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The map label for this gene is tyrS

Identifier: 62185046

GI number: 62185046

Start: 478968

End: 480206

Strand: Direct

Name: tyrS

Synonym: CAB417

Alternate gene names: 62185046

Gene position: 478968-480206 (Clockwise)

Preceding gene: 62185044

Following gene: 62185047

Centisome position: 41.85

GC content: 41.08

Gene sequence:

>1239_bases
ATGCGGGATTTTATACAACACTTGCAAGATCGGGGAATCCTCGAAAATTTTTCCTCAGGATTAGATAACGTAAGCACCCC
AGTTTCTGCCTATCTTGGATTTGATCCCACAGCCCCTTCTCTTCATATAGGACATTGGATAGGGATATGCTTTTTGCGTA
GGATGGCTCACTTTGGTCTCACTCCTGTGGCTCTTGTAGGTTCTGCGACCGGAATGATCGGAGACCCCTCAGGGAAAAGC
ATAGAACGTACCCTACTTGAGGAAAGCCAAGTCGCCTATAATAGCCAGAAACTCTCAGAATGTCTTGCTTACTATTTACC
CGGAGTGCAAATAGTAAATAACATAGACTGGTTGAAAGAAACGACTGTGATAGATTTCCTGAGAGACGTAGGGAAGCATT
TTAGACTAGGCGTTATGTTGGGTAAGGATACAGTCAAACAGCGTGTTCAGTCTGAAGAGGGCATTAGCTATACTGAATTT
AGCTACATGCTTTTGCAATCATATGATTTTGCTTATCTCTTTGAGAACTATGGAGTATGTTTGCAATGTGGGGGAAGCGA
TCAATGGGGGAATATTACTTCAGGCATTGATTATATCCGTCGTCATGGATTGGGACAGGCTTATGGTTTGACTTATCCGT
TGTTAACAAATAGCCAAGGTAAGAAGATTGGGAAGACTGAGTCAGGAACAATTTGGTTAGATCCCAAGCTAACATCTCCC
TATGAGTTATATCAGTATTTTTTAAGGCTTCCGGATCAAGAAGTGCCTAAAGTAGCACGTACATTAACTTTATTGAGTAA
TGAAGAAATCTTTGATCTAGACCAAAAGTTTTTATTAGATCCAGTAGCTGTGAAAAAGTTTGTTGCCGAAACTCTAGTCA
CCTCTATACACGGTGAAGACGGACTAAGAGAAGCGCAGGTAGTCACTCAAAGCATACATCCGGGTAAAGTATCTTCAGTT
TCTGAGAAGGATTTTCAAGATCTTGTTGCTATGGGCCAGGCAGTCTCTTTAGAAGGAACACAGGCACTCGGCAAACGGTG
GATAGATCTTTTTGTTGCATTAGGGGTTTGTAGTTCTAAAGGAGAAGCGAGAAGATTGATCGAGCAGAAAGGCCTCTATG
TAAATAGTGAACCTATTGAGAATGAACACAGTGTTTTTGAAGAAACTCAGGTGTGTTTCGATCAATATGTTTTATTAGCA
CAGGGAAAGAAGAAAAAACTCGTTTTGCGTCTAATTTAA

Upstream 100 bases:

>100_bases
CCGTTTATTTTTTTCTAGAGTAAATCCCTAAGGAATCTTGCATATAAGAGTACTGATCGTTAAGATAGTCCTTCAATTAT
TAAGATTTTTTGTTATTGCG

Downstream 100 bases:

>100_bases
GTGTGGAGGAGGATTGGTGGCAGATAAAGCTGGTGCGGATATTGGGCTAATTGGTTTAGCTGTAATGGGGAAAAATCTGG
TGTTGAATATGATCGACCAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MRDFIQHLQDRGILENFSSGLDNVSTPVSAYLGFDPTAPSLHIGHWIGICFLRRMAHFGLTPVALVGSATGMIGDPSGKS
IERTLLEESQVAYNSQKLSECLAYYLPGVQIVNNIDWLKETTVIDFLRDVGKHFRLGVMLGKDTVKQRVQSEEGISYTEF
SYMLLQSYDFAYLFENYGVCLQCGGSDQWGNITSGIDYIRRHGLGQAYGLTYPLLTNSQGKKIGKTESGTIWLDPKLTSP
YELYQYFLRLPDQEVPKVARTLTLLSNEEIFDLDQKFLLDPVAVKKFVAETLVTSIHGEDGLREAQVVTQSIHPGKVSSV
SEKDFQDLVAMGQAVSLEGTQALGKRWIDLFVALGVCSSKGEARRLIEQKGLYVNSEPIENEHSVFEETQVCFDQYVLLA
QGKKKKLVLRLI

Sequences:

>Translated_412_residues
MRDFIQHLQDRGILENFSSGLDNVSTPVSAYLGFDPTAPSLHIGHWIGICFLRRMAHFGLTPVALVGSATGMIGDPSGKS
IERTLLEESQVAYNSQKLSECLAYYLPGVQIVNNIDWLKETTVIDFLRDVGKHFRLGVMLGKDTVKQRVQSEEGISYTEF
SYMLLQSYDFAYLFENYGVCLQCGGSDQWGNITSGIDYIRRHGLGQAYGLTYPLLTNSQGKKIGKTESGTIWLDPKLTSP
YELYQYFLRLPDQEVPKVARTLTLLSNEEIFDLDQKFLLDPVAVKKFVAETLVTSIHGEDGLREAQVVTQSIHPGKVSSV
SEKDFQDLVAMGQAVSLEGTQALGKRWIDLFVALGVCSSKGEARRLIEQKGLYVNSEPIENEHSVFEETQVCFDQYVLLA
QGKKKKLVLRLI
>Mature_412_residues
MRDFIQHLQDRGILENFSSGLDNVSTPVSAYLGFDPTAPSLHIGHWIGICFLRRMAHFGLTPVALVGSATGMIGDPSGKS
IERTLLEESQVAYNSQKLSECLAYYLPGVQIVNNIDWLKETTVIDFLRDVGKHFRLGVMLGKDTVKQRVQSEEGISYTEF
SYMLLQSYDFAYLFENYGVCLQCGGSDQWGNITSGIDYIRRHGLGQAYGLTYPLLTNSQGKKIGKTESGTIWLDPKLTSP
YELYQYFLRLPDQEVPKVARTLTLLSNEEIFDLDQKFLLDPVAVKKFVAETLVTSIHGEDGLREAQVVTQSIHPGKVSSV
SEKDFQDLVAMGQAVSLEGTQALGKRWIDLFVALGVCSSKGEARRLIEQKGLYVNSEPIENEHSVFEETQVCFDQYVLLA
QGKKKKLVLRLI

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=407, Percent_Identity=33.6609336609337, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1787925, Length=418, Percent_Identity=41.3875598086124, Blast_Score=304, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI25145802, Length=416, Percent_Identity=35.0961538461538, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI25145805, Length=325, Percent_Identity=32.9230769230769, Blast_Score=141, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6325160, Length=443, Percent_Identity=29.7968397291196, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI20130383, Length=404, Percent_Identity=34.6534653465347, Blast_Score=251, Evalue=4e-67,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_CHLAB (Q5L661)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219831.1
- ProteinModelPortal:   Q5L661
- SMR:   Q5L661
- GeneID:   3338237
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB417
- HOGENOM:   HBG288125
- OMA:   HIRENIT
- PhylomeDB:   Q5L661
- ProtClustDB:   PRK05912
- BioCyc:   CABO218497:CAB417-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46085; Mature: 46085

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 31-31 BINDING 162-162 BINDING 166-166 BINDING 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDFIQHLQDRGILENFSSGLDNVSTPVSAYLGFDPTAPSLHIGHWIGICFLRRMAHFGL
CHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCC
TPVALVGSATGMIGDPSGKSIERTLLEESQVAYNSQKLSECLAYYLPGVQIVNNIDWLKE
CHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TTVIDFLRDVGKHFRLGVMLGKDTVKQRVQSEEGISYTEFSYMLLQSYDFAYLFENYGVC
HHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCEE
LQCGGSDQWGNITSGIDYIRRHGLGQAYGLTYPLLTNSQGKKIGKTESGTIWLDPKLTSP
EEECCCCCCCCHHHHHHHHHHCCCCCHHCCEECEEECCCCCCCCCCCCCEEEECCCCCCH
YELYQYFLRLPDQEVPKVARTLTLLSNEEIFDLDQKFLLDPVAVKKFVAETLVTSIHGED
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHCCHHHHHCHHHHHHHHHHHHHHHHCCCC
GLREAQVVTQSIHPGKVSSVSEKDFQDLVAMGQAVSLEGTQALGKRWIDLFVALGVCSSK
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GEARRLIEQKGLYVNSEPIENEHSVFEETQVCFDQYVLLAQGKKKKLVLRLI
HHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MRDFIQHLQDRGILENFSSGLDNVSTPVSAYLGFDPTAPSLHIGHWIGICFLRRMAHFGL
CHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCC
TPVALVGSATGMIGDPSGKSIERTLLEESQVAYNSQKLSECLAYYLPGVQIVNNIDWLKE
CHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TTVIDFLRDVGKHFRLGVMLGKDTVKQRVQSEEGISYTEFSYMLLQSYDFAYLFENYGVC
HHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCEE
LQCGGSDQWGNITSGIDYIRRHGLGQAYGLTYPLLTNSQGKKIGKTESGTIWLDPKLTSP
EEECCCCCCCCHHHHHHHHHHCCCCCHHCCEECEEECCCCCCCCCCCCCEEEECCCCCCH
YELYQYFLRLPDQEVPKVARTLTLLSNEEIFDLDQKFLLDPVAVKKFVAETLVTSIHGED
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHCCHHHHHCHHHHHHHHHHHHHHHHCCCC
GLREAQVVTQSIHPGKVSSVSEKDFQDLVAMGQAVSLEGTQALGKRWIDLFVALGVCSSK
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GEARRLIEQKGLYVNSEPIENEHSVFEETQVCFDQYVLLAQGKKKKLVLRLI
HHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA