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The map label for this gene is glyA

Identifier: 62184863

GI number: 62184863

Start: 240926

End: 242419

Strand: Direct

Name: glyA

Synonym: CAB220

Alternate gene names: 62184863

Gene position: 240926-242419 (Clockwise)

Preceding gene: 62184858

Following gene: 62184864

Centisome position: 21.05

GC content: 41.63

Gene sequence:

>1494_bases
ATGGTTTCATTGTTGCATAAATTTTTAGAAAATGCTTCGGGGAAGAAGGGGCAGGATCTAGCTTCTACCGCATACTTAGC
TGCATTAGATCATCTTTTACATTCTTTTCCTTCGATTGGGAAAAGCATTATCGATGAATTGAAGAGTCAGCGTTCACGCT
TAAAGATGATTGCTTCTGAGAATTATGCTTCTATTTCAGTTCAGCTTGCTATGGGGAACTTGCTTACAGATAAGTATTGC
GAGGGGAGCCCCTTTAAGCGGTTCTATTCTTGTTGTGAGAATGTAGATGCTATCGAGTGGGAATGTGTGGAAACGGCTAA
AGAACTTTTCGGAGCGGAAAGTGCTTTTGTACAACCACATTCGGGTGCAGATGCGAATTTATTAGCCATCATGGCAATTA
TTACGCAGAAAATTCAAGGGCCTGCCGTTAAGCGTTTGGGATATAAAACGATTAATGATCTTACGGATAAAGAATATACG
GAATTAAAAGCTGAGATAGGTTCTCACGTCTGTTTAGGACCTTCGCTGAACTCCGGAGGGCATTTAACCCATGGAACTGT
ACGTTTAAATATCATGTCCAAGTTGATGCGTTGTGTGCCTTATGAAGTGAATAAAAAGACGGAGTGTTTCGATTATTCGG
AGATCGCACGTTTAGTACGAACATATAAACCTACAGTACTCATTGCTGGGTATTCTTCATATTCCAGAAGATTGAATTTT
TCCACCCTAAAACAAATAGCTGATGATTGTGGAGCTGTTTTATGGGTAGATATGGCGCATTTTGCTGGTCTGGTTGCTGG
CGGTGTATTTATCGAAGAGGAAAATCCGATTCCGTTTGCCGACATTATTACTACGACAACTCATAAGACTTTGCGAGGAC
CTCGTGGAGGTTTAGTTTTAGCATCCAAAGAATATGATGCAGTGATTAATCGCGCTTGTCCTTTGATGATGGGAGGGCCT
TTGCCGCATGTTATTGCAGCCAAGGCTGTTGCGTTAAAAGAAGCGCTAACTGTCGACTTTAAAAAGTACGCGCATCAGGT
TGTGGACAACGCGAGAACTTTAGCTGAGCATTTTCAAAAACAGGGATTACGCTTGCTTACTGGCGGTACAGATAACCACA
TGTTAATTATAGATCTAACTTCTCTAGGGATATCTGGACGCATTGCTGAGGACATATTAAGTTCTGTAGGTATTGCTGTG
AATCGCAATACCATACCATCGGATGCTGTAGGGAAGTGGGATACCTCAGGGATACGTTTGGGAACACCTGCTTTAACAAC
TCTCGGTATGGGCAGTGATGAGATGGAAGAAGTTGCGAATATTATTGTGAAAGTATTGCGAAATATTACTTTGAGACGTA
ATGCTGATGATAGTTTTAGTAAAAGTGAAGGAGAGCTTCCAGAAAACATTGCTGAAGAAGCAAGAGCTCGAGTCGCAGGC
TTATTATCGCGGTTCCCGCTTTATCCTGAAATCGATCTGGAAACTTTAGTTTAG

Upstream 100 bases:

>100_bases
AAAAATCGTAAGAGTTTTTGACTTTTCTTAGGTTAGACGCAGAGAGCTTCGCTTTGTATTATAGTTTTTCATTCGTAGAG
TTGATAATAGAGGTTCGGCA

Downstream 100 bases:

>100_bases
TTAGGAGATATGTTGTTCTATGGCTGATGGGGAAGTAGAGAATAAGTTACGAGATGTTATAGAGAGAAAAATCTTAGATG
CCCGTCGGGTATTTTTTTCT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MVSLLHKFLENASGKKGQDLASTAYLAALDHLLHSFPSIGKSIIDELKSQRSRLKMIASENYASISVQLAMGNLLTDKYC
EGSPFKRFYSCCENVDAIEWECVETAKELFGAESAFVQPHSGADANLLAIMAIITQKIQGPAVKRLGYKTINDLTDKEYT
ELKAEIGSHVCLGPSLNSGGHLTHGTVRLNIMSKLMRCVPYEVNKKTECFDYSEIARLVRTYKPTVLIAGYSSYSRRLNF
STLKQIADDCGAVLWVDMAHFAGLVAGGVFIEEENPIPFADIITTTTHKTLRGPRGGLVLASKEYDAVINRACPLMMGGP
LPHVIAAKAVALKEALTVDFKKYAHQVVDNARTLAEHFQKQGLRLLTGGTDNHMLIIDLTSLGISGRIAEDILSSVGIAV
NRNTIPSDAVGKWDTSGIRLGTPALTTLGMGSDEMEEVANIIVKVLRNITLRRNADDSFSKSEGELPENIAEEARARVAG
LLSRFPLYPEIDLETLV

Sequences:

>Translated_497_residues
MVSLLHKFLENASGKKGQDLASTAYLAALDHLLHSFPSIGKSIIDELKSQRSRLKMIASENYASISVQLAMGNLLTDKYC
EGSPFKRFYSCCENVDAIEWECVETAKELFGAESAFVQPHSGADANLLAIMAIITQKIQGPAVKRLGYKTINDLTDKEYT
ELKAEIGSHVCLGPSLNSGGHLTHGTVRLNIMSKLMRCVPYEVNKKTECFDYSEIARLVRTYKPTVLIAGYSSYSRRLNF
STLKQIADDCGAVLWVDMAHFAGLVAGGVFIEEENPIPFADIITTTTHKTLRGPRGGLVLASKEYDAVINRACPLMMGGP
LPHVIAAKAVALKEALTVDFKKYAHQVVDNARTLAEHFQKQGLRLLTGGTDNHMLIIDLTSLGISGRIAEDILSSVGIAV
NRNTIPSDAVGKWDTSGIRLGTPALTTLGMGSDEMEEVANIIVKVLRNITLRRNADDSFSKSEGELPENIAEEARARVAG
LLSRFPLYPEIDLETLV
>Mature_497_residues
MVSLLHKFLENASGKKGQDLASTAYLAALDHLLHSFPSIGKSIIDELKSQRSRLKMIASENYASISVQLAMGNLLTDKYC
EGSPFKRFYSCCENVDAIEWECVETAKELFGAESAFVQPHSGADANLLAIMAIITQKIQGPAVKRLGYKTINDLTDKEYT
ELKAEIGSHVCLGPSLNSGGHLTHGTVRLNIMSKLMRCVPYEVNKKTECFDYSEIARLVRTYKPTVLIAGYSSYSRRLNF
STLKQIADDCGAVLWVDMAHFAGLVAGGVFIEEENPIPFADIITTTTHKTLRGPRGGLVLASKEYDAVINRACPLMMGGP
LPHVIAAKAVALKEALTVDFKKYAHQVVDNARTLAEHFQKQGLRLLTGGTDNHMLIIDLTSLGISGRIAEDILSSVGIAV
NRNTIPSDAVGKWDTSGIRLGTPALTTLGMGSDEMEEVANIIVKVLRNITLRRNADDSFSKSEGELPENIAEEARARVAG
LLSRFPLYPEIDLETLV

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=495, Percent_Identity=35.959595959596, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI261862350, Length=495, Percent_Identity=35.959595959596, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI261862348, Length=495, Percent_Identity=35.959595959596, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI19923315, Length=493, Percent_Identity=35.6997971602434, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI22547186, Length=479, Percent_Identity=36.3256784968685, Blast_Score=249, Evalue=5e-66,
Organism=Homo sapiens, GI261862346, Length=493, Percent_Identity=34.8884381338742, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI22547189, Length=463, Percent_Identity=35.4211663066955, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1788902, Length=443, Percent_Identity=40.8577878103837, Blast_Score=302, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI25144729, Length=490, Percent_Identity=35.1020408163265, Blast_Score=278, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI25144732, Length=490, Percent_Identity=35.1020408163265, Blast_Score=277, Evalue=8e-75,
Organism=Saccharomyces cerevisiae, GI6319739, Length=440, Percent_Identity=37.0454545454545, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6323087, Length=440, Percent_Identity=35.6818181818182, Blast_Score=263, Evalue=3e-71,
Organism=Drosophila melanogaster, GI221329721, Length=495, Percent_Identity=35.959595959596, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24640005, Length=495, Percent_Identity=36.1616161616162, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_CHLAB (Q5L6P4)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219648.1
- ProteinModelPortal:   Q5L6P4
- GeneID:   3337735
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB220
- HOGENOM:   HBG301263
- OMA:   GRAYVTC
- ProtClustDB:   PRK13580
- BioCyc:   CABO218497:CAB220-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 54260; Mature: 54260

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLLHKFLENASGKKGQDLASTAYLAALDHLLHSFPSIGKSIIDELKSQRSRLKMIASE
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NYASISVQLAMGNLLTDKYCEGSPFKRFYSCCENVDAIEWECVETAKELFGAESAFVQPH
CCCEEEEEEECCHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEECCC
SGADANLLAIMAIITQKIQGPAVKRLGYKTINDLTDKEYTELKAEIGSHVCLGPSLNSGG
CCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHCCCHHHHHHHHHHCCCEEECCCCCCCC
HLTHGTVRLNIMSKLMRCVPYEVNKKTECFDYSEIARLVRTYKPTVLIAGYSSYSRRLNF
CEEECEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECHHHHHCCCH
STLKQIADDCGAVLWVDMAHFAGLVAGGVFIEEENPIPFADIITTTTHKTLRGPRGGLVL
HHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEE
ASKEYDAVINRACPLMMGGPLPHVIAAKAVALKEALTVDFKKYAHQVVDNARTLAEHFQK
ECCHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLRLLTGGTDNHMLIIDLTSLGISGRIAEDILSSVGIAVNRNTIPSDAVGKWDTSGIRL
CCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEE
GTPALTTLGMGSDEMEEVANIIVKVLRNITLRRNADDSFSKSEGELPENIAEEARARVAG
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLSRFPLYPEIDLETLV
HHHHCCCCCCCCHHHCC
>Mature Secondary Structure
MVSLLHKFLENASGKKGQDLASTAYLAALDHLLHSFPSIGKSIIDELKSQRSRLKMIASE
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NYASISVQLAMGNLLTDKYCEGSPFKRFYSCCENVDAIEWECVETAKELFGAESAFVQPH
CCCEEEEEEECCHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEECCC
SGADANLLAIMAIITQKIQGPAVKRLGYKTINDLTDKEYTELKAEIGSHVCLGPSLNSGG
CCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHCCCHHHHHHHHHHCCCEEECCCCCCCC
HLTHGTVRLNIMSKLMRCVPYEVNKKTECFDYSEIARLVRTYKPTVLIAGYSSYSRRLNF
CEEECEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECHHHHHCCCH
STLKQIADDCGAVLWVDMAHFAGLVAGGVFIEEENPIPFADIITTTTHKTLRGPRGGLVL
HHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEE
ASKEYDAVINRACPLMMGGPLPHVIAAKAVALKEALTVDFKKYAHQVVDNARTLAEHFQK
ECCHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLRLLTGGTDNHMLIIDLTSLGISGRIAEDILSSVGIAVNRNTIPSDAVGKWDTSGIRL
CCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEE
GTPALTTLGMGSDEMEEVANIIVKVLRNITLRRNADDSFSKSEGELPENIAEEARARVAG
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLSRFPLYPEIDLETLV
HHHHCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA