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The map label for this gene is gspL [H]

Identifier: 59713073

GI number: 59713073

Start: 2763090

End: 2764343

Strand: Reverse

Name: gspL [H]

Synonym: VF_2466

Alternate gene names: 59713073

Gene position: 2764343-2763090 (Counterclockwise)

Preceding gene: 59713074

Following gene: 59713072

Centisome position: 95.4

GC content: 40.75

Gene sequence:

>1254_bases
GTGAGCGAACATCTGATAATACGGTTGAATAGTCAGCCATCAGATCCCATTCAATGGATAGTCTGGTCTCCAGAAAATAA
AGAAGTGATCGCTTCGGGTGAATTAGATTCATCTGAAGCATTACCTTCGTTGTCGTCATACAGTGAACAACGCATGGTAT
CAGTGTTGCTGCCAAGCAGTGATGTGTTATTGCGCGAAGTGGTGATACCTGAAGGTGCTGCTCGTCAGTTTTCATCTATG
CTACCGTTTATTATTGAAGATGATTTAGCACAAGATGTTGATGATCTTCATATGGTTATTTTGAAAAAAGACAGCAAAGT
AGCTCAAGTGGCGATTATTGAACACAGCAAGATGACCATGTGGTTAGAACAGCTGAGCGATGCAGGGATCCAAACCAAAC
GATTCATGCCTGACGTATTGGCTTTACCATTTCATGATGATGGTGCGAGTATGGTTCAATTGGGTCAACAATGGCTTATT
CGTCATCATGAATACCAAGGTGCGGTAGCTGAGCCTCAGTGGGTATCCATGTTATTGACGGGTATCGTTGAGCGTGCTGA
AACCAAATATGAAGATTCAGAAAGTGATGAAGACGCTGAATCATCACCAATGCCTTTTATTCTTCATAGCTATTCTGAAT
GTGAAATGGAAATCGCAGGGGCTAAGGTTAACCTTGAATCACCTGAACTTGTGATGCAATTATTAGCTGAAGGATTGTTG
TCTTCAAAAGTAAACGTGCTAAATGGTCCATATCGTCCGCAATCTTCATGGAGAAAGCATTGGCGAGTATGGCAAAAAGT
GATTCTGTCTCTTGGTCTTGTGATGGTTGCTTTTGTTGCACAGCATGTTTCTGAAGTACAAAAATTAGAGCAACACAGTT
TGGCTTTGCATGCGGAAAGCGAGCGTATCTTTCGAGATATTTTCCCTAATAAGCGAAAAATACCAACGGCGAGCTATTTA
AAGTCTCAAATGAAAAATGAAGAAGCTCGTTTACAAGGTGGTGCAGGCGGACATGATTTGTTAGCTTGGATGGCAGAGAT
ATCACCTTATTTATCAAAGGTTCCTCAAGTTAAATTACAAAGCCTAAAATTTGATGGCAAGCGTAATGAAATTCGACTTC
AAGCCAGTGCTTCTGATTTCCCATATTTTGAGAAGTTGACTTCAGAGCTAGGAACTAAATTTGAAGTAGAGCAAGGTCAA
TTGAATAAGAATGACGGCCAAGTCTTTGGTGCCATTATCATTAGGAGACAATAA

Upstream 100 bases:

>100_bases
CAAGTATTAGTAGAAGATTCGCGCGTACGTGTGCGTTCTTTATTACATAGCAGTGATAAGAAAGTGGTGAACGTTATCCG
TCGCCAATATGGAGGAATGA

Downstream 100 bases:

>100_bases
TGAAAGCATTTATGGCATGGTGGCAAAGTATCTCTCCACGTGAACGTATATTGGTTGCTGGCGGTGGTGTGGCACTGTTG
ATCGCGGTTATTTACTGGGG

Product: general secretion pathway protein L

Products: NA

Alternate protein names: Cholera toxin secretion protein epsL [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSEHLIIRLNSQPSDPIQWIVWSPENKEVIASGELDSSEALPSLSSYSEQRMVSVLLPSSDVLLREVVIPEGAARQFSSM
LPFIIEDDLAQDVDDLHMVILKKDSKVAQVAIIEHSKMTMWLEQLSDAGIQTKRFMPDVLALPFHDDGASMVQLGQQWLI
RHHEYQGAVAEPQWVSMLLTGIVERAETKYEDSESDEDAESSPMPFILHSYSECEMEIAGAKVNLESPELVMQLLAEGLL
SSKVNVLNGPYRPQSSWRKHWRVWQKVILSLGLVMVAFVAQHVSEVQKLEQHSLALHAESERIFRDIFPNKRKIPTASYL
KSQMKNEEARLQGGAGGHDLLAWMAEISPYLSKVPQVKLQSLKFDGKRNEIRLQASASDFPYFEKLTSELGTKFEVEQGQ
LNKNDGQVFGAIIIRRQ

Sequences:

>Translated_417_residues
MSEHLIIRLNSQPSDPIQWIVWSPENKEVIASGELDSSEALPSLSSYSEQRMVSVLLPSSDVLLREVVIPEGAARQFSSM
LPFIIEDDLAQDVDDLHMVILKKDSKVAQVAIIEHSKMTMWLEQLSDAGIQTKRFMPDVLALPFHDDGASMVQLGQQWLI
RHHEYQGAVAEPQWVSMLLTGIVERAETKYEDSESDEDAESSPMPFILHSYSECEMEIAGAKVNLESPELVMQLLAEGLL
SSKVNVLNGPYRPQSSWRKHWRVWQKVILSLGLVMVAFVAQHVSEVQKLEQHSLALHAESERIFRDIFPNKRKIPTASYL
KSQMKNEEARLQGGAGGHDLLAWMAEISPYLSKVPQVKLQSLKFDGKRNEIRLQASASDFPYFEKLTSELGTKFEVEQGQ
LNKNDGQVFGAIIIRRQ
>Mature_416_residues
SEHLIIRLNSQPSDPIQWIVWSPENKEVIASGELDSSEALPSLSSYSEQRMVSVLLPSSDVLLREVVIPEGAARQFSSML
PFIIEDDLAQDVDDLHMVILKKDSKVAQVAIIEHSKMTMWLEQLSDAGIQTKRFMPDVLALPFHDDGASMVQLGQQWLIR
HHEYQGAVAEPQWVSMLLTGIVERAETKYEDSESDEDAESSPMPFILHSYSECEMEIAGAKVNLESPELVMQLLAEGLLS
SKVNVLNGPYRPQSSWRKHWRVWQKVILSLGLVMVAFVAQHVSEVQKLEQHSLALHAESERIFRDIFPNKRKIPTASYLK
SQMKNEEARLQGGAGGHDLLAWMAEISPYLSKVPQVKLQSLKFDGKRNEIRLQASASDFPYFEKLTSELGTKFEVEQGQL
NKNDGQVFGAIIIRRQ

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3297

COG function: function code U; Type II secretory pathway, component PulL

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP L family [H]

Homologues:

Organism=Escherichia coli, GI87082244, Length=189, Percent_Identity=24.3386243386243, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007812 [H]

Pfam domain/function: PF05134 GspL [H]

EC number: NA

Molecular weight: Translated: 47103; Mature: 46972

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHLIIRLNSQPSDPIQWIVWSPENKEVIASGELDSSEALPSLSSYSEQRMVSVLLPSS
CCCCEEEEECCCCCCCEEEEEECCCCCEEEECCCCCCHHCCCCHHHHHHHHEEEEECCCC
DVLLREVVIPEGAARQFSSMLPFIIEDDLAQDVDDLHMVILKKDSKVAQVAIIEHSKMTM
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCHHHHEEEEEECCCCEEEEEEEECCHHHH
WLEQLSDAGIQTKRFMPDVLALPFHDDGASMVQLGQQWLIRHHEYQGAVAEPQWVSMLLT
HHHHHHCCCCCHHHCCCHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
GIVERAETKYEDSESDEDAESSPMPFILHSYSECEMEIAGAKVNLESPELVMQLLAEGLL
HHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCEECCCCHHHHHHHHHHHHH
SSKVNVLNGPYRPQSSWRKHWRVWQKVILSLGLVMVAFVAQHVSEVQKLEQHSLALHAES
HCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHH
ERIFRDIFPNKRKIPTASYLKSQMKNEEARLQGGAGGHDLLAWMAEISPYLSKVPQVKLQ
HHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
SLKFDGKRNEIRLQASASDFPYFEKLTSELGTKFEVEQGQLNKNDGQVFGAIIIRRQ
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
SEHLIIRLNSQPSDPIQWIVWSPENKEVIASGELDSSEALPSLSSYSEQRMVSVLLPSS
CCCEEEEECCCCCCCEEEEEECCCCCEEEECCCCCCHHCCCCHHHHHHHHEEEEECCCC
DVLLREVVIPEGAARQFSSMLPFIIEDDLAQDVDDLHMVILKKDSKVAQVAIIEHSKMTM
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCHHHHEEEEEECCCCEEEEEEEECCHHHH
WLEQLSDAGIQTKRFMPDVLALPFHDDGASMVQLGQQWLIRHHEYQGAVAEPQWVSMLLT
HHHHHHCCCCCHHHCCCHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
GIVERAETKYEDSESDEDAESSPMPFILHSYSECEMEIAGAKVNLESPELVMQLLAEGLL
HHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCEECCCCHHHHHHHHHHHHH
SSKVNVLNGPYRPQSSWRKHWRVWQKVILSLGLVMVAFVAQHVSEVQKLEQHSLALHAES
HCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHH
ERIFRDIFPNKRKIPTASYLKSQMKNEEARLQGGAGGHDLLAWMAEISPYLSKVPQVKLQ
HHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
SLKFDGKRNEIRLQASASDFPYFEKLTSELGTKFEVEQGQLNKNDGQVFGAIIIRRQ
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]