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The map label for this gene is ubiC

Identifier: 59713052

GI number: 59713052

Start: 2747025

End: 2747567

Strand: Reverse

Name: ubiC

Synonym: VF_2445

Alternate gene names: 59713052

Gene position: 2747567-2747025 (Counterclockwise)

Preceding gene: 59713054

Following gene: 59713051

Centisome position: 94.82

GC content: 38.86

Gene sequence:

>543_bases
ATGTCAGATATCAATTCTTGGTGTGCTTCTGTACTAAAAAGAGTGCAGTGGCAAGAGGCCGATACATTTGAGTGTAGTAA
TGACCTGCAGCGTTATTGGTTGCTAGGATTAGATTCACTATCACGTCGTTTAGAGCAGCATTGCAATGTGTTATCGGTAT
CGGTATTGGATAATACCCATGTGAATCCAGATAAATTAACCGTAGAAGAGGAAGCTCTTCTTAGCGGAGAAGTGTGTTTG
CGTCGAAAAGTCATCTTAAAAGGTGATCAGCAATCGTGGGTTTATGGTAGAACATTAATTCCGTTATCATCATTGCAAGA
TCAGCCGCATGATTTAACTCGTCAAGGTCATACACCTTTAGGTATTACTGTATTTAGTGCTCAAAGTGCACATAGAGATA
AATTACAAGTAGGAACAATAATGACAGAAAGAGGAGAGTTGTTTGCTCGTCGTTCTCGATTATGGATGAATAATAAACCT
ATGCTAGTTGCAGAGTTATTTTTACCAGAAGCACCCATTTATTCAAAGGAAGTGGAATCATGA

Upstream 100 bases:

>100_bases
AATTTATTTTATTACTTGATACAATAGTCGGCAGTTGTGAGAAAAAATTAACTACAATCTTTCAATATAAGCAATATATG
TGAAAGATGTTATAGAGAAT

Downstream 100 bases:

>100_bases
ACATGTCAAAAGCAGAGGCGTTTTGGCAACTGACCCGAATGAATCGTCCAATTGGTTCTCTATTACTTTTATGGCCAACA
TTGTGGGCTTTATTTTTAGC

Product: chorismate--pyruvate lyase

Products: NA

Alternate protein names: CL; CPL

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MSDINSWCASVLKRVQWQEADTFECSNDLQRYWLLGLDSLSRRLEQHCNVLSVSVLDNTHVNPDKLTVEEEALLSGEVCL
RRKVILKGDQQSWVYGRTLIPLSSLQDQPHDLTRQGHTPLGITVFSAQSAHRDKLQVGTIMTERGELFARRSRLWMNNKP
MLVAELFLPEAPIYSKEVES

Sequences:

>Translated_180_residues
MSDINSWCASVLKRVQWQEADTFECSNDLQRYWLLGLDSLSRRLEQHCNVLSVSVLDNTHVNPDKLTVEEEALLSGEVCL
RRKVILKGDQQSWVYGRTLIPLSSLQDQPHDLTRQGHTPLGITVFSAQSAHRDKLQVGTIMTERGELFARRSRLWMNNKP
MLVAELFLPEAPIYSKEVES
>Mature_179_residues
SDINSWCASVLKRVQWQEADTFECSNDLQRYWLLGLDSLSRRLEQHCNVLSVSVLDNTHVNPDKLTVEEEALLSGEVCLR
RKVILKGDQQSWVYGRTLIPLSSLQDQPHDLTRQGHTPLGITVFSAQSAHRDKLQVGTIMTERGELFARRSRLWMNNKPM
LVAELFLPEAPIYSKEVES

Specific function: Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4- hydroxybenzoate (4HB) for the ubiquinone pathway

COG id: COG3161

COG function: function code H; 4-hydroxybenzoate synthetase (chorismate lyase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiC family

Homologues:

Organism=Escherichia coli, GI87082361, Length=142, Percent_Identity=34.5070422535211, Blast_Score=91, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIC_VIBF1 (Q5E206)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205828.1
- ProteinModelPortal:   Q5E206
- SMR:   Q5E206
- STRING:   Q5E206
- GeneID:   3277750
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2445
- NMPDR:   fig|312309.3.peg.2445
- eggNOG:   COG3161
- HOGENOM:   HBG644467
- OMA:   ERIFWRE
- PhylomeDB:   Q5E206
- ProtClustDB:   CLSK873858
- BioCyc:   VFIS312309:VF2445-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01632
- InterPro:   IPR007440

Pfam domain/function: PF04345 Chor_lyase

EC number: =4.1.3.40

Molecular weight: Translated: 20607; Mature: 20476

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: BINDING 82-82 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDINSWCASVLKRVQWQEADTFECSNDLQRYWLLGLDSLSRRLEQHCNVLSVSVLDNTH
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEEEECCCC
VNPDKLTVEEEALLSGEVCLRRKVILKGDQQSWVYGRTLIPLSSLQDQPHDLTRQGHTPL
CCCCCEEEHHHHHHHHHHHHHHHHEEECCCCCEEECEEECCHHHCCCCCCHHHHCCCCCC
GITVFSAQSAHRDKLQVGTIMTERGELFARRSRLWMNNKPMLVAELFLPEAPIYSKEVES
EEEEEECCCCCCCHHHHHHEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SDINSWCASVLKRVQWQEADTFECSNDLQRYWLLGLDSLSRRLEQHCNVLSVSVLDNTH
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEEEECCCC
VNPDKLTVEEEALLSGEVCLRRKVILKGDQQSWVYGRTLIPLSSLQDQPHDLTRQGHTPL
CCCCCEEEHHHHHHHHHHHHHHHHEEECCCCCEEECEEECCHHHCCCCCCHHHHCCCCCC
GITVFSAQSAHRDKLQVGTIMTERGELFARRSRLWMNNKPMLVAELFLPEAPIYSKEVES
EEEEEECCCCCCCHHHHHHEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA