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The map label for this gene is folE [H]

Identifier: 59712214

GI number: 59712214

Start: 1803595

End: 1804248

Strand: Reverse

Name: folE [H]

Synonym: VF_1607

Alternate gene names: 59712214

Gene position: 1804248-1803595 (Counterclockwise)

Preceding gene: 59712221

Following gene: 59712212

Centisome position: 62.27

GC content: 39.91

Gene sequence:

>654_bases
ATGTCATCACTTAGTGAATCAGCCATATTAGTGCGTGATGCTCTTGTTGCACGTGGTCTTGAGACGCCAATGAAAGAAAA
TGGTGTTAGTCGAGAAGAGAAAAAAGAGCGCATCGAAGAGCATATGCGTGAGATCCTAACCCTATTAAGTCTTGATCTTA
GTGATGATAGTTTAGAAGAAACGCCACACCGTATTGCAAAAATGTATGTGGATGAGATTTTCTCTGGGCTAGATTATGCC
AATTTCCCTAAAATTACAGTGATTGAAAATAAGATGAACTGTGATGAGATGGTTCGTGTTAATGATATTACGTTAACCAG
TACTTGCGAACATCACTTAGTGACAATTGATGGTAAAGCGACGGTTGCTTATATTCCTCGTGGAAAGATCATCGGATTAT
CTAAAATTAACCGCATTGTTCGATTCTTCGCTCAACGTCCACAAGTACAAGAACGCATGACTCAGCAAATACTTGTGGCT
TTACAAACCTTATTAGGTAGTGACGATGTAGCTATTACTATGGAAGCAACGCACTATTGTGTTAAGTCTCGTGGTGTAAT
GGATGCAACAAGTAGTACAACAACGACTGCACTTGGTGGGATCTTTAAGAGAAACCCAGCGACAAGACACGAGTTTTTAA
GCGGTATTCGTTAA

Upstream 100 bases:

>100_bases
CTTTAACATCTAATAGACCTAGTAAAATCAGGGTGTATTTATTTGATAGAGGAGGTATCCTTAGCGAAGTTCAATTGATT
ATGGAATTTTGGAGTATACG

Downstream 100 bases:

>100_bases
ATACAGCCATCTTGTTAAATATCACAATAAAGTAAACATTAAAAAAGCCAGTGACCTTGGCAAAGAGGTCACTGGCTTTT
TCTTATGTGTTTTTAGAGTA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSSLSESAILVRDALVARGLETPMKENGVSREEKKERIEEHMREILTLLSLDLSDDSLEETPHRIAKMYVDEIFSGLDYA
NFPKITVIENKMNCDEMVRVNDITLTSTCEHHLVTIDGKATVAYIPRGKIIGLSKINRIVRFFAQRPQVQERMTQQILVA
LQTLLGSDDVAITMEATHYCVKSRGVMDATSSTTTTALGGIFKRNPATRHEFLSGIR

Sequences:

>Translated_217_residues
MSSLSESAILVRDALVARGLETPMKENGVSREEKKERIEEHMREILTLLSLDLSDDSLEETPHRIAKMYVDEIFSGLDYA
NFPKITVIENKMNCDEMVRVNDITLTSTCEHHLVTIDGKATVAYIPRGKIIGLSKINRIVRFFAQRPQVQERMTQQILVA
LQTLLGSDDVAITMEATHYCVKSRGVMDATSSTTTTALGGIFKRNPATRHEFLSGIR
>Mature_216_residues
SSLSESAILVRDALVARGLETPMKENGVSREEKKERIEEHMREILTLLSLDLSDDSLEETPHRIAKMYVDEIFSGLDYAN
FPKITVIENKMNCDEMVRVNDITLTSTCEHHLVTIDGKATVAYIPRGKIIGLSKINRIVRFFAQRPQVQERMTQQILVAL
QTLLGSDDVAITMEATHYCVKSRGVMDATSSTTTTALGGIFKRNPATRHEFLSGIR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=180, Percent_Identity=40.5555555555556, Blast_Score=124, Evalue=5e-29,
Organism=Homo sapiens, GI4503949, Length=180, Percent_Identity=40.5555555555556, Blast_Score=124, Evalue=5e-29,
Organism=Homo sapiens, GI66932970, Length=155, Percent_Identity=37.4193548387097, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI66932972, Length=140, Percent_Identity=39.2857142857143, Blast_Score=87, Evalue=9e-18,
Organism=Escherichia coli, GI1788476, Length=219, Percent_Identity=71.689497716895, Blast_Score=314, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17560486, Length=200, Percent_Identity=38.5, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321706, Length=181, Percent_Identity=43.0939226519337, Blast_Score=130, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24656796, Length=120, Percent_Identity=48.3333333333333, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656782, Length=120, Percent_Identity=48.3333333333333, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656791, Length=120, Percent_Identity=48.3333333333333, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 24355; Mature: 24224

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSESAILVRDALVARGLETPMKENGVSREEKKERIEEHMREILTLLSLDLSDDSLEE
CCCCHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TPHRIAKMYVDEIFSGLDYANFPKITVIENKMNCDEMVRVNDITLTSTCEHHLVTIDGKA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHEEECCEEEECCCCCEEEEECCCE
TVAYIPRGKIIGLSKINRIVRFFAQRPQVQERMTQQILVALQTLLGSDDVAITMEATHYC
EEEEECCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
VKSRGVMDATSSTTTTALGGIFKRNPATRHEFLSGIR
HHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
SSLSESAILVRDALVARGLETPMKENGVSREEKKERIEEHMREILTLLSLDLSDDSLEE
CCCHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TPHRIAKMYVDEIFSGLDYANFPKITVIENKMNCDEMVRVNDITLTSTCEHHLVTIDGKA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHEEECCEEEECCCCCEEEEECCCE
TVAYIPRGKIIGLSKINRIVRFFAQRPQVQERMTQQILVALQTLLGSDDVAITMEATHYC
EEEEECCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
VKSRGVMDATSSTTTTALGGIFKRNPATRHEFLSGIR
HHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA