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The map label for this gene is pilD [H]

Identifier: 59712795

GI number: 59712795

Start: 2459978

End: 2460865

Strand: Direct

Name: pilD [H]

Synonym: VF_2188

Alternate gene names: 59712795

Gene position: 2459978-2460865 (Clockwise)

Preceding gene: 59712794

Following gene: 59712796

Centisome position: 84.9

GC content: 39.64

Gene sequence:

>888_bases
ATGGCTGTTTTTGATTATTACCCTTGGCTTTTCCCTCTATTTGCCACCCTATTTGGGTTGCTTGTTGGTAGTTTTTTAAA
TGTCGTAATTTATCGTTTACCCATCATGATGGAAAAGGAATGGAAAAAAGACTGCATTGATTGCTTTCCAGATCTTGCCC
CTAAAAAAACAAGTGAAGAAAAAGAAGAAACCTTTAACTTAAGTGTTCCTCGCTCTCGCTGCCCAAAGTGTCAAACTCAA
ATTAAATTCTATGACAACATTCCTGTCATTAGCTGGCTTTTATTAAAAGGAAAATGTCGTCAATGCAGTAACCCTATCAG
TTTTCGATATCCTGCAATTGAACTACTATCAGGCGCAATGTGTTTTGCCGTTTCATACTTATTACCCTTCTCATATTTTG
CTGTTGCTGCCGTTCTATTTACTCTAGTTTTGATCGCATTAACCTTTATCGATATTGATACCATGTTACTGCCAGATCAA
ATCACCCTACCTCTACTATGGAGTGGTCTATACTTAGCTCTAGTGGGTTGGAGTTCAGTGAGCTTAGTTGATTCTGTTGT
TGGTGCAATGGCTGGCTATTTGATTTTATGGTCTATTTACTGGGGCTTTAAGCTGCTTACAGGCAAAGAAGGCATGGGTT
ATGGTGATTTCAAATTACTGGCGGCTCTTGGAGCATGGCTTGGTTGGCAGCAGCTACCATTGATCATTCTATTATCTTCT
GTTGTCGGAGCAATCATTGGCGTCATCATGCTAACCGCTCAAAAGAAAGGGTTTGACAAAGCAATTCCCTTTGGTCCTTA
TTTAGCTATTGCTGGCTGGGTTTGCCTATTATGGGGACAAAACATTAGCCAATGGTATTTTAATCACATTCTAGGTTTGC
CACTATGA

Upstream 100 bases:

>100_bases
TGTAATAAGTGCGTTAAGTTTATACTCTTAACTACTTCATTTTAATGAATTCTGATACCATCAGCTAAATACGTTAATTA
CTGATATTGGACGCTTTTAC

Downstream 100 bases:

>100_bases
GTTATGTCGTAGCAATTACTGGTGGTATTGGTAGTGGAAAAACTACCGTTGCCGATCGCTTTCAAGCACTCTATAACATC
AATATTGTTGATGCTGATAT

Product: type 4 prepilin peptidase PilD

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAVFDYYPWLFPLFATLFGLLVGSFLNVVIYRLPIMMEKEWKKDCIDCFPDLAPKKTSEEKEETFNLSVPRSRCPKCQTQ
IKFYDNIPVISWLLLKGKCRQCSNPISFRYPAIELLSGAMCFAVSYLLPFSYFAVAAVLFTLVLIALTFIDIDTMLLPDQ
ITLPLLWSGLYLALVGWSSVSLVDSVVGAMAGYLILWSIYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPLIILLSS
VVGAIIGVIMLTAQKKGFDKAIPFGPYLAIAGWVCLLWGQNISQWYFNHILGLPL

Sequences:

>Translated_295_residues
MAVFDYYPWLFPLFATLFGLLVGSFLNVVIYRLPIMMEKEWKKDCIDCFPDLAPKKTSEEKEETFNLSVPRSRCPKCQTQ
IKFYDNIPVISWLLLKGKCRQCSNPISFRYPAIELLSGAMCFAVSYLLPFSYFAVAAVLFTLVLIALTFIDIDTMLLPDQ
ITLPLLWSGLYLALVGWSSVSLVDSVVGAMAGYLILWSIYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPLIILLSS
VVGAIIGVIMLTAQKKGFDKAIPFGPYLAIAGWVCLLWGQNISQWYFNHILGLPL
>Mature_294_residues
AVFDYYPWLFPLFATLFGLLVGSFLNVVIYRLPIMMEKEWKKDCIDCFPDLAPKKTSEEKEETFNLSVPRSRCPKCQTQI
KFYDNIPVISWLLLKGKCRQCSNPISFRYPAIELLSGAMCFAVSYLLPFSYFAVAAVLFTLVLIALTFIDIDTMLLPDQI
TLPLLWSGLYLALVGWSSVSLVDSVVGAMAGYLILWSIYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPLIILLSSV
VGAIIGVIMLTAQKKGFDKAIPFGPYLAIAGWVCLLWGQNISQWYFNHILGLPL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=270, Percent_Identity=39.6296296296296, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1789732, Length=149, Percent_Identity=44.9664429530201, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 33133; Mature: 33002

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFDYYPWLFPLFATLFGLLVGSFLNVVIYRLPIMMEKEWKKDCIDCFPDLAPKKTSEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
KEETFNLSVPRSRCPKCQTQIKFYDNIPVISWLLLKGKCRQCSNPISFRYPAIELLSGAM
HHHHCCCCCCHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
CFAVSYLLPFSYFAVAAVLFTLVLIALTFIDIDTMLLPDQITLPLLWSGLYLALVGWSSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHH
SLVDSVVGAMAGYLILWSIYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPLIILLSS
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
VVGAIIGVIMLTAQKKGFDKAIPFGPYLAIAGWVCLLWGQNISQWYFNHILGLPL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
AVFDYYPWLFPLFATLFGLLVGSFLNVVIYRLPIMMEKEWKKDCIDCFPDLAPKKTSEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
KEETFNLSVPRSRCPKCQTQIKFYDNIPVISWLLLKGKCRQCSNPISFRYPAIELLSGAM
HHHHCCCCCCHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
CFAVSYLLPFSYFAVAAVLFTLVLIALTFIDIDTMLLPDQITLPLLWSGLYLALVGWSSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHH
SLVDSVVGAMAGYLILWSIYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPLIILLSS
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
VVGAIIGVIMLTAQKKGFDKAIPFGPYLAIAGWVCLLWGQNISQWYFNHILGLPL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9826339 [H]