPPGVSLPGEYDEQRFGPPQRLLIRTPLGPRSELGR
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHEEEECCCCCHHHHHH
ALRSAQVARAVRKNDLPLRIQMDPIHIG
HHHHHHHHHHHHHCCCCEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]