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The map label for this gene is mleN [H]

Identifier: 59712253

GI number: 59712253

Start: 1853278

End: 1854714

Strand: Direct

Name: mleN [H]

Synonym: VF_1646

Alternate gene names: 59712253

Gene position: 1853278-1854714 (Clockwise)

Preceding gene: 59712251

Following gene: 59712256

Centisome position: 63.96

GC content: 40.71

Gene sequence:

>1437_bases
ATGAAGCAAGCAACAACAAAGTTACCTTCGATTACGCAGGTAATTATATCTTTAGGGCTATTTCTACTTCTTGCCTTTTC
ATTCACGGCACAACTTGACCTACCTATTCAACTAGCTCTTTACATCGGCTGGTTCATTATCATGACGTTAGGTATCAAAT
TAGGCCATGATTATAAATCTTTAGAAAAAGCAGCTCTAAATGGCATCTCCAATGGACTAGGAGCAGTATTAATTCTCTTG
GCTGTTGGCGCATTAGTGGGTACTTGGATCTCTGGTGGTATTGTTCCAACCATTATTTACTACGGCCTAAAAGCCATTCA
CCCATCTATCTTCCTTTTAGCTACAATGATCATCTGTTCATTAACGGCTCTTGCAACAGGAACATCATGGGGTGCGGCTG
GTACAGCTGGTATTGCAATGATGGGGATCGGTCAAGGTCTTGGTGTTCCAGCACCAATTACTGCTGGTGCAATCCTTTCA
GGCTGTTACTTTGGCGACAAAATGTCTCCACTTTCAGATTCTGTAATTCTTGCTTCTTCAATGTCTAATGTTGAAGTTAT
GGAGCACATTAAAGGCATGCTGCCTGTTGCTCTTATCAGCTACATCATCACTGGTATTATGTTTACTGCATTTGGTTTCC
ACTTTGCAGGTAACGTAGACATGAGCCAAGTTGATTCTGTTATCTTAGCAATGGAAGAGCAATTTGTTATCTCTCCATTC
TCATTTGTTCCTGTTGTTATTGTTCTTGGCCTATTGGCGATGAGAATGCCTTCTTTCCCAGTGATCAGCTTTGGTTCTCT
TTTGGGTATTGTATGGGCTGTAATGGTACAAGATATCGATTTTTTGACAGCATTTAATACTGCATGGGCACCTTTCTCAA
TTTCATCTGGTGTTGAGTTTATTGATGCGATCCTAAACCGTGGTGGTATGTCTTCAATGCTTGGCTCAGTTGCAGTTATC
GTATTTGGTCTAGGTTTTGGTGGTCTATTAGATAAAGTAGGTGTTCTTGAAACCGTTGCTAAACTATTTGAAAAACGAGT
TCAAGGCCCAGGTTCTCTTGCAACAAGTACGATTGCGACTGCTTTCTTTGGTAACATCTTCGGTTCGGCGATGTATGTAT
CGCTTATCCTTACACCAAAGATTTGTGCGAAAAACTACGACCGTTTAGGTTACAAACGTAAGAACCTATCTCGTAATGCT
GAGTTTGGTGGTACGTTAACTTCAGGTATGGTTCCTTGGAGTGATAATGGTATCTACATGGCAAGCATCTTAGGCGTAGC
TACACTGTCTTATGCACCATTCATGTGGTTAAGCTTCGTATGTATTATCGTAACGATTGTAACTTCATACTTCGGTTGGT
TTGTTGATAAATGCGAACCAACAGTAACGGTAGAAGAAACTGATGAAGCTATCGCTGTAGAAACAAAAACAGCATAA

Upstream 100 bases:

>100_bases
CGATCTATTGTTGATCATAAGGTAAAGTGCACCTCGTTTAGAAAACACAACCCCTTATCGTATGAATTTTATTCATACAT
ACAAAAAAGAGGCTTTATAC

Downstream 100 bases:

>100_bases
TTTCTTAGTTTACTCATTAAATAAAAATGCCAGTGATTGATCACTGGCATTTTTGTATCTAGCACTTAATTATTCGGATT
ATGTTTATTATACCATTCTG

Product: malate-2-proton/lactate-sodium antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKQATTKLPSITQVIISLGLFLLLAFSFTAQLDLPIQLALYIGWFIIMTLGIKLGHDYKSLEKAALNGISNGLGAVLILL
AVGALVGTWISGGIVPTIIYYGLKAIHPSIFLLATMIICSLTALATGTSWGAAGTAGIAMMGIGQGLGVPAPITAGAILS
GCYFGDKMSPLSDSVILASSMSNVEVMEHIKGMLPVALISYIITGIMFTAFGFHFAGNVDMSQVDSVILAMEEQFVISPF
SFVPVVIVLGLLAMRMPSFPVISFGSLLGIVWAVMVQDIDFLTAFNTAWAPFSISSGVEFIDAILNRGGMSSMLGSVAVI
VFGLGFGGLLDKVGVLETVAKLFEKRVQGPGSLATSTIATAFFGNIFGSAMYVSLILTPKICAKNYDRLGYKRKNLSRNA
EFGGTLTSGMVPWSDNGIYMASILGVATLSYAPFMWLSFVCIIVTIVTSYFGWFVDKCEPTVTVEETDEAIAVETKTA

Sequences:

>Translated_478_residues
MKQATTKLPSITQVIISLGLFLLLAFSFTAQLDLPIQLALYIGWFIIMTLGIKLGHDYKSLEKAALNGISNGLGAVLILL
AVGALVGTWISGGIVPTIIYYGLKAIHPSIFLLATMIICSLTALATGTSWGAAGTAGIAMMGIGQGLGVPAPITAGAILS
GCYFGDKMSPLSDSVILASSMSNVEVMEHIKGMLPVALISYIITGIMFTAFGFHFAGNVDMSQVDSVILAMEEQFVISPF
SFVPVVIVLGLLAMRMPSFPVISFGSLLGIVWAVMVQDIDFLTAFNTAWAPFSISSGVEFIDAILNRGGMSSMLGSVAVI
VFGLGFGGLLDKVGVLETVAKLFEKRVQGPGSLATSTIATAFFGNIFGSAMYVSLILTPKICAKNYDRLGYKRKNLSRNA
EFGGTLTSGMVPWSDNGIYMASILGVATLSYAPFMWLSFVCIIVTIVTSYFGWFVDKCEPTVTVEETDEAIAVETKTA
>Mature_478_residues
MKQATTKLPSITQVIISLGLFLLLAFSFTAQLDLPIQLALYIGWFIIMTLGIKLGHDYKSLEKAALNGISNGLGAVLILL
AVGALVGTWISGGIVPTIIYYGLKAIHPSIFLLATMIICSLTALATGTSWGAAGTAGIAMMGIGQGLGVPAPITAGAILS
GCYFGDKMSPLSDSVILASSMSNVEVMEHIKGMLPVALISYIITGIMFTAFGFHFAGNVDMSQVDSVILAMEEQFVISPF
SFVPVVIVLGLLAMRMPSFPVISFGSLLGIVWAVMVQDIDFLTAFNTAWAPFSISSGVEFIDAILNRGGMSSMLGSVAVI
VFGLGFGGLLDKVGVLETVAKLFEKRVQGPGSLATSTIATAFFGNIFGSAMYVSLILTPKICAKNYDRLGYKRKNLSRNA
EFGGTLTSGMVPWSDNGIYMASILGVATLSYAPFMWLSFVCIIVTIVTSYFGWFVDKCEPTVTVEETDEAIAVETKTA

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 50644; Mature: 50644

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQATTKLPSITQVIISLGLFLLLAFSFTAQLDLPIQLALYIGWFIIMTLGIKLGHDYKS
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
LEKAALNGISNGLGAVLILLAVGALVGTWISGGIVPTIIYYGLKAIHPSIFLLATMIICS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTALATGTSWGAAGTAGIAMMGIGQGLGVPAPITAGAILSGCYFGDKMSPLSDSVILASS
HHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEHHC
MSNVEVMEHIKGMLPVALISYIITGIMFTAFGFHFAGNVDMSQVDSVILAMEEQFVISPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCH
SFVPVVIVLGLLAMRMPSFPVISFGSLLGIVWAVMVQDIDFLTAFNTAWAPFSISSGVEF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHH
IDAILNRGGMSSMLGSVAVIVFGLGFGGLLDKVGVLETVAKLFEKRVQGPGSLATSTIAT
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AFFGNIFGSAMYVSLILTPKICAKNYDRLGYKRKNLSRNAEFGGTLTSGMVPWSDNGIYM
HHHHHHHHHHHHHHHHHCHHHHHCCHHHCCCHHHCCCCCCCCCCCHHCCCCCCCCCCCHH
ASILGVATLSYAPFMWLSFVCIIVTIVTSYFGWFVDKCEPTVTVEETDEAIAVETKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCC
>Mature Secondary Structure
MKQATTKLPSITQVIISLGLFLLLAFSFTAQLDLPIQLALYIGWFIIMTLGIKLGHDYKS
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
LEKAALNGISNGLGAVLILLAVGALVGTWISGGIVPTIIYYGLKAIHPSIFLLATMIICS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTALATGTSWGAAGTAGIAMMGIGQGLGVPAPITAGAILSGCYFGDKMSPLSDSVILASS
HHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEHHC
MSNVEVMEHIKGMLPVALISYIITGIMFTAFGFHFAGNVDMSQVDSVILAMEEQFVISPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCH
SFVPVVIVLGLLAMRMPSFPVISFGSLLGIVWAVMVQDIDFLTAFNTAWAPFSISSGVEF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHH
IDAILNRGGMSSMLGSVAVIVFGLGFGGLLDKVGVLETVAKLFEKRVQGPGSLATSTIAT
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AFFGNIFGSAMYVSLILTPKICAKNYDRLGYKRKNLSRNAEFGGTLTSGMVPWSDNGIYM
HHHHHHHHHHHHHHHHHCHHHHHCCHHHCCCHHHCCCCCCCCCCCHHCCCCCCCCCCCHH
ASILGVATLSYAPFMWLSFVCIIVTIVTSYFGWFVDKCEPTVTVEETDEAIAVETKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]