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The map label for this gene is murE

Identifier: 62184995

GI number: 62184995

Start: 419373

End: 420824

Strand: Direct

Name: murE

Synonym: CAB363

Alternate gene names: 62184995

Gene position: 419373-420824 (Clockwise)

Preceding gene: 62184994

Following gene: 62184996

Centisome position: 36.65

GC content: 41.46

Gene sequence:

>1452_bases
ATGAATTTAAAAGAACTCCTTCACAATACCAAAGCGAAAATTTATGGGAAGATTTCTTCTGTAGAGGTAAGAAATCTCAC
AAGAGATTCTCGCAATGTTGGAGTTGGAGATATCTTTATCGCCAAGCAAGGCAAACACTGTGATGGTAATGACTTTTCCC
ACCTAGCTGTTGAAAATGGAGCGATTGCTGTAGCTTCTTCTATCTATAATCCTTTTTTACCCGTTGTACAAATTATTTCT
TCTGATCTTCCTCGGCTTGAGGCGGATCTGGCTGCGAAATACTACGGTCATCCTTCGCAAAAGCTCTGTGTTGTGGGGAT
TACAGGCACCAATGGGAAAACGACAGTTTCCCATTTAATCAAACTTCTGTTTGATGCTTGCGACAAGCCTGCAGGTTTAA
TAGGGACGATTGAGCATATTCTGGGAAATAGTCGTATTCAAGATGGGTATACCACTCCTGAATCCTGTTTGTTGCAAAAG
TATTTAGCTAAGATGGTGAAGAGCCATCTTTCGGCTGCGGTTATGGAAGTCTCTTCTATCGGTCTGGCTGTAAACAGATT
GGCTAACGTCGATTTTGATGTTGGTGTCTTAACTAACCTGACTCTAGACCATTTAGATTTTCATTCTTCGTTTGAAGAGT
ATAAGCAAGCAAAGCTGAAGTTATTTTCGATGCTTCCTTCTTCAGGCCTCGCTGTAGTCAACAACGACTTATGTGATGCT
GCGCAGTTTATTGAGGCTACTCAAGCCCAACCTATTACCTATGGAATAGAGCAGCATGCAGATTATCAAGCTTCCCATGT
GCGCTTTTCTCCTTTCGGAACGGATTTTGACTTGCTATATAAGGGAGAAACTTTTGCCTGTTATTCACCTTTAATCGGAC
AGCATAACATTTATAATGTTCTTGCGGCTATTGCTGTCACTCACCAACGATTACGTTGTGATTTACCCCACCTCATCTCT
GTTATTGCCAATGTAGGAGCCCCTAGAGGGCGGCTAGAACCCATATTTTCCGGCCCTTGTCCTATTTACATTGATTATGC
TCATACTCCCGATGCCTTAGATAATGTATGCCAAACACTCCAAGCTCTCCTTCCTCAAGATGGCAGACTTATCGTGGTCT
TTGGGTGCGGAGGTGATCGAGATCAGAGCAAAAGAAAAATCATGGCTCAAGTCGTCGAGAAGTATGGATTTGCTGTTGTG
ACTACAGACAATCCTCGTGGTGAAGATCCAGAAAAGATCATTAATGAAATTTGTTCCGGCTTTTTAAAAAGAAATTTTTC
TATCGAAATCGACAGAAAACAAGCAATTACATATGCTTTGTCCATTGCCTCAGATAGGGATATAGTGTTAGTGGCAGGTA
AAGGGCATGAGACGTACCAGATTTTTAAACATCAAACCATCGCTTTTGATGATAAGGAAATCGTACTCGGGGTATTGTCG
TCTTATGTTTAA

Upstream 100 bases:

>100_bases
ATTCTTTGCCGAATGAAAATAAGAGTGCTTACAGATATATTAGGAACAATTTTGGATATAGAAGTGGTCTAGCTTTTGAA
GAAGGACCTCAGGCTAAAAA

Downstream 100 bases:

>100_bases
TCGTTACACTTTACTCACTGCCTGTGTCTTCGGAACTGCTTTAGGAGGAATTGCAGCTGAAAGTGCACTTCCACAGCGTG
TGCGTCGTAACGAAGTCATT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNLKELLHNTKAKIYGKISSVEVRNLTRDSRNVGVGDIFIAKQGKHCDGNDFSHLAVENGAIAVASSIYNPFLPVVQIIS
SDLPRLEADLAAKYYGHPSQKLCVVGITGTNGKTTVSHLIKLLFDACDKPAGLIGTIEHILGNSRIQDGYTTPESCLLQK
YLAKMVKSHLSAAVMEVSSIGLAVNRLANVDFDVGVLTNLTLDHLDFHSSFEEYKQAKLKLFSMLPSSGLAVVNNDLCDA
AQFIEATQAQPITYGIEQHADYQASHVRFSPFGTDFDLLYKGETFACYSPLIGQHNIYNVLAAIAVTHQRLRCDLPHLIS
VIANVGAPRGRLEPIFSGPCPIYIDYAHTPDALDNVCQTLQALLPQDGRLIVVFGCGGDRDQSKRKIMAQVVEKYGFAVV
TTDNPRGEDPEKIINEICSGFLKRNFSIEIDRKQAITYALSIASDRDIVLVAGKGHETYQIFKHQTIAFDDKEIVLGVLS
SYV

Sequences:

>Translated_483_residues
MNLKELLHNTKAKIYGKISSVEVRNLTRDSRNVGVGDIFIAKQGKHCDGNDFSHLAVENGAIAVASSIYNPFLPVVQIIS
SDLPRLEADLAAKYYGHPSQKLCVVGITGTNGKTTVSHLIKLLFDACDKPAGLIGTIEHILGNSRIQDGYTTPESCLLQK
YLAKMVKSHLSAAVMEVSSIGLAVNRLANVDFDVGVLTNLTLDHLDFHSSFEEYKQAKLKLFSMLPSSGLAVVNNDLCDA
AQFIEATQAQPITYGIEQHADYQASHVRFSPFGTDFDLLYKGETFACYSPLIGQHNIYNVLAAIAVTHQRLRCDLPHLIS
VIANVGAPRGRLEPIFSGPCPIYIDYAHTPDALDNVCQTLQALLPQDGRLIVVFGCGGDRDQSKRKIMAQVVEKYGFAVV
TTDNPRGEDPEKIINEICSGFLKRNFSIEIDRKQAITYALSIASDRDIVLVAGKGHETYQIFKHQTIAFDDKEIVLGVLS
SYV
>Mature_483_residues
MNLKELLHNTKAKIYGKISSVEVRNLTRDSRNVGVGDIFIAKQGKHCDGNDFSHLAVENGAIAVASSIYNPFLPVVQIIS
SDLPRLEADLAAKYYGHPSQKLCVVGITGTNGKTTVSHLIKLLFDACDKPAGLIGTIEHILGNSRIQDGYTTPESCLLQK
YLAKMVKSHLSAAVMEVSSIGLAVNRLANVDFDVGVLTNLTLDHLDFHSSFEEYKQAKLKLFSMLPSSGLAVVNNDLCDA
AQFIEATQAQPITYGIEQHADYQASHVRFSPFGTDFDLLYKGETFACYSPLIGQHNIYNVLAAIAVTHQRLRCDLPHLIS
VIANVGAPRGRLEPIFSGPCPIYIDYAHTPDALDNVCQTLQALLPQDGRLIVVFGCGGDRDQSKRKIMAQVVEKYGFAVV
TTDNPRGEDPEKIINEICSGFLKRNFSIEIDRKQAITYALSIASDRDIVLVAGKGHETYQIFKHQTIAFDDKEIVLGVLS
SYV

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=507, Percent_Identity=33.9250493096647, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786274, Length=361, Percent_Identity=25.207756232687, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_CHLAB (Q5L6B2)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219780.1
- HSSP:   P22188
- ProteinModelPortal:   Q5L6B2
- SMR:   Q5L6B2
- GeneID:   3337461
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB363
- HOGENOM:   HBG602753
- OMA:   IGTIANY
- PhylomeDB:   Q5L6B2
- ProtClustDB:   PRK00139
- BioCyc:   CABO218497:CAB363-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 52985; Mature: 52985

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 178-178 BINDING 186-186 BINDING 380-380 BINDING 453-453 BINDING 457-457

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKELLHNTKAKIYGKISSVEVRNLTRDSRNVGVGDIFIAKQGKHCDGNDFSHLAVENG
CCHHHHHHCCHHHEEECCCCEEHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEEECCC
AIAVASSIYNPFLPVVQIISSDLPRLEADLAAKYYGHPSQKLCVVGITGTNGKTTVSHLI
CEEEHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
KLLFDACDKPAGLIGTIEHILGNSRIQDGYTTPESCLLQKYLAKMVKSHLSAAVMEVSSI
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAVNRLANVDFDVGVLTNLTLDHLDFHSSFEEYKQAKLKLFSMLPSSGLAVVNNDLCDA
HHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHH
AQFIEATQAQPITYGIEQHADYQASHVRFSPFGTDFDLLYKGETFACYSPLIGQHNIYNV
HHHHHHHCCCCEEECHHHCCCCCCCCEEECCCCCCEEEEECCCEEEECCCCCCCCHHHHH
LAAIAVTHQRLRCDLPHLISVIANVGAPRGRLEPIFSGPCPIYIDYAHTPDALDNVCQTL
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
QALLPQDGRLIVVFGCGGDRDQSKRKIMAQVVEKYGFAVVTTDNPRGEDPEKIINEICSG
HHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHH
FLKRNFSIEIDRKQAITYALSIASDRDIVLVAGKGHETYQIFKHQTIAFDDKEIVLGVLS
HHHCCCEEEECHHHHEEEEEEECCCCCEEEEECCCCCEEEHHHCCEEEECCHHHHHHHHH
SYV
HCC
>Mature Secondary Structure
MNLKELLHNTKAKIYGKISSVEVRNLTRDSRNVGVGDIFIAKQGKHCDGNDFSHLAVENG
CCHHHHHHCCHHHEEECCCCEEHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEEECCC
AIAVASSIYNPFLPVVQIISSDLPRLEADLAAKYYGHPSQKLCVVGITGTNGKTTVSHLI
CEEEHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
KLLFDACDKPAGLIGTIEHILGNSRIQDGYTTPESCLLQKYLAKMVKSHLSAAVMEVSSI
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAVNRLANVDFDVGVLTNLTLDHLDFHSSFEEYKQAKLKLFSMLPSSGLAVVNNDLCDA
HHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHH
AQFIEATQAQPITYGIEQHADYQASHVRFSPFGTDFDLLYKGETFACYSPLIGQHNIYNV
HHHHHHHCCCCEEECHHHCCCCCCCCEEECCCCCCEEEEECCCEEEECCCCCCCCHHHHH
LAAIAVTHQRLRCDLPHLISVIANVGAPRGRLEPIFSGPCPIYIDYAHTPDALDNVCQTL
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
QALLPQDGRLIVVFGCGGDRDQSKRKIMAQVVEKYGFAVVTTDNPRGEDPEKIINEICSG
HHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHH
FLKRNFSIEIDRKQAITYALSIASDRDIVLVAGKGHETYQIFKHQTIAFDDKEIVLGVLS
HHHCCCEEEECHHHHEEEEEEECCCCCEEEEECCCCCEEEHHHCCEEEECCHHHHHHHHH
SYV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA