Click here to switch to the map view.

The map label for this gene is ansA [H]

Identifier: 59712251

GI number: 59712251

Start: 1850444

End: 1851457

Strand: Direct

Name: ansA [H]

Synonym: VF_1644

Alternate gene names: 59712251

Gene position: 1850444-1851457 (Clockwise)

Preceding gene: 59712250

Following gene: 59712253

Centisome position: 63.86

GC content: 41.22

Gene sequence:

>1014_bases
ATGGAAAGAAAACACATTTACATCGCCTATACTGGCGGCACAATCGGCATGATAAAGTCTGATGATCATGGTTATGTTCC
AGCTGCTGGATTCATGCAACATCAACTTAAACAAATGCCAGAGTTCCATCGTGCAGAAATGCCAATTTTCACCATTCACG
AATACGAGCCTTTGATTGACTCTTCAGACATGACGCCTGAAGACTGGCAACGTATTGCTGACGATATTCGTGCTAATTAC
GACAAATACGACGGATTTGTGATTCTTCATGGTACGGACACCATGGCCTACACAGCATCGGCTCTTTCTTTCATGCTAGA
GAACCTTGGAAAACCAGTTATTGTTACTGGCTCTCAAATTCCTCTTGCAGAACTTCGCTCTGATGGCCAAAGCAACTTAT
TAAATGCACTGCATATCGCAGCAAATTACCCTATCAATGAAGTAACGTTATTCTTTAATAACAAATTGATCCGTGGTAAT
CGCAGTACTAAGTCACATGCAGATGGTTTTGATGCGTTTTCTTCACCAAACTTACCACCTTTATTAGAGGCTGGTATTAA
TATTCAAATTAATGGTGCAGAAATCAACAAACAGCCTGAAGGTGAGTTCACAGTAAATAACATTACACCACAACCAATCG
GTGTCGTGACAATGTATCCTGGGATCTCGGCAGAAGTGATCAAAAATACACTACGCCAACCAGTCAATGCGATGATCTTA
CTGACCTTTGGTGTGGGTAATGCGCCACAAAACCCTGAGCTTCTTGCGCAATTAAAAGAAGCATCTGAACGTGGCGTTGT
CGTGGTTAACTTGACTCAATGTTTATCTGGAAAAGTAAACATGGGTGGCTATGCAACAGGCTGTGCTCTAGCTGAAGCTG
GAGTAATTAGTGGTTATGATATGACACCAGAAGCTGCTCTAGCTAAACTTCATTATATTCTAAGCAAAGAATTACCGTAT
GAAACCATGCGTACTATGATGCAACAGAATTTATGTGGTGAACTAACGCATTAA

Upstream 100 bases:

>100_bases
ATCTTTAAAAGCATGACGAGCCCGACACATGTCGGGCTTTCTTTATGCCAAATGCACAGTATAATCCCCCCACCTCCCAT
AACTCTGTACCGTTGTTATT

Downstream 100 bases:

>100_bases
TTTCGATTTACGTAATACAAAAAAGGCTTCCCTGAATTCTCATGGGAAGCCTTTTTTAATTCTAGATAATTAACCGTAAA
TTATCTTTTTTCGTTCAAAA

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MERKHIYIAYTGGTIGMIKSDDHGYVPAAGFMQHQLKQMPEFHRAEMPIFTIHEYEPLIDSSDMTPEDWQRIADDIRANY
DKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQSNLLNALHIAANYPINEVTLFFNNKLIRGN
RSTKSHADGFDAFSSPNLPPLLEAGINIQINGAEINKQPEGEFTVNNITPQPIGVVTMYPGISAEVIKNTLRQPVNAMIL
LTFGVGNAPQNPELLAQLKEASERGVVVVNLTQCLSGKVNMGGYATGCALAEAGVISGYDMTPEAALAKLHYILSKELPY
ETMRTMMQQNLCGELTH

Sequences:

>Translated_337_residues
MERKHIYIAYTGGTIGMIKSDDHGYVPAAGFMQHQLKQMPEFHRAEMPIFTIHEYEPLIDSSDMTPEDWQRIADDIRANY
DKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQSNLLNALHIAANYPINEVTLFFNNKLIRGN
RSTKSHADGFDAFSSPNLPPLLEAGINIQINGAEINKQPEGEFTVNNITPQPIGVVTMYPGISAEVIKNTLRQPVNAMIL
LTFGVGNAPQNPELLAQLKEASERGVVVVNLTQCLSGKVNMGGYATGCALAEAGVISGYDMTPEAALAKLHYILSKELPY
ETMRTMMQQNLCGELTH
>Mature_337_residues
MERKHIYIAYTGGTIGMIKSDDHGYVPAAGFMQHQLKQMPEFHRAEMPIFTIHEYEPLIDSSDMTPEDWQRIADDIRANY
DKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQSNLLNALHIAANYPINEVTLFFNNKLIRGN
RSTKSHADGFDAFSSPNLPPLLEAGINIQINGAEINKQPEGEFTVNNITPQPIGVVTMYPGISAEVIKNTLRQPVNAMIL
LTFGVGNAPQNPELLAQLKEASERGVVVVNLTQCLSGKVNMGGYATGCALAEAGVISGYDMTPEAALAKLHYILSKELPY
ETMRTMMQQNLCGELTH

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=359, Percent_Identity=45.125348189415, Blast_Score=279, Evalue=2e-75,
Organism=Escherichia coli, GI1788065, Length=336, Percent_Identity=74.7023809523809, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1789327, Length=283, Percent_Identity=26.5017667844523, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI193207372, Length=361, Percent_Identity=47.0914127423823, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17558280, Length=361, Percent_Identity=47.0914127423823, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17558278, Length=361, Percent_Identity=47.0914127423823, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI193207374, Length=361, Percent_Identity=47.0914127423823, Blast_Score=311, Evalue=4e-85,
Organism=Saccharomyces cerevisiae, GI6320527, Length=180, Percent_Identity=30, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24639609, Length=364, Percent_Identity=43.6813186813187, Blast_Score=298, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24645530, Length=359, Percent_Identity=45.125348189415, Blast_Score=291, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36989; Mature: 36989

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2 ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKHIYIAYTGGTIGMIKSDDHGYVPAAGFMQHQLKQMPEFHRAEMPIFTIHEYEPLID
CCCCEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCC
SSDMTPEDWQRIADDIRANYDKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQI
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCC
PLAELRSDGQSNLLNALHIAANYPINEVTLFFNNKLIRGNRSTKSHADGFDAFSSPNLPP
CHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCH
LLEAGINIQINGAEINKQPEGEFTVNNITPQPIGVVTMYPGISAEVIKNTLRQPVNAMIL
HHHCCCEEEEECCCCCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEE
LTFGVGNAPQNPELLAQLKEASERGVVVVNLTQCLSGKVNMGGYATGCALAEAGVISGYD
EEECCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCCCHHHHHHHCCCCCCCC
MTPEAALAKLHYILSKELPYETMRTMMQQNLCGELTH
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MERKHIYIAYTGGTIGMIKSDDHGYVPAAGFMQHQLKQMPEFHRAEMPIFTIHEYEPLID
CCCCEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCC
SSDMTPEDWQRIADDIRANYDKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQI
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCC
PLAELRSDGQSNLLNALHIAANYPINEVTLFFNNKLIRGNRSTKSHADGFDAFSSPNLPP
CHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCH
LLEAGINIQINGAEINKQPEGEFTVNNITPQPIGVVTMYPGISAEVIKNTLRQPVNAMIL
HHHCCCEEEEECCCCCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEE
LTFGVGNAPQNPELLAQLKEASERGVVVVNLTQCLSGKVNMGGYATGCALAEAGVISGYD
EEECCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCCCHHHHHHHCCCCCCCC
MTPEAALAKLHYILSKELPYETMRTMMQQNLCGELTH
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]