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The map label for this gene is mscS [H]

Identifier: 59714290

GI number: 59714290

Start: 1248864

End: 1250468

Strand: Reverse

Name: mscS [H]

Synonym: VF_A1107

Alternate gene names: 59714290

Gene position: 1250468-1248864 (Counterclockwise)

Preceding gene: 59714291

Following gene: 59714285

Centisome position: 94.0

GC content: 36.7

Gene sequence:

>1605_bases
ATGATTAAAAAGTATTTTGCCGCGTTGTTATTAATGATGAGTATGATGGCTCCTGCCTACGCTCAATCTATTGATAGCAT
GGTTACGCTTCAACAAGAGTTAAAAGAAAGATTAGTTCAGTTATCAACGGCTGATCCTGCCGATAAATTGTTTTTTGAAG
ATGTATTGCGTCGTAAAAATGACAAGATTCGAGCTGATATTGAACTACAACTACAAGATAATCCAAGTGTAAAGGGGTTA
GATGAGGTTGTTCGTTCAGAGATGACGCTTATGGGGAACCTATTGAAGTTAAGCCATCAAGATCTGGTTGAGTTAACTAA
AGCGGTTCGTAAAGCAAAGGGTGAAGAGAAAGACGATTTAGAATTTGCGGCGCAAAAACGTCTTGGCATTATGGATGTAT
ATTATGAAGAAGCATTCAAAACACTGTCATGGGGCGAAACTCTAAATATTGATTTCACTAAAGAAAAAACAGCGATTTTA
AAAGAGTTAACAAATCGTGCTGATTTGTTATCTAATATCATTCATTACCTTGAGTCACAAAAGTCAGATATTGCTAAGCG
TATGCAATATGCATCTGCTGAGAAGAAAGACCTTCTTACGCAAAATGCGACTAGCTTGACTGAACGTATTTCATTAATGA
TTGCGAGTTTAGAAGTAACCACCGCATTAATGGAACAAAATAACATTGATATTACAGAATATAAGCAGCTTATCTTCTCT
GTGACAGGTGATATTAACAGTGATGTATTAGATATCAGCGTTGCATATGGTCTAACTGAAGAGTGGGTTACTCGCTTTAA
AGATTGGGCGGTAGAAAATGCACCATCTATCATTGTTAAGTTAATCATTTTTGCCGTTATTCTTTGGATTACGAATTTCC
TTTCTCGTCTTGCGAAGAGTGCAATTAAGAGAAGCGTTTCTCACTCAAAAATGAAATTCAGCAAGTTAATGCAAGAGTTC
TTTGTGTCTATGGGCTCAAAGTTTGTCATGATGATTGGTATTTTAATTGCTCTATCTCAAATCGGTATTAATTTAGGTCC
ATTATTAACTGGTTTTGGTGTTGCTGGTGTTGTTATTGGTTTTGCATTAAAAGACACGTTATCTAACTTTGCATCTGGCA
TGATGATCCTGATTTATCGTCCGTTTGATGTGGGTGATTTGGTTGATATTGGTGGCATTGTGGGTAAAGTTAATCGCATG
AACCTGGTATCAACAACGATTAAAACCGTTGATAACCAAAACTTAATCTTCCCTAATAATAAGATTTGGGGTGACGTAAT
TAAGAACGTAACTATCGAAACTGAGCGTCGTGTAGACATGGTGTTTGGTATTGGTTATCAAGACGATATTGAAAAAGCAA
AAGCTATCTTTACTGATATTTTAGAAAGTCACCCAGCAGTATTAAAAACACCAGAACCTATGGTTAAATTGCATACTCTA
AATGAATCATCTGTGGACTTTATTGTGCGCCCTTGGGTTAAGACTGATGATTACTGGGATGTGTACTGGGAAGTAACTGA
AACCGTTAAAAAACGTTTGGATGCGGAAGGTGTGAGTATTCCGTTCCCACAACGTGACCTGCATATTTATAAACACGAAG
CATAA

Upstream 100 bases:

>100_bases
ACAAGCTTTCATCATTCGTCACTGTTATTTGTATTTGTTATAGATGAGAATAGCTTGGTATCAATTATTATATGGTCATG
ATTTATATAGGACGTTTTTT

Downstream 100 bases:

>100_bases
AATGCAGAAACAAAAAAGAGTGACCAAACGGTCACTCTTTTTTTATGGATTCTAATCTAGATTATCTTCTGATTTGGTTT
CTATCTGAGTCGGTTTTTTA

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MIKKYFAALLLMMSMMAPAYAQSIDSMVTLQQELKERLVQLSTADPADKLFFEDVLRRKNDKIRADIELQLQDNPSVKGL
DEVVRSEMTLMGNLLKLSHQDLVELTKAVRKAKGEEKDDLEFAAQKRLGIMDVYYEEAFKTLSWGETLNIDFTKEKTAIL
KELTNRADLLSNIIHYLESQKSDIAKRMQYASAEKKDLLTQNATSLTERISLMIASLEVTTALMEQNNIDITEYKQLIFS
VTGDINSDVLDISVAYGLTEEWVTRFKDWAVENAPSIIVKLIIFAVILWITNFLSRLAKSAIKRSVSHSKMKFSKLMQEF
FVSMGSKFVMMIGILIALSQIGINLGPLLTGFGVAGVVIGFALKDTLSNFASGMMILIYRPFDVGDLVDIGGIVGKVNRM
NLVSTTIKTVDNQNLIFPNNKIWGDVIKNVTIETERRVDMVFGIGYQDDIEKAKAIFTDILESHPAVLKTPEPMVKLHTL
NESSVDFIVRPWVKTDDYWDVYWEVTETVKKRLDAEGVSIPFPQRDLHIYKHEA

Sequences:

>Translated_534_residues
MIKKYFAALLLMMSMMAPAYAQSIDSMVTLQQELKERLVQLSTADPADKLFFEDVLRRKNDKIRADIELQLQDNPSVKGL
DEVVRSEMTLMGNLLKLSHQDLVELTKAVRKAKGEEKDDLEFAAQKRLGIMDVYYEEAFKTLSWGETLNIDFTKEKTAIL
KELTNRADLLSNIIHYLESQKSDIAKRMQYASAEKKDLLTQNATSLTERISLMIASLEVTTALMEQNNIDITEYKQLIFS
VTGDINSDVLDISVAYGLTEEWVTRFKDWAVENAPSIIVKLIIFAVILWITNFLSRLAKSAIKRSVSHSKMKFSKLMQEF
FVSMGSKFVMMIGILIALSQIGINLGPLLTGFGVAGVVIGFALKDTLSNFASGMMILIYRPFDVGDLVDIGGIVGKVNRM
NLVSTTIKTVDNQNLIFPNNKIWGDVIKNVTIETERRVDMVFGIGYQDDIEKAKAIFTDILESHPAVLKTPEPMVKLHTL
NESSVDFIVRPWVKTDDYWDVYWEVTETVKKRLDAEGVSIPFPQRDLHIYKHEA
>Mature_534_residues
MIKKYFAALLLMMSMMAPAYAQSIDSMVTLQQELKERLVQLSTADPADKLFFEDVLRRKNDKIRADIELQLQDNPSVKGL
DEVVRSEMTLMGNLLKLSHQDLVELTKAVRKAKGEEKDDLEFAAQKRLGIMDVYYEEAFKTLSWGETLNIDFTKEKTAIL
KELTNRADLLSNIIHYLESQKSDIAKRMQYASAEKKDLLTQNATSLTERISLMIASLEVTTALMEQNNIDITEYKQLIFS
VTGDINSDVLDISVAYGLTEEWVTRFKDWAVENAPSIIVKLIIFAVILWITNFLSRLAKSAIKRSVSHSKMKFSKLMQEF
FVSMGSKFVMMIGILIALSQIGINLGPLLTGFGVAGVVIGFALKDTLSNFASGMMILIYRPFDVGDLVDIGGIVGKVNRM
NLVSTTIKTVDNQNLIFPNNKIWGDVIKNVTIETERRVDMVFGIGYQDDIEKAKAIFTDILESHPAVLKTPEPMVKLHTL
NESSVDFIVRPWVKTDDYWDVYWEVTETVKKRLDAEGVSIPFPQRDLHIYKHEA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=263, Percent_Identity=32.319391634981, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1786670, Length=208, Percent_Identity=25.9615384615385, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787591, Length=212, Percent_Identity=28.7735849056604, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367355, Length=198, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 60496; Mature: 60496

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKYFAALLLMMSMMAPAYAQSIDSMVTLQQELKERLVQLSTADPADKLFFEDVLRRKN
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
DKIRADIELQLQDNPSVKGLDEVVRSEMTLMGNLLKLSHQDLVELTKAVRKAKGEEKDDL
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EFAAQKRLGIMDVYYEEAFKTLSWGETLNIDFTKEKTAILKELTNRADLLSNIIHYLESQ
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSDIAKRMQYASAEKKDLLTQNATSLTERISLMIASLEVTTALMEQNNIDITEYKQLIFS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VTGDINSDVLDISVAYGLTEEWVTRFKDWAVENAPSIIVKLIIFAVILWITNFLSRLAKS
HCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AIKRSVSHSKMKFSKLMQEFFVSMGSKFVMMIGILIALSQIGINLGPLLTGFGVAGVVIG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FALKDTLSNFASGMMILIYRPFDVGDLVDIGGIVGKVNRMNLVSTTIKTVDNQNLIFPNN
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCC
KIWGDVIKNVTIETERRVDMVFGIGYQDDIEKAKAIFTDILESHPAVLKTPEPMVKLHTL
CHHHHHHHCCEECCCCCEEHEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEC
NESSVDFIVRPWVKTDDYWDVYWEVTETVKKRLDAEGVSIPFPQRDLHIYKHEA
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MIKKYFAALLLMMSMMAPAYAQSIDSMVTLQQELKERLVQLSTADPADKLFFEDVLRRKN
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
DKIRADIELQLQDNPSVKGLDEVVRSEMTLMGNLLKLSHQDLVELTKAVRKAKGEEKDDL
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EFAAQKRLGIMDVYYEEAFKTLSWGETLNIDFTKEKTAILKELTNRADLLSNIIHYLESQ
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSDIAKRMQYASAEKKDLLTQNATSLTERISLMIASLEVTTALMEQNNIDITEYKQLIFS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VTGDINSDVLDISVAYGLTEEWVTRFKDWAVENAPSIIVKLIIFAVILWITNFLSRLAKS
HCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AIKRSVSHSKMKFSKLMQEFFVSMGSKFVMMIGILIALSQIGINLGPLLTGFGVAGVVIG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FALKDTLSNFASGMMILIYRPFDVGDLVDIGGIVGKVNRMNLVSTTIKTVDNQNLIFPNN
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCC
KIWGDVIKNVTIETERRVDMVFGIGYQDDIEKAKAIFTDILESHPAVLKTPEPMVKLHTL
CHHHHHHHCCEECCCCCEEHEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEC
NESSVDFIVRPWVKTDDYWDVYWEVTETVKKRLDAEGVSIPFPQRDLHIYKHEA
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]