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The map label for this gene is thiE

Identifier: 62184842

GI number: 62184842

Start: 215725

End: 216363

Strand: Direct

Name: thiE

Synonym: CAB198

Alternate gene names: 62184842

Gene position: 215725-216363 (Clockwise)

Preceding gene: 62184838

Following gene: 62184843

Centisome position: 18.85

GC content: 43.35

Gene sequence:

>639_bases
TTGGAAGAAAACTTTTTCAAACTGATTCTCATCACCAATAAACAACAGATTTCGGTAGAGGAGTATCTCGATTTCGTTTG
TGCTTGTGTACAATCTGGAGTGACCTCGGTGCAATTGCGTGAAAAGGAACTTTCATACAGAGAGTTGTTGGGTTTTGGAG
AAGCATTAAAGTCTATTTTAGATCCTTTGGAGATACCTTTAATTGTCAGTGACAGCGTGTCGGTGTGTTTAGATTTGGAT
GCTACAGGTGTCCACTTAGGACAGACAGACGGTGATGTTATCGAGGCTAGGGAGCTTCTAGGTTCTGATAAAATCATCGG
GTGGAATGTCAATACCCTGGATCAACTGCTCAATGCGAATACTCTGCCTATTGATTATTTGGGATTGAGTGCCATGTTTG
CTACGCAGAATAAACCCGATGCAACGAATCTTTGGGGTTTCTCTGGTTTAGAACAAGCGGTTTCTCTTTGTGAGCATCCT
ATTGTCGCTATTGGTGGTATTGACGAAAGCAACGCATCTAAAGTCATTGACGCTGGCGCTGCAGGTATAGCAGCTATAGG
AGTATTCCATAGCGCACAAAACCCCAGCTCAGTAACGAAAGCTCTACGGGAAATTGTTGATCGAGGACTGAGATGCTAG

Upstream 100 bases:

>100_bases
AGAGAGTTGCGTTCATTTTTGTTCTTAAGGGTTTTCTTTAGGGATAGTTCATAACCTCTTCTCTCTTGCGTAAACGTGTG
CATATGTAGGGGGAAAACCT

Downstream 100 bases:

>100_bases
AACGCATGCAAGAAGCGTTGCAACGCTTAAGAAAGGAAAGGCCCGTAATTTTAAACACCACGAATTATGTTTCTATGGAT
TTCCTAGCGAACTGTTTTCT

Product: putative thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MEENFFKLILITNKQQISVEEYLDFVCACVQSGVTSVQLREKELSYRELLGFGEALKSILDPLEIPLIVSDSVSVCLDLD
ATGVHLGQTDGDVIEARELLGSDKIIGWNVNTLDQLLNANTLPIDYLGLSAMFATQNKPDATNLWGFSGLEQAVSLCEHP
IVAIGGIDESNASKVIDAGAAGIAAIGVFHSAQNPSSVTKALREIVDRGLRC

Sequences:

>Translated_212_residues
MEENFFKLILITNKQQISVEEYLDFVCACVQSGVTSVQLREKELSYRELLGFGEALKSILDPLEIPLIVSDSVSVCLDLD
ATGVHLGQTDGDVIEARELLGSDKIIGWNVNTLDQLLNANTLPIDYLGLSAMFATQNKPDATNLWGFSGLEQAVSLCEHP
IVAIGGIDESNASKVIDAGAAGIAAIGVFHSAQNPSSVTKALREIVDRGLRC
>Mature_212_residues
MEENFFKLILITNKQQISVEEYLDFVCACVQSGVTSVQLREKELSYRELLGFGEALKSILDPLEIPLIVSDSVSVCLDLD
ATGVHLGQTDGDVIEARELLGSDKIIGWNVNTLDQLLNANTLPIDYLGLSAMFATQNKPDATNLWGFSGLEQAVSLCEHP
IVAIGGIDESNASKVIDAGAAGIAAIGVFHSAQNPSSVTKALREIVDRGLRC

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=227, Percent_Identity=28.6343612334802, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_CHLAB (Q5L6R5)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219627.1
- HSSP:   Q8U192
- ProteinModelPortal:   Q5L6R5
- SMR:   Q5L6R5
- GeneID:   3338215
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB198
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- ProtClustDB:   CLSK392727
- BioCyc:   CABO218497:CAB198-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22791; Mature: 22791

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENFFKLILITNKQQISVEEYLDFVCACVQSGVTSVQLREKELSYRELLGFGEALKSIL
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
DPLEIPLIVSDSVSVCLDLDATGVHLGQTDGDVIEARELLGSDKIIGWNVNTLDQLLNAN
CHHHCCEEECCCCEEEEECCCCCEECCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHCCC
TLPIDYLGLSAMFATQNKPDATNLWGFSGLEQAVSLCEHPIVAIGGIDESNASKVIDAGA
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHCCC
AGIAAIGVFHSAQNPSSVTKALREIVDRGLRC
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEENFFKLILITNKQQISVEEYLDFVCACVQSGVTSVQLREKELSYRELLGFGEALKSIL
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
DPLEIPLIVSDSVSVCLDLDATGVHLGQTDGDVIEARELLGSDKIIGWNVNTLDQLLNAN
CHHHCCEEECCCCEEEEECCCCCEECCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHCCC
TLPIDYLGLSAMFATQNKPDATNLWGFSGLEQAVSLCEHPIVAIGGIDESNASKVIDAGA
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHCCC
AGIAAIGVFHSAQNPSSVTKALREIVDRGLRC
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA