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The map label for this gene is purH [H]

Identifier: 59713001

GI number: 59713001

Start: 2680963

End: 2682555

Strand: Direct

Name: purH [H]

Synonym: VF_2394

Alternate gene names: 59713001

Gene position: 2680963-2682555 (Clockwise)

Preceding gene: 59712998

Following gene: 59713002

Centisome position: 92.53

GC content: 47.08

Gene sequence:

>1593_bases
ATGAATAACGCACGTCCGATCCGTCGCGCGCTGATTAGCGTATCTGATAAAACTGGTATTGTAGAATTTGCTCAAGCATT
GGCTGAACGTGGTGTAGATATTTTATCTACCGGCGGTACGGCTCGTCTGCTTGCTGAAAAAGGCATCTCAGTAACTGAAG
TATCTGATTACACTGGCTTCCCTGAAATGATGGATGGCCGCGTTAAAACTCTTCATCCTAAAGTTCATGGTGGTGTACTT
GGTCGTCGCGGTCAAGATGATGACATCATGGAGCAACACGGTATCAATCCTATTGATATGGTTGTTGTTAACCTCTACCC
ATTTGCAGAAACCGTAGCAAAAGAAGGCTGTACCCTTGCTGATGCCGTTGAGAACATCGACATCGGTGGCCCTACTATGG
TTCGCTCTGCAGCGAAAAACCACAAAGACGTAACTATCGTTGTTAACGCTCACGATTACGACCGCGTTATCGCTGAAATG
GACGCGAACGAGAAGTCTCTAACACTAGAAACTCGTTTCGACCTAGCTATCGCAGCATTCGAACACACGGCTTCTTACGA
TGGCATGATCGCAAACTACTTCGGCACGATGGTTCCATCTTACGGTGAGAACAAGGAAGGTGATGAAGAGTCTAAATTCC
CTCGTACGTTCAACCAACAGTTCGAGAAAAAACAAGACATGCGCTACGGTGAGAACAGCCATCAAGCTGCAGCATTCTAC
GTTGAAGCAAATCCTGAAGAAGCATCAGTATCTACTGCTCGTCAAATCCAAGGTAAAGCACTTTCTTACAACAACATCGC
TGACACAGATGCAGCACTTGAGTGTGTGAAAGAGTTCGACGAACCAGCATGTGTGATCGTTAAACACGCTAACCCATGTG
GTGTAGCACTAGGTAAAGACATCCTAGAAGCTTACGACCGTGCATTCAAAACAGACCCAACGTCTGCATTTGGCGGCATC
ATCGCATTCAACCGCGAACTAGACGCAGCAACAGCGACAGCTATCACTGAGCGTCAATTCGTTGAAGTTATCATTGCCCC
TTCAGTTTCTACTGAAGCAGTTGAAATCGTAGCAGCTAAGAAAAATCTTCGCCTACTTGAGTGTGGTGAGTGGACAACTA
AGACGACTGGCTTTGACGTGAAACGCGTTAATGGCGGCTTATTAGTTCAAGACCGTGACCAAGGCATGGTGTCTGAAGAT
GACCTACAAGTTGTTTCTAAACGTCAACCAACAGCAGAAGAGCTAAAAGACGCATTGTTCTGCTGGAAAGTAGCGAAGTA
CGTTAAATCTAACGCTATCGTTTACTCCAAAGGCGACATGACTATCGGTGTGGGCGCTGGCCAAATGAGCCGTGTTTACT
CTGCGAAAATCGCAGGCATCAAAGCTGCAGACGAAGGTCTACAGGTTGAAGGTTGTGTAATGGCATCTGACGCGTTCTTT
CCATTCCGTGACGGTATCGATGCGGCTGCAGAAGCTGGCATCAAATGTGTTATCCAACCGGGCGGCTCAATGCGTGACAA
TGAAGTGATCGAAGCTGCTGATGAACACGGCATGGCGATGATCTTTACGGGTATGCGTCACTTCCGTCACTAA

Upstream 100 bases:

>100_bases
TGGCCAATTGCTGTTTATTGATTCATATGAAATGAAAAATAACAGAACAAGTTCATTTTTGGCAAAAATCCTAACTTAAT
TCTAAAGGAAGAAAGTAGCA

Downstream 100 bases:

>100_bases
TTTTCTCTTCGTATTTGGCATTGTGGCGTCGTTAACTGCTTTCGCCCACCCCAGTCACATAGCGAGCTATGCTCCTGGGG
ATGGGCTCAATTGTTGCCTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MNNARPIRRALISVSDKTGIVEFAQALAERGVDILSTGGTARLLAEKGISVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDDIMEQHGINPIDMVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYDRVIAEM
DANEKSLTLETRFDLAIAAFEHTASYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFDEPACVIVKHANPCGVALGKDILEAYDRAFKTDPTSAFGGI
IAFNRELDAATATAITERQFVEVIIAPSVSTEAVEIVAAKKNLRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSED
DLQVVSKRQPTAEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDNEVIEAADEHGMAMIFTGMRHFRH

Sequences:

>Translated_530_residues
MNNARPIRRALISVSDKTGIVEFAQALAERGVDILSTGGTARLLAEKGISVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDDIMEQHGINPIDMVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYDRVIAEM
DANEKSLTLETRFDLAIAAFEHTASYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFDEPACVIVKHANPCGVALGKDILEAYDRAFKTDPTSAFGGI
IAFNRELDAATATAITERQFVEVIIAPSVSTEAVEIVAAKKNLRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSED
DLQVVSKRQPTAEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDNEVIEAADEHGMAMIFTGMRHFRH
>Mature_530_residues
MNNARPIRRALISVSDKTGIVEFAQALAERGVDILSTGGTARLLAEKGISVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDDIMEQHGINPIDMVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYDRVIAEM
DANEKSLTLETRFDLAIAAFEHTASYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFDEPACVIVKHANPCGVALGKDILEAYDRAFKTDPTSAFGGI
IAFNRELDAATATAITERQFVEVIIAPSVSTEAVEIVAAKKNLRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSED
DLQVVSKRQPTAEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDNEVIEAADEHGMAMIFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=490, Percent_Identity=37.5510204081633, Blast_Score=276, Evalue=5e-74,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=78.7193973634652, Blast_Score=872, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=513, Percent_Identity=32.943469785575, Blast_Score=245, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI71985574, Length=373, Percent_Identity=24.9329758713137, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=36.9230769230769, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323056, Length=618, Percent_Identity=33.1715210355987, Blast_Score=286, Evalue=6e-78,
Organism=Saccharomyces cerevisiae, GI6323768, Length=491, Percent_Identity=35.4378818737271, Blast_Score=259, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24649832, Length=614, Percent_Identity=35.3420195439739, Blast_Score=312, Evalue=3e-85,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57626; Mature: 57626

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNARPIRRALISVSDKTGIVEFAQALAERGVDILSTGGTARLLAEKGISVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEHHHHCCCCEEEECCCCCC
PEMMDGRVKTLHPKVHGGVLGRRGQDDDIMEQHGINPIDMVVVNLYPFAETVAKEGCTLA
HHHHCCCEEEECCHHCCCCCCCCCCCCHHHHHHCCCHHHEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYDRVIAEMDANEKSLTLETRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEECCEEEEEH
EHTASYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
HHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFDEPACVIVKHANPCGVALGKD
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
ILEAYDRAFKTDPTSAFGGIIAFNRELDAATATAITERQFVEVIIAPSVSTEAVEIVAAK
HHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHEEEEEEEECCCCCCCEEEEEEC
KNLRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSEDDLQVVSKRQPTAEELKDALF
CCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHH
CWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
HHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCC
PFRDGIDAAAEAGIKCVIQPGGSMRDNEVIEAADEHGMAMIFTGMRHFRH
CCCCCCCHHHCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEHHHHHHCC
>Mature Secondary Structure
MNNARPIRRALISVSDKTGIVEFAQALAERGVDILSTGGTARLLAEKGISVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEHHHHCCCCEEEECCCCCC
PEMMDGRVKTLHPKVHGGVLGRRGQDDDIMEQHGINPIDMVVVNLYPFAETVAKEGCTLA
HHHHCCCEEEECCHHCCCCCCCCCCCCHHHHHHCCCHHHEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYDRVIAEMDANEKSLTLETRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEECCEEEEEH
EHTASYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
HHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFDEPACVIVKHANPCGVALGKD
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
ILEAYDRAFKTDPTSAFGGIIAFNRELDAATATAITERQFVEVIIAPSVSTEAVEIVAAK
HHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHEEEEEEEECCCCCCCEEEEEEC
KNLRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSEDDLQVVSKRQPTAEELKDALF
CCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHH
CWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
HHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCC
PFRDGIDAAAEAGIKCVIQPGGSMRDNEVIEAADEHGMAMIFTGMRHFRH
CCCCCCCHHHCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA