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The map label for this gene is metG [H]

Identifier: 59712361

GI number: 59712361

Start: 1979961

End: 1982021

Strand: Direct

Name: metG [H]

Synonym: VF_1754

Alternate gene names: 59712361

Gene position: 1979961-1982021 (Clockwise)

Preceding gene: 59712357

Following gene: 59712369

Centisome position: 68.33

GC content: 41.05

Gene sequence:

>2061_bases
ATGGCTACAGATCCAAGAAAAATTCTGGTTACATGTGCCCTACCGTATGCAAACGGTTCAATCCACTTAGGCCACATGCT
TGAGCATATCCAAGCGGATATCTGGGTTCGTTACCAACGTTTACGCGGCAACGACGTTAACTTTATTTGTGCAGATGATG
CTCACGGCACACCAATCATGCTTAAAGCTCAACAGATGGGTATTTCTCCAGAAGAAATGATCGCAGCCGTTAGCGAAGAG
CACCAAAAAGATTTCGCTGGCTTTGATATCAGCTTTGATAACTATCACAGCACGCACAGTGATGAAAACCGCGAATTAGC
TTCTCACATCTACCTTGAGCTAAAGAAAAACGGCTTTATTACAAGTCGCACAATTTCTCAGCTGTTCGATCCAGAAAAAG
AGATGTTCTTACCTGATCGTTTCGTAAAAGGTACTTGTCCTAAATGTAAAGCAGAAGACCAATACGGTGATAACTGCGAC
AACTGTGGCGAAACATACAGCCCAACAGATTTAATTAATCCAAAATCAGCCGTTTCTGGTGCGACTCCAGTAATGAAAGA
TTCTGAGCATTTCTTCTTTGACCTGCCTCAGTTTGAAAGCATGCTTAAAGAGTGGACTCGTTCTGGTTCTCTACAATCAG
AAACAGCAAACAAGATGCAAGAGTGGTTTGAATCAGGCCTACAACAATGGGATATCTCTCGTGATGCACCATACTTTGGC
TTTGAGATCCCAGGCGAAACGAATAAATTCTTCTACGTTTGGTTAGATGCTCCGATTGGTTATATGGGCTCATTCAAAAA
CCTATGTAATAAGCGTGACGATTTAAACTTTGATGAATACTGGAAGAAAGACAGTACAACAGAGCTTTATCACTTCATTG
GTAAAGACATTGTTTACTTCCACAGTCTATTCTGGCCTGCAATGCTTGATGGCGCAGGTTTCCGTAAACCAAACAACGTA
TTCGTTCACGGTTATGTAACGGTTAACGGTGCGAAAATGTCTAAATCTAAAGGCACTTTCATTAAAGCTGGTACTTACTT
AAATCACTTAGATCCTGAGTGTCTACGTTACTACTATGCAGCGAAACTAAACAGCCGTATTGATGATCTTGATTTAAACC
TTGAAGATTTCACTCAACGTGTAAACTCTGATGTAGTTAACAAGATTGTTAACCTAGCTTCACGTAACGCTGGTTTCATT
ACTAAACGCTTTGATGGCAAACTTGCAGATAACTTTGTAGAGCCTGAGCTTTACAATGAATTTATTGCGGCTGCAGATCG
TATTGCTGAGCTATATGAAACGCGTGAATTTGGTCGTGCTATCCGTGAAATCACAGCACTAGCTGATAAAGCAAACCAAT
ACATTGATGAGAAAGCACCTTGGGTTCTTGCAAAAGAAGAAGGGAAAGAACAAGAACTACAAGAAGTGTCTTCTGTAGGT
ATTAACTTGTTCCGTGTATTAATGGCTTACTTAAAACCTGTAATGCCAGAGCTTGCAGCTCGTACTGAAGCGTTCTTAAA
TGAAACACTAACGTGGGAAGGCGTCGCACAGCCACTAGTTGCTCATGAGATCACTAAGTTTAAAGCATTATTCGCACGTA
TTGATCCTAAGAAAGTTGAAGCTATGATTGAAGAGTCTAAAGAAGACGCAGCAATCGAAATGGCAGCAAAAGAAAAGGCA
GAAGCGGAAAAAGAAAAAGCAAGTCAAACTGAGCTAGATAAAGATCCAATCGCAGATGAGATTGAGTTCGATGCATTCGA
AGCGGTTGATATGCGTATTGCTCGTATTATCTCTTGTGAGGAAGTACCAAAGGCAAACAAACTGCTTAAATTCCAACTAG
ACATCGGTGGCGAAACTCGTCAAGTATTCTCTGGTATTAAATCAGCATATAAACCTGAAGAGCTAGAAGGCAAGCTAACT
GTTATGGTTGCTAACCTAAAACCTCGTAAAATGAAATTTGGTATGTCTGAAGGTATGATCCTAGCTGCAGGCCCTGGTGG
TAAAGAGCTTTGGATTTTAGAACCACACGAAGGCGCTCAACCTGGTATGCGTGTAATGTAA

Upstream 100 bases:

>100_bases
TATCACTAATTGAACCCTTGATCCTCTATGCTTTCTAACTTGGTTCAGGTACTATTTATCTCTTAAATTTTCACCCATCA
CATTAAGCGAAAGAGAAGAT

Downstream 100 bases:

>100_bases
TTCTTCTTTTTAAAAGATAATACCAGTCGTAGTAAATCAATTACTTACTGTGATTGGTATAACTTACAAACAAAAATAGA
GACCAAATGGTCTCTATTTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MATDPRKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNDVNFICADDAHGTPIMLKAQQMGISPEEMIAAVSEE
HQKDFAGFDISFDNYHSTHSDENRELASHIYLELKKNGFITSRTISQLFDPEKEMFLPDRFVKGTCPKCKAEDQYGDNCD
NCGETYSPTDLINPKSAVSGATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQSETANKMQEWFESGLQQWDISRDAPYFG
FEIPGETNKFFYVWLDAPIGYMGSFKNLCNKRDDLNFDEYWKKDSTTELYHFIGKDIVYFHSLFWPAMLDGAGFRKPNNV
FVHGYVTVNGAKMSKSKGTFIKAGTYLNHLDPECLRYYYAAKLNSRIDDLDLNLEDFTQRVNSDVVNKIVNLASRNAGFI
TKRFDGKLADNFVEPELYNEFIAAADRIAELYETREFGRAIREITALADKANQYIDEKAPWVLAKEEGKEQELQEVSSVG
INLFRVLMAYLKPVMPELAARTEAFLNETLTWEGVAQPLVAHEITKFKALFARIDPKKVEAMIEESKEDAAIEMAAKEKA
EAEKEKASQTELDKDPIADEIEFDAFEAVDMRIARIISCEEVPKANKLLKFQLDIGGETRQVFSGIKSAYKPEELEGKLT
VMVANLKPRKMKFGMSEGMILAAGPGGKELWILEPHEGAQPGMRVM

Sequences:

>Translated_686_residues
MATDPRKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNDVNFICADDAHGTPIMLKAQQMGISPEEMIAAVSEE
HQKDFAGFDISFDNYHSTHSDENRELASHIYLELKKNGFITSRTISQLFDPEKEMFLPDRFVKGTCPKCKAEDQYGDNCD
NCGETYSPTDLINPKSAVSGATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQSETANKMQEWFESGLQQWDISRDAPYFG
FEIPGETNKFFYVWLDAPIGYMGSFKNLCNKRDDLNFDEYWKKDSTTELYHFIGKDIVYFHSLFWPAMLDGAGFRKPNNV
FVHGYVTVNGAKMSKSKGTFIKAGTYLNHLDPECLRYYYAAKLNSRIDDLDLNLEDFTQRVNSDVVNKIVNLASRNAGFI
TKRFDGKLADNFVEPELYNEFIAAADRIAELYETREFGRAIREITALADKANQYIDEKAPWVLAKEEGKEQELQEVSSVG
INLFRVLMAYLKPVMPELAARTEAFLNETLTWEGVAQPLVAHEITKFKALFARIDPKKVEAMIEESKEDAAIEMAAKEKA
EAEKEKASQTELDKDPIADEIEFDAFEAVDMRIARIISCEEVPKANKLLKFQLDIGGETRQVFSGIKSAYKPEELEGKLT
VMVANLKPRKMKFGMSEGMILAAGPGGKELWILEPHEGAQPGMRVM
>Mature_685_residues
ATDPRKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNDVNFICADDAHGTPIMLKAQQMGISPEEMIAAVSEEH
QKDFAGFDISFDNYHSTHSDENRELASHIYLELKKNGFITSRTISQLFDPEKEMFLPDRFVKGTCPKCKAEDQYGDNCDN
CGETYSPTDLINPKSAVSGATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQSETANKMQEWFESGLQQWDISRDAPYFGF
EIPGETNKFFYVWLDAPIGYMGSFKNLCNKRDDLNFDEYWKKDSTTELYHFIGKDIVYFHSLFWPAMLDGAGFRKPNNVF
VHGYVTVNGAKMSKSKGTFIKAGTYLNHLDPECLRYYYAAKLNSRIDDLDLNLEDFTQRVNSDVVNKIVNLASRNAGFIT
KRFDGKLADNFVEPELYNEFIAAADRIAELYETREFGRAIREITALADKANQYIDEKAPWVLAKEEGKEQELQEVSSVGI
NLFRVLMAYLKPVMPELAARTEAFLNETLTWEGVAQPLVAHEITKFKALFARIDPKKVEAMIEESKEDAAIEMAAKEKAE
AEKEKASQTELDKDPIADEIEFDAFEAVDMRIARIISCEEVPKANKLLKFQLDIGGETRQVFSGIKSAYKPEELEGKLTV
MVANLKPRKMKFGMSEGMILAAGPGGKELWILEPHEGAQPGMRVM

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=556, Percent_Identity=29.4964028776978, Blast_Score=265, Evalue=1e-70,
Organism=Homo sapiens, GI24308436, Length=530, Percent_Identity=26.9811320754717, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI4507947, Length=100, Percent_Identity=38, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788432, Length=687, Percent_Identity=69.5778748180495, Blast_Score=1031, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=588, Percent_Identity=30.4421768707483, Blast_Score=267, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI212645096, Length=515, Percent_Identity=24.2718446601942, Blast_Score=152, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71992781, Length=410, Percent_Identity=23.6585365853659, Blast_Score=109, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=559, Percent_Identity=29.5169946332737, Blast_Score=259, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6321610, Length=550, Percent_Identity=22.3636363636364, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24655311, Length=598, Percent_Identity=29.933110367893, Blast_Score=262, Evalue=8e-70,
Organism=Drosophila melanogaster, GI21358483, Length=514, Percent_Identity=25.2918287937743, Blast_Score=151, Evalue=2e-36,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 77888; Mature: 77757

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDPRKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNDVNFICADDAHGTPIM
CCCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEE
LKAQQMGISPEEMIAAVSEEHQKDFAGFDISFDNYHSTHSDENRELASHIYLELKKNGFI
EEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHEEEEECCCCC
TSRTISQLFDPEKEMFLPDRFVKGTCPKCKAEDQYGDNCDNCGETYSPTDLINPKSAVSG
HHHHHHHHHCCCHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
ATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQSETANKMQEWFESGLQQWDISRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPGETNKFFYVWLDAPIGYMGSFKNLCNKRDDLNFDEYWKKDSTTELYHFIGKDIVYF
EECCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
HSLFWPAMLDGAGFRKPNNVFVHGYVTVNGAKMSKSKGTFIKAGTYLNHLDPECLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEECCHHHCCCHHHHHHHHH
AKLNSRIDDLDLNLEDFTQRVNSDVVNKIVNLASRNAGFITKRFDGKLADNFVEPELYNE
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHCCCHHHHHH
FIAAADRIAELYETREFGRAIREITALADKANQYIDEKAPWVLAKEEGKEQELQEVSSVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHH
INLFRVLMAYLKPVMPELAARTEAFLNETLTWEGVAQPLVAHEITKFKALFARIDPKKVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AMIEESKEDAAIEMAAKEKAEAEKEKASQTELDKDPIADEIEFDAFEAVDMRIARIISCE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EVPKANKLLKFQLDIGGETRQVFSGIKSAYKPEELEGKLTVMVANLKPRKMKFGMSEGMI
HCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHCCCCCCCEE
LAAGPGGKELWILEPHEGAQPGMRVM
EEECCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ATDPRKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNDVNFICADDAHGTPIM
CCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEE
LKAQQMGISPEEMIAAVSEEHQKDFAGFDISFDNYHSTHSDENRELASHIYLELKKNGFI
EEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHEEEEECCCCC
TSRTISQLFDPEKEMFLPDRFVKGTCPKCKAEDQYGDNCDNCGETYSPTDLINPKSAVSG
HHHHHHHHHCCCHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
ATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQSETANKMQEWFESGLQQWDISRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPGETNKFFYVWLDAPIGYMGSFKNLCNKRDDLNFDEYWKKDSTTELYHFIGKDIVYF
EECCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
HSLFWPAMLDGAGFRKPNNVFVHGYVTVNGAKMSKSKGTFIKAGTYLNHLDPECLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEECCHHHCCCHHHHHHHHH
AKLNSRIDDLDLNLEDFTQRVNSDVVNKIVNLASRNAGFITKRFDGKLADNFVEPELYNE
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHCCCHHHHHH
FIAAADRIAELYETREFGRAIREITALADKANQYIDEKAPWVLAKEEGKEQELQEVSSVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHH
INLFRVLMAYLKPVMPELAARTEAFLNETLTWEGVAQPLVAHEITKFKALFARIDPKKVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AMIEESKEDAAIEMAAKEKAEAEKEKASQTELDKDPIADEIEFDAFEAVDMRIARIISCE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EVPKANKLLKFQLDIGGETRQVFSGIKSAYKPEELEGKLTVMVANLKPRKMKFGMSEGMI
HCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHCCCCCCCEE
LAAGPGGKELWILEPHEGAQPGMRVM
EEECCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA