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The map label for this gene is purD [H]

Identifier: 59713002

GI number: 59713002

Start: 2682849

End: 2684141

Strand: Direct

Name: purD [H]

Synonym: VF_2395

Alternate gene names: 59713002

Gene position: 2682849-2684141 (Clockwise)

Preceding gene: 59713001

Following gene: 59713005

Centisome position: 92.59

GC content: 44.93

Gene sequence:

>1293_bases
ATGAATGTATTAATTATTGGTGCTGGCGGTCGTGAGCACGCACTAGGTTGGAAAGCAGCACAAAACCCAAATGTTGAAAC
AGTATTCATCGCTCCAGGTAATGCAGGTACAGCGCTTGAACCAAAACTAGAAAACGTCGCTATTGATGTTGAAGATATCG
CTGCCCTTGTTGCTTTTGCAAAAGAAAAAGCCATCGAGCTGACCATCGTTGGTCCTGAAGCACCATTAGTTATTGGCGTT
GTTGATGCATTTCGTGAGGCTGATCTTCCTATCTTTGGACCAACTAAAGCAGCGGCTCAACTTGAAGGTTCTAAAGCCTT
CACCAAAGATTTCTTAGCTCGCCATGACATCCCTACCGGTTCTTATGCAAACTTCACTGAGATTGAACCGGCATTAGCTT
ACGTTCGTGAACAAGGTGCACCTATCGTTGTTAAAGCTGACGGTCTTGCCGCAGGTAAAGGCGTTATCGTTGCGATGACT
CTTGAAGAAGCAGAAGACGCAATCAAAGATATGCTGGCAGGCAATGCGTTTGGTGAAGCAGGTAGCCGTGTTGTTATCGA
AGAATTCCTTGAAGGTGAAGAAGCCAGTTTCATTGTAATGGTGGATGGTGCAAGCGTACTTCCTATGGCAACAAGCCAAG
ATCACAAACGCGTTGGAGATAAAGATACAGGTCCAAATACAGGTGGTATGGGTGCATACTCTCCAGCTCCTGTGGTGACT
CCTGAAATTCATAATCGTATCCTTGAAGAAGTTATCTACCCTACGGTTCGCGGCATGGATGCAGAAGGTGCACCTTATAC
CGGTTTCCTTTATGCAGGCCTTATGATCGATACTGAAGGTACACCAAAGGTAATCGAATATAACTGTCGCTTTGGTGATC
CAGAAACGCAACCTATCATGATGCGTATGGAATCAGACCTTGTTGAACTTTGTCTAATGGCTATCGACGAGAAACTGGAC
GAAGCAGAATCTAAGTGGGACCCACGTGCTTCTATTGGTGTTGTTCTTGCTGCTGGTGGCTATCCAGCGGATTATGCAAA
AGGAGATGTTATTTCGCTGCCTTCAGAAGAAACTGAAGGTCAAAAGATCTTCCATGCAGGCACCACAAATAACGATGCTG
GTGATGTTGTGACAAACGGTGGTCGCGTACTTTGTGCTACCGCATTAGGTAATACCGTATCAGAAGCTCAAGAGCGTGCT
TATGAGCTTGCTAAACAAGTAAGTTGGAATGGAATGTTCCATCGTAATGATATTGGTTATCGTGCTATTGCTCGTGAATT
AGAAACCAAATAG

Upstream 100 bases:

>100_bases
TATTGATAAGGTCAAGGAGAGTTCGCGGAGTTTATAAGTATAAATGAGCAAGCTCGACATAGAGATTAGCAATATGGCGC
AGCTATCAAAGGATAAATAA

Downstream 100 bases:

>100_bases
CTTTACGGAAAATAAATAATAAAGGCGCTGCAATAGCGCCTTTTTTCGTTCGACTTGATTAATCTTAATCTTCAATCAAT
TCCGGGCGCTCTACACATGA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVAIDVEDIAALVAFAKEKAIELTIVGPEAPLVIGV
VDAFREADLPIFGPTKAAAQLEGSKAFTKDFLARHDIPTGSYANFTEIEPALAYVREQGAPIVVKADGLAAGKGVIVAMT
LEEAEDAIKDMLAGNAFGEAGSRVVIEEFLEGEEASFIVMVDGASVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDTEGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLMAIDEKLD
EAESKWDPRASIGVVLAAGGYPADYAKGDVISLPSEETEGQKIFHAGTTNNDAGDVVTNGGRVLCATALGNTVSEAQERA
YELAKQVSWNGMFHRNDIGYRAIARELETK

Sequences:

>Translated_430_residues
MNVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVAIDVEDIAALVAFAKEKAIELTIVGPEAPLVIGV
VDAFREADLPIFGPTKAAAQLEGSKAFTKDFLARHDIPTGSYANFTEIEPALAYVREQGAPIVVKADGLAAGKGVIVAMT
LEEAEDAIKDMLAGNAFGEAGSRVVIEEFLEGEEASFIVMVDGASVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDTEGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLMAIDEKLD
EAESKWDPRASIGVVLAAGGYPADYAKGDVISLPSEETEGQKIFHAGTTNNDAGDVVTNGGRVLCATALGNTVSEAQERA
YELAKQVSWNGMFHRNDIGYRAIARELETK
>Mature_430_residues
MNVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVAIDVEDIAALVAFAKEKAIELTIVGPEAPLVIGV
VDAFREADLPIFGPTKAAAQLEGSKAFTKDFLARHDIPTGSYANFTEIEPALAYVREQGAPIVVKADGLAAGKGVIVAMT
LEEAEDAIKDMLAGNAFGEAGSRVVIEEFLEGEEASFIVMVDGASVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDTEGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLMAIDEKLD
EAESKWDPRASIGVVLAAGGYPADYAKGDVISLPSEETEGQKIFHAGTTNNDAGDVVTNGGRVLCATALGNTVSEAQERA
YELAKQVSWNGMFHRNDIGYRAIARELETK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=49.0610328638498, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=49.0610328638498, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=49.0610328638498, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=49.0610328638498, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=75.4098360655738, Blast_Score=658, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=42.1176470588235, Blast_Score=315, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=43.2735426008969, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=44.6511627906977, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=44.6511627906977, Blast_Score=343, Evalue=9e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45730; Mature: 45730

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVAIDVEDIAALVAFA
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHEEECHHHHHHHHHHH
KEKAIELTIVGPEAPLVIGVVDAFREADLPIFGPTKAAAQLEGSKAFTKDFLARHDIPTG
HCCEEEEEEECCCCCEEEEEHHHHHHCCCCEECCCHHHHHCCCCHHHHHHHHHHCCCCCC
SYANFTEIEPALAYVREQGAPIVVKADGLAAGKGVIVAMTLEEAEDAIKDMLAGNAFGEA
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLEGEEASFIVMVDGASVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
CCEEHHHHHHCCCCCEEEEEECCCCEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDTEGTPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEE
MRMESDLVELCLMAIDEKLDEAESKWDPRASIGVVLAAGGYPADYAKGDVISLPSEETEG
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCC
QKIFHAGTTNNDAGDVVTNGGRVLCATALGNTVSEAQERAYELAKQVSWNGMFHRNDIGY
CEEEECCCCCCCCCCEEECCCEEEEEEHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCCH
RAIARELETK
HHHHHHHCCC
>Mature Secondary Structure
MNVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVAIDVEDIAALVAFA
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHEEECHHHHHHHHHHH
KEKAIELTIVGPEAPLVIGVVDAFREADLPIFGPTKAAAQLEGSKAFTKDFLARHDIPTG
HCCEEEEEEECCCCCEEEEEHHHHHHCCCCEECCCHHHHHCCCCHHHHHHHHHHCCCCCC
SYANFTEIEPALAYVREQGAPIVVKADGLAAGKGVIVAMTLEEAEDAIKDMLAGNAFGEA
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLEGEEASFIVMVDGASVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
CCEEHHHHHHCCCCCEEEEEECCCCEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDTEGTPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEE
MRMESDLVELCLMAIDEKLDEAESKWDPRASIGVVLAAGGYPADYAKGDVISLPSEETEG
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCC
QKIFHAGTTNNDAGDVVTNGGRVLCATALGNTVSEAQERAYELAKQVSWNGMFHRNDIGY
CEEEECCCCCCCCCCEEECCCEEEEEEHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCCH
RAIARELETK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]