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The map label for this gene is nnrS

Identifier: 59713622

GI number: 59713622

Start: 502654

End: 503838

Strand: Reverse

Name: nnrS

Synonym: VF_A0439

Alternate gene names: NA

Gene position: 503838-502654 (Counterclockwise)

Preceding gene: 59713624

Following gene: 59713621

Centisome position: 37.87

GC content: 40.34

Gene sequence:

>1185_bases
ATGATAAATATTACTGATAAAGCCATTGAAGAAAAGATCCCACCGATCCTACGCTTAGGCTTTCGTCCTTTCTTCTTACT
AGGCAGCATTTATGCAATTATCGCCGTCGTAATTTGGGTTTGGGCTTTCCAAACGGGTCAACCGGCTTACCTACAAGTTC
CTGCACTGTGGTGGCATGTTCACGAAATGTTGTTTGGTTTTTCTATGGCTATTGTTGCCGGCTTTGTTTTAAGTGCGGTT
CAAAACTGGACTGGGGTTAAAGGTACATCCAATAAACGTCTCGGGTTCATTGTCTTATTATGGTTATTACCAAGAGTTTT
ATTTTGGACTCCATCTCCACTATGGCTAACATCAAGCATAGAGGCCCTATTTATACTTGCTATTGCCTATGAAGTAGGTT
TCCGTGTAATTAAAACAAAAGGTGTGAGAAACTTCTTTTTCATTCCATTATTTTTAGTGGCTATCGCAGCCAACTTTGCT
AGTTATGCAACGATCAAAGGAATGCCCCCTTTTACTTCTGCTGCAGTTTGGGAGTCTATGCTTTGGTGGTTTACTTTATT
ACTTTCCGTAATGGGTGCTCGTGTCATTCCTTTTTTTACCGCTCGTCGCTTTCAGTTTGAAAAGGCACAACCAATTCTTT
GGTTAGATGCGTTATGTAATATCCCATTAGCTATGCTCTTTATTTTGAGCTTTTTCCCTCTAACGTCAGAACAAGTAAGT
CCATATCTGATGATTATTGCAGGCGTTGCTCAATTAATCAGAATGATCCGTTGGAAAGGATATAAAACACTGAGTGAACC
ACTAGTTTGGTCACTTCATGCTGGCTATTTATGTATCCCACTAAGCTTATTGTTACGTGGTTTCAGTACCGATTATTTTA
TCTCTCATACAGGAATTCATTTATTTGCTATTGGTGCCTTAGGCGGCGTTATTTTAGCTATGATTGCTCGAGTAACTATG
GGACATACTGGCCGTGAGATCTATAAAGGCCCGTCAATGTGGGTTGCTTATAGCGCCATCATCTTAGCGGCATTTATCCG
TTCATTTGGAGTAATTGCATTTCCTGAATATATGCTCGATATGATCAACATTGCTGCAATTCTCTGGACTATCGCATTTG
GGCTATTCATTTGGCATTTTGCGCCTATGCTATGGTCAAAACGCGTAGATGGACATCCAGGTTAA

Upstream 100 bases:

>100_bases
AAAAAAAGAGAATGAACCTCTTAAACATGCATAAAAAATACATGTTATTATTTTCCCTTTGCGTTATACTTGTTTTGTCA
CTAAAAATTGAGAACAAATT

Downstream 100 bases:

>100_bases
TTTACGCCTCGAATTAATTAGATCTCACTCACATTTTAATTCTTAAAGTGCCAACATTAACTATAGTTAATGTTGGCACA
ATCTTTTGTTAATCCACCCT

Product: nitrite and/or nitric oxide reductase

Products: AMP; diphosphate; L-asparagine; L-glutamate

Alternate protein names: NnrS Protein; Heme/Copper Membrane Protein; Protein Involved In Response NO; Heme-Cu Protein NnrS; Nitrite/Nitric Oxide Reductase-Related Protein NnrS; NnrS-Like Protein; Asparagine Synthetase B; ATP Synthase F0 Subunit A; NnrS Transmembrane Protein; Heme-Cu Membrane Protein; Transmembrane Protein; Heme-Cu-Containing Membrane Protein; LOW QUALITY PROTEIN NnrS Family Protein; Nnrs Family Protein; Heme/Copper Membrane Protein Of NnrS Family Protein; ATP Synthase F0 A Subunit; Nitrite And/Or Nitric Oxide Reductase; Integral Inner Membrane Permease; NnrS Protein Superfamily; Asparagine Synthetase

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MINITDKAIEEKIPPILRLGFRPFFLLGSIYAIIAVVIWVWAFQTGQPAYLQVPALWWHVHEMLFGFSMAIVAGFVLSAV
QNWTGVKGTSNKRLGFIVLLWLLPRVLFWTPSPLWLTSSIEALFILAIAYEVGFRVIKTKGVRNFFFIPLFLVAIAANFA
SYATIKGMPPFTSAAVWESMLWWFTLLLSVMGARVIPFFTARRFQFEKAQPILWLDALCNIPLAMLFILSFFPLTSEQVS
PYLMIIAGVAQLIRMIRWKGYKTLSEPLVWSLHAGYLCIPLSLLLRGFSTDYFISHTGIHLFAIGALGGVILAMIARVTM
GHTGREIYKGPSMWVAYSAIILAAFIRSFGVIAFPEYMLDMINIAAILWTIAFGLFIWHFAPMLWSKRVDGHPG

Sequences:

>Translated_394_residues
MINITDKAIEEKIPPILRLGFRPFFLLGSIYAIIAVVIWVWAFQTGQPAYLQVPALWWHVHEMLFGFSMAIVAGFVLSAV
QNWTGVKGTSNKRLGFIVLLWLLPRVLFWTPSPLWLTSSIEALFILAIAYEVGFRVIKTKGVRNFFFIPLFLVAIAANFA
SYATIKGMPPFTSAAVWESMLWWFTLLLSVMGARVIPFFTARRFQFEKAQPILWLDALCNIPLAMLFILSFFPLTSEQVS
PYLMIIAGVAQLIRMIRWKGYKTLSEPLVWSLHAGYLCIPLSLLLRGFSTDYFISHTGIHLFAIGALGGVILAMIARVTM
GHTGREIYKGPSMWVAYSAIILAAFIRSFGVIAFPEYMLDMINIAAILWTIAFGLFIWHFAPMLWSKRVDGHPG
>Mature_394_residues
MINITDKAIEEKIPPILRLGFRPFFLLGSIYAIIAVVIWVWAFQTGQPAYLQVPALWWHVHEMLFGFSMAIVAGFVLSAV
QNWTGVKGTSNKRLGFIVLLWLLPRVLFWTPSPLWLTSSIEALFILAIAYEVGFRVIKTKGVRNFFFIPLFLVAIAANFA
SYATIKGMPPFTSAAVWESMLWWFTLLLSVMGARVIPFFTARRFQFEKAQPILWLDALCNIPLAMLFILSFFPLTSEQVS
PYLMIIAGVAQLIRMIRWKGYKTLSEPLVWSLHAGYLCIPLSLLLRGFSTDYFISHTGIHLFAIGALGGVILAMIARVTM
GHTGREIYKGPSMWVAYSAIILAAFIRSFGVIAFPEYMLDMINIAAILWTIAFGLFIWHFAPMLWSKRVDGHPG

Specific function: Unknown

COG id: COG3213

COG function: function code P; Uncharacterized protein involved in response to NO

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.5.4

Molecular weight: Translated: 44515; Mature: 44515

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINITDKAIEEKIPPILRLGFRPFFLLGSIYAIIAVVIWVWAFQTGQPAYLQVPALWWHV
CCCCCHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
HEMLFGFSMAIVAGFVLSAVQNWTGVKGTSNKRLGFIVLLWLLPRVLFWTPSPLWLTSSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
EALFILAIAYEVGFRVIKTKGVRNFFFIPLFLVAIAANFASYATIKGMPPFTSAAVWESM
HHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHH
LWWFTLLLSVMGARVIPFFTARRFQFEKAQPILWLDALCNIPLAMLFILSFFPLTSEQVS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHCC
PYLMIIAGVAQLIRMIRWKGYKTLSEPLVWSLHAGYLCIPLSLLLRGFSTDYFISHTGIH
HHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCHH
LFAIGALGGVILAMIARVTMGHTGREIYKGPSMWVAYSAIILAAFIRSFGVIAFPEYMLD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
MINIAAILWTIAFGLFIWHFAPMLWSKRVDGHPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MINITDKAIEEKIPPILRLGFRPFFLLGSIYAIIAVVIWVWAFQTGQPAYLQVPALWWHV
CCCCCHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
HEMLFGFSMAIVAGFVLSAVQNWTGVKGTSNKRLGFIVLLWLLPRVLFWTPSPLWLTSSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
EALFILAIAYEVGFRVIKTKGVRNFFFIPLFLVAIAANFASYATIKGMPPFTSAAVWESM
HHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHH
LWWFTLLLSVMGARVIPFFTARRFQFEKAQPILWLDALCNIPLAMLFILSFFPLTSEQVS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHCC
PYLMIIAGVAQLIRMIRWKGYKTLSEPLVWSLHAGYLCIPLSLLLRGFSTDYFISHTGIH
HHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCHH
LFAIGALGGVILAMIARVTMGHTGREIYKGPSMWVAYSAIILAAFIRSFGVIAFPEYMLD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
MINIAAILWTIAFGLFIWHFAPMLWSKRVDGHPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-aspartate; L-glutamine

Specific reaction: ATP + L-aspartate + L-glutamine = AMP + diphosphate + L-asparagine + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA