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The map label for this gene is aat

Identifier: 59712369

GI number: 59712369

Start: 1988971

End: 1989690

Strand: Direct

Name: aat

Synonym: VF_1762

Alternate gene names: 59712369

Gene position: 1988971-1989690 (Clockwise)

Preceding gene: 59712361

Following gene: 59712370

Centisome position: 68.64

GC content: 36.53

Gene sequence:

>720_bases
ATGACTATCTACTTACCTGAGCTTATTCCATCTCATTCGACAGTTTTTCCTGATATAGAGAACGCATTAAATAACCCAGA
TGGATTACTCATTATGGGAGGGGACTTATCTTCCAAACAACTAATTAGTGCATATCAACACGGTATTTTCCCTTGGTACA
GTAATGGAGATCCTATACTTTGGTGGAGTCCAAGCGTTCGAGGCGTTTTTTTCCCTGAGCAATTTTCACCATCTAAAAGC
TTAAAAAAGTTCTTCAGAAAATCGAACTACAACGTAACCCTTAATAAAGCCACTTACCAAGTTATTGACCTCTGTGCATC
TACCAGACCAAAAGAAGAAACATGGATCATGCCAGAAATGATCAATGCCTATAAGAAACTGGCTAATCTTGGCTATTGTC
ACTCTGTTGAGGTATGGAATGAGGATGAATTAATTGGTGGTTTATATGGATTACAAATCGGTCAAATTTTTTGTGGTGAG
TCTATGTTTTCATTGCAAACAAACGCATCCAAAATTGCATTATGGAAATTTTGCGAACATTTTGTATCATTCAATGGAAA
GCTTATTGACTGCCAAATGATGAATCCTCATTTAGAGAGTCTTGGGGCCAAAGAAATGAAACGCTGTGATTTCAAAACAT
TACTTGAAGAACTCAGCACTAAATCAACAATAGCAAACTGTTACTTACCTCAAATATTAGGGGATAATTCACTTTCATGA

Upstream 100 bases:

>100_bases
TATTCTTCAATACTTTCAGAGTAACGAAAACCAACGCGATGTGCCAGAGGCAATATCGTCTCTTTGACCTAAAAATAATG
AATAGTGATTTCTTTTATCT

Downstream 100 bases:

>100_bases
GTCAGTATTCACTTCAAGTTGGTTTAACACCCGAAGGGCCTTGTAGCTACCTACCTAAACAAAAAGAAAGACTCGCTGTT
GTAATGGAACAAGAATTACA

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTIYLPELIPSHSTVFPDIENALNNPDGLLIMGGDLSSKQLISAYQHGIFPWYSNGDPILWWSPSVRGVFFPEQFSPSKS
LKKFFRKSNYNVTLNKATYQVIDLCASTRPKEETWIMPEMINAYKKLANLGYCHSVEVWNEDELIGGLYGLQIGQIFCGE
SMFSLQTNASKIALWKFCEHFVSFNGKLIDCQMMNPHLESLGAKEMKRCDFKTLLEELSTKSTIANCYLPQILGDNSLS

Sequences:

>Translated_239_residues
MTIYLPELIPSHSTVFPDIENALNNPDGLLIMGGDLSSKQLISAYQHGIFPWYSNGDPILWWSPSVRGVFFPEQFSPSKS
LKKFFRKSNYNVTLNKATYQVIDLCASTRPKEETWIMPEMINAYKKLANLGYCHSVEVWNEDELIGGLYGLQIGQIFCGE
SMFSLQTNASKIALWKFCEHFVSFNGKLIDCQMMNPHLESLGAKEMKRCDFKTLLEELSTKSTIANCYLPQILGDNSLS
>Mature_238_residues
TIYLPELIPSHSTVFPDIENALNNPDGLLIMGGDLSSKQLISAYQHGIFPWYSNGDPILWWSPSVRGVFFPEQFSPSKSL
KKFFRKSNYNVTLNKATYQVIDLCASTRPKEETWIMPEMINAYKKLANLGYCHSVEVWNEDELIGGLYGLQIGQIFCGES
MFSLQTNASKIALWKFCEHFVSFNGKLIDCQMMNPHLESLGAKEMKRCDFKTLLEELSTKSTIANCYLPQILGDNSLS

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=225, Percent_Identity=52, Blast_Score=245, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_VIBF1 (Q5E3Y9)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205145.1
- HSSP:   P0A8P1
- ProteinModelPortal:   Q5E3Y9
- SMR:   Q5E3Y9
- STRING:   Q5E3Y9
- GeneID:   3279021
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1762
- NMPDR:   fig|312309.3.peg.1762
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- PhylomeDB:   Q5E3Y9
- ProtClustDB:   PRK00301
- BioCyc:   VFIS312309:VF1762-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 27102; Mature: 26971

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIYLPELIPSHSTVFPDIENALNNPDGLLIMGGDLSSKQLISAYQHGIFPWYSNGDPIL
CEEECCCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCEECCCCEEE
WWSPSVRGVFFPEQFSPSKSLKKFFRKSNYNVTLNKATYQVIDLCASTRPKEETWIMPEM
EECCCCCEEECCCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCCCCEECHHH
INAYKKLANLGYCHSVEVWNEDELIGGLYGLQIGQIFCGESMFSLQTNASKIALWKFCEH
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHH
FVSFNGKLIDCQMMNPHLESLGAKEMKRCDFKTLLEELSTKSTIANCYLPQILGDNSLS
HHCCCCEEEEEEECCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TIYLPELIPSHSTVFPDIENALNNPDGLLIMGGDLSSKQLISAYQHGIFPWYSNGDPIL
EEECCCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCEECCCCEEE
WWSPSVRGVFFPEQFSPSKSLKKFFRKSNYNVTLNKATYQVIDLCASTRPKEETWIMPEM
EECCCCCEEECCCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCCCCEECHHH
INAYKKLANLGYCHSVEVWNEDELIGGLYGLQIGQIFCGESMFSLQTNASKIALWKFCEH
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHH
FVSFNGKLIDCQMMNPHLESLGAKEMKRCDFKTLLEELSTKSTIANCYLPQILGDNSLS
HHCCCCEEEEEEECCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA