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The map label for this gene is amiC [H]

Identifier: 62184996

GI number: 62184996

Start: 420817

End: 421536

Strand: Direct

Name: amiC [H]

Synonym: CAB364

Alternate gene names: 62184996

Gene position: 420817-421536 (Clockwise)

Preceding gene: 62184995

Following gene: 62184997

Centisome position: 36.77

GC content: 42.5

Gene sequence:

>720_bases
ATGTTTAATCGTTACACTTTACTCACTGCCTGTGTCTTCGGAACTGCTTTAGGAGGAATTGCAGCTGAAAGTGCACTTCC
ACAGCGTGTGCGTCGTAACGAAGTCATTTTTATCGACCCGGGACACGGAGGTAAAGATCAAGGCACTGCAAGTAAAGAGT
TCCATTATGAGGAAAAATCTCTAACCCTATCTCTTGCGTTTTCAGTGCAGAGTTATCTCAAGAGAATGGGGTATAAGCCA
GTTCTTACGCGAACATCTGATGTGTATGTAGATCTCGGGAAAAGAGCGGCTTTAGCAAATCAAAACAAAGCTGATATTTT
TGTCAGTATTCACTGTAATTATTCGTCCAACACGTCAGCCTTTGGTACTGAAGTATATTTTTATAATGGCAAAAATAACG
TTGCTTCGAGAAGTCGTGCTTCGGAAGCTCTAGCTAAGGATGTCCTAAATGCTATGCAGAAAAATGGCGCATTGAAAATT
CGGGGAGCGAAGAATGGCAATTTCGCTGTTATCCGAGAAACCACAATGCCTGCTATTTTAATTGAAACAGGATTCCTTTC
TAACCCCAGAGAACGAGCTGCTCTTTCCGATGCACGTTACCGAATGCATATAGCCAAAGGCATAGCCGAGGGCGTGCATA
CATTTCTTACTGGTCCGAACTTTCAGAAACCAAGTTTAGCAAGTGTAAAGGCCAGAAAACTTTCTGCAAAGGTGAATTAG

Upstream 100 bases:

>100_bases
GTGTTAGTGGCAGGTAAAGGGCATGAGACGTACCAGATTTTTAAACATCAAACCATCGCTTTTGATGATAAGGAAATCGT
ACTCGGGGTATTGTCGTCTT

Downstream 100 bases:

>100_bases
TTTTTTTAATTAGAAAAAAGCCGTCCTCGATGCGATTCGAACGCATGACCTGCTGCTTAGGAGGCAACCGCTCTATCCTA
CTGAGCTACGAGGACACTAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MFNRYTLLTACVFGTALGGIAAESALPQRVRRNEVIFIDPGHGGKDQGTASKEFHYEEKSLTLSLAFSVQSYLKRMGYKP
VLTRTSDVYVDLGKRAALANQNKADIFVSIHCNYSSNTSAFGTEVYFYNGKNNVASRSRASEALAKDVLNAMQKNGALKI
RGAKNGNFAVIRETTMPAILIETGFLSNPRERAALSDARYRMHIAKGIAEGVHTFLTGPNFQKPSLASVKARKLSAKVN

Sequences:

>Translated_239_residues
MFNRYTLLTACVFGTALGGIAAESALPQRVRRNEVIFIDPGHGGKDQGTASKEFHYEEKSLTLSLAFSVQSYLKRMGYKP
VLTRTSDVYVDLGKRAALANQNKADIFVSIHCNYSSNTSAFGTEVYFYNGKNNVASRSRASEALAKDVLNAMQKNGALKI
RGAKNGNFAVIRETTMPAILIETGFLSNPRERAALSDARYRMHIAKGIAEGVHTFLTGPNFQKPSLASVKARKLSAKVN
>Mature_239_residues
MFNRYTLLTACVFGTALGGIAAESALPQRVRRNEVIFIDPGHGGKDQGTASKEFHYEEKSLTLSLAFSVQSYLKRMGYKP
VLTRTSDVYVDLGKRAALANQNKADIFVSIHCNYSSNTSAFGTEVYFYNGKNNVASRSRASEALAKDVLNAMQKNGALKI
RGAKNGNFAVIRETTMPAILIETGFLSNPRERAALSDARYRMHIAKGIAEGVHTFLTGPNFQKPSLASVKARKLSAKVN

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=227, Percent_Identity=28.1938325991189, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87082163, Length=223, Percent_Identity=27.8026905829596, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26132; Mature: 26132

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNRYTLLTACVFGTALGGIAAESALPQRVRRNEVIFIDPGHGGKDQGTASKEFHYEEKS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCE
LTLSLAFSVQSYLKRMGYKPVLTRTSDVYVDLGKRAALANQNKADIFVSIHCNYSSNTSA
EEEEEHHHHHHHHHHCCCCCEEECCCEEEEECCCCHHHCCCCCCEEEEEEEECCCCCCCC
FGTEVYFYNGKNNVASRSRASEALAKDVLNAMQKNGALKIRGAKNGNFAVIRETTMPAIL
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCEEE
IETGFLSNPRERAALSDARYRMHIAKGIAEGVHTFLTGPNFQKPSLASVKARKLSAKVN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEECCC
>Mature Secondary Structure
MFNRYTLLTACVFGTALGGIAAESALPQRVRRNEVIFIDPGHGGKDQGTASKEFHYEEKS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCE
LTLSLAFSVQSYLKRMGYKPVLTRTSDVYVDLGKRAALANQNKADIFVSIHCNYSSNTSA
EEEEEHHHHHHHHHHCCCCCEEECCCEEEEECCCCHHHCCCCCCEEEEEEEECCCCCCCC
FGTEVYFYNGKNNVASRSRASEALAKDVLNAMQKNGALKIRGAKNGNFAVIRETTMPAIL
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCEEE
IETGFLSNPRERAALSDARYRMHIAKGIAEGVHTFLTGPNFQKPSLASVKARKLSAKVN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]