Click here to switch to the map view.

The map label for this gene is menE [H]

Identifier: 59712273

GI number: 59712273

Start: 1875754

End: 1877154

Strand: Reverse

Name: menE [H]

Synonym: VF_1666

Alternate gene names: 59712273

Gene position: 1877154-1875754 (Counterclockwise)

Preceding gene: 59712274

Following gene: 59712270

Centisome position: 64.78

GC content: 42.83

Gene sequence:

>1401_bases
ATGGGAAAACTGATCTCACCAGCATTCACGACTTGGCCTTGGGTTAAGTGGAGTGAAGAGCGAGAATTAGACATTGCATT
GCGTGATGGCGATAAAGTGTGGACATGGAGTGAAGTGTCTACACAAATCAATCGTTATGCTAATGGTCTTCTTCAGCAAG
GCGTTAAGCGTGATGATGTTATTGTTGGCATTGCTAAAAACTGCATAGAATTAGTTTGGTTGCAGCTGGCCTCTGTTCGT
ATTGGAGCTCGCTTTGCCGCAATTAATCCAAAGACACCGAGCAAACAGCTGCACCAATTAGTTACTAGCATTGCTGCTAA
TCATATTTGGTTTGGTGAAGGTCAAGAGACCATCTCTGGTACATGGCATCATTTGCAGCTGATAAACGCAGAATATGGTG
CAGTAGCGGCTACATGGCAACCAACTCGCTTAGCAACATTAACGTTTACATCAGGCTCAACGGGAAACCCTAAAGCTGTC
GCGCACAGTGCAAGTAACCACTTACACTCAGCCGCTGGTTTATTAAGTTGGTTACACTTTGATGTCAGTGATTCTTGGCT
GGTATCACTGCCTTTGTTTCATGTTTCAGGCTTAGCTATTGTTTGGCGTTGGTTACTTTCTGGCGGAATGCTGGTTATGC
CAACAGAGCAAGGCTTGCTGAGTGATTTAGCTGGTGTTACGCATGCGTCGTTAGTTCCAACTCAACTGCAACGTATCTTA
GATAGTGGTGAAACATCTCAGCTTGCATTAAAACGTGTTCTACTTGGTGGTAGTGTTATTCCAACCGAATTAACAATAGC
TGCGCAAAAACAGGGCATAGAATGTTGGCTTGGTTATGGTATGACCGAAATGGGCTCAACCGTAACAGTAAAAAGAGCAG
ATGGTGAGCCGGGAGTAGGGAATGTTCTGCCTTATCGAGAATTACGCATTGAAGAAGGCGAAGTGTGGGTAAAAGGAGAG
AGTTTAACTCTAGGTTATTATCGCTCTTCAGGCATTATTGATGTAGCAAACGAAGATGGTTGGTTTGAGACCAAAGATCT
TGGTTCTGTGATTAATGCTGAGCTCCATATTCATGGACGTGCTGATAACATGTTTATTTCTGGTGGCGAAAATATCCATC
CTGAAAAAATAGAGCAAGTGCTACTGACACATCATGAGATCGATAATGTGATTGTTGTCGATGTGCCTGATGAGGAGTAT
GGACAACGACCTGTTGCTGTTCTTGTTTCAAACACAGGCAAAATTCCTGCTAAGTTAGTCGAGTTTACTCAAGATAAATT
GGCTAAATTTGAGCAACCGATAAGTTATGAAATCATGCCGGAACATTTGCTTATTTCTGGCATTAAAATTTCACGTAAAT
TGGTTAAAGAGTGGGTTGATTCACTGCCAATCACCGCGTAA

Upstream 100 bases:

>100_bases
TTCCGGGTTTAGATACTATCGATCTGTTTAAAATGCAGCTAGATACTTCTTGGCCTAATTGCGACTTACCAGTGGCACAA
TTGGCTGATCTCGAGGCGAT

Downstream 100 bases:

>100_bases
TTGAGCTACTTTTTCACGCATCAGTTTTGATGTCGCATCTTGTGGTAGTACGGGTTCTCCTGCTACCACCTCTACTTTAG
ACCAACAGCGACTAGGGCGT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MGKLISPAFTTWPWVKWSEERELDIALRDGDKVWTWSEVSTQINRYANGLLQQGVKRDDVIVGIAKNCIELVWLQLASVR
IGARFAAINPKTPSKQLHQLVTSIAANHIWFGEGQETISGTWHHLQLINAEYGAVAATWQPTRLATLTFTSGSTGNPKAV
AHSASNHLHSAAGLLSWLHFDVSDSWLVSLPLFHVSGLAIVWRWLLSGGMLVMPTEQGLLSDLAGVTHASLVPTQLQRIL
DSGETSQLALKRVLLGGSVIPTELTIAAQKQGIECWLGYGMTEMGSTVTVKRADGEPGVGNVLPYRELRIEEGEVWVKGE
SLTLGYYRSSGIIDVANEDGWFETKDLGSVINAELHIHGRADNMFISGGENIHPEKIEQVLLTHHEIDNVIVVDVPDEEY
GQRPVAVLVSNTGKIPAKLVEFTQDKLAKFEQPISYEIMPEHLLISGIKISRKLVKEWVDSLPITA

Sequences:

>Translated_466_residues
MGKLISPAFTTWPWVKWSEERELDIALRDGDKVWTWSEVSTQINRYANGLLQQGVKRDDVIVGIAKNCIELVWLQLASVR
IGARFAAINPKTPSKQLHQLVTSIAANHIWFGEGQETISGTWHHLQLINAEYGAVAATWQPTRLATLTFTSGSTGNPKAV
AHSASNHLHSAAGLLSWLHFDVSDSWLVSLPLFHVSGLAIVWRWLLSGGMLVMPTEQGLLSDLAGVTHASLVPTQLQRIL
DSGETSQLALKRVLLGGSVIPTELTIAAQKQGIECWLGYGMTEMGSTVTVKRADGEPGVGNVLPYRELRIEEGEVWVKGE
SLTLGYYRSSGIIDVANEDGWFETKDLGSVINAELHIHGRADNMFISGGENIHPEKIEQVLLTHHEIDNVIVVDVPDEEY
GQRPVAVLVSNTGKIPAKLVEFTQDKLAKFEQPISYEIMPEHLLISGIKISRKLVKEWVDSLPITA
>Mature_465_residues
GKLISPAFTTWPWVKWSEERELDIALRDGDKVWTWSEVSTQINRYANGLLQQGVKRDDVIVGIAKNCIELVWLQLASVRI
GARFAAINPKTPSKQLHQLVTSIAANHIWFGEGQETISGTWHHLQLINAEYGAVAATWQPTRLATLTFTSGSTGNPKAVA
HSASNHLHSAAGLLSWLHFDVSDSWLVSLPLFHVSGLAIVWRWLLSGGMLVMPTEQGLLSDLAGVTHASLVPTQLQRILD
SGETSQLALKRVLLGGSVIPTELTIAAQKQGIECWLGYGMTEMGSTVTVKRADGEPGVGNVLPYRELRIEEGEVWVKGES
LTLGYYRSSGIIDVANEDGWFETKDLGSVINAELHIHGRADNMFISGGENIHPEKIEQVLLTHHEIDNVIVVDVPDEEYG
QRPVAVLVSNTGKIPAKLVEFTQDKLAKFEQPISYEIMPEHLLISGIKISRKLVKEWVDSLPITA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=529, Percent_Identity=23.062381852552, Blast_Score=90, Evalue=6e-18,
Organism=Escherichia coli, GI1788595, Length=452, Percent_Identity=44.4690265486726, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI145693145, Length=343, Percent_Identity=25.6559766763848, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI221142682, Length=482, Percent_Identity=23.4439834024896, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788107, Length=335, Percent_Identity=26.2686567164179, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786810, Length=501, Percent_Identity=23.5528942115768, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789201, Length=272, Percent_Identity=27.2058823529412, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=457, Percent_Identity=26.6958424507659, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=439, Percent_Identity=26.879271070615, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=364, Percent_Identity=28.021978021978, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=495, Percent_Identity=23.030303030303, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17557194, Length=492, Percent_Identity=23.5772357723577, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=472, Percent_Identity=23.5169491525424, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71994690, Length=472, Percent_Identity=23.5169491525424, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=472, Percent_Identity=23.5169491525424, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=360, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71996755, Length=423, Percent_Identity=23.1678486997636, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=377, Percent_Identity=24.6684350132626, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24581924, Length=331, Percent_Identity=26.2839879154079, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21355181, Length=458, Percent_Identity=24.6724890829694, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51342; Mature: 51211

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLISPAFTTWPWVKWSEERELDIALRDGDKVWTWSEVSTQINRYANGLLQQGVKRDDV
CCCCCCCCCCCCCCEEECCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCE
IVGIAKNCIELVWLQLASVRIGARFAAINPKTPSKQLHQLVTSIAANHIWFGEGQETISG
EEEHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCEEECCCCHHHCC
TWHHLQLINAEYGAVAATWQPTRLATLTFTSGSTGNPKAVAHSASNHLHSAAGLLSWLHF
CEEEEEEEECCCCEEEEECCCCEEEEEEEECCCCCCCHHEEECHHHHHHHHHHHHHHHHC
DVSDSWLVSLPLFHVSGLAIVWRWLLSGGMLVMPTEQGLLSDLAGVTHASLVPTQLQRIL
CCCCCEEEEEHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
DSGETSQLALKRVLLGGSVIPTELTIAAQKQGIECWLGYGMTEMGSTVTVKRADGEPGVG
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCCHHHCCCEEEEEECCCCCCCC
NVLPYRELRIEEGEVWVKGESLTLGYYRSSGIIDVANEDGWFETKDLGSVINAELHIHGR
CCCCCHHEEEECCCEEEECCEEEEEEEECCCEEEECCCCCCEEHHHHHHHEEEEEEEEEC
ADNMFISGGENIHPEKIEQVLLTHHEIDNVIVVDVPDEEYGQRPVAVLVSNTGKIPAKLV
CCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCEEEEEECCCCCHHHHH
EFTQDKLAKFEQPISYEIMPEHLLISGIKISRKLVKEWVDSLPITA
HHHHHHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GKLISPAFTTWPWVKWSEERELDIALRDGDKVWTWSEVSTQINRYANGLLQQGVKRDDV
CCCCCCCCCCCCCEEECCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCE
IVGIAKNCIELVWLQLASVRIGARFAAINPKTPSKQLHQLVTSIAANHIWFGEGQETISG
EEEHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCEEECCCCHHHCC
TWHHLQLINAEYGAVAATWQPTRLATLTFTSGSTGNPKAVAHSASNHLHSAAGLLSWLHF
CEEEEEEEECCCCEEEEECCCCEEEEEEEECCCCCCCHHEEECHHHHHHHHHHHHHHHHC
DVSDSWLVSLPLFHVSGLAIVWRWLLSGGMLVMPTEQGLLSDLAGVTHASLVPTQLQRIL
CCCCCEEEEEHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
DSGETSQLALKRVLLGGSVIPTELTIAAQKQGIECWLGYGMTEMGSTVTVKRADGEPGVG
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCCHHHCCCEEEEEECCCCCCCC
NVLPYRELRIEEGEVWVKGESLTLGYYRSSGIIDVANEDGWFETKDLGSVINAELHIHGR
CCCCCHHEEEECCCEEEECCEEEEEEEECCCEEEECCCCCCEEHHHHHHHEEEEEEEEEC
ADNMFISGGENIHPEKIEQVLLTHHEIDNVIVVDVPDEEYGQRPVAVLVSNTGKIPAKLV
CCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCEEEEEECCCCCHHHHH
EFTQDKLAKFEQPISYEIMPEHLLISGIKISRKLVKEWVDSLPITA
HHHHHHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]