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The map label for this gene is ispU

Identifier: 59712565

GI number: 59712565

Start: 2188643

End: 2189416

Strand: Reverse

Name: ispU

Synonym: VF_1958

Alternate gene names: 59712565

Gene position: 2189416-2188643 (Counterclockwise)

Preceding gene: 59712566

Following gene: 59712564

Centisome position: 75.56

GC content: 38.76

Gene sequence:

>774_bases
ATGACCAGTCAAATTAATAACGAGTTTTTATCATTAGATGTATTACCAAAACATATTGCGGTAATTATGGATGGTAATGG
ACGCTGGGCTAAGGCTCAGGGAAAACCTCGTGTATTTGGTCATAAAGCGGGTGTTGATGCGGTAAGAAAAACCATTTCAG
CAGCCAGTAAGCTTGGGATCAATGCCATAACACTTTTTGCTTTTAGTAGTGAGAATTGGCGTCGACCTGAAGAAGAAGTA
AGTGTTCTAATGGAGTTGTTTATCACGGTACTTTCTCGTGAAGTAAAACGTCTTCATAAAAATAATATTCAATTGCGTAT
AATTGGTGAAACCTCTCAGTTTAGTTCTCGCCTTCAGAAGAAAATTGTTGAAGCGGAAACATTAACTTCTGAAAATACAG
GGTTAGTACTAAACGTTGCTGCAAACTATGGTGGCAAATGGGATATTACACAAGCAGTACGTCGCCTCGCATATCAAGTG
GAACGAGGCAATTTACAGGCAGATGATATTGATGAGGATCTTATCGCATCTAAACTGACTATGTCAGATTTGCCTGAAGT
GGATTTGATGATTAGAACGAGCGGTGAATGTCGTATTAGCAACTTTATGTTGTGGCAAATGGCTTATGCTGAATTCTATT
TCACTGACCAATATTGGCCTGATTTTAATGAACAAAGCTTAGCTGACGCTGTTGCTTGGTTTGTTAGTCGAGAAAGACGA
TTTGGTTGTACTGGTGAACAAATCCAAGCTCTAATGAACGAAAAGGATCAATAA

Upstream 100 bases:

>100_bases
TCACTGATCTTTTCATTAAATTAATGATATGATGTCGGCGACGAACACCATAAGAAGCGCTGGGTGGAGACATCAGCGCT
TTTATTTTTAAGGGAGTTAT

Downstream 100 bases:

>100_bases
AGTTTGAAACAGCGTATTATTACTGCTCTTATTTTGGCGCCCCTTGTTATTGCAGGGATCTTCATGTTGCCAATGCAAGG
GTTCTTAATTGCTATCACAG

Product: undecaprenyl pyrophosphate synthase

Products: NA

Alternate protein names: UPP synthase; Di-trans,poly-cis-decaprenylcistransferase; Undecaprenyl diphosphate synthase; UDS

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTSQINNEFLSLDVLPKHIAVIMDGNGRWAKAQGKPRVFGHKAGVDAVRKTISAASKLGINAITLFAFSSENWRRPEEEV
SVLMELFITVLSREVKRLHKNNIQLRIIGETSQFSSRLQKKIVEAETLTSENTGLVLNVAANYGGKWDITQAVRRLAYQV
ERGNLQADDIDEDLIASKLTMSDLPEVDLMIRTSGECRISNFMLWQMAYAEFYFTDQYWPDFNEQSLADAVAWFVSRERR
FGCTGEQIQALMNEKDQ

Sequences:

>Translated_257_residues
MTSQINNEFLSLDVLPKHIAVIMDGNGRWAKAQGKPRVFGHKAGVDAVRKTISAASKLGINAITLFAFSSENWRRPEEEV
SVLMELFITVLSREVKRLHKNNIQLRIIGETSQFSSRLQKKIVEAETLTSENTGLVLNVAANYGGKWDITQAVRRLAYQV
ERGNLQADDIDEDLIASKLTMSDLPEVDLMIRTSGECRISNFMLWQMAYAEFYFTDQYWPDFNEQSLADAVAWFVSRERR
FGCTGEQIQALMNEKDQ
>Mature_256_residues
TSQINNEFLSLDVLPKHIAVIMDGNGRWAKAQGKPRVFGHKAGVDAVRKTISAASKLGINAITLFAFSSENWRRPEEEVS
VLMELFITVLSREVKRLHKNNIQLRIIGETSQFSSRLQKKIVEAETLTSENTGLVLNVAANYGGKWDITQAVRRLAYQVE
RGNLQADDIDEDLIASKLTMSDLPEVDLMIRTSGECRISNFMLWQMAYAEFYFTDQYWPDFNEQSLADAVAWFVSRERRF
GCTGEQIQALMNEKDQ

Specific function: Generates undecaprenyl pyrophosphate (UPP) from isopentenyl pyrophosphate (IPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide

COG id: COG0020

COG function: function code I; Undecaprenyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPP synthase family

Homologues:

Organism=Homo sapiens, GI45580738, Length=223, Percent_Identity=37.219730941704, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI45580742, Length=223, Percent_Identity=37.219730941704, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1786371, Length=228, Percent_Identity=61.8421052631579, Blast_Score=308, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI71993029, Length=229, Percent_Identity=36.2445414847162, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323748, Length=226, Percent_Identity=32.3008849557522, Blast_Score=116, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319474, Length=221, Percent_Identity=30.7692307692308, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18857969, Length=209, Percent_Identity=36.3636363636364, Blast_Score=137, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UPPS_VIBF1 (Q5E3E3)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205341.1
- ProteinModelPortal:   Q5E3E3
- SMR:   Q5E3E3
- STRING:   Q5E3E3
- GeneID:   3279225
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1958
- NMPDR:   fig|312309.3.peg.1958
- eggNOG:   COG0020
- HOGENOM:   HBG627837
- OMA:   FLWRIAY
- PhylomeDB:   Q5E3E3
- ProtClustDB:   CLSK2517517
- BioCyc:   VFIS312309:VF1958-MONOMER
- HAMAP:   MF_01139
- InterPro:   IPR001441
- InterPro:   IPR018520
- Gene3D:   G3DSA:3.40.1180.10
- PANTHER:   PTHR10291
- TIGRFAMs:   TIGR00055

Pfam domain/function: PF01255 Prenyltransf; SSF64005 UPP_synth

EC number: =2.5.1.31

Molecular weight: Translated: 29233; Mature: 29101

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01066 UPP_SYNTHASE

Important sites: ACT_SITE 24-24

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQINNEFLSLDVLPKHIAVIMDGNGRWAKAQGKPRVFGHKAGVDAVRKTISAASKLGI
CCCCCCCCEEEEEECCCEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCC
NAITLFAFSSENWRRPEEEVSVLMELFITVLSREVKRLHKNNIQLRIIGETSQFSSRLQK
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHH
KIVEAETLTSENTGLVLNVAANYGGKWDITQAVRRLAYQVERGNLQADDIDEDLIASKLT
HHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
MSDLPEVDLMIRTSGECRISNFMLWQMAYAEFYFTDQYWPDFNEQSLADAVAWFVSRERR
HCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCC
FGCTGEQIQALMNEKDQ
CCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TSQINNEFLSLDVLPKHIAVIMDGNGRWAKAQGKPRVFGHKAGVDAVRKTISAASKLGI
CCCCCCCEEEEEECCCEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCC
NAITLFAFSSENWRRPEEEVSVLMELFITVLSREVKRLHKNNIQLRIIGETSQFSSRLQK
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHH
KIVEAETLTSENTGLVLNVAANYGGKWDITQAVRRLAYQVERGNLQADDIDEDLIASKLT
HHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
MSDLPEVDLMIRTSGECRISNFMLWQMAYAEFYFTDQYWPDFNEQSLADAVAWFVSRERR
HCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCC
FGCTGEQIQALMNEKDQ
CCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA