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The map label for this gene is nagB

Identifier: 59712964

GI number: 59712964

Start: 2645813

End: 2646613

Strand: Direct

Name: nagB

Synonym: VF_2357

Alternate gene names: 59712964

Gene position: 2645813-2646613 (Clockwise)

Preceding gene: 59712963

Following gene: 59712969

Centisome position: 91.31

GC content: 42.7

Gene sequence:

>801_bases
ATGAGATTAATCCCGCTAAACCGCGCTGAACAAGTTGGTGCATGGTCTGCACAACACATTGTTAACCGTATCAATGCATT
CAACCCAACCGCTGATCGTCCATTTGTTCTAGGTCTACCTACTGGCGGTACACCTCTTAATACTTACAAAAAGCTAATCG
AGCTACATAAAGCTGGCGAAGTAAGCTTCAAAAACGTAGTAACGTTCAACATGGATGAGTATGTTGGTCTTCCAGCGGAT
CACCCTGAGTCTTACCGCACATTCATGCACGAAAACTTCTTCAACCACATTGATATCCAACCTGAAAATATCAACCTACT
AAATGGTAATGCAGAAGATCACGAAGCGGAATGTCAACGTTACGAAGACAAAATCAAGTCTTACGGCCGCATTAACCTAT
TCATGGGTGGCGTGGGTAACGACGGTCACATCGCTTTCAATGAACCAGCATCATCTCTATCTTCTCGCACACGTATTAAA
ACGTTAACTGAAGATACTCGTATTGCTAACTCTCGCTTCTTCGGTGGCGACATGAACCTTGTTCCTGAGTACTCACTAAC
AATCGGTGTTGGTACTCTACTTGATTCAGAAGAGATCATGATCCTAATCACAGGCCACAACAAAGGTCTTGCTCTGCAAG
CTGCTGTTGAAGGTTCTGTAAACCACCTATGGACTGTATCTGCACTTCAACTTCACCCTAAATCAGTAATCGTGTGTGAT
GAGCCATCAACACAAGAACTGAAAGTGAAAACAGTTAAATACTTCCAACAGCTTGAAGCTAAAAACATGGAAGGTTTTTA
A

Upstream 100 bases:

>100_bases
CTTTATTGAGCAAAATCATTGGTGATACGTGATAACTTATTTTATAATGCGAATTAATGATTTTTGATTCTACGACTTAC
GTTTTTTTTGAGGTATACAA

Downstream 100 bases:

>100_bases
TTTATTGCATTAATTTCGCAATAAATTAAACAAAAAAACCACCGTTTATCGGTGGTTTTTTTTGTAATAAAAAAAAGCTA
AATATACACAGCTATCTGCG

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRLIPLNRAEQVGAWSAQHIVNRINAFNPTADRPFVLGLPTGGTPLNTYKKLIELHKAGEVSFKNVVTFNMDEYVGLPAD
HPESYRTFMHENFFNHIDIQPENINLLNGNAEDHEAECQRYEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLSSRTRIK
TLTEDTRIANSRFFGGDMNLVPEYSLTIGVGTLLDSEEIMILITGHNKGLALQAAVEGSVNHLWTVSALQLHPKSVIVCD
EPSTQELKVKTVKYFQQLEAKNMEGF

Sequences:

>Translated_266_residues
MRLIPLNRAEQVGAWSAQHIVNRINAFNPTADRPFVLGLPTGGTPLNTYKKLIELHKAGEVSFKNVVTFNMDEYVGLPAD
HPESYRTFMHENFFNHIDIQPENINLLNGNAEDHEAECQRYEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLSSRTRIK
TLTEDTRIANSRFFGGDMNLVPEYSLTIGVGTLLDSEEIMILITGHNKGLALQAAVEGSVNHLWTVSALQLHPKSVIVCD
EPSTQELKVKTVKYFQQLEAKNMEGF
>Mature_266_residues
MRLIPLNRAEQVGAWSAQHIVNRINAFNPTADRPFVLGLPTGGTPLNTYKKLIELHKAGEVSFKNVVTFNMDEYVGLPAD
HPESYRTFMHENFFNHIDIQPENINLLNGNAEDHEAECQRYEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLSSRTRIK
TLTEDTRIANSRFFGGDMNLVPEYSLTIGVGTLLDSEEIMILITGHNKGLALQAAVEGSVNHLWTVSALQLHPKSVIVCD
EPSTQELKVKTVKYFQQLEAKNMEGF

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=258, Percent_Identity=62.015503875969, Blast_Score=341, Evalue=4e-94,
Organism=Homo sapiens, GI19923881, Length=258, Percent_Identity=60.077519379845, Blast_Score=335, Evalue=3e-92,
Organism=Escherichia coli, GI1786893, Length=266, Percent_Identity=75.9398496240601, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1789530, Length=198, Percent_Identity=28.7878787878788, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994958, Length=241, Percent_Identity=25.7261410788382, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554876, Length=258, Percent_Identity=58.1395348837209, Blast_Score=314, Evalue=4e-86,
Organism=Drosophila melanogaster, GI24581960, Length=271, Percent_Identity=58.3025830258303, Blast_Score=341, Evalue=2e-94,
Organism=Drosophila melanogaster, GI19920764, Length=271, Percent_Identity=58.3025830258303, Blast_Score=341, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_VIBF1 (Q5E294)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205740.1
- HSSP:   P0A759
- ProteinModelPortal:   Q5E294
- SMR:   Q5E294
- STRING:   Q5E294
- GeneID:   3277742
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2357
- NMPDR:   fig|312309.3.peg.2357
- eggNOG:   COG0363
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- PhylomeDB:   Q5E294
- ProtClustDB:   PRK00443
- BioCyc:   VFIS312309:VF2357-MONOMER
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 29730; Mature: 29730

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 141-141 ACT_SITE 143-143 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIPLNRAEQVGAWSAQHIVNRINAFNPTADRPFVLGLPTGGTPLNTYKKLIELHKAGE
CEECCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
VSFKNVVTFNMDEYVGLPADHPESYRTFMHENFFNHIDIQPENINLLNGNAEDHEAECQR
CEEEEEEEECHHHHCCCCCCCCHHHHHHHHHCCCCEEECCCCCEEEECCCCCCHHHHHHH
YEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLSSRTRIKTLTEDTRIANSRFFGGDMNL
HHHHHHCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHCCHHHCCCEEECCCCCC
VPEYSLTIGVGTLLDSEEIMILITGHNKGLALQAAVEGSVNHLWTVSALQLHPKSVIVCD
CCCEEEEEEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCEEEEEEEEEECCCEEEEEC
EPSTQELKVKTVKYFQQLEAKNMEGF
CCCCCCEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRLIPLNRAEQVGAWSAQHIVNRINAFNPTADRPFVLGLPTGGTPLNTYKKLIELHKAGE
CEECCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
VSFKNVVTFNMDEYVGLPADHPESYRTFMHENFFNHIDIQPENINLLNGNAEDHEAECQR
CEEEEEEEECHHHHCCCCCCCCHHHHHHHHHCCCCEEECCCCCEEEECCCCCCHHHHHHH
YEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLSSRTRIKTLTEDTRIANSRFFGGDMNL
HHHHHHCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHCCHHHCCCEEECCCCCC
VPEYSLTIGVGTLLDSEEIMILITGHNKGLALQAAVEGSVNHLWTVSALQLHPKSVIVCD
CCCEEEEEEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCEEEEEEEEEECCCEEEEEC
EPSTQELKVKTVKYFQQLEAKNMEGF
CCCCCCEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA