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The map label for this gene is gspM [H]

Identifier: 59713072

GI number: 59713072

Start: 2762599

End: 2763090

Strand: Reverse

Name: gspM [H]

Synonym: VF_2465

Alternate gene names: 59713072

Gene position: 2763090-2762599 (Counterclockwise)

Preceding gene: 59713073

Following gene: 59713071

Centisome position: 95.36

GC content: 39.84

Gene sequence:

>492_bases
ATGAAAGCATTTATGGCATGGTGGCAAAGTATCTCTCCACGTGAACGTATATTGGTTGCTGGCGGTGGTGTGGCACTGTT
GATCGCGGTTATTTACTGGGGCGGAATAAAACCATTAAATGACCGTGCAGAACTTGCTCAAAATCGAATTAATAATGAAC
GTAACTTATTAGCGTGGGTTCAGAAAAAAGCCGATACGATCATTACATTAAGAGGTGGGGCATCTTCTAGCTCACAATCA
GCAATTCGTCCAATGAACCAGGTGATACCAAGTAGTACTCGTCAATTTAAAATTGAGTTAATTCGTATGCAGCCTCGTGG
TGAACAAATGCAAGTGTGGGTAAAACCATTACCTTTTAATTCTTTGGTTAATTGGCTAGCATTTTTGCGTGATTCTCAGG
GCATTAATGTTCAATTTTTAGATTTAAATAAAACTGATGTGGAAGGTGTGGTTGAAGTGAACCGTCTTCAATTAACTCGA
GAGGCATCATGA

Upstream 100 bases:

>100_bases
AGTTGACTTCAGAGCTAGGAACTAAATTTGAAGTAGAGCAAGGTCAATTGAATAAGAATGACGGCCAAGTCTTTGGTGCC
ATTATCATTAGGAGACAATA

Downstream 100 bases:

>100_bases
AGTTTAAGTTATTAATCGCGACCGTATTTTCTACTTTTTTTACCTTTAGTGCATTATTGCACATCCCAATTCAGTGGGTT
GTGGATCAAGCGCCTAAAGT

Product: cholera toxin secretion protein EpsM

Products: NA

Alternate protein names: Cholera toxin secretion protein epsM [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MKAFMAWWQSISPRERILVAGGGVALLIAVIYWGGIKPLNDRAELAQNRINNERNLLAWVQKKADTIITLRGGASSSSQS
AIRPMNQVIPSSTRQFKIELIRMQPRGEQMQVWVKPLPFNSLVNWLAFLRDSQGINVQFLDLNKTDVEGVVEVNRLQLTR
EAS

Sequences:

>Translated_163_residues
MKAFMAWWQSISPRERILVAGGGVALLIAVIYWGGIKPLNDRAELAQNRINNERNLLAWVQKKADTIITLRGGASSSSQS
AIRPMNQVIPSSTRQFKIELIRMQPRGEQMQVWVKPLPFNSLVNWLAFLRDSQGINVQFLDLNKTDVEGVVEVNRLQLTR
EAS
>Mature_163_residues
MKAFMAWWQSISPRERILVAGGGVALLIAVIYWGGIKPLNDRAELAQNRINNERNLLAWVQKKADTIITLRGGASSSSQS
AIRPMNQVIPSSTRQFKIELIRMQPRGEQMQVWVKPLPFNSLVNWLAFLRDSQGINVQFLDLNKTDVEGVVEVNRLQLTR
EAS

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3149

COG function: function code U; Type II secretory pathway, component PulM

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP M family [H]

Homologues:

Organism=Escherichia coli, GI1789339, Length=150, Percent_Identity=29.3333333333333, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007690
- InterPro:   IPR023229 [H]

Pfam domain/function: PF04612 GspM [H]

EC number: NA

Molecular weight: Translated: 18428; Mature: 18428

Theoretical pI: Translated: 11.09; Mature: 11.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFMAWWQSISPRERILVAGGGVALLIAVIYWGGIKPLNDRAELAQNRINNERNLLAWV
CCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHH
QKKADTIITLRGGASSSSQSAIRPMNQVIPSSTRQFKIELIRMQPRGEQMQVWVKPLPFN
HHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEEECCCHH
SLVNWLAFLRDSQGINVQFLDLNKTDVEGVVEVNRLQLTREAS
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHEEHHHEEEEECCC
>Mature Secondary Structure
MKAFMAWWQSISPRERILVAGGGVALLIAVIYWGGIKPLNDRAELAQNRINNERNLLAWV
CCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHH
QKKADTIITLRGGASSSSQSAIRPMNQVIPSSTRQFKIELIRMQPRGEQMQVWVKPLPFN
HHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEEECCCHH
SLVNWLAFLRDSQGINVQFLDLNKTDVEGVVEVNRLQLTREAS
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHEEHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8406031; 10952301 [H]