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The map label for this gene is murD [H]

Identifier: 59712810

GI number: 59712810

Start: 2474898

End: 2476112

Strand: Reverse

Name: murD [H]

Synonym: VF_2203

Alternate gene names: 59712810

Gene position: 2476112-2474898 (Counterclockwise)

Preceding gene: 59712811

Following gene: 59712809

Centisome position: 85.46

GC content: 40.58

Gene sequence:

>1215_bases
GTGATTGATACTCGTGATACACCGCCAGGCCATGACCAATTGCCTGAAAATGTAGAACTGCATTCTGGAGGATGGCAACA
AGATTGGCTGATTAACGCTGATCTGATTGTCACGAATCCTGGAATCGCCTTAGCGTCACCTCAACTTAAACCTGCTATTG
ATAAAGGAATTAAAATTGTTGGTGACATCGAGTTATTTGCTTGGGCAGTGAATGCACCTGTTATTGCGATCACCGGGTCT
AATGGCAAAAGTACGGTGACTGATTTAACTGGGGAAATGGCAAAAGCGGCTGGAGTAAAAACAGCCGTTGGTGGAAACAT
TGGTTTTGCTGCTCTTGATTTACTAGAGCAAGATGCGGAGCTTTATGTTCTTGAGCTTTCGAGTTTCCAACTTGAAACAA
CATCCACATTAAAGCTGAAAGCCGCTGCCTTTCTTAATTTGTCTGAAGATCATATGGATCGTTATCAAGGTATGGCGGAT
TATCGCCAAGCTAAGCTACGTATCTTTGAGCATGCCGAAGTATGTATTGTTAATCGTGACGATAAACAAACTTATCCTGA
CGTTGAAAAAACATTAAAAAGCTTTGGGTTTGACCAAGGCGATTATGGCTGTATTGAAAAAGATGGCATTGAGTATTTAG
CTAAAAATACAGTGCCATTGCTTGCTGCAAATGAATTAGGCTTAGTCGGAAAGCACAATATTGCAAATAGCCTAGTTGCA
ATTGCATTACTTGATGCCGCTGGTATCAATTTAGATGCAACACTAGATACATTAAGAACTTACAATGGATTGACACATAG
ATGCCAAGTTGTCGCAGATAATAATGGTATTCGCTGGGTCAATGATTCAAAAGCGACAAACGTAGCAAGTACATTGGCAG
CCTTATCTGGTTTACAACTTGAAGGTAAATTACACCTACTTGTTGGTGGTGTAGGCAAAGGTGCTGATTTTTCAGAATTA
TCACCAGCGCTGCATGATTTAAATTTAATGATGTATTGCTTTGGTGAAGATGGCGACCAATTTGTATCACTTGATCCTCG
TTCACTCTTATGTGAAACCATGAATGAAGCCATCGCTACTCTATACCCGACTTTAAATAAAGGCGATATGGTGATGCTAT
CTCCAGCATGTGCAAGCTTCGACCAATACGCAAATTTTATGGCTAGAGGGGATGCATTCACCCAGTTAGCAAAACAGTAT
TCAATTGAGAGTTAA

Upstream 100 bases:

>100_bases
TTTGGTGGTGTAAAAAATGTTGTAGTAGTTGGCTTAGGTATGACAGGTTTATCTGTTGTTAAGCATTTACTTAAGACTAC
CATAAGCGTTAACAATTAAA

Downstream 100 bases:

>100_bases
TTTTGGCATTTGAGCGAATTAAACAAGGGATCTTTTCCATTCAAGATTGGTGTTTAACACCGTCACCTAAAGTCATGTTC
GATCGTCAGTTGATATGGAT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MIDTRDTPPGHDQLPENVELHSGGWQQDWLINADLIVTNPGIALASPQLKPAIDKGIKIVGDIELFAWAVNAPVIAITGS
NGKSTVTDLTGEMAKAAGVKTAVGGNIGFAALDLLEQDAELYVLELSSFQLETTSTLKLKAAAFLNLSEDHMDRYQGMAD
YRQAKLRIFEHAEVCIVNRDDKQTYPDVEKTLKSFGFDQGDYGCIEKDGIEYLAKNTVPLLAANELGLVGKHNIANSLVA
IALLDAAGINLDATLDTLRTYNGLTHRCQVVADNNGIRWVNDSKATNVASTLAALSGLQLEGKLHLLVGGVGKGADFSEL
SPALHDLNLMMYCFGEDGDQFVSLDPRSLLCETMNEAIATLYPTLNKGDMVMLSPACASFDQYANFMARGDAFTQLAKQY
SIES

Sequences:

>Translated_404_residues
MIDTRDTPPGHDQLPENVELHSGGWQQDWLINADLIVTNPGIALASPQLKPAIDKGIKIVGDIELFAWAVNAPVIAITGS
NGKSTVTDLTGEMAKAAGVKTAVGGNIGFAALDLLEQDAELYVLELSSFQLETTSTLKLKAAAFLNLSEDHMDRYQGMAD
YRQAKLRIFEHAEVCIVNRDDKQTYPDVEKTLKSFGFDQGDYGCIEKDGIEYLAKNTVPLLAANELGLVGKHNIANSLVA
IALLDAAGINLDATLDTLRTYNGLTHRCQVVADNNGIRWVNDSKATNVASTLAALSGLQLEGKLHLLVGGVGKGADFSEL
SPALHDLNLMMYCFGEDGDQFVSLDPRSLLCETMNEAIATLYPTLNKGDMVMLSPACASFDQYANFMARGDAFTQLAKQY
SIES
>Mature_404_residues
MIDTRDTPPGHDQLPENVELHSGGWQQDWLINADLIVTNPGIALASPQLKPAIDKGIKIVGDIELFAWAVNAPVIAITGS
NGKSTVTDLTGEMAKAAGVKTAVGGNIGFAALDLLEQDAELYVLELSSFQLETTSTLKLKAAAFLNLSEDHMDRYQGMAD
YRQAKLRIFEHAEVCIVNRDDKQTYPDVEKTLKSFGFDQGDYGCIEKDGIEYLAKNTVPLLAANELGLVGKHNIANSLVA
IALLDAAGINLDATLDTLRTYNGLTHRCQVVADNNGIRWVNDSKATNVASTLAALSGLQLEGKLHLLVGGVGKGADFSEL
SPALHDLNLMMYCFGEDGDQFVSLDPRSLLCETMNEAIATLYPTLNKGDMVMLSPACASFDQYANFMARGDAFTQLAKQY
SIES

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=403, Percent_Identity=55.8312655086849, Blast_Score=417, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 43606; Mature: 43606

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTRDTPPGHDQLPENVELHSGGWQQDWLINADLIVTNPGIALASPQLKPAIDKGIKIV
CCCCCCCCCCCCCCCCCEEECCCCCCCCEEEECEEEEECCCCEEECCCCCHHHHCCCEEE
GDIELFAWAVNAPVIAITGSNGKSTVTDLTGEMAKAAGVKTAVGGNIGFAALDLLEQDAE
EEEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCE
LYVLELSSFQLETTSTLKLKAAAFLNLSEDHMDRYQGMADYRQAKLRIFEHAEVCIVNRD
EEEEEECCEEEECCCEEEEEEEEEECCCHHHHHHHCCHHHHHHHHHEEEECCEEEEEECC
DKQTYPDVEKTLKSFGFDQGDYGCIEKDGIEYLAKNTVPLLAANELGLVGKHNIANSLVA
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHHHH
IALLDAAGINLDATLDTLRTYNGLTHRCQVVADNNGIRWVNDSKATNVASTLAALSGLQL
HHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCEE
EGKLHLLVGGVGKGADFSELSPALHDLNLMMYCFGEDGDQFVSLDPRSLLCETMNEAIAT
CCEEEEEEECCCCCCCHHHHCHHHHCCEEEEEEECCCCCCEEEECHHHHHHHHHHHHHHH
LYPTLNKGDMVMLSPACASFDQYANFMARGDAFTQLAKQYSIES
HCCCCCCCCEEEECHHHHCHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIDTRDTPPGHDQLPENVELHSGGWQQDWLINADLIVTNPGIALASPQLKPAIDKGIKIV
CCCCCCCCCCCCCCCCCEEECCCCCCCCEEEECEEEEECCCCEEECCCCCHHHHCCCEEE
GDIELFAWAVNAPVIAITGSNGKSTVTDLTGEMAKAAGVKTAVGGNIGFAALDLLEQDAE
EEEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCE
LYVLELSSFQLETTSTLKLKAAAFLNLSEDHMDRYQGMADYRQAKLRIFEHAEVCIVNRD
EEEEEECCEEEECCCEEEEEEEEEECCCHHHHHHHCCHHHHHHHHHEEEECCEEEEEECC
DKQTYPDVEKTLKSFGFDQGDYGCIEKDGIEYLAKNTVPLLAANELGLVGKHNIANSLVA
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHHHH
IALLDAAGINLDATLDTLRTYNGLTHRCQVVADNNGIRWVNDSKATNVASTLAALSGLQL
HHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCEE
EGKLHLLVGGVGKGADFSELSPALHDLNLMMYCFGEDGDQFVSLDPRSLLCETMNEAIAT
CCEEEEEEECCCCCCCHHHHCHHHHCCEEEEEEECCCCCCEEEECHHHHHHHHHHHHHHH
LYPTLNKGDMVMLSPACASFDQYANFMARGDAFTQLAKQYSIES
HCCCCCCCCEEEECHHHHCHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA