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The map label for this gene is nagE2 [H]

Identifier: 59713621

GI number: 59713621

Start: 501042

End: 502499

Strand: Reverse

Name: nagE2 [H]

Synonym: VF_A0438

Alternate gene names: 59713621

Gene position: 502499-501042 (Counterclockwise)

Preceding gene: 59713622

Following gene: 59713619

Centisome position: 37.77

GC content: 40.67

Gene sequence:

>1458_bases
ATGAGTGTATTAGGTTACATGCAAAAAGTCGGTAAAGCGCTGATGGTTCCCGTCGCTACGCTTCCTGCTGCGGCAATATT
AATGGGTGTTGGTTACTGGATGGATCCGACTGGTTGGGGTGCAAACAGTGTTTTTGCTGCTTTCTTAATCAAATCAGGTG
GTGCCATTATTGATGTAATGCCAATGCTTTTTGCTATTGGTGTGGCATTTGGTTTAAGTAAAGATAAAAACGGTTCTGCA
GCATTAGCTGGTTTCATCTCTTTCACTGTGGTTACCACCCTTCTTTCTCCTGCGGTAGTGGCTCAGCTTGAAAACATTCC
TTTGGATCAAGTTCCTGCTGCATTTGGTAAAATCAATAACCAATTTGTTGGTATTATCGTTGGTATTATTTCAGCTGAAA
TTTACAACAAATACTCAACGGTTGAATTACCTAAAGCATTAGCTTTCTTCAGTGGTAAACGTCTGGTTCCGATCCTTACT
TCAATTGCAGGTATGGCTTTATCATTCGTTCTTCTGTACGTATGGCCTGCTATTTTTGATGCATTGATCGCTTTTGGTAC
ACAACTTCAATCAATGGGAGCTGTTGGCGCTGGCCTATTTGGTTTCTTTAACCGCTTAATGCTAACTGTTGGTATGCACC
ATGCACTTTACCCTGTATTCTGGTTTGATGTTGTTGGTATTAACGATATTCCAAACTTCTTAGGTGGCGCACAGTCTATT
GCTAATGGTACTGGTATTCCAGGCCAAACAGGTATGTACCAAGCTGGTTTCTTCCCTATAATGATGTTTGGTCTTCCAGG
CGCTGCACTTGCTATTTACCACTGTGCTGAAAAACGTAACAAAGCTGCGGTATTCTCTATCATGCTTGCAGCTGCAATGG
CTTCATTCTTTACAGGTATCACTGAACCTCTAGAGTTTAGCTTCATGTTCCTTGCTCCATGGTTATACCTAATTCATGCA
ATCTTAACAGGCCTATCTCTATTCATTGCAGCAAGCATGGAATGGATGGCAGGTTTTGGTTTCTCTGCAGGTCTTGTTGA
CTTTGTTCTTTCAAGTCAAAACCCACTAGCTGTAAAATGGTACATGTTGATCCCACAAGGTCTTGTATTCTTTGTTCTTT
ACTACACTATCTTTAGATTTGCGATTAAGAAATTCAACTTCTTAACTCCTGGCCGTGGCGAAGACATGAATAAAGATGAG
CAAGAAGAAAACATCACAGAAGTAACAAAAGCGTATATCGAAGCGATTGGTGGTGCAGATAATATTGTTGAAGTAGATAA
CTGTATTACTCGTTTACGTTTAACGGTAAAAGACTCAGCCCTTGCTGACAGTGCAAAATTAAAAGCTCTTGGTGCAGCAG
GCGTTGTTCCAATGGGTAAAGGTGGTTTACAAGTCATTATTGGCTTAGGAAAAGTAGACAAAGTTGCAGCAGAAATGAAA
GCAATTTTGAACGCTTAA

Upstream 100 bases:

>100_bases
CATTAACTATAGTTAATGTTGGCACAATCTTTTGTTAATCCACCCTTTTGTATTCTTCTCTTAGTTTAAAAAATAACAGA
TTAATTTTGAGGAATACGAT

Downstream 100 bases:

>100_bases
CTTCATAATATTCGTACCAGATATTATGTTGTAAACTCAGAGCCTTAATGTTCCCCCGTTAAGGCTCTTTATCTTTACAA
TGCAACCTGATGTTTCCGCC

Product: fused N-acetyl glucosamine specific PTS enzyme: IIC and IIB components

Products: NA

Alternate protein names: EIICBA-Glc; EII-Glc; Glucose permease IIC component; PTS system glucose-specific EIIC component; Glucose-specific phosphotransferase enzyme IIB component; PTS system glucose-specific EIIB component; Glucose-specific phosphotransferase enzyme IIA component; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSVLGYMQKVGKALMVPVATLPAAAILMGVGYWMDPTGWGANSVFAAFLIKSGGAIIDVMPMLFAIGVAFGLSKDKNGSA
ALAGFISFTVVTTLLSPAVVAQLENIPLDQVPAAFGKINNQFVGIIVGIISAEIYNKYSTVELPKALAFFSGKRLVPILT
SIAGMALSFVLLYVWPAIFDALIAFGTQLQSMGAVGAGLFGFFNRLMLTVGMHHALYPVFWFDVVGINDIPNFLGGAQSI
ANGTGIPGQTGMYQAGFFPIMMFGLPGAALAIYHCAEKRNKAAVFSIMLAAAMASFFTGITEPLEFSFMFLAPWLYLIHA
ILTGLSLFIAASMEWMAGFGFSAGLVDFVLSSQNPLAVKWYMLIPQGLVFFVLYYTIFRFAIKKFNFLTPGRGEDMNKDE
QEENITEVTKAYIEAIGGADNIVEVDNCITRLRLTVKDSALADSAKLKALGAAGVVPMGKGGLQVIIGLGKVDKVAAEMK
AILNA

Sequences:

>Translated_485_residues
MSVLGYMQKVGKALMVPVATLPAAAILMGVGYWMDPTGWGANSVFAAFLIKSGGAIIDVMPMLFAIGVAFGLSKDKNGSA
ALAGFISFTVVTTLLSPAVVAQLENIPLDQVPAAFGKINNQFVGIIVGIISAEIYNKYSTVELPKALAFFSGKRLVPILT
SIAGMALSFVLLYVWPAIFDALIAFGTQLQSMGAVGAGLFGFFNRLMLTVGMHHALYPVFWFDVVGINDIPNFLGGAQSI
ANGTGIPGQTGMYQAGFFPIMMFGLPGAALAIYHCAEKRNKAAVFSIMLAAAMASFFTGITEPLEFSFMFLAPWLYLIHA
ILTGLSLFIAASMEWMAGFGFSAGLVDFVLSSQNPLAVKWYMLIPQGLVFFVLYYTIFRFAIKKFNFLTPGRGEDMNKDE
QEENITEVTKAYIEAIGGADNIVEVDNCITRLRLTVKDSALADSAKLKALGAAGVVPMGKGGLQVIIGLGKVDKVAAEMK
AILNA
>Mature_484_residues
SVLGYMQKVGKALMVPVATLPAAAILMGVGYWMDPTGWGANSVFAAFLIKSGGAIIDVMPMLFAIGVAFGLSKDKNGSAA
LAGFISFTVVTTLLSPAVVAQLENIPLDQVPAAFGKINNQFVGIIVGIISAEIYNKYSTVELPKALAFFSGKRLVPILTS
IAGMALSFVLLYVWPAIFDALIAFGTQLQSMGAVGAGLFGFFNRLMLTVGMHHALYPVFWFDVVGINDIPNFLGGAQSIA
NGTGIPGQTGMYQAGFFPIMMFGLPGAALAIYHCAEKRNKAAVFSIMLAAAMASFFTGITEPLEFSFMFLAPWLYLIHAI
LTGLSLFIAASMEWMAGFGFSAGLVDFVLSSQNPLAVKWYMLIPQGLVFFVLYYTIFRFAIKKFNFLTPGRGEDMNKDEQ
EENITEVTKAYIEAIGGADNIVEVDNCITRLRLTVKDSALADSAKLKALGAAGVVPMGKGGLQVIIGLGKVDKVAAEMKA
ILNA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=500, Percent_Identity=41, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1787343, Length=489, Percent_Identity=38.6503067484663, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1787908, Length=519, Percent_Identity=34.4894026974952, Blast_Score=261, Evalue=9e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011299 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 51765; Mature: 51634

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCC
>Mature Secondary Structure 
SVLGYMQKVGKALMVPVATLPAAAILMGVGYWMDPTGWGANSVFAAFLIKSGGAIIDVM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHH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HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA