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The map label for this gene is menC

Identifier: 59712274

GI number: 59712274

Start: 1877142

End: 1878116

Strand: Reverse

Name: menC

Synonym: VF_1667

Alternate gene names: 59712274

Gene position: 1878116-1877142 (Counterclockwise)

Preceding gene: 172087717

Following gene: 59712273

Centisome position: 64.82

GC content: 40.1

Gene sequence:

>975_bases
ATGAAAACAGCCAAGATCTATCAATACCAACTCCCTATGGACAGTGGAGTAATTCTTCGTGAACAACGTCTTCAGCAAAG
AGATGGATTGGTCATTGAGTTAAGTGATGGAATACATACAGCAAGAGGTGAGGTAGCACCTTTACCTGAATTCAGCCAAG
AAACGCTAGAACAAGCTCGTGAAGATTTAATTTCATTAACTCAATCATGGTTAAACAATGAAGAGTTGGATCTTGATTCA
AACTGCCCATCGGTTGCATTTGGCTTTAGCATGGCTTTGTTAGAACTTGAAAAACAGTTGCCACAAGAAGGTAATTACCA
AGCAGCACCTTTGTGCTCTGGTGATCCTGATGACTTGGTTGTTAAGCTTAATGAAATGAGCGGTAAGAAAATTGCCAAAA
TCAAAGTGGGTTTATACGAACCTATTCGTGATGGCATGGTTGTGAATATGTTTTTAGAACTGATTTCTGATCTCTCTTTA
CGTCTTGATGCTAACCGTGGCTGGACAGCAAAGAAAGCAGAGCAATTTGCTAATTACGTACATCCACAATTTCGTTCTCG
TATCGAATTCTTAGAAGAACCATGTGCTACACCAGAAGAAAGTTTAGCTTTCTCTAAAGCCACCGATATTGCAATCGCAT
GGGATGAAACTGTACGTGATGATGGTTTTACTGTTAAAACTCAAGAAGGCGTTACAGCTATTGTCATTAAGCCAACATTA
GTGGGTTCTGTTGAAAAGTGTATTTCGTTAATAGAACAAGCACATCAATTAGGCATGCAAGCGGTGATCAGCTCAAGCAT
TGAATCAAGTTTGGCATTAACACAACTTGCACGATTAGCTGCTTGGAAAACACCAGAAACGATTCCGGGTTTAGATACTA
TCGATCTGTTTAAAATGCAGCTAGATACTTCTTGGCCTAATTGCGACTTACCAGTGGCACAATTGGCTGATCTCGAGGCG
ATATGGGAAAACTGA

Upstream 100 bases:

>100_bases
CGATAAGTTCCCTCGTAACCCATAATTCGCTGTTACAGATTCAATTAAGTATTAGAGGCTCCTTTGAGTCTCTTTTTTCT
TTTATTGGTTGGAGTAAGAG

Downstream 100 bases:

>100_bases
TCTCACCAGCATTCACGACTTGGCCTTGGGTTAAGTGGAGTGAAGAGCGAGAATTAGACATTGCATTGCGTGATGGCGAT
AAAGTGTGGACATGGAGTGA

Product: O-succinylbenzoate synthase

Products: NA

Alternate protein names: OSB synthase; OSBS; 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; o-succinylbenzoic acid synthase

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKTAKIYQYQLPMDSGVILREQRLQQRDGLVIELSDGIHTARGEVAPLPEFSQETLEQAREDLISLTQSWLNNEELDLDS
NCPSVAFGFSMALLELEKQLPQEGNYQAAPLCSGDPDDLVVKLNEMSGKKIAKIKVGLYEPIRDGMVVNMFLELISDLSL
RLDANRGWTAKKAEQFANYVHPQFRSRIEFLEEPCATPEESLAFSKATDIAIAWDETVRDDGFTVKTQEGVTAIVIKPTL
VGSVEKCISLIEQAHQLGMQAVISSSIESSLALTQLARLAAWKTPETIPGLDTIDLFKMQLDTSWPNCDLPVAQLADLEA
IWEN

Sequences:

>Translated_324_residues
MKTAKIYQYQLPMDSGVILREQRLQQRDGLVIELSDGIHTARGEVAPLPEFSQETLEQAREDLISLTQSWLNNEELDLDS
NCPSVAFGFSMALLELEKQLPQEGNYQAAPLCSGDPDDLVVKLNEMSGKKIAKIKVGLYEPIRDGMVVNMFLELISDLSL
RLDANRGWTAKKAEQFANYVHPQFRSRIEFLEEPCATPEESLAFSKATDIAIAWDETVRDDGFTVKTQEGVTAIVIKPTL
VGSVEKCISLIEQAHQLGMQAVISSSIESSLALTQLARLAAWKTPETIPGLDTIDLFKMQLDTSWPNCDLPVAQLADLEA
IWEN
>Mature_324_residues
MKTAKIYQYQLPMDSGVILREQRLQQRDGLVIELSDGIHTARGEVAPLPEFSQETLEQAREDLISLTQSWLNNEELDLDS
NCPSVAFGFSMALLELEKQLPQEGNYQAAPLCSGDPDDLVVKLNEMSGKKIAKIKVGLYEPIRDGMVVNMFLELISDLSL
RLDANRGWTAKKAEQFANYVHPQFRSRIEFLEEPCATPEESLAFSKATDIAIAWDETVRDDGFTVKTQEGVTAIVIKPTL
VGSVEKCISLIEQAHQLGMQAVISSSIESSLALTQLARLAAWKTPETIPGLDTIDLFKMQLDTSWPNCDLPVAQLADLEA
IWEN

Specific function: Converts SHCHC to OSB

COG id: COG1441

COG function: function code H; O-succinylbenzoate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1788596, Length=322, Percent_Identity=57.1428571428571, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENC_VIBF1 (Q5E484)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205050.1
- HSSP:   P29208
- ProteinModelPortal:   Q5E484
- SMR:   Q5E484
- STRING:   Q5E484
- GeneID:   3278951
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1667
- NMPDR:   fig|312309.3.peg.1667
- eggNOG:   COG1441
- HOGENOM:   HBG644552
- OMA:   YEANRDG
- PhylomeDB:   Q5E484
- ProtClustDB:   PRK05105
- BioCyc:   VFIS312309:VF1667-MONOMER
- HAMAP:   MF_00470
- InterPro:   IPR013342
- InterPro:   IPR010196
- SMART:   SM00922
- TIGRFAMs:   TIGR01927

Pfam domain/function: PF01188 MR_MLE

EC number: =4.2.1.113

Molecular weight: Translated: 36055; Mature: 36055

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: NA

Important sites: ACT_SITE 135-135 ACT_SITE 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAKIYQYQLPMDSGVILREQRLQQRDGLVIELSDGIHTARGEVAPLPEFSQETLEQAR
CCCCEEEEEECCCCCCEEHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
EDLISLTQSWLNNEELDLDSNCPSVAFGFSMALLELEKQLPQEGNYQAAPLCSGDPDDLV
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
VKLNEMSGKKIAKIKVGLYEPIRDGMVVNMFLELISDLSLRLDANRGWTAKKAEQFANYV
EEEECCCCCEEEEEEECCHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHH
HPQFRSRIEFLEEPCATPEESLAFSKATDIAIAWDETVRDDGFTVKTQEGVTAIVIKPTL
CHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCEEEEECCHH
VGSVEKCISLIEQAHQLGMQAVISSSIESSLALTQLARLAAWKTPETIPGLDTIDLFKMQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEE
LDTSWPNCDLPVAQLADLEAIWEN
ECCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTAKIYQYQLPMDSGVILREQRLQQRDGLVIELSDGIHTARGEVAPLPEFSQETLEQAR
CCCCEEEEEECCCCCCEEHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
EDLISLTQSWLNNEELDLDSNCPSVAFGFSMALLELEKQLPQEGNYQAAPLCSGDPDDLV
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
VKLNEMSGKKIAKIKVGLYEPIRDGMVVNMFLELISDLSLRLDANRGWTAKKAEQFANYV
EEEECCCCCEEEEEEECCHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHH
HPQFRSRIEFLEEPCATPEESLAFSKATDIAIAWDETVRDDGFTVKTQEGVTAIVIKPTL
CHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCEEEEECCHH
VGSVEKCISLIEQAHQLGMQAVISSSIESSLALTQLARLAAWKTPETIPGLDTIDLFKMQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEE
LDTSWPNCDLPVAQLADLEAIWEN
ECCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA