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The map label for this gene is thiM

Identifier: 62184843

GI number: 62184843

Start: 216357

End: 217145

Strand: Direct

Name: thiM

Synonym: CAB199

Alternate gene names: 62184843

Gene position: 216357-217145 (Clockwise)

Preceding gene: 62184842

Following gene: 62184852

Centisome position: 18.91

GC content: 45.12

Gene sequence:

>789_bases
ATGCTAGAACGCATGCAAGAAGCGTTGCAACGCTTAAGAAAGGAAAGGCCCGTAATTTTAAACACCACGAATTATGTTTC
TATGGATTTCCTAGCGAACTGTTTTCTAGCCATCGGAGCGTCGCCTATTATGAGCGTGTCCGATTTAGAGTTGGAAGAGT
TAATAGAGTTGAGTTCGGCCGTGTATATTAACATTGGCACTCTCGATCATTTATTTATTCAACGAGCGTACAGAACTGTA
GACCTGGCTGTAAGACAAAATAAACCTGTGATTTTTGACCCTGTGGCTGCAGGAGCTACAAAGATTAGAACAGAGGTGTC
TCATCACTTACTGGCCCATGCGACAATCGTTCGAGGAAATGCTAGTGAGATCCTTTCTTTCGGGGATGTCACGATGAAAA
CACGTGGTGTAGATTCTACTCACTCTACCCAAGACGCTAAAGATGTGGCCACCGCTCTAGCGAAAGAATGTTTGTGCGGT
TGTGCTATTGCTGTTTCCGGAGCTATAGATTTTATTACTGACGGGCAGCGTCATACAACTGTGGAGCTTGGCGATCCGTT
CATGTCTTATGTTGTGGGCATGGGATGTTCTTTAACCGGAGTATTCGCTGCGTTCAGATCGGTCATAGACGATTCTTTTG
AAGCCACGAAATTAGGTATAGAATACTTCACTCTGTGCGGCATGCTGGCTCGTGAGCGCTGTGAAGGCCCTGGATTGTTT
AAGGCTTATCTCCTTGACGAGTTGTATGCTTCCGACTTTAGTAGGATGCGTCAATACTACGATCGATAA

Upstream 100 bases:

>100_bases
CTGGCGCTGCAGGTATAGCAGCTATAGGAGTATTCCATAGCGCACAAAACCCCAGCTCAGTAACGAAAGCTCTACGGGAA
ATTGTTGATCGAGGACTGAG

Downstream 100 bases:

>100_bases
ACGCTATATCAAATAGAGAACTGAATCTTGTTGAGTTGTGTAATTGTAGAACCTAAATCTAGATTCTCAATTACACAACA
TGGTCTCTACAAAAAGCGCG

Product: putative hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLERMQEALQRLRKERPVILNTTNYVSMDFLANCFLAIGASPIMSVSDLELEELIELSSAVYINIGTLDHLFIQRAYRTV
DLAVRQNKPVIFDPVAAGATKIRTEVSHHLLAHATIVRGNASEILSFGDVTMKTRGVDSTHSTQDAKDVATALAKECLCG
CAIAVSGAIDFITDGQRHTTVELGDPFMSYVVGMGCSLTGVFAAFRSVIDDSFEATKLGIEYFTLCGMLARERCEGPGLF
KAYLLDELYASDFSRMRQYYDR

Sequences:

>Translated_262_residues
MLERMQEALQRLRKERPVILNTTNYVSMDFLANCFLAIGASPIMSVSDLELEELIELSSAVYINIGTLDHLFIQRAYRTV
DLAVRQNKPVIFDPVAAGATKIRTEVSHHLLAHATIVRGNASEILSFGDVTMKTRGVDSTHSTQDAKDVATALAKECLCG
CAIAVSGAIDFITDGQRHTTVELGDPFMSYVVGMGCSLTGVFAAFRSVIDDSFEATKLGIEYFTLCGMLARERCEGPGLF
KAYLLDELYASDFSRMRQYYDR
>Mature_262_residues
MLERMQEALQRLRKERPVILNTTNYVSMDFLANCFLAIGASPIMSVSDLELEELIELSSAVYINIGTLDHLFIQRAYRTV
DLAVRQNKPVIFDPVAAGATKIRTEVSHHLLAHATIVRGNASEILSFGDVTMKTRGVDSTHSTQDAKDVATALAKECLCG
CAIAVSGAIDFITDGQRHTTVELGDPFMSYVVGMGCSLTGVFAAFRSVIDDSFEATKLGIEYFTLCGMLARERCEGPGLF
KAYLLDELYASDFSRMRQYYDR

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=244, Percent_Identity=36.4754098360656, Blast_Score=150, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6325042, Length=273, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_CHLAB (Q5L6R4)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219628.1
- HSSP:   O58877
- ProteinModelPortal:   Q5L6R4
- SMR:   Q5L6R4
- GeneID:   3338216
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB199
- HOGENOM:   HBG351126
- OMA:   TISESTY
- PhylomeDB:   Q5L6R4
- ProtClustDB:   CLSK392728
- BioCyc:   CABO218497:CAB199-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28948; Mature: 28948

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 118-118 BINDING 166-166 BINDING 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERMQEALQRLRKERPVILNTTNYVSMDFLANCFLAIGASPIMSVSDLELEELIELSSA
CHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCE
VYINIGTLDHLFIQRAYRTVDLAVRQNKPVIFDPVAAGATKIRTEVSHHLLAHATIVRGN
EEEEECHHHHHHHHHHHHHHHHEEECCCCEEECCHHCCHHHHHHHHHHHHHHHHHHCCCC
ASEILSFGDVTMKTRGVDSTHSTQDAKDVATALAKECLCGCAIAVSGAIDFITDGQRHTT
HHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEE
VELGDPFMSYVVGMGCSLTGVFAAFRSVIDDSFEATKLGIEYFTLCGMLARERCEGPGLF
EEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
KAYLLDELYASDFSRMRQYYDR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLERMQEALQRLRKERPVILNTTNYVSMDFLANCFLAIGASPIMSVSDLELEELIELSSA
CHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCE
VYINIGTLDHLFIQRAYRTVDLAVRQNKPVIFDPVAAGATKIRTEVSHHLLAHATIVRGN
EEEEECHHHHHHHHHHHHHHHHEEECCCCEEECCHHCCHHHHHHHHHHHHHHHHHHCCCC
ASEILSFGDVTMKTRGVDSTHSTQDAKDVATALAKECLCGCAIAVSGAIDFITDGQRHTT
HHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEE
VELGDPFMSYVVGMGCSLTGVFAAFRSVIDDSFEATKLGIEYFTLCGMLARERCEGPGLF
EEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
KAYLLDELYASDFSRMRQYYDR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA